Status
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Gene information
General information
TC030696 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 9162766..9178112
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC030696
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02614

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    Comment: usually the head appendages and the prothoracic legs are affected
    head partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030696 RA
      MEEILSKLL VANSKVIQQ GTKELKEAF KKPEAIPAL 
CEVIVSSSN PQIRQSAAV LLRRKLGKK RQWNKLDAE 
TRSRIKQGM LQALVNEQE KLVKNAIAQ FIGILGKHE 
FPENTWPEV LQFIHTLCS SDNVFDREL GMYTLSIMT 
EISQGSYIT HADSFAVLF SNILNTLPE LNSNLAYYT 
VVTMNNLVS VIGGHQQMV NVYHNLLPR VLEIINAFA 
QEDEKRACE LFEILEELI EFAVAVVVP HVRLIVEMC 
LRIGSDNTK PTTVQIKAI SVVGWLIRS KGKVIQKNK 
LVEPIINVL IQLMAQQPD DDVNEEYFL GDPDQFTSI 
TIATQTLDL IALHIPSEK VVPYLLTRV EPAIQGNDI 
YAQKAAYLA LAVLAEGCS ERIRHKYLE PFLKCVCNA 
IHNPNAVVR NAAFFALGQ FAEHLQPEI SQYAAELLP 
VLFEYLGQV FAQMEKDKS ESASLDRLF YALETFCEN 
LDEGLMPYL PTLMERLFV ALDPNGWSL KLKRIALST 
LGSAASAVK EGLLPYFPK IIEVLNVYI NADPNTEIH 
QNQSYAIEA LAVIAQFIG VENFKPLAA ESLQLGLRI 
LEETDDPDV RKSVYALFA ALAIVMKEE ISPVLPKIV 
EQMITSIQS SEGIVTHYE DEEKEDLDV YADLSDDDE 
EEEEDIDGA SSSSADSTH CRYSVENSY NEEKEQACL 
ALREICINT GNAFLPYIE KSFEEIFKL INYPQDDIR 
KASVEALLQ FCIALHKIN SNETKQALY KALQMFVPK 
CAELIRTDE ERGVVMCCL DAYASLLEE VKSDVLVGE 
GHREAIMNC VIDVLTLKT MCQDTDLDA NPENTDEET 
EAEQDELLL ESAGDVIPK FGAAITPDD FVLYFPNIL 
QLLTQRTKK QHSVSQRSF AFGTLAECM KSLDVYVEK 
FVQHLLHLW LTGAKDSAD EVRNNAIFG LGEMILHGK 
DRIFGYFPD ILQALSTAV AKESHAGTL DNICGALAK 
MIIVNPGGV PLDQVLPAF LQRLPLRDD FQENEAVVK 
CFFTLYQQG NPILREHLS SVIKIVVQV YDKKQYPND 
ETKNILVEL IKTINRDFS EEFGNTVSS LGPEVTKSL 
QNLFATS
    CDS sequence
    • >TC030696 RA
      atggaagaa attttgtcc aaactactc gtggccaat 
agcaaagtc attcaacag ggcaccaaa gaactgaag 
gaggctttc aaaaaacca gaagcgatt cccgctctt 
tgcgaagtg atagtttcg tcgtcaaat cctcaaatc 
agacaatca gccgctgtg ctgctcagg agaaaactc 
ggcaaaaaa cgacaatgg aacaaattg gatgcagaa 
acgaggagt cgaatcaaa caagggatg ctgcaagcg 
ttagtcaat gagcaagag aagttagtc aaaaatgca 
attgcccag tttattggg attctaggc aagcatgag 
tttccggaa aacacgtgg ccggaagtc ttgcaattt 
atacacaca ctgtgcagc agcgacaat gttttcgac 
cgggagttg gggatgtac accttgtcc ataatgact 
gagatctcg caaggctct tacataacg cacgccgat 
tcgtttgcg gtgctgttt tcgaatatt ttgaatact 
cttcctgaa ttaaattcg aacctagct tactacaca 
gtggttaca atgaataat ctagtgtct gtaataggc 
ggccatcag caaatggta aacgtttac cataattta 
ctgcctcgt gtattagaa ataatcaac gcttttgct 
caagaagac gaaaaacgc gcttgcgaa ctatttgaa 
atcctcgaa gagctgatc gagtttgcg gtcgcggtg 
gtggtacca cacgtgcga ctgatcgta gaaatgtgc 
cttcgcatc ggcagcgat aacaccaaa ccaacaaca 
gtccaaatt aaagcaatt agtgttgtg ggctggtta 
attaggtcc aagggcaag gttatccaa aaaaataaa 
ctcgtcgaa ccaattatc aatgttttg atacaacta 
atggcccaa cagccggac gacgacgtc aacgaggag 
tacttccta ggggacccg gatcaattc acttccatt 
actattgcc actcagact ctagacttg atcgctttg 
cacatacct tcagagaag gttgttccg tatttactg 
acaagagtg gaaccggcg attcaaggg aatgatatt 
tatgcccaa aaagcggca tatttagcc ttggctgtg 
ctcgcagaa gggtgctct gaacggatt agacacaaa 
tatttagaa ccgttttta aaatgcgtc tgcaatgcg 
attcataat ccgaatgct gtggtcaga aatgccgcg 
tttttcgct cttgggcag ttcgccgag catttacag 
ccggaaatc agccaatac gcggccgaa ctcctcccg 
gtacttttc gagtacttg ggccaggtt ttcgcccaa 
atggaaaaa gataaatcg gaatcggcc agtcttgat 
cgacttttt tacgcattg gaaaccttc tgtgaaaac 
ctcgatgag ggtttaatg ccctactta cccaccctt 
atggaacgt ttgttcgtg gcacttgac ccaaacggc 
tggtccttg aagctaaaa agaatcgct ttaagtact 
ctaggttcc gcagctagt gctgtaaaa gagggttta 
ttaccctac tttcccaaa ataatcgag gtgcttaac 
gtttacatt aacgccgat ccgaatacg gaaatacat 
caaaatcag agttacgca atagaggca cttgctgtg 
attgcacaa tttatcggt gttgagaat ttcaagcct 
ttggcggct gagtccttg caactcggg ttgcgaatt 
ttggaggaa actgacgat ccagatgtt cgaaaaagt 
gtctatgcg ttatttgca gcccttgcg attgttatg 
aaggaggaa atcagtcca gttttgcca aaaattgtc 
gaacagatg atcactagc atacagagt agtgagggc 
attgtcaca cattatgaa gacgaggaa aaggaagat 
ttggacgtt tatgccgac ctttcggac gacgatgaa 
gaagaagag gaagatatc gatggagca tcgtccagt 
agcgccgat tcgactcat tgtcgctat tcggtcgaa 
aattcctac aacgaggaa aaagaacaa gcgtgcttg 
gcacttagg gaaatttgc atcaacacg gggaatgcc 
tttttgcca tatatcgag aaaagtttc gaggaaatc 
ttcaaattg ataaattat ccacaggat gacatacgc 
aaggcttcg gtcgaagcc ctcctccag ttttgcata 
gctttacac aaaatcaac tcaaacgaa accaagcaa 
gctctctat aaagcttta caaatgttt gtaccaaaa 
tgtgccgaa cttatccga accgatgag gaacgaggc 
gttgtcatg tgctgtttg gacgcttac gcttccctg 
cttgaggaa gttaaaagt gacgttttg gtcggggaa 
ggccatcgg gaggctatc atgaattgt gtcattgac 
gtcctcacg ttaaaaaca atgtgtcaa gacacagat 
ttggacgca aatcccgaa aatacagac gaggaaaca 
gaggcggaa caagacgag ttgttgctt gaatcagcc 
ggtgatgtt ataccgaag ttcggagcg gcgataact 
cccgatgat tttgtttta tatttcccg aatattctc 
caactgctg acgcagaga actaaaaag cagcacagt 
gtgagccag cgttcgttc gcgttcggc actttggcc 
gaatgcatg aaatcgctg gacgtctac gtcgaaaaa 
ttcgttcag catttgctg catttgtgg ctgactggg 
gccaaagat tcggccgac gaggtgcga aataacgcg 
attttcggt cttggcgaa atgattttg cacgggaag 
gaccgaatt ttcgggtat ttccccgat atactccag 
gctttgtcc actgctgtg gctaaggag tcgcacgca 
gggactctg gataatatc tgcggtgct ttagctaag 
atgattatt gttaatcct gggggagtt cccctggat 
caggtgctc ccagcgttc ctccaacgc ctgcctctc 
agggacgat ttccaagag aacgaagct gtagttaag 
tgtttcttt actttgtat caacagggg aatcccatc 
ctgcgcgag catttatcc agtgttatc aaaattgtg 
gttcaggtg tacgacaag aaacaatat ccgaatgat 
gaaaccaaa aatatttta gtggaactg atcaaaacg 
ataaatagg gattttagt gaagagttt gggaacaca 
gtgtcttcc ttaggacca gaagtgact aaatcgtta 
cagaatttg tttgctact tcttaa
    mRNA sequence
    • >TC030696 RA
      tgccaactg tcattttcc agatgattc acatcaatt 
atcccagcc ccaacccaa tttttttac atcttttag 
cataccaca ctccactgt gtatgtcat tcattcgaa 
tcagcgacg atggaagaa attttgtcc aaactactc 
gtggccaat agcaaagtc attcaacag ggcaccaaa 
gaactgaag gaggctttc aaaaaacca gaagcgatt 
cccgctctt tgcgaagtg atagtttcg tcgtcaaat 
cctcaaatc agacaatca gccgctgtg ctgctcagg 
agaaaactc ggcaaaaaa cgacaatgg aacaaattg 
gatgcagaa acgaggagt cgaatcaaa caagggatg 
ctgcaagcg ttagtcaat gagcaagag aagttagtc 
aaaaatgca attgcccag tttattggg attctaggc 
aagcatgag tttccggaa aacacgtgg ccggaagtc 
ttgcaattt atacacaca ctgtgcagc agcgacaat 
gttttcgac cgggagttg gggatgtac accttgtcc 
ataatgact gagatctcg caaggctct tacataacg 
cacgccgat tcgtttgcg gtgctgttt tcgaatatt 
ttgaatact cttcctgaa ttaaattcg aacctagct 
tactacaca gtggttaca atgaataat ctagtgtct 
gtaataggc ggccatcag caaatggta aacgtttac 
cataattta ctgcctcgt gtattagaa ataatcaac 
gcttttgct caagaagac gaaaaacgc gcttgcgaa 
ctatttgaa atcctcgaa gagctgatc gagtttgcg 
gtcgcggtg gtggtacca cacgtgcga ctgatcgta 
gaaatgtgc cttcgcatc ggcagcgat aacaccaaa 
ccaacaaca gtccaaatt aaagcaatt agtgttgtg 
ggctggtta attaggtcc aagggcaag gttatccaa 
aaaaataaa ctcgtcgaa ccaattatc aatgttttg 
atacaacta atggcccaa cagccggac gacgacgtc 
aacgaggag tacttccta ggggacccg gatcaattc 
acttccatt actattgcc actcagact ctagacttg 
atcgctttg cacatacct tcagagaag gttgttccg 
tatttactg acaagagtg gaaccggcg attcaaggg 
aatgatatt tatgcccaa aaagcggca tatttagcc 
ttggctgtg ctcgcagaa gggtgctct gaacggatt 
agacacaaa tatttagaa ccgttttta aaatgcgtc 
tgcaatgcg attcataat ccgaatgct gtggtcaga 
aatgccgcg tttttcgct cttgggcag ttcgccgag 
catttacag ccggaaatc agccaatac gcggccgaa 
ctcctcccg gtacttttc gagtacttg ggccaggtt 
ttcgcccaa atggaaaaa gataaatcg gaatcggcc 
agtcttgat cgacttttt tacgcattg gaaaccttc 
tgtgaaaac ctcgatgag ggtttaatg ccctactta 
cccaccctt atggaacgt ttgttcgtg gcacttgac 
ccaaacggc tggtccttg aagctaaaa agaatcgct 
ttaagtact ctaggttcc gcagctagt gctgtaaaa 
gagggttta ttaccctac tttcccaaa ataatcgag 
gtgcttaac gtttacatt aacgccgat ccgaatacg 
gaaatacat caaaatcag agttacgca atagaggca 
cttgctgtg attgcacaa tttatcggt gttgagaat 
ttcaagcct ttggcggct gagtccttg caactcggg 
ttgcgaatt ttggaggaa actgacgat ccagatgtt 
cgaaaaagt gtctatgcg ttatttgca gcccttgcg 
attgttatg aaggaggaa atcagtcca gttttgcca 
aaaattgtc gaacagatg atcactagc atacagagt 
agtgagggc attgtcaca cattatgaa gacgaggaa 
aaggaagat ttggacgtt tatgccgac ctttcggac 
gacgatgaa gaagaagag gaagatatc gatggagca 
tcgtccagt agcgccgat tcgactcat tgtcgctat 
tcggtcgaa aattcctac aacgaggaa aaagaacaa 
gcgtgcttg gcacttagg gaaatttgc atcaacacg 
gggaatgcc tttttgcca tatatcgag aaaagtttc 
gaggaaatc ttcaaattg ataaattat ccacaggat 
gacatacgc aaggcttcg gtcgaagcc ctcctccag 
ttttgcata gctttacac aaaatcaac tcaaacgaa 
accaagcaa gctctctat aaagcttta caaatgttt 
gtaccaaaa tgtgccgaa cttatccga accgatgag 
gaacgaggc gttgtcatg tgctgtttg gacgcttac 
gcttccctg cttgaggaa gttaaaagt gacgttttg 
gtcggggaa ggccatcgg gaggctatc atgaattgt 
gtcattgac gtcctcacg ttaaaaaca atgtgtcaa 
gacacagat ttggacgca aatcccgaa aatacagac 
gaggaaaca gaggcggaa caagacgag ttgttgctt 
gaatcagcc ggtgatgtt ataccgaag ttcggagcg 
gcgataact cccgatgat tttgtttta tatttcccg 
aatattctc caactgctg acgcagaga actaaaaag 
cagcacagt gtgagccag cgttcgttc gcgttcggc 
actttggcc gaatgcatg aaatcgctg gacgtctac 
gtcgaaaaa ttcgttcag catttgctg catttgtgg 
ctgactggg gccaaagat tcggccgac gaggtgcga 
aataacgcg attttcggt cttggcgaa atgattttg 
cacgggaag gaccgaatt ttcgggtat ttccccgat 
atactccag gctttgtcc actgctgtg gctaaggag 
tcgcacgca gggactctg gataatatc tgcggtgct 
ttagctaag atgattatt gttaatcct gggggagtt 
cccctggat caggtgctc ccagcgttc ctccaacgc 
ctgcctctc agggacgat ttccaagag aacgaagct 
gtagttaag tgtttcttt actttgtat caacagggg 
aatcccatc ctgcgcgag catttatcc agtgttatc 
aaaattgtg gttcaggtg tacgacaag aaacaatat 
ccgaatgat gaaaccaaa aatatttta gtggaactg 
atcaaaacg ataaatagg gattttagt gaagagttt 
gggaacaca gtgtcttcc ttaggacca gaagtgact 
aaatcgtta cagaatttg tttgctact tcttaagac 
caaaacctt aattcctgt cctggcaat ctcagaagt 
ttttttatt ttttgggac atgtgttaa tgttttttg 
ttgagtcat atttttgtt tatttattg tatctgtgt 
atcgactat aataatgct gcatcttaa gagttttaa 
agtgattac tactgtttt taataaaaa attgcttcg 
gtt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY