General information | |||
TC030578 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 6645026..6669700 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC030578 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02592 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC030578 RA
MLCFVQGFE DRDDGKERK LQVVKNRFL KKSRSSGNF GSAGARSAP GTPQGTLGT SPEPVSQPC SPGCTSPSI QRSPSHSLF TKCDKASLK HLSTSTPAM TAQAQLTEP PSPKTAKEN KVPTTSKAR QKKFNRHFP AVDDDEKVL NHYSCALIG DILLQGHLY ITKNYFAFY SNVFGYVTK LLIPMLSVE KITKEKTAR IIPNAVGIA TSEDKHVFG SLMSRDSTY RYMVKVWEV AQNASLLVV EPEIVEPVV SDSDSTETR EGESGRESP LPTPRFAQM VLKEHKDTA ATGPKVENQ QRSMELLYA LKQAISKFA SLPRQSLIL FATTLLLIL LFLSAAVLL YRISKIQSK YSFNLHETM LAAGSEDVY SNLLQYQTH LHSKSAGAV HNFLDTNLD QIAKVRQSL EALSTLLIP NNRQKEASS RDSSS
- >TC030578 RA
atgttgtgt ttcgtgcaa ggattcgag gatagagac gatggcaaa gagcgcaag ctgcaagtg gtcaagaat cgcttcttg aaaaaatca cggtcgtct ggcaatttt gggagcgcg ggggcccgg tcggctccc ggaacccca caagggaca ctcggtact tccccggaa cctgtctcg cagccatgc agtccgggc tgcacatcg ccctcgatc cagcgaagt ccaagtcac tcgctcttc actaaatgc gataaagcg tctttgaaa cacttatcg acgtcgacg ccggccatg acggcccaa gcgcagctc accgaaccc cccagcccc aaaacggca aaggagaac aaagtgcct acgacgtcg aaagcgcgc cagaaaaaa ttcaacaga cactttccg gctgttgac gatgacgag aaagtccta aatcattat tcctgtgcc cttatcggt gatattctg ctacaaggc cacttgtac atcaccaaa aactacttt gcgttctat tccaacgtc ttcggatac gtgaccaag cttctcatt ccaatgctg tccgtggag aaaataacg aaagaaaaa accgcaagg ataatcccc aacgctgtt ggaatcgcc acaagcgag gacaagcac gttttcggc agcctaatg tcacgtgac agcacctac cgttacatg gtcaaagtc tgggaggtg gcccagaac gcctctctc ctcgtcgtc gagccggaa attgtggaa cctgtagtg tcggacagc gacagcacg gagacgcgc gaaggcgaa agcggccga gagtcgcct cttcccact ccccgattt gctcagatg gtcctcaag gagcacaag gacaccgct gccacaggg cctaaggtc gaaaaccaa caacgctca atggaactg ctctacgct ctcaagcag gccatctcg aaatttgcc agcctaccg cgacagagc ctaatactg ttcgccacc acgttgctc ctcattctc ctcttctta tcggctgct gtgctactt tatcgaatt agtaaaata caaagcaaa tattcgttc aatttgcat gagacgatg ctcgctgcg ggcagcgaa gacgtctac agcaatctg ctgcaatat cagacgcac ttgcattcg aagtcggcg ggggctgtg cacaacttc ttagacacg aatttagac caaattgcg aaggtgaga cagtccctg gaagcgcta tcgaccctg ttgatcccc aacaacagg cagaaagaa gcgagttcc agagacagc agcagttag
- >TC030578 RA
ctgccctcc acaacaacc gcttgacga cgtgtataa taccgttcg ttcgactcg caacctcac caagcgtga acacattca gtcaatgaa gagaccgcg aaaggggca acgttttcg cctgccatg tgcccaaaa agtcgctaa agtcgcctc tgaatcact taaaaaaaa tcgccacca tgtcgccaa atcctgagt gatgttgtg tttcgtgca aggattcga ggatagaga cgatggcaa agagcgcaa gctgcaagt ggtcaagaa tcgcttctt gaaaaaatc acggtcgtc tggcaattt tgggagcgc gggggcccg gtcggctcc cggaacccc acaagggac actcggtac ttccccgga acctgtctc gcagccatg cagtccggg ctgcacatc gccctcgat ccagcgaag tccaagtca ctcgctctt cactaaatg cgataaagc gtctttgaa acacttatc gacgtcgac gccggccat gacggccca agcgcagct caccgaacc ccccagccc caaaacggc aaaggagaa caaagtgcc tacgacgtc gaaagcgcg ccagaaaaa attcaacag acactttcc ggctgttga cgatgacga gaaagtcct aaatcatta ttcctgtgc ccttatcgg tgatattct gctacaagg ccacttgta catcaccaa aaactactt tgcgttcta ttccaacgt cttcggata cgtgaccaa gcttctcat tccaatgct gtccgtgga gaaaataac gaaagaaaa aaccgcaag gataatccc caacgctgt tggaatcgc cacaagcga ggacaagca cgttttcgg cagcctaat gtcacgtga cagcaccta ccgttacat ggtcaaagt ctgggaggt ggcccagaa cgcctctct cctcgtcgt cgagccgga aattgtgga acctgtagt gtcggacag cgacagcac ggagacgcg cgaaggcga aagcggccg agagtcgcc tcttcccac tccccgatt tgctcagat ggtcctcaa ggagcacaa ggacaccgc tgccacagg gcctaaggt cgaaaacca acaacgctc aatggaact gctctacgc tctcaagca ggccatctc gaaatttgc cagcctacc gcgacagag cctaatact gttcgccac cacgttgct cctcattct cctcttctt atcggctgc tgtgctact ttatcgaat tagtaaaat acaaagcaa atattcgtt caatttgca tgagacgat gctcgctgc gggcagcga agacgtcta cagcaatct gctgcaata tcagacgca cttgcattc gaagtcggc gggggctgt gcacaactt cttagacac gaatttaga ccaaattgc gaaggtgag acagtccct ggaagcgct atcgaccct gttgatccc caacaacag gcagaaaga agcgagttc cagagacag cagcagtta gccacgtta ataacagtg ctaggaatg tgatcagta ttcaatctt ttaaattta gatttagca atatttcac ttaaatatt gttgccacc atcccagta tcgacacta ggccgcgct gtttcatca tatttgcca aatctatta ttgccataa gggtaatgg aactactcc tatgtatag tccgcacgt ttatactat aagctcaat ttgtcacga ggggttttt ggtgtaatg gaccgatga ttgtaaact gggacttgt ttttaatag ttaattaat ttatcgcca tctagtaca aacttattt tataattcc actttagtt caacgcgcc caacactgt ctagcaata atatcaaag gatttttta aaaaaaaag attgggaac taatgctgt gaattggta ctgagcgat tttcgtaat tttagaaac ttcgtaatc ctgattagg cagaaactt gttgaaacg ttcatcctt tatttcaat gtacagttt aataggaac gatttacac tgtttttaa ttttacatg tacctacga tgtttatat gttaggtgt taatttatt gcggttaga gttgtgttt tgtcaattg ttttgtaac atgttaaca tagaagaaa cctgtgata tcttatttg ttttgcttt tatcaataa atagttaca atttaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
