Status
Please wait ...
Gene information
General information
TC033813
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030637,TC030722
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 3256841..3264652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033813
    iBeetle screen iB_02605
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not closed - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033813 RA
      MENGRPGIS AILFDLDNT LIATRKADK QTCSQISDA 
      VKKCINILK NTNSDTEKF AALFMVTKL AKGKDCNAV 
      AKKAIFEAI GFDFIKRLL LTDDVPVDC PPNIYKSVA 
      LSILTVFCN EEELATHPE MLVNVPVFL DIVKKADDE 
      DLMSVGETY NCLKGIASY PPGQQALIE AGGIAKLSE 
      IYSQGSFQS DEALNILAV LVGNLGIKS WDEDNPQTF 
      HNLLNKIAV DFETDHDKR KFELCQVLN ALIFNCPKK 
      IVNSADKET WPMSLYKGL TDILQSKIG PQQRNPALI 
      LASSVLDAL GVEWAMSDE EKPKQFFLL LIQLAAIEV 
      RMQLDGKSL KAATAQRNL VTACYIILE LSINYISTD 
      QLDLDQKEK QTLYTGLKG AFTAIINLL LKVAADKNK 
      PSGDEKWFV YATIRVLAA WLAQETSAL RIQVYQLLP 
      FILEIANES FNAHKAKRV AEKAGIESE TNPANSVDI 
      LRILLPGLC HLAVEDAAR AIILKHHED KVLFDLVQF 
      HWSIVHYKR PPVPRSERL KVLNQPKQE LSPELLAEM 
      QDSRTAMIS ACNVLMNLT VLETKYVEE SDVFNDLLK 
      FIFENLPEL KDIPENLVL HGNLAVLGL LLLKQLSNK 
      VKKNDFSIC RYIQATIRF LWDAYIIDE SNDPTALVV 
      SMAYKEHWM ELMELWFLG MQTMSGVIA QIPWISEFA 
      IESGWAEGI VQTLKKVKI GTLPPNVKS AYEDFLCHL 
      VDANESVTD VLKKAEALR LAQILWEKW DVPTDFATN 
      ASKAFLKAF RKCPENLSM SLDAWRRLL WAQALGDQY 
      NKYAGEVYR MWLQLRYDN LALSPEIQN LLIKLRQHY 
      FVGLITNGT SRAQWEKIQ LLHLQSFFD VVLVSGDLP 
      WEKPHREIF NIACEYLGV EPQQCIMVG DKLETDILG 
      GLKAKLGGT VWVPLNSIE VGDEDPRPD YVIKNVTEL 
      PNLLPKNPK VPRFRGREE LRTRQISAP DFEDSNSNS 
      SDGS
    CDS sequence
    • >TC033813 RA
      atggaaaat ggacgcccc ggaatatcc gccatcctc 
      ttcgacctg gacaacacg ctcatcgcc acccggaag 
      gccgacaag cagacgtgc agccagatc tccgatgct 
      gttaaaaag tgcatcaat attctcaag aacaccaac 
      agcgatacg gagaagttc gccgctttg tttatggtc 
      accaagttg gccaaaggt aaagactgc aatgccgtg 
      gcgaagaaa gccatcttc gaagcgatt ggtttcgat 
      ttcatcaaa cgtcttctt ctcaccgat gacgttccc 
      gtcgattgc ccaccgaac atctacaaa tcggtggct 
      ctttcaatc ctcaccgtt ttttgcaac gaggaggag 
      ttagccact caccctgaa atgttagtt aacgtcccc 
      gttttcctc gacattgtt aaaaaagcc gacgacgag 
      gatctcatg tcggttgga gagacatac aactgtctc 
      aagggcatc gctagttac ccccctggg caacaggcc 
      cttatcgaa gccggaggt atcgcaaag ctgagcgag 
      atttactcc caagggagt ttccagtca gacgaggca 
      ttgaacatt ttggccgtt ttagtcggc aatttggga 
      ataaaaagc tgggacgag gataatcca caaactttt 
      cataattta cttaataaa atcgcagtc gatttcgaa 
      acagaccac gataaacga aaattcgag ttatgccaa 
      gtgcttaac gccttgatt tttaattgt ccgaagaag 
      attgtgaac agtgcagat aaggaaacc tggcctatg 
      agtctctat aagggcctc actgatata ctgcagagt 
      aaaattgga ccacagcaa cgcaatccg gctttaatt 
      ttggcatcg agtgttttg gatgctttg ggagtcgag 
      tgggctatg tccgatgag gagaaacct aaacagttt 
      ttcttactc ttgatacag ttggcagca attgaggtc 
      cgaatgcaa cttgatggc aaaagtctc aaagctgca 
      accgcccaa aggaatctt gtaacggct tgttacatt 
      attctagaa ttgtcaatt aattatatt tcgacggat 
      cagttggac ttggatcag aaggagaag cagacactt 
      tataccggt ttgaaggga gcattcact gctattata 
      aacctactg ttaaaagtg gccgctgat aagaataaa 
      ccttctggc gatgaaaaa tggtttgtt tatgctacc 
      atcagagtt ctggcagct tggttggct caagaaacc 
      tccgcgtta cgcatccag gtctaccaa ctcctaccg 
      ttcattctc gaaatcgcc aatgaaagt ttcaatgcg 
      cataaggcg aaaagagtt gccgagaaa gcagggatt 
      gaaagtgaa acaaaccct gccaattct gtggatatt 
      ttgcgaatt cttctgcca ggtctttgc catttggcc 
      gtggaggat gcagcacgt gccattatc ttgaaacac 
      catgaagat aaagttctc ttcgacttg gtgcagttt 
      cactggagc atcgttcat tataaacgt ccaccggtt 
      ccacgatcc gaacgtctg aaagttcta aatcaacca 
      aaacaggaa ctatcgccc gaacttttg gccgaaatg 
      caagactcc aggactgcc atgatcagc gcctgtaac 
      gtattgatg aatctgact gtgctggaa acgaaatac 
      gtcgaagag agtgatgtt ttcaacgat ttgcttaaa 
      tttattttt gagaatttg ccggagttg aaggatatt 
      cccgagaat ctcgttctt cacgggaat ttggccgtg 
      ttggggctt ttgttgctg aagcagctc tcgaataaa 
      gtgaagaag aatgatttt tcgatctgt cgttacatc 
      caggcgact attaggttt ttatgggat gcgtacatc 
      attgatgag tcaaatgac ccaaccgct ttggttgtg 
      tcaatggcg tacaaagaa cactggatg gagcttatg 
      gaactctgg tttttggga atgcaaacc atgtcaggc 
      gtcatcgcc caaatcccc tggatttct gaatttgcg 
      attgaaagc ggttgggcc gaaggcatc gtccagaca 
      ttgaagaaa gtcaaaatc gggactctc ccaccaaac 
      gttaaatca gcttatgaa gatttcctc tgccactta 
      gtcgacgcc aacgagagt gttaccgat gttttgaag 
      aaagctgaa gctttgagg ctagcgcaa attctgtgg 
      gagaaatgg gacgttcca actgatttt gcaacgaat 
      gcctcgaaa gcctttttg aaggctttc aggaaatgc 
      cccgagaat ttatcgatg agtttggat gcgtggagg 
      aggctgctt tgggctcag gcgctgggg gatcagtac 
      aacaagtat gcaggggaa gtgtatcgc atgtggcta 
      cagttgcgt tatgacaac ctggctctg tctcctgaa 
      atacaaaac ttgctaata aagctgcgc cagcactac 
      tttgtcggc ttgataaca aacggcacg tcgagggcc 
      caatgggag aagatccag ctgttgcat cttcagagt 
      tttttcgat gtcgtgcta gtgtcgggg gacctcccg 
      tgggagaag ccccacagg gagatcttc aacatagct 
      tgcgagtat ctgggggtc gagccgcag caatgcatc 
      atggtgggc gataagtta gagaccgac atcttgggg 
      ggcttgaag gcgaaattg ggagggaca gtgtgggtg 
      cccttgaat tcgatcgaa gttggggat gaagatccc 
      aggcccgat tatgttatc aaaaacgtg accgaatta 
      ccaaatctt ttaccgaaa aatcccaaa gtacctagg 
      tttaggggg agagaagaa ttgcgcact cgtcaaatc 
      agcgcacct gatttcgaa gacagcaac agcaacagc 
      agcgatggc agctag
    mRNA sequence
    • >TC033813 RA
      agtcacgtg actcgcgca acgtgacag tatccgatg 
      acctcatcg gagcggatc cattgtagt agagggggg 
      cctgggagg tcgtgaggt ccattttct gtctttcca 
      tgacatatt gcgggggaa agtttatcc ggagtgcat 
      tttaaaccg ttgaggctc ttctcatgc cccgctggc 
      actccgagc catggaaaa tggacgccc cggaatatc 
      cgccatcct cttcgacct ggacaacac gctcatcgc 
      cacccggaa ggccgacaa gcagacgtg cagccagat 
      ctccgatgc tgttaaaaa gtgcatcaa tattctcaa 
      gaacaccaa cagcgatac ggagaagtt cgccgcttt 
      gtttatggt caccaagtt ggccaaagg taaagactg 
      caatgccgt ggcgaagaa agccatctt cgaagcgat 
      tggtttcga tttcatcaa acgtcttct tctcaccga 
      tgacgttcc cgtcgattg cccaccgaa catctacaa 
      atcggtggc tctttcaat cctcaccgt tttttgcaa 
      cgaggagga gttagccac tcaccctga aatgttagt 
      taacgtccc cgttttcct cgacattgt taaaaaagc 
      cgacgacga ggatctcat gtcggttgg agagacata 
      caactgtct caagggcat cgctagtta cccccctgg 
      gcaacaggc ccttatcga agccggagg tatcgcaaa 
      gctgagcga gatttactc ccaagggag tttccagtc 
      agacgaggc attgaacat tttggccgt tttagtcgg 
      caatttggg aataaaaag ctgggacga ggataatcc 
      acaaacttt tcataattt acttaataa aatcgcagt 
      cgatttcga aacagacca cgataaacg aaaattcga 
      gttatgcca agtgcttaa cgccttgat ttttaattg 
      tccgaagaa gattgtgaa cagtgcaga taaggaaac 
      ctggcctat gagtctcta taagggcct cactgatat 
      actgcagag taaaattgg accacagca acgcaatcc 
      ggctttaat tttggcatc gagtgtttt ggatgcttt 
      gggagtcga gtgggctat gtccgatga ggagaaacc 
      taaacagtt tttcttact cttgataca gttggcagc 
      aattgaggt ccgaatgca acttgatgg caaaagtct 
      caaagctgc aaccgccca aaggaatct tgtaacggc 
      ttgttacat tattctaga attgtcaat taattatat 
      ttcgacgga tcagttgga cttggatca gaaggagaa 
      gcagacact ttataccgg tttgaaggg agcattcac 
      tgctattat aaacctact gttaaaagt ggccgctga 
      taagaataa accttctgg cgatgaaaa atggtttgt 
      ttatgctac catcagagt tctggcagc ttggttggc 
      tcaagaaac ctccgcgtt acgcatcca ggtctacca 
      actcctacc gttcattct cgaaatcgc caatgaaag 
      tttcaatgc gcataaggc gaaaagagt tgccgagaa 
      agcagggat tgaaagtga aacaaaccc tgccaattc 
      tgtggatat tttgcgaat tcttctgcc aggtctttg 
      ccatttggc cgtggagga tgcagcacg tgccattat 
      cttgaaaca ccatgaaga taaagttct cttcgactt 
      ggtgcagtt tcactggag catcgttca ttataaacg 
      tccaccggt tccacgatc cgaacgtct gaaagttct 
      aaatcaacc aaaacagga actatcgcc cgaactttt 
      ggccgaaat gcaagactc caggactgc catgatcag 
      cgcctgtaa cgtattgat gaatctgac tgtgctgga 
      aacgaaata cgtcgaaga gagtgatgt tttcaacga 
      tttgcttaa atttatttt tgagaattt gccggagtt 
      gaaggatat tcccgagaa tctcgttct tcacgggaa 
      tttggccgt gttggggct tttgttgct gaagcagct 
      ctcgaataa agtgaagaa gaatgattt ttcgatctg 
      tcgttacat ccaggcgac tattaggtt tttatggga 
      tgcgtacat cattgatga gtcaaatga cccaaccgc 
      tttggttgt gtcaatggc gtacaaaga acactggat 
      ggagcttat ggaactctg gtttttggg aatgcaaac 
      catgtcagg cgtcatcgc ccaaatccc ctggatttc 
      tgaatttgc gattgaaag cggttgggc cgaaggcat 
      cgtccagac attgaagaa agtcaaaat cgggactct 
      cccaccaaa cgttaaatc agcttatga agatttcct 
      ctgccactt agtcgacgc caacgagag tgttaccga 
      tgttttgaa gaaagctga agctttgag gctagcgca 
      aattctgtg ggagaaatg ggacgttcc aactgattt 
      tgcaacgaa tgcctcgaa agccttttt gaaggcttt 
      caggaaatg ccccgagaa tttatcgat gagtttgga 
      tgcgtggag gaggctgct ttgggctca ggcgctggg 
      ggatcagta caacaagta tgcagggga agtgtatcg 
      catgtggct acagttgcg ttatgacaa cctggctct 
      gtctcctga aatacaaaa cttgctaat aaagctgcg 
      ccagcacta ctttgtcgg cttgataac aaacggcac 
      gtcgagggc ccaatggga gaagatcca gctgttgca 
      tcttcagag ttttttcga tgtcgtgct agtgtcggg 
      ggacctccc gtgggagaa gccccacag ggagatctt 
      caacatagc ttgcgagta tctgggggt cgagccgca 
      gcaatgcat catggtggg cgataagtt agagaccga 
      catcttggg gggcttgaa ggcgaaatt gggagggac 
      agtgtgggt gcccttgaa ttcgatcga agttgggga 
      tgaagatcc caggcccga ttatgttat caaaaacgt 
      gaccgaatt accaaatct tttaccgaa aaatcccaa 
      agtacctag gtttagggg gagagaaga attgcgcac 
      tcgtcaaat cagcgcacc tgatttcga agacagcaa 
      cagcaacag cagcgatgg cagctagaa ggggcccat 
      gatgagtca gtacatgat tgcagtatt ttttcaatt 
      aggtttcta caccaaagt ttaggtaat gagtcacgt 
      tacttaagg tacaaaatc tcgcagttg tgatagata 
      ttctaaatt tgctgtgaa tttgtggct gactagtta 
      gaagtaggt tacgttctc taggaatat aggtacgtt 
      agaggttat attttctta aaaaatgtt gaaaaagtg 
      tggcacaaa gatagatac gggtaccat gaatttttc 
      gttttttta aattttaag aatgtatcg ttgtgttca 
      gtactgtat tttttaaag aatctggat aatacgtta 
      aagtggtcg aaacaacgc attgttata ttgttgtta 
      gatatttaa ggagacaaa taatttaag ttataaaga 
      gaagatttt aaaaataat tgtgcaata tggactttg 
      tccgatttg tgcaaaaat cagaaacag aagtcatct 
      gatggtaat gaagtgcaa ataattatt ttttgtgta 
      aggatttat ttaagttgg tgtgggata taatagtat 
      aaagagaaa taactgctc tactaaatt tttgttaat 
      ttttaagtt tgtttcagt aagatctga gctatagta 
      atgtactat atcgactta tcagagaaa tcagtcatt 
      tagctagga ttcctatgt acagattcc actttaatg 
      tagtgtaac aacgtaata ttcaaatgt aacaattag 
      acgcccaac ttacttaac tcgactgta tcattatca 
      tcacataac aattaccta tgcataaac gatatattt 
      tcatgtact tttatacat ttaatgata acagtatcc 
      tatgtaaca tacgtctta ataaatttc ttagattca 
      t