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Gene information
General information
TC030576 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid37: 3664417..3670173
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030576
    iBeetle screen iB_02590
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    ovary without detectable defects - Number of animals showing the phenotype: 4
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030576 RA
      MAYRLSPLL RNFIKVGTV NCRTVSQHY PIDEHIFGL 
      TNEQIQLRE TVFNFAQKE LAPKANEID KKNGFSDLR 
      NFWKKLGDL GVLGITVNP EYGGTACSA IALSYGAHS 
      NLCVNQIHR NGTESQKQK YLPKLCSGE YMGALAMSE 
      PNSGSDVVS MKLRAEKKG DSYVLNGTK FWITNGPDA 
      DVLVVYAVT DPNAKKPQH GISAFIIEK GFPGFKAGP 
      KLDKLGMRG SNTSELIFE DCEVPKENL LGAENKGVY 
      VLMSGLDLE RLVLAGGPL GIMQACCDI TFSYAHVRK 
      QFQTRIGEF QLIQGKMAD MYTTIGACR SYLYNVAKA 
      CDNKKVNSK DCAGVILYC AEKATQMAL DAVQILGGN 
      GYINDYPTG RLLRDAKLY EIGAGTSEI RRLLIGRTL 
      NSEYS
    CDS sequence
    • >TC030576 RA
      atggcgtat cgattatca ccccttcta cgaaatttc 
      ataaaagtc ggtacagtg aactgtaga actgtctcc 
      caacattat cccatcgac gaacatatt tttggcctc 
      acaaacgag caaatccag cttcgagaa accgtattt 
      aattttgca cagaaagaa ttagcaccg aaggcgaat 
      gaaatcgat aaaaaaaat ggattcagt gacctgagg 
      aatttttgg aaaaaactt ggagatctc ggggtttta 
      ggaatcact gttaacccc gaatacggg ggcacagct 
      tgctctgcc atagccctc agttacggt gcccattcc 
      aatctctgt gtcaaccaa atccatcgc aacggcaca 
      gaatcccaa aaacaaaaa tacctcccg aaactttgt 
      tccggggag tacatgggc gccttggct atgtccgag 
      cccaactcc ggctccgat gtcgtctcc atgaagctc 
      cgtgctgaa aaaaagggc gattcctac gtcctcaac 
      ggcaccaag ttctggatc acgaacgga ccagatgcc 
      gacgttcta gttgtctat gccgtgact gaccccaac 
      gccaaaaaa ccccaacac ggcatttcg gctttcatc 
      atagaaaag ggattccca gggttcaaa gccggcccc 
      aaattggac aaattggga atgaggggc tcaaacacc 
      agcgaactg attttcgaa gactgtgaa gtgccaaag 
      gagaattta ttgggtgcg gaaaataaa ggggtttat 
      gttttgatg agtggatta gatttggaa aggctggtc 
      ttggccgga gggcccttg gggatcatg caagcctgc 
      tgtgatatt actttttcg tatgcgcat gtaaggaag 
      caatttcag acaaggatt ggcgaattc cagctcata 
      cagggtaaa atggccgac atgtatacc acaatcgga 
      gcttgtcga agctacttg tacaacgta gccaaagct 
      tgcgacaac aaaaaagta aatagcaaa gactgcgcc 
      ggagtaatt ttatactgt gctgagaaa gcgactcaa 
      atggctttg gatgcagtc caaatatta ggtggcaac 
      ggttacatc aatgattac ccaactgga agacttctc 
      cgcgatgcc aaactgtac gaaatcggc gctggaaca 
      tccgaaatc cgaagactg ctcattgga cgaacgctc 
      aattctgaa tactcatga
    mRNA sequence
    • >TC030576 RA
      ttcaacaca ctcattcag tagggtatc ttatagaaa 
      acagtgcat aatggcgta tcgattatc accccttct 
      acgaaattt cataaaagt cggtacagt gaactgtag 
      aactgtctc ccaacatta tcccatcga cgaacatat 
      ttttggcct cacaaacga gcaaatcca gcttcgaga 
      aaccgtatt taattttgc acagaaaga attagcacc 
      gaaggcgaa tgaaatcga taaaaaaaa tggattcag 
      tgacctgag gaatttttg gaaaaaact tggagatct 
      cggggtttt aggaatcac tgttaaccc cgaatacgg 
      gggcacagc ttgctctgc catagccct cagttacgg 
      tgcccattc caatctctg tgtcaacca aatccatcg 
      caacggcac agaatccca aaaacaaaa atacctccc 
      gaaactttg ttccgggga gtacatggg cgccttggc 
      tatgtccga gcccaactc cggctccga tgtcgtctc 
      catgaagct ccgtgctga aaaaaaggg cgattccta 
      cgtcctcaa cggcaccaa gttctggat cacgaacgg 
      accagatgc cgacgttct agttgtcta tgccgtgac 
      tgaccccaa cgccaaaaa accccaaca cggcatttc 
      ggctttcat catagaaaa gggattccc agggttcaa 
      agccggccc caaattgga caaattggg aatgagggg 
      ctcaaacac cagcgaact gattttcga agactgtga 
      agtgccaaa ggagaattt attgggtgc ggaaaataa 
      aggggttta tgttttgat gagtggatt agatttgga 
      aaggctggt cttggccgg agggccctt ggggatcat 
      gcaagcctg ctgtgatat tactttttc gtatgcgca 
      tgtaaggaa gcaatttca gacaaggat tggcgaatt 
      ccagctcat acagggtaa aatggccga catgtatac 
      cacaatcgg agcttgtcg aagctactt gtacaacgt 
      agccaaagc ttgcgacaa caaaaaagt aaatagcaa 
      agactgcgc cggagtaat tttatactg tgctgagaa 
      agcgactca aatggcttt ggatgcagt ccaaatatt 
      aggtggcaa cggttacat caatgatta cccaactgg 
      aagacttct ccgcgatgc caaactgta cgaaatcgg 
      cgctggaac atccgaaat ccgaagact gctcattgg 
      acgaacgct caattctga atactcatg aaaatttgt 
      ttaaataaa ggttttatt tggaa