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Gene information
General information
TC030625 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 1971641..1972675
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030625
    iBeetle screen iB_02601

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 9 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030625 RA
      MALAEICGM SDFDTFRRE APFDNEIAK MSNNFTHNT 
      LLSVPPYSN PAENLAYFS KDESGRDIK IKFDHGTTT 
      LGFMYKGGV VLAVDSRAT GGQFIGSQT MKKIVEIND 
      FLLGTLAGG AADCVYWDR VLAKQCRMY ELRNRERIS 
      VAAASKLMA NMVYNYKGM GLSMGMMLA GWDKRGPQL 
      YYVDSEGTR TPGKVFSVG SGSIYAFGV LDSGYKWDL 
      TDEEAYDLG RRSIYHATH RDAYSGGIV RVYHMKPTG 
      WVHIDNNDC MDLHYKYQD EKLQEINKV
    CDS sequence
    • >TC030625 RA
      atggcttta gccgagatt tgcggaatg tcggacttc 
      gacaccttc agacgcgaa gcccccttc gacaacgaa 
      atcgccaaa atgagcaac aacttcacc cacaacact 
      ttgctcagc gtacccccc tattctaac ccggccgag 
      aacctggcc tacttctcg aaggacgag tcaggtcgg 
      gacatcaaa atcaagttt gaccacggc accaccacg 
      ctggggttc atgtacaag gggggcgta gtgctcgcc 
      gtggactcc cgagccacg gggggccaa ttcatcggc 
      tcccaaacc atgaagaaa atcgtcgaa atcaatgat 
      tttctcctg gggactttg gccgggggc gccgctgac 
      tgtgtgtat tgggaccgc gtcctggct aagcagtgc 
      cgcatgtac gaattacgc aaccgggag cggatttcg 
      gtcgcagcc gcctcgaaa ctgatggcc aacatggtg 
      tataattac aaggggatg gggctgtca atgggcatg 
      atgctggca ggatgggac aaaaggggg ccccagctg 
      tattacgtg gattcggag gggaccaga acccctggg 
      aaagtcttc agtgttggc tcgggctcc atttatgcg 
      tttggcgtc ctggattcg gggtacaag tgggatttg 
      acggatgag gaggcgtat gatttgggg cgccggtcg 
      atttaccat gccacccat cgggacgcc tattctggt 
      ggtatcgtc agggtgtac catatgaag ccgaccggc 
      tgggtgcat attgataac aacgactgt atggacttg 
      cactacaaa taccaggac gagaagttg caggaaata 
      aataaggtt tag
    mRNA sequence
    • >TC030625 RA
      tagaggtta ggtgctagt tgtgttttc gcaccgtca 
      tttgaacca aacaaaact gccccaaaa tggctttag 
      ccgagattt gcggaatgt cggacttcg acaccttca 
      gacgcgaag cccccttcg acaacgaaa tcgccaaaa 
      tgagcaaca acttcaccc acaacactt tgctcagcg 
      tacccccct attctaacc cggccgaga acctggcct 
      acttctcga aggacgagt caggtcggg acatcaaaa 
      tcaagtttg accacggca ccaccacgc tggggttca 
      tgtacaagg ggggcgtag tgctcgccg tggactccc 
      gagccacgg ggggccaat tcatcggct cccaaacca 
      tgaagaaaa tcgtcgaaa tcaatgatt ttctcctgg 
      ggactttgg ccgggggcg ccgctgact gtgtgtatt 
      gggaccgcg tcctggcta agcagtgcc gcatgtacg 
      aattacgca accgggagc ggatttcgg tcgcagccg 
      cctcgaaac tgatggcca acatggtgt ataattaca 
      aggggatgg ggctgtcaa tgggcatga tgctggcag 
      gatgggaca aaagggggc cccagctgt attacgtgg 
      attcggagg ggaccagaa cccctggga aagtcttca 
      gtgttggct cgggctcca tttatgcgt ttggcgtcc 
      tggattcgg ggtacaagt gggatttga cggatgagg 
      aggcgtatg atttggggc gccggtcga tttaccatg 
      ccacccatc gggacgcct attctggtg gtatcgtca 
      gggtgtacc atatgaagc cgaccggct gggtgcata 
      ttgataaca acgactgta tggacttgc actacaaat 
      accaggacg agaagttgc aggaaataa ataaggttt 
      agctttgta atgaattaa attgaataa attttttta 
      acatag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY