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Gene information
General information
TC030636 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10413419..10415915
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030636
    iBeetle screen iB_02604

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030636 RA
      MGVNNSKKL AAKCSFLSK DEQHIVSNS FRLASKNSE 
      KIKEEELTK LWGTQMDSR LLQYINNYL FGIGESRTQ 
      TVELERFAE LFVFCTRGT VEEKLKVLI ISLGRSDSE 
      FNELPYILV KEYVESIVA SYMKIQKLS NTKQFKSWF 
      SRGCFTSAQ NIQRLAESL SHDLAAADS ITRRALEVW 
      LQGSTVLGQ LLLFVFMHL YNISHKDKA QVSGEKSAV 
      EEQHTSSEK EKDRSLVPF CRGLDLIPS YPSLLDLNQ 
      IVFINANLP QQYQLEWRF LFSSEIHGE SFSTLIGRI 
      VNQGPSVLV VEDRNGYMF GGFAPANWS LGPNFFGDD 
      SSFLFTLAP RMRIFPSTG YNQHFQYLN LHQQTMPNG 
      LAMGGQHNY YGLWIDSEY GKGHSSESC TTYSGYSQM 
      ASGKDFTFR HLEVWGLGA PPPTPQEKG ERSSSTSVL 
      DGNAETKAM LKMAGRTIH SEGLRDAPP NN
    CDS sequence
    • >TC030636 RA
      atgggcgtg aacaacagt aagaaattg gccgccaag 
      tgttccttt ttgtccaaa gatgaacaa catattgtt 
      agcaactcg ttccggctc gcaagcaag aactcggag 
      aaaatcaaa gaagaggaa cttacgaaa ttatggggc 
      acgcaaatg gactcccgc cttctgcag tacataaac 
      aattactta tttggcatt ggggaatca cgcacacaa 
      acggttgag ctggagcgg tttgccgaa cttttcgtc 
      ttttgcaca aggggcacg gttgaggag aagctgaaa 
      gttcttata atatcgctt ggacgcagc gattctgaa 
      tttaatgag cttccatac attcttgtc aaagaatac 
      gtggaaagt atcgtcgcg tcgtacatg aaaatccag 
      aagttgagt aacacaaaa cagttcaaa tcatggttt 
      tctcgcggc tgtttcacc tcagcccaa aacatccag 
      agactggca gagagcctc tctcacgat ttggcggcg 
      gctgatagc ataacgcga cgtgctcta gaagtgtgg 
      ttacagggt tcaacagtg cttggtcaa ttgctcctc 
      ttcgttttt atgcattta tataacatt tctcacaaa 
      gataaagct caagtttcc ggcgagaaa agcgcagtt 
      gaggaacag cacacctcg agtgaaaag gagaaagac 
      aggagttta gtgccgttt tgccggggc ctcgatctc 
      atcccttcg tatccaagt ttgctagac ttaaaccag 
      atagttttt attaacgcg aatctgccg caacagtat 
      caattagag tggagattt ttgtttagt tccgaaata 
      cacggcgag tctttttct acgctcata ggtaggata 
      gttaatcag ggaccgtca gttctggta gttgaagac 
      agaaacggg tacatgttt ggcggcttc gcccctgct 
      aactggtcc ctgggcccc aattttttc ggggatgac 
      agcagcttt cttttcacg ctcgcaccg cgcatgaga 
      atttttcca tctacaggg tacaatcag cacttccag 
      tatctcaat ttgcatcaa caaaccatg ccgaacggt 
      ttggccatg ggggggcag cacaactac tacggtctg 
      tggatagac agcgagtac ggcaaaggc cactcttct 
      gaatcctgc accacgtat tcgggctat tcgcagatg 
      gcctctggg aaggacttc acctttcgg cacttggag 
      gtctggggg ctgggggcc ccgccgccc acgccgcag 
      gaaaagggc gagcgttca agctctact agcgttctg 
      gacggcaac gctgagact aaagccatg ttgaagatg 
      gccggacgc acaatacat agtgaaggt ttaagggat 
      gcacctccg aataattaa
    mRNA sequence
    • >TC030636 RA
      aaccaccga gcgagatag aagatggac gacagttgg 
      ccgaaacgt caaaccacc tgagtttac cttcatatt 
      gcaaatttt ccacaagtt ggtctgtaa acagaaatt 
      agtggttgt ttaaaggtg attctcgtc tcgccgatc 
      atgcggaga gtgcctgat tcagcaatg aaaagtcac 
      tggttaacg cagcgtttt aaataattg gaaacttgt 
      gatacctga ccaaggctt tccgagggg agatttcgc 
      agtaattgt gatgggcgt gaacaacag taagaaatt 
      ggccgccaa gtgttcctt tttgtccaa agatgaaca 
      acatattgt tagcaactc gttccggct cgcaagcaa 
      gaactcgga gaaaatcaa agaagagga acttacgaa 
      attatgggg cacgcaaat ggactcccg ccttctgca 
      gtacataaa caattactt atttggcat tggggaatc 
      acgcacaca aacggttga gctggagcg gtttgccga 
      acttttcgt cttttgcac aaggggcac ggttgagga 
      gaagctgaa agttcttat aatatcgct tggacgcag 
      cgattctga atttaatga gcttccata cattcttgt 
      caaagaata cgtggaaag tatcgtcgc gtcgtacat 
      gaaaatcca gaagttgag taacacaaa acagttcaa 
      atcatggtt ttctcgcgg ctgtttcac ctcagccca 
      aaacatcca gagactggc agagagcct ctctcacga 
      tttggcggc ggctgatag cataacgcg acgtgctct 
      agaagtgtg gttacaggg ttcaacagt gcttggtca 
      attgctcct cttcgtttt tatgcattt atataacat 
      ttctcacaa agataaagc tcaagtttc cggcgagaa 
      aagcgcagt tgaggaaca gcacacctc gagtgaaaa 
      ggagaaaga caggagttt agtgccgtt ttgccgggg 
      cctcgatct catcccttc gtatccaag tttgctaga 
      cttaaacca gatagtttt tattaacgc gaatctgcc 
      gcaacagta tcaattaga gtggagatt tttgtttag 
      ttccgaaat acacggcga gtctttttc tacgctcat 
      aggtaggat agttaatca gggaccgtc agttctggt 
      agttgaaga cagaaacgg gtacatgtt tggcggctt 
      cgcccctgc taactggtc cctgggccc caatttttt 
      cggggatga cagcagctt tcttttcac gctcgcacc 
      gcgcatgag aatttttcc atctacagg gtacaatca 
      gcacttcca gtatctcaa tttgcatca acaaaccat 
      gccgaacgg tttggccat gggggggca gcacaacta 
      ctacggtct gtggataga cagcgagta cggcaaagg 
      ccactcttc tgaatcctg caccacgta ttcgggcta 
      ttcgcagat ggcctctgg gaaggactt cacctttcg 
      gcacttgga ggtctgggg gctgggggc cccgccgcc 
      cacgccgca ggaaaaggg cgagcgttc aagctctac 
      tagcgttct ggacggcaa cgctgagac taaagccat 
      gttgaagat ggccggacg cacaataca tagtgaagg 
      tttaaggga tgcacctcc gaataatta aacaaaggt 
      tggttttat tttgctgaa atgccttaa acggttctt 
      ttttaatct ttacactaa gatgacaag tgttttaat 
      cgatcccat gaagtattt ttatatgtg ccgtaattc 
      actatgtga gaatttcgc ttcgtatta caccaattg 
      ctatcacgt tacgttaag tctgggttg tcaatagca 
      ccgtaatgc gtacttgtg tgataacat tttaacaat 
      tttcctatt tttttaatt ataagaaaa gacgacgtg 
      ccaaagacc tggtcacac acttgtagt gagcttcac 
      tatttattt attttcccc gttttgatt gttacgact 
      atagatagt ttatatatt tgttattta tgtgtctta 
      catgtgatc tatcaattt ttaagtcat gtacagtaa 
      caatttgtc aaattaaac aagatccaa gtac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY