Status
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Gene information
General information
TC030696 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9162766..9178112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030696
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02614

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: usually the head appendages and the prothoracic legs are affected
    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030696 RA
      MEEILSKLL VANSKVIQQ GTKELKEAF KKPEAIPAL 
      CEVIVSSSN PQIRQSAAV LLRRKLGKK RQWNKLDAE 
      TRSRIKQGM LQALVNEQE KLVKNAIAQ FIGILGKHE 
      FPENTWPEV LQFIHTLCS SDNVFDREL GMYTLSIMT 
      EISQGSYIT HADSFAVLF SNILNTLPE LNSNLAYYT 
      VVTMNNLVS VIGGHQQMV NVYHNLLPR VLEIINAFA 
      QEDEKRACE LFEILEELI EFAVAVVVP HVRLIVEMC 
      LRIGSDNTK PTTVQIKAI SVVGWLIRS KGKVIQKNK 
      LVEPIINVL IQLMAQQPD DDVNEEYFL GDPDQFTSI 
      TIATQTLDL IALHIPSEK VVPYLLTRV EPAIQGNDI 
      YAQKAAYLA LAVLAEGCS ERIRHKYLE PFLKCVCNA 
      IHNPNAVVR NAAFFALGQ FAEHLQPEI SQYAAELLP 
      VLFEYLGQV FAQMEKDKS ESASLDRLF YALETFCEN 
      LDEGLMPYL PTLMERLFV ALDPNGWSL KLKRIALST 
      LGSAASAVK EGLLPYFPK IIEVLNVYI NADPNTEIH 
      QNQSYAIEA LAVIAQFIG VENFKPLAA ESLQLGLRI 
      LEETDDPDV RKSVYALFA ALAIVMKEE ISPVLPKIV 
      EQMITSIQS SEGIVTHYE DEEKEDLDV YADLSDDDE 
      EEEEDIDGA SSSSADSTH CRYSVENSY NEEKEQACL 
      ALREICINT GNAFLPYIE KSFEEIFKL INYPQDDIR 
      KASVEALLQ FCIALHKIN SNETKQALY KALQMFVPK 
      CAELIRTDE ERGVVMCCL DAYASLLEE VKSDVLVGE 
      GHREAIMNC VIDVLTLKT MCQDTDLDA NPENTDEET 
      EAEQDELLL ESAGDVIPK FGAAITPDD FVLYFPNIL 
      QLLTQRTKK QHSVSQRSF AFGTLAECM KSLDVYVEK 
      FVQHLLHLW LTGAKDSAD EVRNNAIFG LGEMILHGK 
      DRIFGYFPD ILQALSTAV AKESHAGTL DNICGALAK 
      MIIVNPGGV PLDQVLPAF LQRLPLRDD FQENEAVVK 
      CFFTLYQQG NPILREHLS SVIKIVVQV YDKKQYPND 
      ETKNILVEL IKTINRDFS EEFGNTVSS LGPEVTKSL 
      QNLFATS
    CDS sequence
    • >TC030696 RA
      atggaagaa attttgtcc aaactactc gtggccaat 
      agcaaagtc attcaacag ggcaccaaa gaactgaag 
      gaggctttc aaaaaacca gaagcgatt cccgctctt 
      tgcgaagtg atagtttcg tcgtcaaat cctcaaatc 
      agacaatca gccgctgtg ctgctcagg agaaaactc 
      ggcaaaaaa cgacaatgg aacaaattg gatgcagaa 
      acgaggagt cgaatcaaa caagggatg ctgcaagcg 
      ttagtcaat gagcaagag aagttagtc aaaaatgca 
      attgcccag tttattggg attctaggc aagcatgag 
      tttccggaa aacacgtgg ccggaagtc ttgcaattt 
      atacacaca ctgtgcagc agcgacaat gttttcgac 
      cgggagttg gggatgtac accttgtcc ataatgact 
      gagatctcg caaggctct tacataacg cacgccgat 
      tcgtttgcg gtgctgttt tcgaatatt ttgaatact 
      cttcctgaa ttaaattcg aacctagct tactacaca 
      gtggttaca atgaataat ctagtgtct gtaataggc 
      ggccatcag caaatggta aacgtttac cataattta 
      ctgcctcgt gtattagaa ataatcaac gcttttgct 
      caagaagac gaaaaacgc gcttgcgaa ctatttgaa 
      atcctcgaa gagctgatc gagtttgcg gtcgcggtg 
      gtggtacca cacgtgcga ctgatcgta gaaatgtgc 
      cttcgcatc ggcagcgat aacaccaaa ccaacaaca 
      gtccaaatt aaagcaatt agtgttgtg ggctggtta 
      attaggtcc aagggcaag gttatccaa aaaaataaa 
      ctcgtcgaa ccaattatc aatgttttg atacaacta 
      atggcccaa cagccggac gacgacgtc aacgaggag 
      tacttccta ggggacccg gatcaattc acttccatt 
      actattgcc actcagact ctagacttg atcgctttg 
      cacatacct tcagagaag gttgttccg tatttactg 
      acaagagtg gaaccggcg attcaaggg aatgatatt 
      tatgcccaa aaagcggca tatttagcc ttggctgtg 
      ctcgcagaa gggtgctct gaacggatt agacacaaa 
      tatttagaa ccgttttta aaatgcgtc tgcaatgcg 
      attcataat ccgaatgct gtggtcaga aatgccgcg 
      tttttcgct cttgggcag ttcgccgag catttacag 
      ccggaaatc agccaatac gcggccgaa ctcctcccg 
      gtacttttc gagtacttg ggccaggtt ttcgcccaa 
      atggaaaaa gataaatcg gaatcggcc agtcttgat 
      cgacttttt tacgcattg gaaaccttc tgtgaaaac 
      ctcgatgag ggtttaatg ccctactta cccaccctt 
      atggaacgt ttgttcgtg gcacttgac ccaaacggc 
      tggtccttg aagctaaaa agaatcgct ttaagtact 
      ctaggttcc gcagctagt gctgtaaaa gagggttta 
      ttaccctac tttcccaaa ataatcgag gtgcttaac 
      gtttacatt aacgccgat ccgaatacg gaaatacat 
      caaaatcag agttacgca atagaggca cttgctgtg 
      attgcacaa tttatcggt gttgagaat ttcaagcct 
      ttggcggct gagtccttg caactcggg ttgcgaatt 
      ttggaggaa actgacgat ccagatgtt cgaaaaagt 
      gtctatgcg ttatttgca gcccttgcg attgttatg 
      aaggaggaa atcagtcca gttttgcca aaaattgtc 
      gaacagatg atcactagc atacagagt agtgagggc 
      attgtcaca cattatgaa gacgaggaa aaggaagat 
      ttggacgtt tatgccgac ctttcggac gacgatgaa 
      gaagaagag gaagatatc gatggagca tcgtccagt 
      agcgccgat tcgactcat tgtcgctat tcggtcgaa 
      aattcctac aacgaggaa aaagaacaa gcgtgcttg 
      gcacttagg gaaatttgc atcaacacg gggaatgcc 
      tttttgcca tatatcgag aaaagtttc gaggaaatc 
      ttcaaattg ataaattat ccacaggat gacatacgc 
      aaggcttcg gtcgaagcc ctcctccag ttttgcata 
      gctttacac aaaatcaac tcaaacgaa accaagcaa 
      gctctctat aaagcttta caaatgttt gtaccaaaa 
      tgtgccgaa cttatccga accgatgag gaacgaggc 
      gttgtcatg tgctgtttg gacgcttac gcttccctg 
      cttgaggaa gttaaaagt gacgttttg gtcggggaa 
      ggccatcgg gaggctatc atgaattgt gtcattgac 
      gtcctcacg ttaaaaaca atgtgtcaa gacacagat 
      ttggacgca aatcccgaa aatacagac gaggaaaca 
      gaggcggaa caagacgag ttgttgctt gaatcagcc 
      ggtgatgtt ataccgaag ttcggagcg gcgataact 
      cccgatgat tttgtttta tatttcccg aatattctc 
      caactgctg acgcagaga actaaaaag cagcacagt 
      gtgagccag cgttcgttc gcgttcggc actttggcc 
      gaatgcatg aaatcgctg gacgtctac gtcgaaaaa 
      ttcgttcag catttgctg catttgtgg ctgactggg 
      gccaaagat tcggccgac gaggtgcga aataacgcg 
      attttcggt cttggcgaa atgattttg cacgggaag 
      gaccgaatt ttcgggtat ttccccgat atactccag 
      gctttgtcc actgctgtg gctaaggag tcgcacgca 
      gggactctg gataatatc tgcggtgct ttagctaag 
      atgattatt gttaatcct gggggagtt cccctggat 
      caggtgctc ccagcgttc ctccaacgc ctgcctctc 
      agggacgat ttccaagag aacgaagct gtagttaag 
      tgtttcttt actttgtat caacagggg aatcccatc 
      ctgcgcgag catttatcc agtgttatc aaaattgtg 
      gttcaggtg tacgacaag aaacaatat ccgaatgat 
      gaaaccaaa aatatttta gtggaactg atcaaaacg 
      ataaatagg gattttagt gaagagttt gggaacaca 
      gtgtcttcc ttaggacca gaagtgact aaatcgtta 
      cagaatttg tttgctact tcttaa
    mRNA sequence
    • >TC030696 RA
      tgccaactg tcattttcc agatgattc acatcaatt 
      atcccagcc ccaacccaa tttttttac atcttttag 
      cataccaca ctccactgt gtatgtcat tcattcgaa 
      tcagcgacg atggaagaa attttgtcc aaactactc 
      gtggccaat agcaaagtc attcaacag ggcaccaaa 
      gaactgaag gaggctttc aaaaaacca gaagcgatt 
      cccgctctt tgcgaagtg atagtttcg tcgtcaaat 
      cctcaaatc agacaatca gccgctgtg ctgctcagg 
      agaaaactc ggcaaaaaa cgacaatgg aacaaattg 
      gatgcagaa acgaggagt cgaatcaaa caagggatg 
      ctgcaagcg ttagtcaat gagcaagag aagttagtc 
      aaaaatgca attgcccag tttattggg attctaggc 
      aagcatgag tttccggaa aacacgtgg ccggaagtc 
      ttgcaattt atacacaca ctgtgcagc agcgacaat 
      gttttcgac cgggagttg gggatgtac accttgtcc 
      ataatgact gagatctcg caaggctct tacataacg 
      cacgccgat tcgtttgcg gtgctgttt tcgaatatt 
      ttgaatact cttcctgaa ttaaattcg aacctagct 
      tactacaca gtggttaca atgaataat ctagtgtct 
      gtaataggc ggccatcag caaatggta aacgtttac 
      cataattta ctgcctcgt gtattagaa ataatcaac 
      gcttttgct caagaagac gaaaaacgc gcttgcgaa 
      ctatttgaa atcctcgaa gagctgatc gagtttgcg 
      gtcgcggtg gtggtacca cacgtgcga ctgatcgta 
      gaaatgtgc cttcgcatc ggcagcgat aacaccaaa 
      ccaacaaca gtccaaatt aaagcaatt agtgttgtg 
      ggctggtta attaggtcc aagggcaag gttatccaa 
      aaaaataaa ctcgtcgaa ccaattatc aatgttttg 
      atacaacta atggcccaa cagccggac gacgacgtc 
      aacgaggag tacttccta ggggacccg gatcaattc 
      acttccatt actattgcc actcagact ctagacttg 
      atcgctttg cacatacct tcagagaag gttgttccg 
      tatttactg acaagagtg gaaccggcg attcaaggg 
      aatgatatt tatgcccaa aaagcggca tatttagcc 
      ttggctgtg ctcgcagaa gggtgctct gaacggatt 
      agacacaaa tatttagaa ccgttttta aaatgcgtc 
      tgcaatgcg attcataat ccgaatgct gtggtcaga 
      aatgccgcg tttttcgct cttgggcag ttcgccgag 
      catttacag ccggaaatc agccaatac gcggccgaa 
      ctcctcccg gtacttttc gagtacttg ggccaggtt 
      ttcgcccaa atggaaaaa gataaatcg gaatcggcc 
      agtcttgat cgacttttt tacgcattg gaaaccttc 
      tgtgaaaac ctcgatgag ggtttaatg ccctactta 
      cccaccctt atggaacgt ttgttcgtg gcacttgac 
      ccaaacggc tggtccttg aagctaaaa agaatcgct 
      ttaagtact ctaggttcc gcagctagt gctgtaaaa 
      gagggttta ttaccctac tttcccaaa ataatcgag 
      gtgcttaac gtttacatt aacgccgat ccgaatacg 
      gaaatacat caaaatcag agttacgca atagaggca 
      cttgctgtg attgcacaa tttatcggt gttgagaat 
      ttcaagcct ttggcggct gagtccttg caactcggg 
      ttgcgaatt ttggaggaa actgacgat ccagatgtt 
      cgaaaaagt gtctatgcg ttatttgca gcccttgcg 
      attgttatg aaggaggaa atcagtcca gttttgcca 
      aaaattgtc gaacagatg atcactagc atacagagt 
      agtgagggc attgtcaca cattatgaa gacgaggaa 
      aaggaagat ttggacgtt tatgccgac ctttcggac 
      gacgatgaa gaagaagag gaagatatc gatggagca 
      tcgtccagt agcgccgat tcgactcat tgtcgctat 
      tcggtcgaa aattcctac aacgaggaa aaagaacaa 
      gcgtgcttg gcacttagg gaaatttgc atcaacacg 
      gggaatgcc tttttgcca tatatcgag aaaagtttc 
      gaggaaatc ttcaaattg ataaattat ccacaggat 
      gacatacgc aaggcttcg gtcgaagcc ctcctccag 
      ttttgcata gctttacac aaaatcaac tcaaacgaa 
      accaagcaa gctctctat aaagcttta caaatgttt 
      gtaccaaaa tgtgccgaa cttatccga accgatgag 
      gaacgaggc gttgtcatg tgctgtttg gacgcttac 
      gcttccctg cttgaggaa gttaaaagt gacgttttg 
      gtcggggaa ggccatcgg gaggctatc atgaattgt 
      gtcattgac gtcctcacg ttaaaaaca atgtgtcaa 
      gacacagat ttggacgca aatcccgaa aatacagac 
      gaggaaaca gaggcggaa caagacgag ttgttgctt 
      gaatcagcc ggtgatgtt ataccgaag ttcggagcg 
      gcgataact cccgatgat tttgtttta tatttcccg 
      aatattctc caactgctg acgcagaga actaaaaag 
      cagcacagt gtgagccag cgttcgttc gcgttcggc 
      actttggcc gaatgcatg aaatcgctg gacgtctac 
      gtcgaaaaa ttcgttcag catttgctg catttgtgg 
      ctgactggg gccaaagat tcggccgac gaggtgcga 
      aataacgcg attttcggt cttggcgaa atgattttg 
      cacgggaag gaccgaatt ttcgggtat ttccccgat 
      atactccag gctttgtcc actgctgtg gctaaggag 
      tcgcacgca gggactctg gataatatc tgcggtgct 
      ttagctaag atgattatt gttaatcct gggggagtt 
      cccctggat caggtgctc ccagcgttc ctccaacgc 
      ctgcctctc agggacgat ttccaagag aacgaagct 
      gtagttaag tgtttcttt actttgtat caacagggg 
      aatcccatc ctgcgcgag catttatcc agtgttatc 
      aaaattgtg gttcaggtg tacgacaag aaacaatat 
      ccgaatgat gaaaccaaa aatatttta gtggaactg 
      atcaaaacg ataaatagg gattttagt gaagagttt 
      gggaacaca gtgtcttcc ttaggacca gaagtgact 
      aaatcgtta cagaatttg tttgctact tcttaagac 
      caaaacctt aattcctgt cctggcaat ctcagaagt 
      ttttttatt ttttgggac atgtgttaa tgttttttg 
      ttgagtcat atttttgtt tatttattg tatctgtgt 
      atcgactat aataatgct gcatcttaa gagttttaa 
      agtgattac tactgtttt taataaaaa attgcttcg 
      gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY