Status
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Gene information
General information
TC030649 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 9972708..9975808
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030649
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02609

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030649 RA
      MSSEKESVS SPMSIEKYS LVLVDVAGT TTSIDFVKD 
      TLFPFVVKQ AEPFLQEKW EEESIKDCI KLIKGDADL 
      DLAAAVERV KALTQEDSS NKGLKTLQG LIYKDGYEK 
      GELKAHVFD DVPEAFETW AKNRRVAIY STGSVDSQK 
      LLFSNTVKG DLSAHISKY FDQSVGPKT EAESYKKIA 
      TETEAKPEE IVFITDDPK EATAAKSGG LAAVLIIRE 
      GNSPLPEEI SKEFTTISS FKDFDKTLK RKIEEAPTS 
      EEAPPSKLP KTDVEEKPT EEKAESMEV DAGKPEATE 
      TAQNETEQP TEKTENKEE TKEVTESEE KMETVVEET 
      KAEVATEEV NATTEQEKP TETEEPKEV EAAKTEEPK 
      ADDAKPEDP KPEEEKPTE EAQVPEEKP AEEAPEKTE 
      TEEVKVDTT DAPVENGEE KTEAAEKTE EKENKSVLA 
      NGVENGKEE SETVTDAAE SAEVKVDEV EKSDEAEVK 
      VKKLEESAE NITSKRMYC RVIVSFLVC TIWEISIFK 
      CQNFYWT
    CDS sequence
    • >TC030649 RA
      atgagttct gaaaaagaa agtgtttct tctccgatg 
      agcatcgaa aagtactct cttgtgctt gtggacgtt 
      gccggcaca acgacttcc atcgacttt gtaaaggac 
      actctcttt ccttttgtc gtcaaacaa gctgaaccc 
      tttcttcaa gaaaaatgg gaagaagaa tctattaag 
      gattgcatc aaattaatc aaaggagat gccgatttg 
      gatcttgca gccgcagtg gaacgtgtc aaagccttg 
      acccaagaa gatagctcc aataaaggt ctgaagacg 
      ctccaaggg ctgatctac aaagatggt tacgaaaag 
      ggcgaactc aaagcacac gtttttgat gatgttcct 
      gaagctttt gaaacttgg gccaaaaat cgccgcgtt 
      gctatctat tccactgga agcgttgat tcacaaaag 
      ttgcttttt agtaacacg gttaaaggc gatctttct 
      gcacacatt tccaaatat ttcgaccaa tctgtggga 
      cctaagact gaagctgaa agttacaag aagatagcg 
      acagaaact gaagccaaa ccagaagaa atcgtcttt 
      attactgac gaccctaaa gaagcaaca gctgctaaa 
      tctggagga cttgcggct gtcctaatt atcagagaa 
      ggcaattct cctttacct gaagagatc tcaaaagaa 
      tttacaact attagttcc tttaaagac tttgataaa 
      actctcaaa cgaaaaatt gaagaggca cccacttca 
      gaggaggcg ccaccttca aaactccct aaaaccgac 
      gtggaagaa aaacccact gaagagaaa gcagagtca 
      atggaagtt gatgctggt aagcctgaa gccactgaa 
      actgcccaa aacgagact gaacaacca acggaaaag 
      actgaaaat aaagaagaa accaaggaa gtcactgaa 
      tctgaggaa aaaatggaa acagttgtt gaagaaaca 
      aaagcagaa gtagcaact gaagaggtt aacgcaaca 
      acggagcaa gaaaaacca acagaaact gaagaacca 
      aaagaggtg gaggcagca aaaactgaa gaaccaaaa 
      gcagacgat gcaaaacct gaagatcct aaacccgaa 
      gaagagaaa cctacagaa gaggcacaa gtgcctgag 
      gaaaaaccc gctgaagaa gcccccgaa aagactgaa 
      acggaagag gttaaggta gacacaacc gacgcgcct 
      gtggagaac ggtgaagaa aaaaccgaa gctgcggag 
      aaaactgag gaaaaagaa aataagagt gtgctagcc 
      aacggtgtt gagaacgga aaggaagag agtgaaact 
      gtcacagat gcggctgag agcgctgaa gtcaaagtc 
      gatgaggtt gaaaagagc gatgaagcc gaggtcaaa 
      gtgaaaaaa ctagaggaa agtgctgaa aatatcaca 
      tctaaacga atgtattgt cgagtaatc gtaagtttc 
      ttggtttgt accatttgg gaaatttca attttcaag 
      tgccaaaat ttttattgg acttaa
    mRNA sequence
    • >TC030649 RA
      agtttatgt cgtaacaac tttcttttg atattttgt 
      agtgcgcaa gcgtaaacg atgcaaaat tcaccattt 
      tctccacct ccaccattc gtgttttgc atcgtccgt 
      tgttgccgt taatcacga aatttttta aactaaaaa 
      ccacaaatt ctgtgaaaa aacgttacc actaatcga 
      aacccatta ccgtaattg aatttaaga tgagttctg 
      aaaaagaaa gtgtttctt ctccgatga gcatcgaaa 
      agtactctc ttgtgcttg tggacgttg ccggcacaa 
      cgacttcca tcgactttg taaaggaca ctctctttc 
      cttttgtcg tcaaacaag ctgaaccct ttcttcaag 
      aaaaatggg aagaagaat ctattaagg attgcatca 
      aattaatca aaggagatg ccgatttgg atcttgcag 
      ccgcagtgg aacgtgtca aagccttga cccaagaag 
      atagctcca ataaaggtc tgaagacgc tccaagggc 
      tgatctaca aagatggtt acgaaaagg gcgaactca 
      aagcacacg tttttgatg atgttcctg aagcttttg 
      aaacttggg ccaaaaatc gccgcgttg ctatctatt 
      ccactggaa gcgttgatt cacaaaagt tgcttttta 
      gtaacacgg ttaaaggcg atctttctg cacacattt 
      ccaaatatt tcgaccaat ctgtgggac ctaagactg 
      aagctgaaa gttacaaga agatagcga cagaaactg 
      aagccaaac cagaagaaa tcgtcttta ttactgacg 
      accctaaag aagcaacag ctgctaaat ctggaggac 
      ttgcggctg tcctaatta tcagagaag gcaattctc 
      ctttacctg aagagatct caaaagaat ttacaacta 
      ttagttcct ttaaagact ttgataaaa ctctcaaac 
      gaaaaattg aagaggcac ccacttcag aggaggcgc 
      caccttcaa aactcccta aaaccgacg tggaagaaa 
      aacccactg aagagaaag cagagtcaa tggaagttg 
      atgctggta agcctgaag ccactgaaa ctgcccaaa 
      acgagactg aacaaccaa cggaaaaga ctgaaaata 
      aagaagaaa ccaaggaag tcactgaat ctgaggaaa 
      aaatggaaa cagttgttg aagaaacaa aagcagaag 
      tagcaactg aagaggtta acgcaacaa cggagcaag 
      aaaaaccaa cagaaactg aagaaccaa aagaggtgg 
      aggcagcaa aaactgaag aaccaaaag cagacgatg 
      caaaacctg aagatccta aacccgaag aagagaaac 
      ctacagaag aggcacaag tgcctgagg aaaaacccg 
      ctgaagaag cccccgaaa agactgaaa cggaagagg 
      ttaaggtag acacaaccg acgcgcctg tggagaacg 
      gtgaagaaa aaaccgaag ctgcggaga aaactgagg 
      aaaaagaaa ataagagtg tgctagcca acggtgttg 
      agaacggaa aggaagaga gtgaaactg tcacagatg 
      cggctgaga gcgctgaag tcaaagtcg atgaggttg 
      aaaagagcg atgaagccg aggtcaaag tgaaaaaac 
      tagaggaaa gtgctgaaa atatcacat ctaaacgaa 
      tgtattgtc gagtaatcg taagtttct tggtttgta 
      ccatttggg aaatttcaa ttttcaagt gccaaaatt 
      tttattgga cttaagact ggacccaaa gactgtttt 
      taatctcac ttttgtgat actcatttt ttaagacta 
      ctgaacagt attaaattt gtgtcactc attgtttag 
      gtgttttgt ccttatttt agctatgta actcgcttt 
      cttaatcac attcctaat gagtatact ggtacggtt 
      atttttgat attaacgca tttacttaa tttagtttt 
      gtaaaataa ctggcttcc agtgtgttc cacttttgt 
      tccattttt atggctcat gagattgtg atattaaaa 
      aaaattgaa aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY