Status
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Gene information
General information
TC030632 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 7177267..7204258
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030632
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02603

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber mostly not present - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030632 RA
      MSVCLETSS VLANQVSTS TSPPITEEA PIAPQSAPE 
      QTESAPPQP TPVRTIAKL PTANAALSK ASLITVLNT 
      PLGNVKTLC VTPAVSSNA TGQFTVVNS ATPLNVANS 
      TGKPQTITL INTPVTVVK TISPSVEKS APEVVPNSS 
      NVVATAAAT VPALIENRG HNVFVKNTA CADNVTVAQ 
      DVPQSHKLL TANNFPTNN VLTTAINVS DVQKTANGQ 
      RNKVKILSN VLMPGTSQG NVLLNKPLN VPQRYAPRP 
      KLLGANNGA TTKYLNKPP NNMVNKQPV NMINKQQPQ 
      RVPYPTHKS QIKTLPPVN NYLHGKQPG IKTLPPHKG 
      VPGQIQRTG SGLRTIPPQ RPQKIANKP NYIGKHAVQ 
      AQKLKQPHK LKSMKANQL YNNYHGPPV QEKQMTFTQ 
      ALTAEIIET LSNKTGNMT GSYSNKSYE SLPPRYENS 
      YYSPEMKPT FSTDQARPM EERTKSGLD LIYQAVLLD 
      HNYNATLPP ESPNRSIPT PNTPSQMNG VSGSSMYSP 
      GSGKRRTLS ASNPISSST SLSTLSAVA SNSNSVIGL 
      QDDDAASDI SDGSDRKQD TEGEETDTA PEAEAVNNE 
      DQFGDYVTR CICGFIHDD GYMIECDRC KVWQHVQCV 
      VKNKQVPEE YLCEVCDPH KHIDRQKAR MVQQQWLRE 
      RQFADPKLR KDTKLKEVF KHKESLSDS DTSDGEHTG 
      HNNNIVGKG RPTLVNRRK SDSHQKGAR QRRDSAKEV 
      VQRRQIKKR ERKVVKRKT KAQLKNHSD DENHDSVAS 
      THLPQLRQW IENYEEAVT NHYSPELRA RISNIRVNG 
      AHNDISIQF DPSVHKCRV HTQPLTELK FLVSTVHLP 
      PNSPVIELR GKYMLSAQH RNSGSTFNT RQHAQRPGP 
      FLFFYKLHK DNTEVCVDT RTYGNSARF IRRSCKPNA 
      ELRHSIVKG VLHLYIVTI ANVDKNVEL TIKHESHDL 
      AAIETTHIA CACGNPDEC TVNRTSIKK NGESTEMHK 
      KRRGRRTTS VSLSSESET PNKPKKETL PQPLASPPP 
      PPAVVKHEE DEEEDVKPE VVEQPVKTE PEDIKPEVK 
      EEVTEPVPE DVKDVKVKE EEVKPVTPS TPPTPPITT 
      RRSSSHHKA EKEECNKEP EVKEDSSPP TEKNKNKKL 
      SREERKLEA ILRAIAQME KADQRKQEH QAKQAHRRE 
      SEPGPGKDE DKAEPKMKR KRRKGRART SSTHTPASR 
      RNRLNSTDS YLTSGDETL LSPHDSAPP NRPSMKDTE 
      YKENTQGNQ QNTSDKDVG LLLALSNGE KTNKSPPRE 
      TDSNSNSAQ SSPETHLSS ACLLVQAAI FNVSNNIIK 
      RRSCFKES
    CDS sequence
    • >TC030632 RA
      atgagtgtg tgtttagaa accagctca gtgctcgcc 
      aatcaggtg agcacctcg acgtcgcct ccgatcaca 
      gaagaggcc ccaattgcg ccccaatca gcacccgaa 
      caaaccgaa tcagctcct ccgcaacca accccggtc 
      aggaccata gccaagctt ccgacagct aacgccgcc 
      ttgtcgaag gcgtcgtta ataaccgtg ttgaacact 
      cctttggga aatgttaaa acgttatgt gtcacacct 
      gcagtttcg tcaaatgcc actggtcag ttcactgtc 
      gttaattca gcgactccg ttaaacgtc gcaaattca 
      actggaaaa ccacaaacc atcactttg atcaatacg 
      ccagttacc gttgtgaaa acaatttca ccgagtgtt 
      gaaaaaagt gctcctgaa gtcgtacct aacagttca 
      aatgttgtt gccactgct gcagctacg gtgccagct 
      ctcatcgaa aatcgggga cataatgtg tttgttaaa 
      aatacggct tgtgcggac aatgtcaca gtggcacag 
      gacgttccg caaagtcac aaattactc accgccaat 
      aattttccc acaaataac gtcctcaca actgcgatt 
      aatgtgagc gatgttcaa aaaacggca aacggtcag 
      agaaacaaa gtaaagatt cttagcaat gttttaatg 
      ccaggcact tcgcaaggc aatgtcctc ctgaataag 
      cccttgaac gttccgcaa cgttacgcc ccgcgtccg 
      aaactactc ggggcaaat aacggtgct acgacaaaa 
      tacttgaac aaaccccct aataatatg gtaaacaag 
      caacctgtt aacatgata aataaacag caaccgcag 
      agagtccca tatccgact cacaaaagt caaataaag 
      acgcttcca cccgttaac aattattta catggaaaa 
      cagccaggc atcaaaact ttgccgcca cacaagggg 
      gttccgggg cagattcaa aggacgggt tcaggcctg 
      aggaccata ccgcctcaa aggccgcaa aaaatcgcc 
      aataaaccc aactacatc ggaaaacat gccgtacaa 
      gcacaaaaa ctgaaacaa cctcataaa ctaaaaagt 
      atgaaagct aatcaattg tataacaat taccacgga 
      ccgcccgta caagaaaaa caaatgacg tttactcaa 
      gctttaaca gcggagata atcgaaact ttgagcaac 
      aaaactggc aatatgacg ggttcgtat tcgaataaa 
      agctacgaa agcttacca ccacggtac gaaaactcg 
      tactattcg ccagaaatg aaaccaaca ttctcaacg 
      gatcaggcc agaccaatg gaggaacga accaaatcc 
      ggtctggac ctcatctac caagcggtg cttctcgat 
      cataattac aatgctact cttccacct gaatctccc 
      aacagatca atacccacg cccaacaca ccttcgcaa 
      atgaacggg gtttccggt agttcgatg tactcgccc 
      ggaagcggt aaaaggcgg acgttatca gcctccaat 
      ccgatttca tcgtctaca agtttgtcg acactgtcg 
      gcggttgcg agcaacagc aactcggtg attggcttg 
      caagacgac gacgccgcc tccgacatc agcgacggt 
      tcggaccgc aagcaggac accgagggc gaagagacg 
      gacaccgcc cccgaggcg gaagccgtc aacaacgag 
      gaccagttc ggtgattac gtcacacgg tgcatttgc 
      gggttcatc cacgacgac ggctacatg atcgaatgc 
      gaccgctgt aaagtgtgg caacacgtc cagtgcgtg 
      gttaagaat aaacaagtc cccgaggag tacctttgc 
      gaagtttgc gacccgcac aagcacatc gataggcag 
      aaggcgcgg atggtgcaa cagcagtgg ctgcgagag 
      agacagttt gccgatccg aagttgcgc aaagatacg 
      aaattgaag gaggtgttc aaacataag gagagtttg 
      agcgatagc gatacgtca gatggggag catacaggg 
      cataataat aatatcgtg gggaaagga cggccgact 
      ttggtcaac aggaggaag agtgacagc catcaaaag 
      ggagcgaga cagaggagg gattcggcg aaggaagtg 
      gtgcaaagg aggcagatt aagaagagg gagaggaaa 
      gtcgtcaag cgaaaaact aaggctcag ctgaaaaac 
      catagcgac gatgaaaac cacgactcc gtggcctcc 
      actcacttg ccacagctt cgccaatgg atcgaaaac 
      tacgaagaa gccgtcacc aatcactac tcgcccgag 
      ttacgcgcc cgcataagc aacattcgc gtgaatggt 
      gcccataac gatatttcg attcagttt gatccatcg 
      gttcataaa tgtcgcgtt catacacaa cctttgacc 
      gaattgaaa tttctcgtc agtaccgtt catctaccg 
      ccgaattca cccgtgatt gaattacgc ggcaaatac 
      atgttatcg gcacaacat cgcaattcg ggaagcacg 
      ttcaacacg cgacaacac gctcaacgt cccggtccg 
      ttcctcttt ttctacaag ttgcacaag gacaatact 
      gaagtgtgc gttgatacg aggacgtac ggaaatagc 
      gcacgattt attcgacgg tcgtgtaaa ccgaacgcc 
      gaactgaga cattcgatt gttaaaggt gttctgcat 
      ttgtacatt gtgacgatc gcgaatgtg gataaaaat 
      gtcgagttg acgattaaa cacgagtcg cacgatttg 
      gccgctatt gagacaacg catattgcg tgcgcgtgc 
      ggaaatccc gacgaatgt acagttaat aggacaagc 
      atcaagaaa aatggagag tcgaccgaa atgcacaag 
      aagagacgc ggccgtcgc actacttct gtttctctg 
      tcgtcggaa tccgaaact cccaacaaa ccgaagaaa 
      gaaaccctc ccccagccc ctcgcatcg ccgcctccg 
      cccccggca gtagtgaaa cacgaggag gatgaagaa 
      gaagacgtc aaaccggag gttgtcgaa cagccggtt 
      aagactgaa cctgaagat attaaaccc gaagtgaaa 
      gaagaagtc acggaaccg gtgcccgaa gacgtgaaa 
      gatgtcaaa gtgaaggaa gaagaggtg aaaccggtg 
      acaccgtca acgcctccg acaccgcca atcactaca 
      agacgttcg tcgtctcat cacaaggcc gaaaaggag 
      gaatgcaac aaggaaccg gaagtgaag gaggattcg 
      tcacctccg acagagaaa aacaaaaat aagaagttg 
      agtcgcgag gagcggaag ttggaagcg attttgagg 
      gccatcgct cagatggaa aaggccgac cagcgaaaa 
      caggagcat caggccaaa caggcgcat cggagggag 
      tccgagccg gggccgggc aaggacgag gacaaggca 
      gagccgaag atgaagcga aaaaggcga aaaggcaga 
      gcgcgaacg tccagtaca cacacgccg gccagtcgt 
      cgaaatcgt ctcaattcg accgattcg tacctcact 
      tcaggcgac gaaacattg ctttctccg cacgacagc 
      gctccaccg aatcgtcct tccatgaaa gataccgag 
      tacaaagag aatactcaa ggcaatcag caaaacacg 
      tccgacaaa gacgtcggt cttcttttg gcactttcg 
      aatggtgaa aaaacgaat aaatcgcca ccgcgtgaa 
      accgattcc aattcgaat tcggcacaa tcctcgcct 
      gaaacacac ctgtcgtcg gcttgttta ctggtacaa 
      gcggccatt ttcaacgtc agtaataat attattaag 
      aggcgctcc tgttttaaa gaaagttaa
    mRNA sequence
    • >TC030632 RA
      cgagattat caagtaggt ttcgtagct acatttcct 
      ttgttttgc attgaatat ggctgccat atttagttt 
      agatgtctg ttttcaaaa gcgtatcag aatttgtga 
      taaattcta cataaaatt atagcgaat taacgttgt 
      gtttgtcga ataagacgg tgggatgtg ttagaagtt 
      caatggacg tttaaaaaa ttgctgccg tgttgtttt 
      taagttaaa atgcttgtg tttacatcg actgtcctg 
      gacttgtgt acgatttat ggcaattta aggaattat 
      cagtgacct ggagtcgga agttttaga ggagggtgg 
      tggcgggga caccgcctt tgaaccggt accaaacca 
      tggacttac ttgtgattc ccaagaaga aaccatgag 
      tgtgtgttt agaaaccag ctcagtgct cgccaatca 
      ggtgagcac ctcgacgtc gcctccgat cacagaaga 
      ggccccaat tgcgcccca atcagcacc cgaacaaac 
      cgaatcagc tcctccgca accaacccc ggtcaggac 
      catagccaa gcttccgac agctaacgc cgccttgtc 
      gaaggcgtc gttaataac cgtgttgaa cactccttt 
      gggaaatgt taaaacgtt atgtgtcac acctgcagt 
      ttcgtcaaa tgccactgg tcagttcac tgtcgttaa 
      ttcagcgac tccgttaaa cgtcgcaaa ttcaactgg 
      aaaaccaca aaccatcac tttgatcaa tacgccagt 
      taccgttgt gaaaacaat ttcaccgag tgttgaaaa 
      aagtgctcc tgaagtcgt acctaacag ttcaaatgt 
      tgttgccac tgctgcagc tacggtgcc agctctcat 
      cgaaaatcg gggacataa tgtgtttgt taaaaatac 
      ggcttgtgc ggacaatgt cacagtggc acaggacgt 
      tccgcaaag tcacaaatt actcaccgc caataattt 
      tcccacaaa taacgtcct cacaactgc gattaatgt 
      gagcgatgt tcaaaaaac ggcaaacgg tcagagaaa 
      caaagtaaa gattcttag caatgtttt aatgccagg 
      cacttcgca aggcaatgt cctcctgaa taagccctt 
      gaacgttcc gcaacgtta cgccccgcg tccgaaact 
      actcggggc aaataacgg tgctacgac aaaatactt 
      gaacaaacc ccctaataa tatggtaaa caagcaacc 
      tgttaacat gataaataa acagcaacc gcagagagt 
      cccatatcc gactcacaa aagtcaaat aaagacgct 
      tccacccgt taacaatta tttacatgg aaaacagcc 
      aggcatcaa aactttgcc gccacacaa gggggttcc 
      ggggcagat tcaaaggac gggttcagg cctgaggac 
      cataccgcc tcaaaggcc gcaaaaaat cgccaataa 
      acccaacta catcggaaa acatgccgt acaagcaca 
      aaaactgaa acaacctca taaactaaa aagtatgaa 
      agctaatca attgtataa caattacca cggaccgcc 
      cgtacaaga aaaacaaat gacgtttac tcaagcttt 
      aacagcgga gataatcga aactttgag caacaaaac 
      tggcaatat gacgggttc gtattcgaa taaaagcta 
      cgaaagctt accaccacg gtacgaaaa ctcgtacta 
      ttcgccaga aatgaaacc aacattctc aacggatca 
      ggccagacc aatggagga acgaaccaa atccggtct 
      ggacctcat ctaccaagc ggtgcttct cgatcataa 
      ttacaatgc tactcttcc acctgaatc tcccaacag 
      atcaatacc cacgcccaa cacaccttc gcaaatgaa 
      cggggtttc cggtagttc gatgtactc gcccggaag 
      cggtaaaag gcggacgtt atcagcctc caatccgat 
      ttcatcgtc tacaagttt gtcgacact gtcggcggt 
      tgcgagcaa cagcaactc ggtgattgg cttgcaaga 
      cgacgacgc cgcctccga catcagcga cggttcgga 
      ccgcaagca ggacaccga gggcgaaga gacggacac 
      cgcccccga ggcggaagc cgtcaacaa cgaggacca 
      gttcggtga ttacgtcac acggtgcat ttgcgggtt 
      catccacga cgacggcta catgatcga atgcgaccg 
      ctgtaaagt gtggcaaca cgtccagtg cgtggttaa 
      gaataaaca agtccccga ggagtacct ttgcgaagt 
      ttgcgaccc gcacaagca catcgatag gcagaaggc 
      gcggatggt gcaacagca gtggctgcg agagagaca 
      gtttgccga tccgaagtt gcgcaaaga tacgaaatt 
      gaaggaggt gttcaaaca taaggagag tttgagcga 
      tagcgatac gtcagatgg ggagcatac agggcataa 
      taataatat cgtggggaa aggacggcc gactttggt 
      caacaggag gaagagtga cagccatca aaagggagc 
      gagacagag gagggattc ggcgaagga agtggtgca 
      aaggaggca gattaagaa gagggagag gaaagtcgt 
      caagcgaaa aactaaggc tcagctgaa aaaccatag 
      cgacgatga aaaccacga ctccgtggc ctccactca 
      cttgccaca gcttcgcca atggatcga aaactacga 
      agaagccgt caccaatca ctactcgcc cgagttacg 
      cgcccgcat aagcaacat tcgcgtgaa tggtgccca 
      taacgatat ttcgattca gtttgatcc atcggttca 
      taaatgtcg cgttcatac acaaccttt gaccgaatt 
      gaaatttct cgtcagtac cgttcatct accgccgaa 
      ttcacccgt gattgaatt acgcggcaa atacatgtt 
      atcggcaca acatcgcaa ttcgggaag cacgttcaa 
      cacgcgaca acacgctca acgtcccgg tccgttcct 
      ctttttcta caagttgca caaggacaa tactgaagt 
      gtgcgttga tacgaggac gtacggaaa tagcgcacg 
      atttattcg acggtcgtg taaaccgaa cgccgaact 
      gagacattc gattgttaa aggtgttct gcatttgta 
      cattgtgac gatcgcgaa tgtggataa aaatgtcga 
      gttgacgat taaacacga gtcgcacga tttggccgc 
      tattgagac aacgcatat tgcgtgcgc gtgcggaaa 
      tcccgacga atgtacagt taataggac aagcatcaa 
      gaaaaatgg agagtcgac cgaaatgca caagaagag 
      acgcggccg tcgcactac ttctgtttc tctgtcgtc 
      ggaatccga aactcccaa caaaccgaa gaaagaaac 
      cctccccca gcccctcgc atcgccgcc tccgccccc 
      ggcagtagt gaaacacga ggaggatga agaagaaga 
      cgtcaaacc ggaggttgt cgaacagcc ggttaagac 
      tgaacctga agatattaa acccgaagt gaaagaaga 
      agtcacgga accggtgcc cgaagacgt gaaagatgt 
      caaagtgaa ggaagaaga ggtgaaacc ggtgacacc 
      gtcaacgcc tccgacacc gccaatcac tacaagacg 
      ttcgtcgtc tcatcacaa ggccgaaaa ggaggaatg 
      caacaagga accggaagt gaaggagga ttcgtcacc 
      tccgacaga gaaaaacaa aaataagaa gttgagtcg 
      cgaggagcg gaagttgga agcgatttt gagggccat 
      cgctcagat ggaaaaggc cgaccagcg aaaacagga 
      gcatcaggc caaacaggc gcatcggag ggagtccga 
      gccggggcc gggcaagga cgaggacaa ggcagagcc 
      gaagatgaa gcgaaaaag gcgaaaagg cagagcgcg 
      aacgtccag tacacacac gccggccag tcgtcgaaa 
      tcgtctcaa ttcgaccga ttcgtacct cacttcagg 
      cgacgaaac attgctttc tccgcacga cagcgctcc 
      accgaatcg tccttccat gaaagatac cgagtacaa 
      agagaatac tcaaggcaa tcagcaaaa cacgtccga 
      caaagacgt cggtcttct tttggcact ttcgaatgg 
      tgaaaaaac gaataaatc gccaccgcg tgaaaccga 
      ttccaattc gaattcggc acaatcctc gcctgaaac 
      acacctgtc gtcggcttg tttactggt acaagcggc 
      cattttcaa cgtcagtaa taatattat taagaggcg 
      ctcctgttt taaagaaag ttaaaatct catgggttt 
      tgccgcaga atttacgaa gaagatttg tataaagtg 
      ttcccgatt cgtcagcta aaattaaca ttttatgag 
      gacttgtgc cactgaaac gtttaagaa acctgtttc 
      taatactta aatgtttac tattactgt gattttcat 
      taattaata aattaggta actgagctg cactctcaa 
      aaatatttt cttcttcaa actcactgc agtttactt 
      gttgcagtt ctttataca atgacacat cagagaact 
      gttgatttg caatgaatg ggccttcag aagtgttat 
      cattgattg atttttctg caccgctag ataatgata 
      gcgaagaca agtcaagtt tttcatttt cattgtttt 
      ttaaagcat taatatcca aattgagac cttgtatta 
      ttacattaa gttcatcta ttttttgtt gttgttctt 
      ggagagttc tatttagct cactttcca tttcaaatg 
      cctcttttt taaagtgct caaacacct gtttaatgt 
      ctaataatt ctttatctc tagtggcaa tagtagtat 
      ttaagttct caattatac cttctagat cgtcaattt 
      gatctttcg taacaatta aacagtcat cagctgttg 
      aatactcag tacaacaga taattttat tatcagcta 
      gaaattttt tatttttta ttcgggaca aacaccctt 
      tgtggatta atttgaaac atcgtgtac agatataat 
      taataatta tgcgatctg gagcaacaa gcaaaattt 
      gcaaactca ctagatgag gtatccatt ggatgaaat 
      atctgttta aatggagaa aaaactaac agaaatcac 
      agattaaaa accaactac aaaaactat tggttgtgc 
      cctagcaca gttctgtta ttgttaaca acacactaa 
      taattatta ttaaacttg
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