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Gene information
General information
TC030577 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid38: 1381137..1397216
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030577
    iBeetle screen iB_02591
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Fertility
    egg chamber not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 4 (22 dpi)
    Days post injection


    Pupal morphology
    oblique dorsal thoracic muscles median sometimessplit - Number of animals showing the phenotype: 4

    Fertility
    egg production: number no/almost no eggs -
    ovary size decreased - Number of animals showing the phenotype: 4
    ovariole not present - Number of animals showing the phenotype: 4

    Comment: Just 2 or 3 epithelial pockets are visible where one would expect the half ovaries. No terminal filaments or any other ovariole-specific structures are visible. No signs of true lateral oviducts are detectable.
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030577 RA
      MNDLPEALN FTGRSMHSA HIMSTDLLS NVQKIKSEP 
      GIGNYTLSS LTSTLPLHN HALAPTSTF SPLTGENAN 
      GPAFTGNGN SNTSVESIE EPVRTASTS SSHSPQPSS 
      SATSTSNTT STNLSKPPY SYVALIAMA IQSSHSKRA 
      TLSEIYAYI TAKFPYFER NKKGWQNSI RHNLSLNEC 
      FVKVPREGG GERKGNYWT LDPQYEDMF ENGNYRRRR 
      RMKRPYRSA QPYPKAFFG DTLGQHGLP LASRSIFTP 
      PTYPPPYSR YDAASWLGP SQLASYTTC NSGGYSQQG 
      YPSPGFSSC GVRQEAAAR YPPYWPPDM VTTVKEEPS 
      SPTYRKCFI
    • >TC030577 RB
      MNDLPEALN FTGRSMHSA HIMSTDLLS NVQKIKSEP 
      GIGNYTLSS LTSTLPLHN HALAPTSTF SPLTGENAN 
      GPAFTGNGN SNTSVESIE EPVRTASTS SSHSPQPSS 
      SATSTSNTT STNLSKPPY SYVALIAMA IQSSHSKRA 
      TLSEIYAYI TAKFPYFER NKKGWQNSI RHNLSLNEC 
      FVKVPREGG GERKGNYWT LDPQYEDMF ENGNYRRRR 
      RMKRPYRSA QPYPKAFFG DTLGQHGLP LASRSIFTP 
      PTYPPPYSR YDAASWLGP SQLASYTTC NSGGYSQQG 
      YPSPGFSSC GVRQEAAAR YPPYWPPDS EYPPGPHSS 
      PPADQQLLL QW
    CDS sequence
    • >TC030577 RA
      atgaacgac ttgcccgaa gctctcaat tttacgggg 
      agatccatg cattcggcc cacattatg tccacagac 
      ttgttatca aatgttcag aagataaaa tcagagcct 
      ggtatcgga aactacaca ctgtcttct ctgacttcc 
      acgttacct cttcacaat cacgccctc gccccgacg 
      tcgactttt agccccctg acaggcgaa aatgcaaac 
      ggcccggcg ttcaccggc aacggaaac agcaatacc 
      tccgtagag tccatcgaa gaacccgtc agaacggcc 
      agtactagc tccagccac tcgccgcaa ccgtcttca 
      tcggcgact agcacttcc aataccact agcaccaac 
      ctaagcaaa cctccctat tcatatgta gccttaata 
      gcgatggcc atccagtcc tcccactcg aagcgcgcg 
      accctctct gagatctac gcttacatc accgcgaaa 
      ttcccctat ttcgagcgc aacaagaag gggtggcag 
      aactcgatc cggcacaac ctgagcttg aacgagtgc 
      ttcgtgaag gtgccccgc gaagggggc ggcgagcgc 
      aagggcaac tactggacg ctggacccc cagtacgag 
      gacatgttc gaaaacggg aactaccgc cggcggcgg 
      cggatgaag cgcccgtac cgcagcgcc cagccgtac 
      cccaaggcc ttcttcggg gacactctg gggcagcac 
      gggctgccc ctggccagc aggagcatc ttcacgccg 
      ccgacatac ccgcccccg tactcgcgc tacgacgcc 
      gcctcgtgg ctgggcccg tcgcagttg gcctcgtac 
      accacctgc aacagcggg ggctactcg cagcaagga 
      tacccgagt cccgggttt agttcttgc ggtgtgagg 
      caggaggcc gcggcgagg tacccgccc tactggccc 
      cccgatatg gtgactaca gtgaaggag gagccgagc 
      agtcctacc taccggaag tgcttcatt tga
    • >TC030577 RB
      atgaacgac ttgcccgaa gctctcaat tttacgggg 
      agatccatg cattcggcc cacattatg tccacagac 
      ttgttatca aatgttcag aagataaaa tcagagcct 
      ggtatcgga aactacaca ctgtcttct ctgacttcc 
      acgttacct cttcacaat cacgccctc gccccgacg 
      tcgactttt agccccctg acaggcgaa aatgcaaac 
      ggcccggcg ttcaccggc aacggaaac agcaatacc 
      tccgtagag tccatcgaa gaacccgtc agaacggcc 
      agtactagc tccagccac tcgccgcaa ccgtcttca 
      tcggcgact agcacttcc aataccact agcaccaac 
      ctaagcaaa cctccctat tcatatgta gccttaata 
      gcgatggcc atccagtcc tcccactcg aagcgcgcg 
      accctctct gagatctac gcttacatc accgcgaaa 
      ttcccctat ttcgagcgc aacaagaag gggtggcag 
      aactcgatc cggcacaac ctgagcttg aacgagtgc 
      ttcgtgaag gtgccccgc gaagggggc ggcgagcgc 
      aagggcaac tactggacg ctggacccc cagtacgag 
      gacatgttc gaaaacggg aactaccgc cggcggcgg 
      cggatgaag cgcccgtac cgcagcgcc cagccgtac 
      cccaaggcc ttcttcggg gacactctg gggcagcac 
      gggctgccc ctggccagc aggagcatc ttcacgccg 
      ccgacatac ccgcccccg tactcgcgc tacgacgcc 
      gcctcgtgg ctgggcccg tcgcagttg gcctcgtac 
      accacctgc aacagcggg ggctactcg cagcaagga 
      tacccgagt cccgggttt agttcttgc ggtgtgagg 
      caggaggcc gcggcgagg tacccgccc tactggccc 
      cccgatagt gagtacccc cccggcccc cacagttca 
      ccccctgct gaccaacag ctactgtta cagtggtga
    mRNA sequence
    • >TC030577 RA
      gatgtgaat gcagactta ttttaaagt ttttgatga 
      taatgtatg atgcgaaac gtatgcgtg agcaacatc 
      cgaaaaact tctcgtcgg tgaccgttt ttacgcact 
      gtttgtaca cagagcaat tgacagcaa aacgtgcta 
      gtttttagg tggatgtga ggtggaaat tcaaagttt 
      ggtggtgct ggttaagtg atccggatt tattttaca 
      aataacttc gttctgtag tgtttgttt gaaatacta 
      actgtaaat aaataaaca tagcgataa ttatgtttg 
      tgtgttggt gttgaactg tcttttgga cacgaattt 
      ttaaaaatg aacgacttg cccgaagct ctcaatttt 
      acggggaga tccatgcat tcggcccac attatgtcc 
      acagacttg ttatcaaat gttcagaag ataaaatca 
      gagcctggt atcggaaac tacacactg tcttctctg 
      acttccacg ttacctctt cacaatcac gccctcgcc 
      ccgacgtcg acttttagc cccctgaca ggcgaaaat 
      gcaaacggc ccggcgttc accggcaac ggaaacagc 
      aatacctcc gtagagtcc atcgaagaa cccgtcaga 
      acggccagt actagctcc agccactcg ccgcaaccg 
      tcttcatcg gcgactagc acttccaat accactagc 
      accaaccta agcaaacct ccctattca tatgtagcc 
      ttaatagcg atggccatc cagtcctcc cactcgaag 
      cgcgcgacc ctctctgag atctacgct tacatcacc 
      gcgaaattc ccctatttc gagcgcaac aagaagggg 
      tggcagaac tcgatccgg cacaacctg agcttgaac 
      gagtgcttc gtgaaggtg ccccgcgaa gggggcggc 
      gagcgcaag ggcaactac tggacgctg gacccccag 
      tacgaggac atgttcgaa aacgggaac taccgccgg 
      cggcggcgg atgaagcgc ccgtaccgc agcgcccag 
      ccgtacccc aaggccttc ttcggggac actctgggg 
      cagcacggg ctgcccctg gccagcagg agcatcttc 
      acgccgccg acatacccg cccccgtac tcgcgctac 
      gacgccgcc tcgtggctg ggcccgtcg cagttggcc 
      tcgtacacc acctgcaac agcgggggc tactcgcag 
      caaggatac ccgagtccc gggtttagt tcttgcggt 
      gtgaggcag gaggccgcg gcgaggtac ccgccctac 
      tggcccccc gatatggtg actacagtg aaggaggag 
      ccgagcagt cctacctac cggaagtgc ttcatttga 
      gatgacatc gtgattcga caacacatt tccaaggtt 
      ttttctctt ctactttct tattgaatt agttttttg 
      ttattcatg tggcttgaa gcactcatg cagaattca 
      ttgattttg tttatttat attccgtcc gtttcaagc 
      aatgggatc gacgcgatc cttgtttat atgtattta 
      tgtatgtaa ttaaattat gtataacaa tgatttttt 
      atctgtgaa ttttaaatt gtttttcat tgttaattt 
      tctttacat tttactaat gtaatagcg aaaaggttt 
      aaataaaat tttctcagt aac
    • >TC030577 RB
      gatgtgaat gcagactta ttttaaagt ttttgatga 
      taatgtatg atgcgaaac gtatgcgtg agcaacatc 
      cgaaaaact tctcgtcgg tgaccgttt ttacgcact 
      gtttgtaca cagagcaat tgacagcaa aacgtgcta 
      gtttttagg tggatgtga ggtggaaat tcaaagttt 
      ggtggtgct ggttaagtg atccggatt tattttaca 
      aataacttc gttctgtag tgtttgttt gaaatacta 
      actgtaaat aaataaaca tagcgataa ttatgtttg 
      tgtgttggt gttgaactg tcttttgga cacgaattt 
      ttaaaaatg aacgacttg cccgaagct ctcaatttt 
      acggggaga tccatgcat tcggcccac attatgtcc 
      acagacttg ttatcaaat gttcagaag ataaaatca 
      gagcctggt atcggaaac tacacactg tcttctctg 
      acttccacg ttacctctt cacaatcac gccctcgcc 
      ccgacgtcg acttttagc cccctgaca ggcgaaaat 
      gcaaacggc ccggcgttc accggcaac ggaaacagc 
      aatacctcc gtagagtcc atcgaagaa cccgtcaga 
      acggccagt actagctcc agccactcg ccgcaaccg 
      tcttcatcg gcgactagc acttccaat accactagc 
      accaaccta agcaaacct ccctattca tatgtagcc 
      ttaatagcg atggccatc cagtcctcc cactcgaag 
      cgcgcgacc ctctctgag atctacgct tacatcacc 
      gcgaaattc ccctatttc gagcgcaac aagaagggg 
      tggcagaac tcgatccgg cacaacctg agcttgaac 
      gagtgcttc gtgaaggtg ccccgcgaa gggggcggc 
      gagcgcaag ggcaactac tggacgctg gacccccag 
      tacgaggac atgttcgaa aacgggaac taccgccgg 
      cggcggcgg atgaagcgc ccgtaccgc agcgcccag 
      ccgtacccc aaggccttc ttcggggac actctgggg 
      cagcacggg ctgcccctg gccagcagg agcatcttc 
      acgccgccg acatacccg cccccgtac tcgcgctac 
      gacgccgcc tcgtggctg ggcccgtcg cagttggcc 
      tcgtacacc acctgcaac agcgggggc tactcgcag 
      caaggatac ccgagtccc gggtttagt tcttgcggt 
      gtgaggcag gaggccgcg gcgaggtac ccgccctac 
      tggcccccc gatagtgag taccccccc ggcccccac 
      agttcaccc cctgctgac caacagcta ctgttacag 
      tggtgacta cagtgaagg aggagccga gcagtccta 
      cctaccgga agtgcttca tttgagatg acatcgtga 
      ttcgacaac acatttcca aggtttttt ctcttctac 
      tttcttatt gaattagtt ttttgttat tcatgtggc 
      ttgaagcac tcatgcaga attcattga ttttgttta 
      tttatattc cgtccgttt caagcaatg ggatcgacg 
      cgatccttg tttatatgt atttatgta tgtaattaa 
      attatgtat aacaatgat tttttatct gtgaatttt 
      aaattgttt ttcattgtt aattttctt tacatttta 
      ctaatgtaa tagcgaaaa ggtttaaat aaaattttc 
      tcagtaac