| General information | |||
| TC030607 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 12159968..12163302 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC030607 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02595 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
- >TC030607 RA
MLGPGLGKK RTKGSTGIT GGRMKVDAA SQRERLEQM LSRRTLECL VCCEKIKHT DKIWTCQQC YHIFHLNCT IAWANSSKL DSGWRCPAC QNVCSDIPK QYTCYCGKT LEPRPLPGV VPHACGELC EKTRRCDHK CTILCHPGP CPDCNVMVA KPCGCGATT QTVKCSADT EIVCGAICG KKLSCGVHE CGAKCHVGP CMECDRELR QICYCEKVG RKVKCREEF KGAVNYSCG EVCGRGLSC GNHTCTKVC HEGDCEACP RDVGVVKTC PCGRSELKK RRESCLDPI PCCDKICGK ILNCGQPAA PHSCKLPCH EGPCPPCPL TTLIRCRCG HMDREIPCG QVTTKADDA RCEKKCTKK RLCGKHRCN QRCCIEIEH ICPLPCNHM LACGQHRCE RTCHSGRCP PCVETSFDE LYCECGANV LYPPIPCGT KPPPCNNPC SRPRPCGHE ANHACHTGA CPPCTVLCK RWCYGKHEQ RSAIPCYQE DFSCGLPCG KAMPCGAHK CDKACHLGA CTLPCKQKC NVKRVQCGH PCGVTCHEG PCPDTPCRQ MVPVTCPCG LQKATRPCI ELAEAYKEI EMAHLKDKM ADLLKDQAV DISDINKAK RPSVLKILE CTEECRVLE RNRRLAIGL QIRNPDLSQ KLTPRYSDF MRNWAKKDP QFCQKVHEK LTELVQLAK QSKQKSRSY SFESMNRDK RHFVHEYCE HFGCESAAY DAEPNRNIV ATAFKDKSW LPSMSLLEF IQRENGQRK VPGPVLSKG TLGKSETVA LKLPGRGQR GTPSGETVD YFDNPPV
- >TC030607 RB
MSYWGPYED QLNGGFPGC NPEYVQYFN NMSYYNDPN SANYSAVNY YDGQTTYQN NYLEQPGTS QVTNNWAPN HSNEDVGAG TRKKTNERK YWNNGRKNE RYGSGRTFT RSKPEQRKY PENDEKVDK FNQKQDGRS QNSNTRFRN NYRSRDKYD FYDGRGYGK YGKSSRSYE DNGASGRDW RSSNANRKC ASNVQKKFD AASQRERLE QMLSRRTLE CLVCCEKIK HTDKIWTCQ QCYHIFHLN CTIAWANSS KLDSGWRCP ACQNVCSDI PKQYTCYCG KTLEPRPLP GVVPHACGE LCEKTRRCD HKCTILCHP GPCPDCNVM VAKPCGCGA TTQTVKCSA DTEIVCGAI CGKKLSCGV HECGAKCHV GPCMECDRE LRQICYCEK VGRKVKCRE EFKGAVNYS CGEVCGRGL SCGNHTCTK VCHEGDCEA CPRDVGVVK TCPCGRSEL KKRRESCLD PIPCCDKIC GKILNCGQP AAPHSCKLP CHEGPCPPC PLTTLIRCR CGHMDREIP CGQVTTKAD DARCEKKCT KKRLCGKHR CNQRCCIEI EHICPLPCN HMLACGQHR CERTCHSGR CPPCVETSF DELYCECGA NVLYPPIPC GTKPPPCNN PCSRPRPCG HEANHACHT GACPPCTVL CKRWCYGKH EQRSAIPCY QEDFSCGLP CGKAMPCGA HKCDKACHL GACTLPCKQ KCNVKRVQC GHPCGVTCH EGPCPDTPC RQMVPVTCP CGLQKATRP CIELAEAYK EIEMAHLKD KMADLLKDQ AVDISDINK AKRPSVLKI LECTEECRV LERNRRLAI GLQIRNPDL SQKLTPRYS DFMRNWAKK DPQFCQKVH EKLTELVQL AKQSKQKSR SYSFESMNR DKRHFVHEY CEHFGCESA AYDAEPNRN IVATAFKDK SWLPSMSLL EFIQRENGQ RKVPGPVLS KGTLGKSET VALKLPGRG QRGTPSGET VDYFDNPPV
- >TC030607 RA
atgttgggg ccgggacta ggaaaaaaa cgaacgaaa ggaagtact ggaataacg ggaggaaga atgaaagtt gacgccgcc tctcagcgc gagcgcctc gagcagatg ctctcgcgt cgcacgctg gagtgcctc gtctgttgc gaaaaaatc aaacacact gacaaaatc tggacttgt cagcagtgt taccacatc ttccatctc aactgcacc atcgcctgg gccaattcg tcaaaacta gattcaggc tggcgttgt ccagcttgt caaaacgtt tgcagcgac attcccaaa cagtacact tgctactgc ggcaaaacg ctcgaaccg cgccccctt ccaggcgta gtcccgcac gcttgtggc gaattatgc gaaaaaacg cgccgttgt gaccacaaa tgcaccatt ctgtgccac ccggggccc tgtcccgac tgcaacgtc atggtggca aagccctgc ggttgtggg gccaccaca caaacagtc aaatgtagc gctgatacc gaaattgtt tgtggcgct atctgtgga aaaaaactc tcatgtggg gtccacgaa tgcggggcg aagtgccac gtggggcca tgtatggaa tgcgataga gagttgaga cagatttgt tattgtgag aaggtgggg cgtaaagtg aaatgtagg gaggagttt aaaggagcg gtgaattat tcgtgtggg gaggtttgt gggcggggc ttgagttgt gggaatcac acgtgcact aaagtgtgt cacgagggg gattgtgag gcgtgtccg agagatgtg ggggtggtt aagacgtgt ccttgtggg aggagcgaa ttgaagaag agaagagaa tcatgtctg gatcctata ccgtgctgt gataaaatt tgtgggaaa atcctaaat tgtgggcaa ccggcagcc ccccactcc tgcaaactt ccatgtcac gagggtccg tgcccccca tgccccctt acgaccttg attcgttgt cgttgcggt cacatggac cgggaaatc ccctgcgga caagtaacg acgaaagcc gacgatgcc cgatgtgag aaaaaatgt accaagaaa cgcctctgt ggcaaacac cgatgcaat caacgttgt tgcatcgaa atcgaacac atttgtccc ctcccatgc aatcacatg ctcgcttgt ggccaacac cgttgcgag aggacgtgt cacagtggg cggtgccca ccttgcgtg gaaacaagt ttcgacgaa ttgtattgc gaatgcgga gctaacgtc ttatacccg cccattcca tgcggcaca aaaccgccc ccttgcaat aatccatgt tccaggcca cgcccatgt ggccacgaa gccaatcat gcgtgccac acaggggcg tgtccaccg tgtaccgtt ttgtgcaag aggtggtgt tacggcaaa cacgagcag agatcggct attccctgt tatcaggag gatttcagt tgtggatta ccgtgtggg aaggccatg ccgtgtggg gcacataag tgtgacaag gcgtgtcat ttgggggcg tgtacgttg ccatgcaaa cagaaatgt aacgtgaag agagtccaa tgcgggcat ccgtgcggg gtcacgtgt cacgaaggg ccgtgtcct gacacgccc tgtaggcaa atggtgccg gtcacgtgc ccctgtggc ttgcagaaa gcaacaagg ccttgcatc gagttggct gaggcgtat aaggagatc gaaatggcg catttgaag gacaaaatg gccgatttg ttgaaggat caagctgtg gacatcagc gatatcaac aaagccaaa aggccgtcg gttttgaaa atattggag tgcaccgaa gagtgtcgg gttctggag cgcaaccgg cggctggct atcggtctg cagattcgc aacccggat ttgagtcaa aaactgact cctcgttac tccgatttt atgcgaaat tgggcgaaa aaagacccg cagttttgc caaaaagtg cacgaaaag ctcaccgag ttggtccaa ttggctaag cagagtaaa caaaagagt cgttcgtat tcttttgag tcaatgaac agagataag agacatttc gtgcacgag tattgtgag cattttggg tgtgaaagc gccgcctac gatgcggaa cccaaccga aatatcgtt gcaactgcc tttaaggac aagtcgtgg ttgccgagt atgagtcta ttggagttt attcagagg gaaaatggg cagaggaag gttccaggg ccggttctg agcaaaggg acgttgggg aagtcggag accgttgcc ttgaaattg cctggaagg gggcagagg ggaacgcca tcaggagag accgtggat tatttcgat aacccccca gtgtaa
- >TC030607 RB
atgtcgtat tggggcccc tacgaagat cagttgaat ggcggattc ccaggatgt aatccagaa tatgtccaa tatttcaac aacatgtct tattacaat gatcccaac agtgccaat tacagtgcc gtaaattac tatgacggt caaacaacg taccagaat aactatttg gagcagcct ggaaccagt caggtgact aataactgg gccccaaat cactctaat gaggatgtt ggggccggg actaggaaa aaaacgaac gaaaggaag tactggaat aacgggagg aagaatgaa aggtacggg agtggtcgc acgtttact agaagtaag cctgagcaa aggaagtat ccagaaaat gatgaaaaa gtagataag tttaatcag aagcaagac gggaggagt caaaatagt aacactagg tttaggaac aattataga agtagggat aagtatgat ttttacgac gggagaggg tatgggaaa tacgggaag tctagtagg agttacgaa gataacggg gccagtgga cgggactgg cgctccagt aacgcaaat cgcaagtgc gccagcaac gtgcaaaag aaatttgac gccgcctct cagcgcgag cgcctcgag cagatgctc tcgcgtcgc acgctggag tgcctcgtc tgttgcgaa aaaatcaaa cacactgac aaaatctgg acttgtcag cagtgttac cacatcttc catctcaac tgcaccatc gcctgggcc aattcgtca aaactagat tcaggctgg cgttgtcca gcttgtcaa aacgtttgc agcgacatt cccaaacag tacacttgc tactgcggc aaaacgctc gaaccgcgc ccccttcca ggcgtagtc ccgcacgct tgtggcgaa ttatgcgaa aaaacgcgc cgttgtgac cacaaatgc accattctg tgccacccg gggccctgt cccgactgc aacgtcatg gtggcaaag ccctgcggt tgtggggcc accacacaa acagtcaaa tgtagcgct gataccgaa attgtttgt ggcgctatc tgtggaaaa aaactctca tgtggggtc cacgaatgc ggggcgaag tgccacgtg gggccatgt atggaatgc gatagagag ttgagacag atttgttat tgtgagaag gtggggcgt aaagtgaaa tgtagggag gagtttaaa ggagcggtg aattattcg tgtggggag gtttgtggg cggggcttg agttgtggg aatcacacg tgcactaaa gtgtgtcac gagggggat tgtgaggcg tgtccgaga gatgtgggg gtggttaag acgtgtcct tgtgggagg agcgaattg aagaagaga agagaatca tgtctggat cctataccg tgctgtgat aaaatttgt gggaaaatc ctaaattgt gggcaaccg gcagccccc cactcctgc aaacttcca tgtcacgag ggtccgtgc cccccatgc ccccttacg accttgatt cgttgtcgt tgcggtcac atggaccgg gaaatcccc tgcggacaa gtaacgacg aaagccgac gatgcccga tgtgagaaa aaatgtacc aagaaacgc ctctgtggc aaacaccga tgcaatcaa cgttgttgc atcgaaatc gaacacatt tgtcccctc ccatgcaat cacatgctc gcttgtggc caacaccgt tgcgagagg acgtgtcac agtgggcgg tgcccacct tgcgtggaa acaagtttc gacgaattg tattgcgaa tgcggagct aacgtctta tacccgccc attccatgc ggcacaaaa ccgccccct tgcaataat ccatgttcc aggccacgc ccatgtggc cacgaagcc aatcatgcg tgccacaca ggggcgtgt ccaccgtgt accgttttg tgcaagagg tggtgttac ggcaaacac gagcagaga tcggctatt ccctgttat caggaggat ttcagttgt ggattaccg tgtgggaag gccatgccg tgtggggca cataagtgt gacaaggcg tgtcatttg ggggcgtgt acgttgcca tgcaaacag aaatgtaac gtgaagaga gtccaatgc gggcatccg tgcggggtc acgtgtcac gaagggccg tgtcctgac acgccctgt aggcaaatg gtgccggtc acgtgcccc tgtggcttg cagaaagca acaaggcct tgcatcgag ttggctgag gcgtataag gagatcgaa atggcgcat ttgaaggac aaaatggcc gatttgttg aaggatcaa gctgtggac atcagcgat atcaacaaa gccaaaagg ccgtcggtt ttgaaaata ttggagtgc accgaagag tgtcgggtt ctggagcgc aaccggcgg ctggctatc ggtctgcag attcgcaac ccggatttg agtcaaaaa ctgactcct cgttactcc gattttatg cgaaattgg gcgaaaaaa gacccgcag ttttgccaa aaagtgcac gaaaagctc accgagttg gtccaattg gctaagcag agtaaacaa aagagtcgt tcgtattct tttgagtca atgaacaga gataagaga catttcgtg cacgagtat tgtgagcat tttgggtgt gaaagcgcc gcctacgat gcggaaccc aaccgaaat atcgttgca actgccttt aaggacaag tcgtggttg ccgagtatg agtctattg gagtttatt cagagggaa aatgggcag aggaaggtt ccagggccg gttctgagc aaagggacg ttggggaag tcggagacc gttgccttg aaattgcct ggaaggggg cagagggga acgccatca ggagagacc gtggattat ttcgataac cccccagtg taa
- >TC030607 RA
ctctgtcat ttttccaac tgtcaaaat ttaagctca accatcaat ctcccgcac gtaatttgt tgcaacaaa ttacacaac tgagcgaaa ttgttgcac taaacgtat gtcgtattg gggccccta cgaagatca gttgaatgg cggattccc aggatgtaa tccagaata tgtccaata tttcaacaa catgtctta ttacaatga tcccaacag tgccaatta cagtgccgt aaattacta tgacggtca aacaacgta ccagaataa ctatttgga gcagcctgg aaccagtca ggtgactaa taactgggc cccaaatca ctctaatga ggatgttgg ggccgggac taggaaaaa aacgaacga aaggaagta ctggaataa cgggaggaa gaatgaaag ttgacgccg cctctcagc gcgagcgcc tcgagcaga tgctctcgc gtcgcacgc tggagtgcc tcgtctgtt gcgaaaaaa tcaaacaca ctgacaaaa tctggactt gtcagcagt gttaccaca tcttccatc tcaactgca ccatcgcct gggccaatt cgtcaaaac tagattcag gctggcgtt gtccagctt gtcaaaacg tttgcagcg acattccca aacagtaca cttgctact gcggcaaaa cgctcgaac cgcgccccc ttccaggcg tagtcccgc acgcttgtg gcgaattat gcgaaaaaa cgcgccgtt gtgaccaca aatgcacca ttctgtgcc acccggggc cctgtcccg actgcaacg tcatggtgg caaagccct gcggttgtg gggccacca cacaaacag tcaaatgta gcgctgata ccgaaattg tttgtggcg ctatctgtg gaaaaaaac tctcatgtg gggtccacg aatgcgggg cgaagtgcc acgtggggc catgtatgg aatgcgata gagagttga gacagattt gttattgtg agaaggtgg ggcgtaaag tgaaatgta gggaggagt ttaaaggag cggtgaatt attcgtgtg gggaggttt gtgggcggg gcttgagtt gtgggaatc acacgtgca ctaaagtgt gtcacgagg gggattgtg aggcgtgtc cgagagatg tgggggtgg ttaagacgt gtccttgtg ggaggagcg aattgaaga agagaagag aatcatgtc tggatccta taccgtgct gtgataaaa tttgtggga aaatcctaa attgtgggc aaccggcag ccccccact cctgcaaac ttccatgtc acgagggtc cgtgccccc catgccccc ttacgacct tgattcgtt gtcgttgcg gtcacatgg accgggaaa tcccctgcg gacaagtaa cgacgaaag ccgacgatg cccgatgtg agaaaaaat gtaccaaga aacgcctct gtggcaaac accgatgca atcaacgtt gttgcatcg aaatcgaac acatttgtc ccctcccat gcaatcaca tgctcgctt gtggccaac accgttgcg agaggacgt gtcacagtg ggcggtgcc caccttgcg tggaaacaa gtttcgacg aattgtatt gcgaatgcg gagctaacg tcttatacc cgcccattc catgcggca caaaaccgc ccccttgca ataatccat gttccaggc cacgcccat gtggccacg aagccaatc atgcgtgcc acacagggg cgtgtccac cgtgtaccg ttttgtgca agaggtggt gttacggca aacacgagc agagatcgg ctattccct gttatcagg aggatttca gttgtggat taccgtgtg ggaaggcca tgccgtgtg gggcacata agtgtgaca aggcgtgtc atttggggg cgtgtacgt tgccatgca aacagaaat gtaacgtga agagagtcc aatgcgggc atccgtgcg gggtcacgt gtcacgaag ggccgtgtc ctgacacgc cctgtaggc aaatggtgc cggtcacgt gcccctgtg gcttgcaga aagcaacaa ggccttgca tcgagttgg ctgaggcgt ataaggaga tcgaaatgg cgcatttga aggacaaaa tggccgatt tgttgaagg atcaagctg tggacatca gcgatatca acaaagcca aaaggccgt cggttttga aaatattgg agtgcaccg aagagtgtc gggttctgg agcgcaacc ggcggctgg ctatcggtc tgcagattc gcaacccgg atttgagtc aaaaactga ctcctcgtt actccgatt ttatgcgaa attgggcga aaaaagacc cgcagtttt gccaaaaag tgcacgaaa agctcaccg agttggtcc aattggcta agcagagta aacaaaaga gtcgttcgt attcttttg agtcaatga acagagata agagacatt tcgtgcacg agtattgtg agcattttg ggtgtgaaa gcgccgcct acgatgcgg aacccaacc gaaatatcg ttgcaactg cctttaagg acaagtcgt ggttgccga gtatgagtc tattggagt ttattcaga gggaaaatg ggcagagga aggttccag ggccggttc tgagcaaag ggacgttgg ggaagtcgg agaccgttg ccttgaaat tgcctggaa gggggcaga ggggaacgc catcaggag agaccgtgg attatttcg ataaccccc cagtgtaat gttttataa cgtattatg tttagtatt tttgaaggt taataaatt ggtt
- >TC030607 RB
ctctgtcat ttttccaac tgtcaaaat ttaagctca accatcaat ctcccgcac gtaatttgt tgcaacaaa ttacacaac tgagcgaaa ttgttgcac taaacgtat gtcgtattg gggccccta cgaagatca gttgaatgg cggattccc aggatgtaa tccagaata tgtccaata tttcaacaa catgtctta ttacaatga tcccaacag tgccaatta cagtgccgt aaattacta tgacggtca aacaacgta ccagaataa ctatttgga gcagcctgg aaccagtca ggtgactaa taactgggc cccaaatca ctctaatga ggatgttgg ggccgggac taggaaaaa aacgaacga aaggaagta ctggaataa cgggaggaa gaatgaaag gtacgggag tggtcgcac gtttactag aagtaagcc tgagcaaag gaagtatcc agaaaatga tgaaaaagt agataagtt taatcagaa gcaagacgg gaggagtca aaatagtaa cactaggtt taggaacaa ttatagaag tagggataa gtatgattt ttacgacgg gagagggta tgggaaata cgggaagtc tagtaggag ttacgaaga taacggggc cagtggacg ggactggcg ctccagtaa cgcaaatcg caagtgcgc cagcaacgt gcaaaagaa atttgacgc cgcctctca gcgcgagcg cctcgagca gatgctctc gcgtcgcac gctggagtg cctcgtctg ttgcgaaaa aatcaaaca cactgacaa aatctggac ttgtcagca gtgttacca catcttcca tctcaactg caccatcgc ctgggccaa ttcgtcaaa actagattc aggctggcg ttgtccagc ttgtcaaaa cgtttgcag cgacattcc caaacagta cacttgcta ctgcggcaa aacgctcga accgcgccc ccttccagg cgtagtccc gcacgcttg tggcgaatt atgcgaaaa aacgcgccg ttgtgacca caaatgcac cattctgtg ccacccggg gccctgtcc cgactgcaa cgtcatggt ggcaaagcc ctgcggttg tggggccac cacacaaac agtcaaatg tagcgctga taccgaaat tgtttgtgg cgctatctg tggaaaaaa actctcatg tggggtcca cgaatgcgg ggcgaagtg ccacgtggg gccatgtat ggaatgcga tagagagtt gagacagat ttgttattg tgagaaggt ggggcgtaa agtgaaatg tagggagga gtttaaagg agcggtgaa ttattcgtg tggggaggt ttgtgggcg gggcttgag ttgtgggaa tcacacgtg cactaaagt gtgtcacga gggggattg tgaggcgtg tccgagaga tgtgggggt ggttaagac gtgtccttg tgggaggag cgaattgaa gaagagaag agaatcatg tctggatcc tataccgtg ctgtgataa aatttgtgg gaaaatcct aaattgtgg gcaaccggc agcccccca ctcctgcaa acttccatg tcacgaggg tccgtgccc cccatgccc ccttacgac cttgattcg ttgtcgttg cggtcacat ggaccggga aatcccctg cggacaagt aacgacgaa agccgacga tgcccgatg tgagaaaaa atgtaccaa gaaacgcct ctgtggcaa acaccgatg caatcaacg ttgttgcat cgaaatcga acacatttg tcccctccc atgcaatca catgctcgc ttgtggcca acaccgttg cgagaggac gtgtcacag tgggcggtg cccaccttg cgtggaaac aagtttcga cgaattgta ttgcgaatg cggagctaa cgtcttata cccgcccat tccatgcgg cacaaaacc gcccccttg caataatcc atgttccag gccacgccc atgtggcca cgaagccaa tcatgcgtg ccacacagg ggcgtgtcc accgtgtac cgttttgtg caagaggtg gtgttacgg caaacacga gcagagatc ggctattcc ctgttatca ggaggattt cagttgtgg attaccgtg tgggaaggc catgccgtg tggggcaca taagtgtga caaggcgtg tcatttggg ggcgtgtac gttgccatg caaacagaa atgtaacgt gaagagagt ccaatgcgg gcatccgtg cggggtcac gtgtcacga agggccgtg tcctgacac gccctgtag gcaaatggt gccggtcac gtgcccctg tggcttgca gaaagcaac aaggccttg catcgagtt ggctgaggc gtataagga gatcgaaat ggcgcattt gaaggacaa aatggccga tttgttgaa ggatcaagc tgtggacat cagcgatat caacaaagc caaaaggcc gtcggtttt gaaaatatt ggagtgcac cgaagagtg tcgggttct ggagcgcaa ccggcggct ggctatcgg tctgcagat tcgcaaccc ggatttgag tcaaaaact gactcctcg ttactccga ttttatgcg aaattgggc gaaaaaaga cccgcagtt ttgccaaaa agtgcacga aaagctcac cgagttggt ccaattggc taagcagag taaacaaaa gagtcgttc gtattcttt tgagtcaat gaacagaga taagagaca tttcgtgca cgagtattg tgagcattt tgggtgtga aagcgccgc ctacgatgc ggaacccaa ccgaaatat cgttgcaac tgcctttaa ggacaagtc gtggttgcc gagtatgag tctattgga gtttattca gagggaaaa tgggcagag gaaggttcc agggccggt tctgagcaa agggacgtt ggggaagtc ggagaccgt tgccttgaa attgcctgg aagggggca gaggggaac gccatcagg agagaccgt ggattattt cgataaccc cccagtgta atgttttat aacgtatta tgtttagta tttttgaag gttaataaa ttggtt
