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Gene information
General information
TC030578 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6645026..6669700
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030578
    iBeetle screen iB_02592

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030578 RA
      MLCFVQGFE DRDDGKERK LQVVKNRFL KKSRSSGNF 
      GSAGARSAP GTPQGTLGT SPEPVSQPC SPGCTSPSI 
      QRSPSHSLF TKCDKASLK HLSTSTPAM TAQAQLTEP 
      PSPKTAKEN KVPTTSKAR QKKFNRHFP AVDDDEKVL 
      NHYSCALIG DILLQGHLY ITKNYFAFY SNVFGYVTK 
      LLIPMLSVE KITKEKTAR IIPNAVGIA TSEDKHVFG 
      SLMSRDSTY RYMVKVWEV AQNASLLVV EPEIVEPVV 
      SDSDSTETR EGESGRESP LPTPRFAQM VLKEHKDTA 
      ATGPKVENQ QRSMELLYA LKQAISKFA SLPRQSLIL 
      FATTLLLIL LFLSAAVLL YRISKIQSK YSFNLHETM 
      LAAGSEDVY SNLLQYQTH LHSKSAGAV HNFLDTNLD 
      QIAKVRQSL EALSTLLIP NNRQKEASS RDSSS
    CDS sequence
    • >TC030578 RA
      atgttgtgt ttcgtgcaa ggattcgag gatagagac 
      gatggcaaa gagcgcaag ctgcaagtg gtcaagaat 
      cgcttcttg aaaaaatca cggtcgtct ggcaatttt 
      gggagcgcg ggggcccgg tcggctccc ggaacccca 
      caagggaca ctcggtact tccccggaa cctgtctcg 
      cagccatgc agtccgggc tgcacatcg ccctcgatc 
      cagcgaagt ccaagtcac tcgctcttc actaaatgc 
      gataaagcg tctttgaaa cacttatcg acgtcgacg 
      ccggccatg acggcccaa gcgcagctc accgaaccc 
      cccagcccc aaaacggca aaggagaac aaagtgcct 
      acgacgtcg aaagcgcgc cagaaaaaa ttcaacaga 
      cactttccg gctgttgac gatgacgag aaagtccta 
      aatcattat tcctgtgcc cttatcggt gatattctg 
      ctacaaggc cacttgtac atcaccaaa aactacttt 
      gcgttctat tccaacgtc ttcggatac gtgaccaag 
      cttctcatt ccaatgctg tccgtggag aaaataacg 
      aaagaaaaa accgcaagg ataatcccc aacgctgtt 
      ggaatcgcc acaagcgag gacaagcac gttttcggc 
      agcctaatg tcacgtgac agcacctac cgttacatg 
      gtcaaagtc tgggaggtg gcccagaac gcctctctc 
      ctcgtcgtc gagccggaa attgtggaa cctgtagtg 
      tcggacagc gacagcacg gagacgcgc gaaggcgaa 
      agcggccga gagtcgcct cttcccact ccccgattt 
      gctcagatg gtcctcaag gagcacaag gacaccgct 
      gccacaggg cctaaggtc gaaaaccaa caacgctca 
      atggaactg ctctacgct ctcaagcag gccatctcg 
      aaatttgcc agcctaccg cgacagagc ctaatactg 
      ttcgccacc acgttgctc ctcattctc ctcttctta 
      tcggctgct gtgctactt tatcgaatt agtaaaata 
      caaagcaaa tattcgttc aatttgcat gagacgatg 
      ctcgctgcg ggcagcgaa gacgtctac agcaatctg 
      ctgcaatat cagacgcac ttgcattcg aagtcggcg 
      ggggctgtg cacaacttc ttagacacg aatttagac 
      caaattgcg aaggtgaga cagtccctg gaagcgcta 
      tcgaccctg ttgatcccc aacaacagg cagaaagaa 
      gcgagttcc agagacagc agcagttag
    mRNA sequence
    • >TC030578 RA
      ctgccctcc acaacaacc gcttgacga cgtgtataa 
      taccgttcg ttcgactcg caacctcac caagcgtga 
      acacattca gtcaatgaa gagaccgcg aaaggggca 
      acgttttcg cctgccatg tgcccaaaa agtcgctaa 
      agtcgcctc tgaatcact taaaaaaaa tcgccacca 
      tgtcgccaa atcctgagt gatgttgtg tttcgtgca 
      aggattcga ggatagaga cgatggcaa agagcgcaa 
      gctgcaagt ggtcaagaa tcgcttctt gaaaaaatc 
      acggtcgtc tggcaattt tgggagcgc gggggcccg 
      gtcggctcc cggaacccc acaagggac actcggtac 
      ttccccgga acctgtctc gcagccatg cagtccggg 
      ctgcacatc gccctcgat ccagcgaag tccaagtca 
      ctcgctctt cactaaatg cgataaagc gtctttgaa 
      acacttatc gacgtcgac gccggccat gacggccca 
      agcgcagct caccgaacc ccccagccc caaaacggc 
      aaaggagaa caaagtgcc tacgacgtc gaaagcgcg 
      ccagaaaaa attcaacag acactttcc ggctgttga 
      cgatgacga gaaagtcct aaatcatta ttcctgtgc 
      ccttatcgg tgatattct gctacaagg ccacttgta 
      catcaccaa aaactactt tgcgttcta ttccaacgt 
      cttcggata cgtgaccaa gcttctcat tccaatgct 
      gtccgtgga gaaaataac gaaagaaaa aaccgcaag 
      gataatccc caacgctgt tggaatcgc cacaagcga 
      ggacaagca cgttttcgg cagcctaat gtcacgtga 
      cagcaccta ccgttacat ggtcaaagt ctgggaggt 
      ggcccagaa cgcctctct cctcgtcgt cgagccgga 
      aattgtgga acctgtagt gtcggacag cgacagcac 
      ggagacgcg cgaaggcga aagcggccg agagtcgcc 
      tcttcccac tccccgatt tgctcagat ggtcctcaa 
      ggagcacaa ggacaccgc tgccacagg gcctaaggt 
      cgaaaacca acaacgctc aatggaact gctctacgc 
      tctcaagca ggccatctc gaaatttgc cagcctacc 
      gcgacagag cctaatact gttcgccac cacgttgct 
      cctcattct cctcttctt atcggctgc tgtgctact 
      ttatcgaat tagtaaaat acaaagcaa atattcgtt 
      caatttgca tgagacgat gctcgctgc gggcagcga 
      agacgtcta cagcaatct gctgcaata tcagacgca 
      cttgcattc gaagtcggc gggggctgt gcacaactt 
      cttagacac gaatttaga ccaaattgc gaaggtgag 
      acagtccct ggaagcgct atcgaccct gttgatccc 
      caacaacag gcagaaaga agcgagttc cagagacag 
      cagcagtta gccacgtta ataacagtg ctaggaatg 
      tgatcagta ttcaatctt ttaaattta gatttagca 
      atatttcac ttaaatatt gttgccacc atcccagta 
      tcgacacta ggccgcgct gtttcatca tatttgcca 
      aatctatta ttgccataa gggtaatgg aactactcc 
      tatgtatag tccgcacgt ttatactat aagctcaat 
      ttgtcacga ggggttttt ggtgtaatg gaccgatga 
      ttgtaaact gggacttgt ttttaatag ttaattaat 
      ttatcgcca tctagtaca aacttattt tataattcc 
      actttagtt caacgcgcc caacactgt ctagcaata 
      atatcaaag gatttttta aaaaaaaag attgggaac 
      taatgctgt gaattggta ctgagcgat tttcgtaat 
      tttagaaac ttcgtaatc ctgattagg cagaaactt 
      gttgaaacg ttcatcctt tatttcaat gtacagttt 
      aataggaac gatttacac tgtttttaa ttttacatg 
      tacctacga tgtttatat gttaggtgt taatttatt 
      gcggttaga gttgtgttt tgtcaattg ttttgtaac 
      atgttaaca tagaagaaa cctgtgata tcttatttg 
      ttttgcttt tatcaataa atagttaca atttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY