General information | |||
TC030696 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 9162766..9178112 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC030696 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02614 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
Comment: usually the head appendages and the prothoracic legs are affected |
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC030696 RA
MEEILSKLL VANSKVIQQ GTKELKEAF KKPEAIPAL CEVIVSSSN PQIRQSAAV LLRRKLGKK RQWNKLDAE TRSRIKQGM LQALVNEQE KLVKNAIAQ FIGILGKHE FPENTWPEV LQFIHTLCS SDNVFDREL GMYTLSIMT EISQGSYIT HADSFAVLF SNILNTLPE LNSNLAYYT VVTMNNLVS VIGGHQQMV NVYHNLLPR VLEIINAFA QEDEKRACE LFEILEELI EFAVAVVVP HVRLIVEMC LRIGSDNTK PTTVQIKAI SVVGWLIRS KGKVIQKNK LVEPIINVL IQLMAQQPD DDVNEEYFL GDPDQFTSI TIATQTLDL IALHIPSEK VVPYLLTRV EPAIQGNDI YAQKAAYLA LAVLAEGCS ERIRHKYLE PFLKCVCNA IHNPNAVVR NAAFFALGQ FAEHLQPEI SQYAAELLP VLFEYLGQV FAQMEKDKS ESASLDRLF YALETFCEN LDEGLMPYL PTLMERLFV ALDPNGWSL KLKRIALST LGSAASAVK EGLLPYFPK IIEVLNVYI NADPNTEIH QNQSYAIEA LAVIAQFIG VENFKPLAA ESLQLGLRI LEETDDPDV RKSVYALFA ALAIVMKEE ISPVLPKIV EQMITSIQS SEGIVTHYE DEEKEDLDV YADLSDDDE EEEEDIDGA SSSSADSTH CRYSVENSY NEEKEQACL ALREICINT GNAFLPYIE KSFEEIFKL INYPQDDIR KASVEALLQ FCIALHKIN SNETKQALY KALQMFVPK CAELIRTDE ERGVVMCCL DAYASLLEE VKSDVLVGE GHREAIMNC VIDVLTLKT MCQDTDLDA NPENTDEET EAEQDELLL ESAGDVIPK FGAAITPDD FVLYFPNIL QLLTQRTKK QHSVSQRSF AFGTLAECM KSLDVYVEK FVQHLLHLW LTGAKDSAD EVRNNAIFG LGEMILHGK DRIFGYFPD ILQALSTAV AKESHAGTL DNICGALAK MIIVNPGGV PLDQVLPAF LQRLPLRDD FQENEAVVK CFFTLYQQG NPILREHLS SVIKIVVQV YDKKQYPND ETKNILVEL IKTINRDFS EEFGNTVSS LGPEVTKSL QNLFATS
- >TC030696 RA
atggaagaa attttgtcc aaactactc gtggccaat agcaaagtc attcaacag ggcaccaaa gaactgaag gaggctttc aaaaaacca gaagcgatt cccgctctt tgcgaagtg atagtttcg tcgtcaaat cctcaaatc agacaatca gccgctgtg ctgctcagg agaaaactc ggcaaaaaa cgacaatgg aacaaattg gatgcagaa acgaggagt cgaatcaaa caagggatg ctgcaagcg ttagtcaat gagcaagag aagttagtc aaaaatgca attgcccag tttattggg attctaggc aagcatgag tttccggaa aacacgtgg ccggaagtc ttgcaattt atacacaca ctgtgcagc agcgacaat gttttcgac cgggagttg gggatgtac accttgtcc ataatgact gagatctcg caaggctct tacataacg cacgccgat tcgtttgcg gtgctgttt tcgaatatt ttgaatact cttcctgaa ttaaattcg aacctagct tactacaca gtggttaca atgaataat ctagtgtct gtaataggc ggccatcag caaatggta aacgtttac cataattta ctgcctcgt gtattagaa ataatcaac gcttttgct caagaagac gaaaaacgc gcttgcgaa ctatttgaa atcctcgaa gagctgatc gagtttgcg gtcgcggtg gtggtacca cacgtgcga ctgatcgta gaaatgtgc cttcgcatc ggcagcgat aacaccaaa ccaacaaca gtccaaatt aaagcaatt agtgttgtg ggctggtta attaggtcc aagggcaag gttatccaa aaaaataaa ctcgtcgaa ccaattatc aatgttttg atacaacta atggcccaa cagccggac gacgacgtc aacgaggag tacttccta ggggacccg gatcaattc acttccatt actattgcc actcagact ctagacttg atcgctttg cacatacct tcagagaag gttgttccg tatttactg acaagagtg gaaccggcg attcaaggg aatgatatt tatgcccaa aaagcggca tatttagcc ttggctgtg ctcgcagaa gggtgctct gaacggatt agacacaaa tatttagaa ccgttttta aaatgcgtc tgcaatgcg attcataat ccgaatgct gtggtcaga aatgccgcg tttttcgct cttgggcag ttcgccgag catttacag ccggaaatc agccaatac gcggccgaa ctcctcccg gtacttttc gagtacttg ggccaggtt ttcgcccaa atggaaaaa gataaatcg gaatcggcc agtcttgat cgacttttt tacgcattg gaaaccttc tgtgaaaac ctcgatgag ggtttaatg ccctactta cccaccctt atggaacgt ttgttcgtg gcacttgac ccaaacggc tggtccttg aagctaaaa agaatcgct ttaagtact ctaggttcc gcagctagt gctgtaaaa gagggttta ttaccctac tttcccaaa ataatcgag gtgcttaac gtttacatt aacgccgat ccgaatacg gaaatacat caaaatcag agttacgca atagaggca cttgctgtg attgcacaa tttatcggt gttgagaat ttcaagcct ttggcggct gagtccttg caactcggg ttgcgaatt ttggaggaa actgacgat ccagatgtt cgaaaaagt gtctatgcg ttatttgca gcccttgcg attgttatg aaggaggaa atcagtcca gttttgcca aaaattgtc gaacagatg atcactagc atacagagt agtgagggc attgtcaca cattatgaa gacgaggaa aaggaagat ttggacgtt tatgccgac ctttcggac gacgatgaa gaagaagag gaagatatc gatggagca tcgtccagt agcgccgat tcgactcat tgtcgctat tcggtcgaa aattcctac aacgaggaa aaagaacaa gcgtgcttg gcacttagg gaaatttgc atcaacacg gggaatgcc tttttgcca tatatcgag aaaagtttc gaggaaatc ttcaaattg ataaattat ccacaggat gacatacgc aaggcttcg gtcgaagcc ctcctccag ttttgcata gctttacac aaaatcaac tcaaacgaa accaagcaa gctctctat aaagcttta caaatgttt gtaccaaaa tgtgccgaa cttatccga accgatgag gaacgaggc gttgtcatg tgctgtttg gacgcttac gcttccctg cttgaggaa gttaaaagt gacgttttg gtcggggaa ggccatcgg gaggctatc atgaattgt gtcattgac gtcctcacg ttaaaaaca atgtgtcaa gacacagat ttggacgca aatcccgaa aatacagac gaggaaaca gaggcggaa caagacgag ttgttgctt gaatcagcc ggtgatgtt ataccgaag ttcggagcg gcgataact cccgatgat tttgtttta tatttcccg aatattctc caactgctg acgcagaga actaaaaag cagcacagt gtgagccag cgttcgttc gcgttcggc actttggcc gaatgcatg aaatcgctg gacgtctac gtcgaaaaa ttcgttcag catttgctg catttgtgg ctgactggg gccaaagat tcggccgac gaggtgcga aataacgcg attttcggt cttggcgaa atgattttg cacgggaag gaccgaatt ttcgggtat ttccccgat atactccag gctttgtcc actgctgtg gctaaggag tcgcacgca gggactctg gataatatc tgcggtgct ttagctaag atgattatt gttaatcct gggggagtt cccctggat caggtgctc ccagcgttc ctccaacgc ctgcctctc agggacgat ttccaagag aacgaagct gtagttaag tgtttcttt actttgtat caacagggg aatcccatc ctgcgcgag catttatcc agtgttatc aaaattgtg gttcaggtg tacgacaag aaacaatat ccgaatgat gaaaccaaa aatatttta gtggaactg atcaaaacg ataaatagg gattttagt gaagagttt gggaacaca gtgtcttcc ttaggacca gaagtgact aaatcgtta cagaatttg tttgctact tcttaa
- >TC030696 RA
tgccaactg tcattttcc agatgattc acatcaatt atcccagcc ccaacccaa tttttttac atcttttag cataccaca ctccactgt gtatgtcat tcattcgaa tcagcgacg atggaagaa attttgtcc aaactactc gtggccaat agcaaagtc attcaacag ggcaccaaa gaactgaag gaggctttc aaaaaacca gaagcgatt cccgctctt tgcgaagtg atagtttcg tcgtcaaat cctcaaatc agacaatca gccgctgtg ctgctcagg agaaaactc ggcaaaaaa cgacaatgg aacaaattg gatgcagaa acgaggagt cgaatcaaa caagggatg ctgcaagcg ttagtcaat gagcaagag aagttagtc aaaaatgca attgcccag tttattggg attctaggc aagcatgag tttccggaa aacacgtgg ccggaagtc ttgcaattt atacacaca ctgtgcagc agcgacaat gttttcgac cgggagttg gggatgtac accttgtcc ataatgact gagatctcg caaggctct tacataacg cacgccgat tcgtttgcg gtgctgttt tcgaatatt ttgaatact cttcctgaa ttaaattcg aacctagct tactacaca gtggttaca atgaataat ctagtgtct gtaataggc ggccatcag caaatggta aacgtttac cataattta ctgcctcgt gtattagaa ataatcaac gcttttgct caagaagac gaaaaacgc gcttgcgaa ctatttgaa atcctcgaa gagctgatc gagtttgcg gtcgcggtg gtggtacca cacgtgcga ctgatcgta gaaatgtgc cttcgcatc ggcagcgat aacaccaaa ccaacaaca gtccaaatt aaagcaatt agtgttgtg ggctggtta attaggtcc aagggcaag gttatccaa aaaaataaa ctcgtcgaa ccaattatc aatgttttg atacaacta atggcccaa cagccggac gacgacgtc aacgaggag tacttccta ggggacccg gatcaattc acttccatt actattgcc actcagact ctagacttg atcgctttg cacatacct tcagagaag gttgttccg tatttactg acaagagtg gaaccggcg attcaaggg aatgatatt tatgcccaa aaagcggca tatttagcc ttggctgtg ctcgcagaa gggtgctct gaacggatt agacacaaa tatttagaa ccgttttta aaatgcgtc tgcaatgcg attcataat ccgaatgct gtggtcaga aatgccgcg tttttcgct cttgggcag ttcgccgag catttacag ccggaaatc agccaatac gcggccgaa ctcctcccg gtacttttc gagtacttg ggccaggtt ttcgcccaa atggaaaaa gataaatcg gaatcggcc agtcttgat cgacttttt tacgcattg gaaaccttc tgtgaaaac ctcgatgag ggtttaatg ccctactta cccaccctt atggaacgt ttgttcgtg gcacttgac ccaaacggc tggtccttg aagctaaaa agaatcgct ttaagtact ctaggttcc gcagctagt gctgtaaaa gagggttta ttaccctac tttcccaaa ataatcgag gtgcttaac gtttacatt aacgccgat ccgaatacg gaaatacat caaaatcag agttacgca atagaggca cttgctgtg attgcacaa tttatcggt gttgagaat ttcaagcct ttggcggct gagtccttg caactcggg ttgcgaatt ttggaggaa actgacgat ccagatgtt cgaaaaagt gtctatgcg ttatttgca gcccttgcg attgttatg aaggaggaa atcagtcca gttttgcca aaaattgtc gaacagatg atcactagc atacagagt agtgagggc attgtcaca cattatgaa gacgaggaa aaggaagat ttggacgtt tatgccgac ctttcggac gacgatgaa gaagaagag gaagatatc gatggagca tcgtccagt agcgccgat tcgactcat tgtcgctat tcggtcgaa aattcctac aacgaggaa aaagaacaa gcgtgcttg gcacttagg gaaatttgc atcaacacg gggaatgcc tttttgcca tatatcgag aaaagtttc gaggaaatc ttcaaattg ataaattat ccacaggat gacatacgc aaggcttcg gtcgaagcc ctcctccag ttttgcata gctttacac aaaatcaac tcaaacgaa accaagcaa gctctctat aaagcttta caaatgttt gtaccaaaa tgtgccgaa cttatccga accgatgag gaacgaggc gttgtcatg tgctgtttg gacgcttac gcttccctg cttgaggaa gttaaaagt gacgttttg gtcggggaa ggccatcgg gaggctatc atgaattgt gtcattgac gtcctcacg ttaaaaaca atgtgtcaa gacacagat ttggacgca aatcccgaa aatacagac gaggaaaca gaggcggaa caagacgag ttgttgctt gaatcagcc ggtgatgtt ataccgaag ttcggagcg gcgataact cccgatgat tttgtttta tatttcccg aatattctc caactgctg acgcagaga actaaaaag cagcacagt gtgagccag cgttcgttc gcgttcggc actttggcc gaatgcatg aaatcgctg gacgtctac gtcgaaaaa ttcgttcag catttgctg catttgtgg ctgactggg gccaaagat tcggccgac gaggtgcga aataacgcg attttcggt cttggcgaa atgattttg cacgggaag gaccgaatt ttcgggtat ttccccgat atactccag gctttgtcc actgctgtg gctaaggag tcgcacgca gggactctg gataatatc tgcggtgct ttagctaag atgattatt gttaatcct gggggagtt cccctggat caggtgctc ccagcgttc ctccaacgc ctgcctctc agggacgat ttccaagag aacgaagct gtagttaag tgtttcttt actttgtat caacagggg aatcccatc ctgcgcgag catttatcc agtgttatc aaaattgtg gttcaggtg tacgacaag aaacaatat ccgaatgat gaaaccaaa aatatttta gtggaactg atcaaaacg ataaatagg gattttagt gaagagttt gggaacaca gtgtcttcc ttaggacca gaagtgact aaatcgtta cagaatttg tttgctact tcttaagac caaaacctt aattcctgt cctggcaat ctcagaagt ttttttatt ttttgggac atgtgttaa tgttttttg ttgagtcat atttttgtt tatttattg tatctgtgt atcgactat aataatgct gcatcttaa gagttttaa agtgattac tactgtttt taataaaaa attgcttcg gtt
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
