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Gene information
General information
TC030629 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 4243104..4244399
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030629
    iBeetle screen iB_02602
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030629 RA
      MSEAIVCVK DFEKHAYNV LPRNALDYY RSGAGAEET 
      LAHNRKAFS KYKIRPRCL RNVAKRDLS TTVLGEKVQ 
      IPVGISPTA MQRMAHPEG ECANARAAQ AMGTIFTLS 
      TIATSSIEE VAQAAPYGT KWFQLYIYN DRNVTRRLV 
      ERAEKAGFK ALVLTVDTP MFGLRLADI RNKFVLPPH 
      LKFANFAGD KATGINQTE SGSGLNNYV NRLFDQSLE 
      WKDIKWLQS FTKLPIVVK GVLTAEDAL IAADLGVQG 
      ILVSNHGAR QVDGTPASI EALPEIVRA VGDRVEVYM 
      DGGITDGTD IFKALALGA RMVFFGRPA LWGLAHSGE 
      EGVKKILNI LKTELDYTM AITGCATVR DIDHRMVVH 
      ESYYSKL
    CDS sequence
    • >TC030629 RA
      atgagcgaa gcaatagtt tgcgtcaaa gactttgaa 
      aaacatgct tacaacgtc ctgccccga aatgcgctc 
      gattactat cgaagtggg gccggagct gaagagact 
      ctggctcat aacaggaaa gctttctcc aagtacaaa 
      attcggccg cgatgttta agaaacgtg gccaaaaga 
      gacctctcg accacagtt ttgggcgag aaagtgcaa 
      atcccggtc gggatttcc cccacagcc atgcagagg 
      atggcgcac ccagagggc gagtgtgcc aatgcacga 
      gctgctcag gcaatgggc accatcttc actctaagc 
      accatcgca actagcagc atcgaagaa gtggcccaa 
      gcggcccct tacggcacc aagtggttc caactctac 
      atctacaac gaccgaaac gtaacccgg cggctggtc 
      gaacgtgcc gaaaaggcc ggtttcaaa gccttagtc 
      ctcaccgtg gacaccccc atgttcggg ctgcgcctt 
      gccgacatc cgcaacaag ttcgtcttg cccccacac 
      ctaaaattc gccaatttc gcgggcgac aaagccacc 
      ggtattaac caaaccgag tccggttcg ggtctcaac 
      aactacgtg aaccggttg tttgaccaa agcttggag 
      tggaaagac atcaagtgg ctccaaagc ttcaccaaa 
      ctccccatt gttgttaaa ggggtccta acggccgag 
      gacgccctc attgccgct gatttgggg gtgcagggg 
      atcctggtc tcgaaccat ggggcgaga caggtcgat 
      gggactcca gcctccatt gaggctctc cccgaaatt 
      gtgcgagcg gttggggac agagtcgag gtttacatg 
      gatgggggc attactgat gggactgat attttcaag 
      gctttagca ttgggtgcc cggatggtg tttttcgga 
      aggccggcc ctgtggggc ctggcgcac agtggggaa 
      gaaggggtc aagaaaatc ctgaatata ctcaaaact 
      gagctggat tacaccatg gccattact gggtgtgcc 
      actgtgagg gatattgac cacaggatg gtcgtacat 
      gagagttat tacagcaaa ttgtaa
    mRNA sequence
    • >TC030629 RA
      cgaacattc agtcgccac aaaaccacc aataagaaa 
      aaatgagcg aagcaatag tttgcgtca aagactttg 
      aaaaacatg cttacaacg tcctgcccc gaaatgcgc 
      tcgattact atcgaagtg gggccggag ctgaagaga 
      ctctggctc ataacagga aagctttct ccaagtaca 
      aaattcggc cgcgatgtt taagaaacg tggccaaaa 
      gagacctct cgaccacag ttttgggcg agaaagtgc 
      aaatcccgg tcgggattt cccccacag ccatgcaga 
      ggatggcgc acccagagg gcgagtgtg ccaatgcac 
      gagctgctc aggcaatgg gcaccatct tcactctaa 
      gcaccatcg caactagca gcatcgaag aagtggccc 
      aagcggccc cttacggca ccaagtggt tccaactct 
      acatctaca acgaccgaa acgtaaccc ggcggctgg 
      tcgaacgtg ccgaaaagg ccggtttca aagccttag 
      tcctcaccg tggacaccc ccatgttcg ggctgcgcc 
      ttgccgaca tccgcaaca agttcgtct tgcccccac 
      acctaaaat tcgccaatt tcgcgggcg acaaagcca 
      ccggtatta accaaaccg agtccggtt cgggtctca 
      acaactacg tgaaccggt tgtttgacc aaagcttgg 
      agtggaaag acatcaagt ggctccaaa gcttcacca 
      aactcccca ttgttgtta aaggggtcc taacggccg 
      aggacgccc tcattgccg ctgatttgg gggtgcagg 
      ggatcctgg tctcgaacc atggggcga gacaggtcg 
      atgggactc cagcctcca ttgaggctc tccccgaaa 
      ttgtgcgag cggttgggg acagagtcg aggtttaca 
      tggatgggg gcattactg atgggactg atattttca 
      aggctttag cattgggtg cccggatgg tgtttttcg 
      gaaggccgg ccctgtggg gcctggcgc acagtgggg 
      aagaagggg tcaagaaaa tcctgaata tactcaaaa 
      ctgagctgg attacacca tggccatta ctgggtgtg 
      ccactgtga gggatattg accacagga tggtcgtac 
      atgagagtt attacagca aattgtaaa aaatgtgat 
      tatgtaata ggataggca accaaatat acgttcggt 
      cccaag