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Gene information
General information
TC034740 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 4420739..4439926
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034740
    iBeetle screen iB_02588

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 1

    ovary without detectable defects - Number of animals showing the phenotype: 2
    vitellogenic egg chamber number decreased - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larval musculature partially degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): more than 80%

    larva cuticle mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection



    eclosion not fulfilled (not completely eclosed) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034740 RA
      MARRSPSRV GLALALICL ISHGQAEET CRTGCRGAA 
      QTFKYKEGT TYKYNYDSK IEVSLSGAD GQKSVTEVK 
      ATVLLTQQP QCNQILRLQ NVQIVGNDG KKHTNLPDT 
      DKPISINYH DGHLEDSIC ITPGDSQNS LNLKRAIAS 
      ILQANLKSG HETDVFGLC PTEVTHHKE GNMVVVQKA 
      RNLNRCAFR ENIKQDFIA TAFNLNSEI KSSPILNGD 
      YDATLRIKN GILDQATVH EHYLYVPFS VGTNGAKAA 
      IETNLKYVG TAKDNSQVK GGHPRSIIF EDPHPVHSP 
      HTNVNSILN AVKQTAKTI DVVVGEHTA KEFTNLVKI 
      LRVSKKDDL LAVYNQVRA GVGFPDKVA GKKIFLDAL 
      LRAGNGDNI EVAIELLKN KELTPVEQN LVFLGLSTV 
      RHPTENSLA TAAALLDQP NLPRNAYLG VGNLAGRFC 
      RQHRCEHAE GLKKLTQKL VQKLGKQPT NRHDENDLI 
      YVLKALGNF HHLCDEVLE KVTGLAQNR KNSNRVRVT 
      ALETYLADP CKDKLRDSA IGILKDVQQ DSEVRIKAY 
      LALAQCPNS KVASAVKAV LEKEPSYQV GGFIQTHIR 
      NIRASANPD KELAKQHLG QITVPKKYP FDLRKYSAN 
      GEFSYTFDT LGIANSIES NIIYSQTSF LPRSTSLNL 
      TAEIFGHTL NFLDIQTRQ ENLDRLVEH YLGPKGLLR 
      SKTLQQLWD GTGPRVKKL TEELDSRLR ELLRARRDL 
      SKAEIESVG KQVEIKTNE LNRDLDLDL SVKAFGSEV 
      LFLNLNDDV RKYTPEAVI DQILAGFKQ GLDRIKHFD 
      ETLRSNLLF LDAELSYPT SSGFPLRLG VEGSANLQV 
      KTAGSFDMK QGHKVKFTL IPSANVEVS GRLTVDTLV 
      LESGLKIIS TLHTATGGD LTYEALKNG EDIKFSFPV 
      EKQNILSAS HDIVFHNRE WGSPEVNTP LKFAQNKDF 
      SICLDQLSP FIGLTFCGE FNGPNLQGQ KVPILPFPL 
      SGDARVEVT VEKEDLSMY HFRNEYFLQ ESGKTGLEV 
      VLEALGKSG QKKVALTLE AFLQPERYF KAALTSPVT 
      SAVLEGRIT LTDSERSLS LRFAQDKNE YFGKIGVAV 
      QGSAAKAIY KPLVQYKTP QDGALQVPP YRVDGQVIV 
      ENNGPATTY TLDNVKLII PNQKTITFN GKFGHEPET 
      VFADLTVSD GNSNAAFKG KLRVNANLI KYNAELKNS 
      LNPSANIHL KGELAKSAE ELKNFFQVA HGGDFSDKN 
      SILTLENSL KHRNKGPQD FLLDTKNKL SYPGLGANL 
      EFEFQLKPK AVEYDLDVQ YGSTKFGSE LEYEHNSKR 
      EGTEFKLDF GIWGLNNRV NIKGKRTIN GEQSHIENS 
      YEINGKRTE INGKIRHHL RPQDVDFGA DLEIKVPQH 
      NTPFKVLGE FKLNPNEID ASNKITSGG TVYLDAFLK 
      ANKAGNANG SIKVNLKGQ LLVSGQVKA NKGSGTGDL 
      EVELPSQGR KSKIDTTFT VHEPTYNIV VNIYPVFDK 
      DKSKKITLS TQTSLKPHQ LDSKNFIDF FGNKVEGNV 
      KGSQQGDLS NGKITGEAE LTLPNNCYF AGKIDREMK 
      TVEQVLNGN AIASIEYRP NKNQPGNKF TIKGHAKDT 
      NPSEKIFDL NYQITAESA KGKNLNFDL MVKRVPQGE 
      RFNIQMHNK IYGSELPSP IETTLKGTC DGFTGSFEG 
      ASTYAPFGK IAIRGKHDF SGSGKPVSG ELDLDVTTT 
      SQHLKTMKF SIGGSLHKS DNVDVKGQV KFFGENAKG 
      VFAEFDSNG EVKVSDREA RARGTIKTG QLDPISVSV 
      GMSRLEDKE VKGDLGFEF GKGQKAKGD FTLIRLQPH 
      EYKLDLKFD TPAEKFKSN RIQVHTKSN DARTHHQSE 
      VILTSDGKT WTANTDLVL SEIAPSVDI KVKCPEGKL 
      RQFLAKVTK MSDRQLGAQ LKIVNQKEN FLLEGTVDA 
      NVENIDDFH VKITANAPT LKIDKYVIE AQNKPAKTG 
      RRIQVTAKS GNKNILAGS TSYTAREEH GKVIIEGSG 
      SFKLHDETK SANFKYIRQ NLVQAKNGE TGIEISFDA 
      SLGNKAIDA ELKITDKQF RILNSYCEE KKQCAHVEV 
      DVKTTVDDV KAFNQEIEI AIDLRKLGL SHEFGLKAV 
      TTRKEYVLD HTVDMHFQS QENSKYQYS LYVHPNQAG 
      VSLTTPKRI IALESKINV PSKFLQDGG SVSGEVFFY 
      TDKKNQPNK KSGLISYLN LNPKGPTLD GEIKFTNPA 
      LKRPLLISI KSKYVPMSL ESVVTFDVF AQPDQKLVI 
      KHTEKWSDP KDYKKMEAK EVVEIKSTG LHIDIHYEE 
      YAFASVEDH HYKAEVKLR YSGPGSKHE SGALYEVTP 
      KNLEAGIKL FNHQLFHLT SKYQLSQNH QVFDSELGC 
      YGHRPLVSH LEVKNLNTL KYSIACKDK PNDKLLVNC 
      GFKPGEIAD VRAEIIKGG QTHELGHAT VKLDDANFL 
      KSDYKVQAK NIEELLFKP IKDGIQREG DELAKLSSE 
      MMQHGQKEL QSFGEVAKR ATPDIAPLR QYYTGELAK 
      IKDEILADK TIKDLSEFL YNILSVFTQ TFGDSFKHF 
      SEFIENIYI SFDKTFGNI LQIIEKDIL PHAKVTCDK 
      FLKLFLHAV DIATHAVLT VGVQIADIA EKYQPEFQQ 
      LANVFGQVS QDVLRASLN LYEAVKSVI IDQWQQIYN 
      EIKAMPAVE QLKAEYEQY VKHGLPARE NIANAIKDL 
      LSTIKDLAP TEELKAFIQ AISDYVCPK LNNQKIDDK 
      VAIDRIIQS GIAGGKSIM AIVTGSRPV PELHNFQLP 
      LPATLLNRL PKLAAFRFS PFSFILGEE LYVSEFFLS 
      LVNRPRNWI PPFPRKLQ
    CDS sequence
    • >TC034740 RA
      atggctcga cgaagcccc tctagagtg ggcctagcc 
      ctggcactt atctgcctc ataagccac ggacaggcc 
      gaagagact tgccggaca ggatgtcgc ggagctgct 
      caaactttc aaatacaag gaaggcacc acgtacaag 
      tacaactac gacagcaaa atcgaagtg agtctttcg 
      ggggccgac ggccagaag agcgtcacc gaagtcaag 
      gccacagta ttacttacc caacagccc caatgcaac 
      caaatcttg cgcctccaa aacgtccaa atcgtgggc 
      aacgatggc aaaaaacac accaacctc cccgacacc 
      gacaaaccc atcagtatc aactaccac gacggccac 
      ctggaggac agcatctgc atcacacct ggtgacagc 
      cagaactcg ctcaatctc aagcgcgcc atcgcgtcc 
      atcctccag gcgaacctg aagtcgggc cacgagacc 
      gacgtgttc gggctctgc cccacggag gtgacccac 
      cacaaggag ggcaacatg gtcgtggtg cagaaggcg 
      cgcaacctg aaccggtgc gccttccgc gagaacatc 
      aagcaggac ttcatcgcg acggcgttc aatctcaac 
      agcgagatt aaatcgagt ccgatttta aacggggat 
      tatgacgcc acgctccgc atcaaaaac gggatcctg 
      gaccaggcc accgtccac gaacactac ctctacgtc 
      cccttctcc gtgggcacg aacggggcc aaagccgcg 
      atcgaaacc aacctgaaa tacgtcggg acggcgaaa 
      gacaactcg caggttaaa ggcgggcat cctaggtcc 
      atcatcttc gaggacccg cacccggtg cattcgccg 
      cacaccaac gtcaacagc atcctcaac gctgtcaaa 
      caaaccgcc aagacgatt gatgtcgtc gtgggcgaa 
      cacacagcc aaggagttt accaacttg gtgaaaata 
      ctacgtgtt agtaaaaaa gatgatctt ttggccgtc 
      tacaaccag gtgcgcgct ggcgtggga ttccccgac 
      aaagtcgcc ggtaagaaa atcttcctg gacgccctc 
      ctcagagcc ggtaacggc gacaacatc gaagtcgcc 
      atcgaactg ctcaaaaac aaagaactg accccagtt 
      gaacaaaac ttggttttc ttgggatta tcgacagtg 
      cgtcacccg actgaaaat tcccttgcg acagctgcg 
      gcccttcta gaccaaccc aaccttcct cgaaacgcc 
      tatctagga gttggaaac ctagccgga aggttttgc 
      cgccaacat cggtgcgaa cacgccgaa ggacttaaa 
      aaattgacg cagaaattg gttcagaag ttgggcaaa 
      cagccgacg aatcgacac gatgagaac gatttgatc 
      tatgtgttg aaggcgttg ggtaacttc catcatttg 
      tgcgatgag gttttggag aaggttacc ggtttggca 
      cagaatagg aaaaattcg aatagggtg agggttaca 
      gcgttggag acgtatctg gctgatcct tgcaaggat 
      aagttgagg gatagtgcg attggtatc ctgaaggat 
      gttcaacag gattccgaa gttaggatt aaggcctat 
      ctagcactt gcacaatgt ccgaacagt aaggtggca 
      agtgccgtt aaggccgtt ttggagaag gaacccagt 
      tatcaagtt ggtggtttc atccaaacc cacatccgc 
      aacatccgc gcctcagcc aaccccgac aaagaactc 
      gccaaacaa cacttgggc caaatcacc gttcccaaa 
      aaatacccc ttcgacctt cgcaaatac tcagcaaac 
      ggcgaattc tcctacact ttcgacact ttaggaatc 
      gccaattcg atcgaaagc aacatcatc tactcgcaa 
      acctctttc ctaccacgg tccactagc ctaaacctg 
      actgccgaa attttcgga cacacctta aacttcctg 
      gacattcaa acgcgtcaa gagaacttg gaccgcttg 
      gttgaacac tacttgggc cccaaagga ctgctccgc 
      tccaaaacg ttgcaacaa ctctgggat ggaactgga 
      ccacgtgtc aaaaaactg accgaggag ttggacagc 
      aggttgagg gagttgttg agagcacgt cgcgattta 
      tcaaaggcc gagattgaa tcggtgggg aaacaagtc 
      gagattaag accaacgaa ctgaatagg gacctggac 
      ttggatttg agcgtcaaa gcgttcggt tccgaagtt 
      ttgttcttg aatttgaac gacgatgtt cggaaatac 
      actcccgag gctgtgatt gatcagatt ttggctggg 
      tttaagcaa gggcttgat agaatcaaa cacttcgac 
      gaaaccctg cgaagtaac cttttgttc ctcgacgcg 
      gaactttcc taccccacg tcgtctggg tttcccctt 
      cggctggga gtcgaaggt tcggccaat ctccaggtg 
      aagacggcc ggttctttc gatatgaaa caaggccac 
      aaagtcaaa ttcactcta attccaagc gcgaatgtt 
      gaagtttcc gggcggttg actgtcgat actttggtt 
      cttgaaagt gggcttaag atcatttcc actttgcat 
      acggccact ggcggtgat ctcacgtac gaagcgttg 
      aaaaacggt gaagatatc aaattttcg ttccccgtt 
      gagaaacag aatatactt tcggctagt catgatatt 
      gttttccat aatcgtgaa tggggaagt cctgaagtt 
      aatactcct ttgaagttt gcgcaaaat aaagatttc 
      tcgatctgt ttggatcaa ttgagtccg tttatcggc 
      ttgacgttc tgtggggaa ttcaatgga cctaatcta 
      caaggacag aaggtgccg attttgccg ttccccttg 
      tctggggat gctagagtt gaagtgacc gttgaaaag 
      gaggacttg tccatgtac catttccgc aatgaatac 
      ttcttgcaa gagagtggt aaaaccggc cttgaagtc 
      gtactcgaa gccttggga aagagcggc caaaagaag 
      gtcgcccta acacttgaa gccttcctc caaccggag 
      cgttacttc aaggctgct ttaacctct cccgttact 
      tcagcagtc cttgaaggt cgtataacc ttaaccgac 
      agcgaaaga tccctttcg ttgcgtttt gcccaagac 
      aagaacgaa tatttcgga aaaattgga gtcgcagtc 
      caaggctcg gccgcaaaa gccatctac aagccacta 
      gtccaatac aaaacaccc caagacgga gcccttcaa 
      gtaccacca taccgagtc gatggccaa gtaatcgtc 
      gaaaacaac ggcccagca accacttac accctcgac 
      aacgtcaag ttaataatc cccaaccag aaaaccatc 
      accttcaat ggaaaattc ggccacgaa cccgaaacg 
      gttttcgcc gacttgacc gtctcggac ggcaacagc 
      aacgcagcc ttcaagggc aaactccgc gtcaacgcc 
      aaccttatc aagtacaac gccgaactc aaaaactcc 
      ctaaacccg agcgcaaac atccacctt aaaggcgaa 
      ctcgcaaaa tcggccgaa gagttgaaa aacttcttc 
      caagtggcg cacggtggc gacttcagc gacaaaaac 
      agcatcctc actttggag aactcgctc aaacatcgc 
      aacaaaggc ccgcaggac ttcctactc gacaccaaa 
      aacaaactc tcgtatcct ggccttggg gccaacctc 
      gagttcgaa ttccaactc aaaccaaag gctgtggag 
      tacgacctt gacgtccag tacggcagt accaagttc 
      ggttccgaa ctggaatac gagcacaac tccaagcgc 
      gaaggcacc gaattcaag ctagacttc ggtatttgg 
      ggcctcaac aaccgggtc aatatcaag gggaagaga 
      acaataaac ggggaacaa tcccacatt gaaaactcg 
      tacgaaatc aacgggaaa cgcaccgaa atcaacgga 
      aaaatcagg caccacttg cggccgcag gacgttgat 
      tttggggcc gatctggag atcaaagta ccacaacat 
      aacaccccg ttcaaggtt ctgggcgag tttaaactc 
      aacccgaac gaaatcgac gcttccaat aaaatcact 
      tcgggtggt accgtctat cttgacgcg ttcctcaag 
      gccaacaag gccgggaac gccaatgga agcatcaaa 
      gttaacttg aaaggacaa ttgctggtg agtggtcaa 
      gtcaaggcg aacaaaggc agcggaacg ggcgatcta 
      gaagtcgag ttaccctcg caaggacgc aagagcaaa 
      atcgataca acctttaca gtgcacgaa cccacctac 
      aacattgtt gtcaacatc taccccgtc ttcgacaag 
      gacaagtcg aagaaaatc acactttcg acacaaact 
      agcctcaaa ccgcaccaa ctcgactcg aagaacttc 
      atcgacttc ttcgggaac aaagtcgaa gggaatgtg 
      aaagggtcg caacagggg gacctaagt aatgggaaa 
      atcaccggc gaagccgaa cttaccctc cctaataat 
      tgctacttc gcggggaaa atcgaccgg gagatgaag 
      accgtcgaa caagttctt aacggaaat gccatcgcc 
      agtatagag taccgacca aacaaaaac caaccaggg 
      aataaattc accatcaaa gggcatgcc aaagatacg 
      aaccccagc gagaagatc ttcgatctg aattaccaa 
      attacggcc gaatctgcc aaaggcaag aacctgaac 
      ttcgatctt atggtcaag agagtacca caaggcgaa 
      cggttcaac atccaaatg cacaacaag atctacggt 
      tcggaacta ccaagtccc atcgaaacc acgttgaag 
      ggcacgtgt gatgggttc acgggttcg ttcgaaggc 
      gcctccact tacgcaccc ttcgggaaa atcgcaatt 
      aggggcaaa cacgacttt agtggttct gggaaacca 
      gtcagtggc gaactcgat ttagacgtg accacgacc 
      agccaacac ttgaaaacc atgaagttt agcatcgga 
      ggttccctc cacaagagt gacaatgtt gacgtcaaa 
      ggtcaagtc aagttcttc ggcgagaac gcgaaagga 
      gtcttcgcc gagttcgac tcaaacggc gaagtcaaa 
      gtcagtgac cgagaggca agagctaga ggaaccatc 
      aagacgggc caactggac cccatctcc gtatcagtc 
      ggaatgagc cgtttggag gacaaagaa gtcaaaggt 
      gaccttggt ttcgaattc ggcaaaggc cagaaagcc 
      aagggcgac ttcacccta atccgcctc caaccgcac 
      gaatacaaa ctcgacctc aaattcgac accccagct 
      gagaaattc aaaagcaac cgaatccaa gtgcacacc 
      aagagcaac gacgcccgc acccaccac caaagcgaa 
      gtgatcctg acctcggac ggcaagacc tggaccgcc 
      aacaccgac ctcgtcctc tccgaaatc gccccaagt 
      gtcgacatc aaagtcaag tgtcccgaa ggcaaactg 
      cgccagttc ctcgccaaa gtcaccaaa atgagcgac 
      cgccaactc ggcgcccaa ctcaagatc gtcaaccaa 
      aaagagaac ttcctcctc gaaggcact gtggacgcc 
      aacgtcgag aacatcgac gacttccac gtgaaaatc 
      acagcgaac gcgccgacc ttgaaaatc gacaagtac 
      gtcatcgaa gcacagaat aaacccgcc aagaccgga 
      cggcgaatc caagtgacg gcgaaatca ggcaacaag 
      aatatcctg gccgggagc acgagctac acagcgcgg 
      gaagagcac gggaaagtg atcatcgaa ggctcgggt 
      tcgtttaaa ctacacgac gaaacgaaa tcggcgaac 
      tttaaatac atccgccag aatctggtc caggccaag 
      aacggcgaa acgggcatc gagatctcc ttcgacgcc 
      agtttgggg aacaaagca attgatgca gaattgaaa 
      atcaccgac aaacagttc aggattttg aactcctac 
      tgcgaggaa aagaaacaa tgtgcacat gtggaagtc 
      gatgttaag acaaccgtt gacgatgtc aaagccttc 
      aaccaggaa atcgaaatc gcgattgac ttacgcaag 
      ctggggttg tcgcacgag tttggcctc aaagcggtg 
      accactcgc aaggagtac gtcctagac cacactgta 
      gatatgcat ttccaaagc caggagaac agcaagtac 
      caatacagt ctctacgtc caccccaac caggctgga 
      gttagccta acgaccccc aaacgaatc atcgccctt 
      gaaagtaaa atcaacgtc ccgtccaag ttcctccaa 
      gacggtggt tccgtctcc ggcgaagtc ttcttctac 
      acggacaag aagaaccaa ccaaacaaa aaatccgga 
      ctcatctcc tacctcaac ctcaacccc aaaggacca 
      acccttgac ggtgagatc aaattcaca aacccggcc 
      ctaaaacgc ccacttctg atcagcatc aagtcgaaa 
      tacgtgcca atgtcgctg gaaagcgtg gtcacgttt 
      gacgttttc gcccaaccg gaccagaag ctcgtcatc 
      aaacacacg gagaagtgg tccgacccg aaggactac 
      aagaagatg gaagccaaa gaagtggtg gaaattaag 
      agtacaggc ctccacatt gatatccac tacgaggag 
      tacgctttc gcgtcggtc gaagaccac cactacaag 
      gccgaagtc aagttgagg tactccgga cctgggtcc 
      aaacacgaa tccggcgcg ctttacgaa gtcaccccc 
      aagaatctc gaagccggg atcaaactc ttcaaccac 
      caattgttc cacctgacg tccaaatac caactctcg 
      caaaaccac caagtgttt gatagcgag ttgggttgc 
      tacggccat agaccgttg gtgagtcac ctggaggtg 
      aagaacctc aacactttg aagtactcg atcgcgtgc 
      aaagacaag ccaaacgac aagctcctg gttaattgt 
      ggcttcaag ccgggggag attgccgat gttagggcc 
      gaaatcatc aaaggcggg caaacgcac gagttgggg 
      cacgcgact gttaaacta gacgatgct aatttcctc 
      aagtccgac tacaaggtc caagccaag aacatcgag 
      gagttgctg ttcaagccg atcaaggac gggatccag 
      cgtgaaggg gacgagttg gcgaaactc tcgtcggag 
      atgatgcaa cacggccag aaggagttg cagagcttt 
      ggcgaagtc gccaaacgg gcaacgccc gacatcgcc 
      ccgcttcgc cagtactac accggcgaa ctcgccaaa 
      atcaaggac gagatcctg gccgacaaa accatcaag 
      gacttgagc gaattcctt tacaacatc ttgtcagtt 
      ttcacacaa actttcggc gattcgttc aaacacttt 
      agcgagttt attgagaat atttacatc agttttgat 
      aagactttc gggaacatc ctccaaatt atcgagaag 
      gatatttta ccgcacgcc aaagtcacg tgtgacaag 
      ttcctgaaa ctgttcctt cacgctgtt gacattgcc 
      acccatgcc gttttaaca gtcggtgtc caaattgcc 
      gacatcgcc gaaaaatac caacccgag ttccagcaa 
      cttgccaat gtttttgga caagtcagt caggacgtt 
      ttacgggca agtctcaac ctgtacgag gccgtaaaa 
      agtgtgatt atcgaccaa tggcaacaa atttacaac 
      gaaattaag gcaatgcca gctgttgaa caactcaaa 
      gccgagtat gaacaatac gttaaacat ggattaccg 
      gcccgcgaa aacattgcc aatgctatc aaagacttg 
      ctttcgacg attaaagat ttagcaccg actgaagaa 
      cttaaagct ttcattcaa gctattagt gactatgtt 
      tgtccgaaa ctcaacaac caaaagatt gacgataaa 
      gtggcaatt gatcgaatt attcaatcg ggaattgct 
      ggcggtaaa tcaatcatg gccattgtg accggaagt 
      cggccggtt ccagaattg cacaacttc caacttcca 
      cttcctgca acacttctt aaccgactt cccaaactt 
      gcagcgttt aggtttagt ccgttctcg ttcattttg 
      ggcgaggaa ttgtacgtt tctgagttc ttcctatcg 
      cttgtgaat agaccgagg aactggata ccaccattc 
      ccacgtaag ttacaataa
    mRNA sequence
    • >TC034740 RA
      cagttggag tgcgctatg gctcgacga agcccctct 
      agagtgggc ctagccctg gcacttatc tgcctcata 
      agccacgga caggccgaa gagacttgc cggacagga 
      tgtcgcgga gctgctcaa actttcaaa tacaaggaa 
      ggcaccacg tacaagtac aactacgac agcaaaatc 
      gaagtgagt ctttcgggg gccgacggc cagaagagc 
      gtcaccgaa gtcaaggcc acagtatta cttacccaa 
      cagccccaa tgcaaccaa atcttgcgc ctccaaaac 
      gtccaaatc gtgggcaac gatggcaaa aaacacacc 
      aacctcccc gacaccgac aaacccatc agtatcaac 
      taccacgac ggccacctg gaggacagc atctgcatc 
      acacctggt gacagccag aactcgctc aatctcaag 
      cgcgccatc gcgtccatc ctccaggcg aacctgaag 
      tcgggccac gagaccgac gtgttcggg ctctgcccc 
      acggaggtg acccaccac aaggagggc aacatggtc 
      gtggtgcag aaggcgcgc aacctgaac cggtgcgcc 
      ttccgcgag aacatcaag caggacttc atcgcgacg 
      gcgttcaat ctcaacagc gagattaaa tcgagtccg 
      attttaaac ggggattat gacgccacg ctccgcatc 
      aaaaacggg atcctggac caggccacc gtccacgaa 
      cactacctc tacgtcccc ttctccgtg ggcacgaac 
      ggggccaaa gccgcgatc gaaaccaac ctgaaatac 
      gtcgggacg gcgaaagac aactcgcag gttaaaggc 
      gggcatcct aggtccatc atcttcgag gacccgcac 
      ccggtgcat tcgccgcac accaacgtc aacagcatc 
      ctcaacgct gtcaaacaa accgccaag acgattgat 
      gtcgtcgtg ggcgaacac acagccaag gagtttacc 
      aacttggtg aaaatacta cgtgttagt aaaaaagat 
      gatcttttg gccgtctac aaccaggtg cgcgctggc 
      gtgggattc cccgacaaa gtcgccggt aagaaaatc 
      ttcctggac gccctcctc agagccggt aacggcgac 
      aacatcgaa gtcgccatc gaactgctc aaaaacaaa 
      gaactgacc ccagttgaa caaaacttg gttttcttg 
      ggattatcg acagtgcgt cacccgact gaaaattcc 
      cttgcgaca gctgcggcc cttctagac caacccaac 
      cttcctcga aacgcctat ctaggagtt ggaaaccta 
      gccggaagg ttttgccgc caacatcgg tgcgaacac 
      gccgaagga cttaaaaaa ttgacgcag aaattggtt 
      cagaagttg ggcaaacag ccgacgaat cgacacgat 
      gagaacgat ttgatctat gtgttgaag gcgttgggt 
      aacttccat catttgtgc gatgaggtt ttggagaag 
      gttaccggt ttggcacag aataggaaa aattcgaat 
      agggtgagg gttacagcg ttggagacg tatctggct 
      gatccttgc aaggataag ttgagggat agtgcgatt 
      ggtatcctg aaggatgtt caacaggat tccgaagtt 
      aggattaag gcctatcta gcacttgca caatgtccg 
      aacagtaag gtggcaagt gccgttaag gccgttttg 
      gagaaggaa cccagttat caagttggt ggtttcatc 
      caaacccac atccgcaac atccgcgcc tcagccaac 
      cccgacaaa gaactcgcc aaacaacac ttgggccaa 
      atcaccgtt cccaaaaaa taccccttc gaccttcgc 
      aaatactca gcaaacggc gaattctcc tacactttc 
      gacacttta ggaatcgcc aattcgatc gaaagcaac 
      atcatctac tcgcaaacc tctttccta ccacggtcc 
      actagccta aacctgact gccgaaatt ttcggacac 
      accttaaac ttcctggac attcaaacg cgtcaagag 
      aacttggac cgcttggtt gaacactac ttgggcccc 
      aaaggactg ctccgctcc aaaacgttg caacaactc 
      tgggatgga actggacca cgtgtcaaa aaactgacc 
      gaggagttg gacagcagg ttgagggag ttgttgaga 
      gcacgtcgc gatttatca aaggccgag attgaatcg 
      gtggggaaa caagtcgag attaagacc aacgaactg 
      aatagggac ctggacttg gatttgagc gtcaaagcg 
      ttcggttcc gaagttttg ttcttgaat ttgaacgac 
      gatgttcgg aaatacact cccgaggct gtgattgat 
      cagattttg gctgggttt aagcaaggg cttgataga 
      atcaaacac ttcgacgaa accctgcga agtaacctt 
      ttgttcctc gacgcggaa ctttcctac cccacgtcg 
      tctgggttt ccccttcgg ctgggagtc gaaggttcg 
      gccaatctc caggtgaag acggccggt tctttcgat 
      atgaaacaa ggccacaaa gtcaaattc actctaatt 
      ccaagcgcg aatgttgaa gtttccggg cggttgact 
      gtcgatact ttggttctt gaaagtggg cttaagatc 
      atttccact ttgcatacg gccactggc ggtgatctc 
      acgtacgaa gcgttgaaa aacggtgaa gatatcaaa 
      ttttcgttc cccgttgag aaacagaat atactttcg 
      gctagtcat gatattgtt ttccataat cgtgaatgg 
      ggaagtcct gaagttaat actcctttg aagtttgcg 
      caaaataaa gatttctcg atctgtttg gatcaattg 
      agtccgttt atcggcttg acgttctgt ggggaattc 
      aatggacct aatctacaa ggacagaag gtgccgatt 
      ttgccgttc cccttgtct ggggatgct agagttgaa 
      gtgaccgtt gaaaaggag gacttgtcc atgtaccat 
      ttccgcaat gaatacttc ttgcaagag agtggtaaa 
      accggcctt gaagtcgta ctcgaagcc ttgggaaag 
      agcggccaa aagaaggtc gccctaaca cttgaagcc 
      ttcctccaa ccggagcgt tacttcaag gctgcttta 
      acctctccc gttacttca gcagtcctt gaaggtcgt 
      ataacctta accgacagc gaaagatcc ctttcgttg 
      cgttttgcc caagacaag aacgaatat ttcggaaaa 
      attggagtc gcagtccaa ggctcggcc gcaaaagcc 
      atctacaag ccactagtc caatacaaa acaccccaa 
      gacggagcc cttcaagta ccaccatac cgagtcgat 
      ggccaagta atcgtcgaa aacaacggc ccagcaacc 
      acttacacc ctcgacaac gtcaagtta ataatcccc 
      aaccagaaa accatcacc ttcaatgga aaattcggc 
      cacgaaccc gaaacggtt ttcgccgac ttgaccgtc 
      tcggacggc aacagcaac gcagccttc aagggcaaa 
      ctccgcgtc aacgccaac cttatcaag tacaacgcc 
      gaactcaaa aactcccta aacccgagc gcaaacatc 
      caccttaaa ggcgaactc gcaaaatcg gccgaagag 
      ttgaaaaac ttcttccaa gtggcgcac ggtggcgac 
      ttcagcgac aaaaacagc atcctcact ttggagaac 
      tcgctcaaa catcgcaac aaaggcccg caggacttc 
      ctactcgac accaaaaac aaactctcg tatcctggc 
      cttggggcc aacctcgag ttcgaattc caactcaaa 
      ccaaaggct gtggagtac gaccttgac gtccagtac 
      ggcagtacc aagttcggt tccgaactg gaatacgag 
      cacaactcc aagcgcgaa ggcaccgaa ttcaagcta 
      gacttcggt atttggggc ctcaacaac cgggtcaat 
      atcaagggg aagagaaca ataaacggg gaacaatcc 
      cacattgaa aactcgtac gaaatcaac gggaaacgc 
      accgaaatc aacggaaaa atcaggcac cacttgcgg 
      ccgcaggac gttgatttt ggggccgat ctggagatc 
      aaagtacca caacataac accccgttc aaggttctg 
      ggcgagttt aaactcaac ccgaacgaa atcgacgct 
      tccaataaa atcacttcg ggtggtacc gtctatctt 
      gacgcgttc ctcaaggcc aacaaggcc gggaacgcc 
      aatggaagc atcaaagtt aacttgaaa ggacaattg 
      ctggtgagt ggtcaagtc aaggcgaac aaaggcagc 
      ggaacgggc gatctagaa gtcgagtta ccctcgcaa 
      ggacgcaag agcaaaatc gatacaacc tttacagtg 
      cacgaaccc acctacaac attgttgtc aacatctac 
      cccgtcttc gacaaggac aagtcgaag aaaatcaca 
      ctttcgaca caaactagc ctcaaaccg caccaactc 
      gactcgaag aacttcatc gacttcttc gggaacaaa 
      gtcgaaggg aatgtgaaa gggtcgcaa cagggggac 
      ctaagtaat gggaaaatc accggcgaa gccgaactt 
      accctccct aataattgc tacttcgcg gggaaaatc 
      gaccgggag atgaagacc gtcgaacaa gttcttaac 
      ggaaatgcc atcgccagt atagagtac cgaccaaac 
      aaaaaccaa ccagggaat aaattcacc atcaaaggg 
      catgccaaa gatacgaac cccagcgag aagatcttc 
      gatctgaat taccaaatt acggccgaa tctgccaaa 
      ggcaagaac ctgaacttc gatcttatg gtcaagaga 
      gtaccacaa ggcgaacgg ttcaacatc caaatgcac 
      aacaagatc tacggttcg gaactacca agtcccatc 
      gaaaccacg ttgaagggc acgtgtgat gggttcacg 
      ggttcgttc gaaggcgcc tccacttac gcacccttc 
      gggaaaatc gcaattagg ggcaaacac gactttagt 
      ggttctggg aaaccagtc agtggcgaa ctcgattta 
      gacgtgacc acgaccagc caacacttg aaaaccatg 
      aagtttagc atcggaggt tccctccac aagagtgac 
      aatgttgac gtcaaaggt caagtcaag ttcttcggc 
      gagaacgcg aaaggagtc ttcgccgag ttcgactca 
      aacggcgaa gtcaaagtc agtgaccga gaggcaaga 
      gctagagga accatcaag acgggccaa ctggacccc 
      atctccgta tcagtcgga atgagccgt ttggaggac 
      aaagaagtc aaaggtgac cttggtttc gaattcggc 
      aaaggccag aaagccaag ggcgacttc accctaatc 
      cgcctccaa ccgcacgaa tacaaactc gacctcaaa 
      ttcgacacc ccagctgag aaattcaaa agcaaccga 
      atccaagtg cacaccaag agcaacgac gcccgcacc 
      caccaccaa agcgaagtg atcctgacc tcggacggc 
      aagacctgg accgccaac accgacctc gtcctctcc 
      gaaatcgcc ccaagtgtc gacatcaaa gtcaagtgt 
      cccgaaggc aaactgcgc cagttcctc gccaaagtc 
      accaaaatg agcgaccgc caactcggc gcccaactc 
      aagatcgtc aaccaaaaa gagaacttc ctcctcgaa 
      ggcactgtg gacgccaac gtcgagaac atcgacgac 
      ttccacgtg aaaatcaca gcgaacgcg ccgaccttg 
      aaaatcgac aagtacgtc atcgaagca cagaataaa 
      cccgccaag accggacgg cgaatccaa gtgacggcg 
      aaatcaggc aacaagaat atcctggcc gggagcacg 
      agctacaca gcgcgggaa gagcacggg aaagtgatc 
      atcgaaggc tcgggttcg tttaaacta cacgacgaa 
      acgaaatcg gcgaacttt aaatacatc cgccagaat 
      ctggtccag gccaagaac ggcgaaacg ggcatcgag 
      atctccttc gacgccagt ttggggaac aaagcaatt 
      gatgcagaa ttgaaaatc accgacaaa cagttcagg 
      attttgaac tcctactgc gaggaaaag aaacaatgt 
      gcacatgtg gaagtcgat gttaagaca accgttgac 
      gatgtcaaa gccttcaac caggaaatc gaaatcgcg 
      attgactta cgcaagctg gggttgtcg cacgagttt 
      ggcctcaaa gcggtgacc actcgcaag gagtacgtc 
      ctagaccac actgtagat atgcatttc caaagccag 
      gagaacagc aagtaccaa tacagtctc tacgtccac 
      cccaaccag gctggagtt agcctaacg acccccaaa 
      cgaatcatc gcccttgaa agtaaaatc aacgtcccg 
      tccaagttc ctccaagac ggtggttcc gtctccggc 
      gaagtcttc ttctacacg gacaagaag aaccaacca 
      aacaaaaaa tccggactc atctcctac ctcaacctc 
      aaccccaaa ggaccaacc cttgacggt gagatcaaa 
      ttcacaaac ccggcccta aaacgccca cttctgatc 
      agcatcaag tcgaaatac gtgccaatg tcgctggaa 
      agcgtggtc acgtttgac gttttcgcc caaccggac 
      cagaagctc gtcatcaaa cacacggag aagtggtcc 
      gacccgaag gactacaag aagatggaa gccaaagaa 
      gtggtggaa attaagagt acaggcctc cacattgat 
      atccactac gaggagtac gctttcgcg tcggtcgaa 
      gaccaccac tacaaggcc gaagtcaag ttgaggtac 
      tccggacct gggtccaaa cacgaatcc ggcgcgctt 
      tacgaagtc acccccaag aatctcgaa gccgggatc 
      aaactcttc aaccaccaa ttgttccac ctgacgtcc 
      aaataccaa ctctcgcaa aaccaccaa gtgtttgat 
      agcgagttg ggttgctac ggccataga ccgttggtg 
      agtcacctg gaggtgaag aacctcaac actttgaag 
      tactcgatc gcgtgcaaa gacaagcca aacgacaag 
      ctcctggtt aattgtggc ttcaagccg ggggagatt 
      gccgatgtt agggccgaa atcatcaaa ggcgggcaa 
      acgcacgag ttggggcac gcgactgtt aaactagac 
      gatgctaat ttcctcaag tccgactac aaggtccaa 
      gccaagaac atcgaggag ttgctgttc aagccgatc 
      aaggacggg atccagcgt gaaggggac gagttggcg 
      aaactctcg tcggagatg atgcaacac ggccagaag 
      gagttgcag agctttggc gaagtcgcc aaacgggca 
      acgcccgac atcgccccg cttcgccag tactacacc 
      ggcgaactc gccaaaatc aaggacgag atcctggcc 
      gacaaaacc atcaaggac ttgagcgaa ttcctttac 
      aacatcttg tcagttttc acacaaact ttcggcgat 
      tcgttcaaa cactttagc gagtttatt gagaatatt 
      tacatcagt tttgataag actttcggg aacatcctc 
      caaattatc gagaaggat attttaccg cacgccaaa 
      gtcacgtgt gacaagttc ctgaaactg ttccttcac 
      gctgttgac attgccacc catgccgtt ttaacagtc 
      ggtgtccaa attgccgac atcgccgaa aaataccaa 
      cccgagttc cagcaactt gccaatgtt tttggacaa 
      gtcagtcag gacgtttta cgggcaagt ctcaacctg 
      tacgaggcc gtaaaaagt gtgattatc gaccaatgg 
      caacaaatt tacaacgaa attaaggca atgccagct 
      gttgaacaa ctcaaagcc gagtatgaa caatacgtt 
      aaacatgga ttaccggcc cgcgaaaac attgccaat 
      gctatcaaa gacttgctt tcgacgatt aaagattta 
      gcaccgact gaagaactt aaagctttc attcaagct 
      attagtgac tatgtttgt ccgaaactc aacaaccaa 
      aagattgac gataaagtg gcaattgat cgaattatt 
      caatcggga attgctggc ggtaaatca atcatggcc 
      attgtgacc ggaagtcgg ccggttcca gaattgcac 
      aacttccaa cttccactt cctgcaaca cttcttaac 
      cgacttccc aaacttgca gcgtttagg tttagtccg 
      ttctcgttc attttgggc gaggaattg tacgtttct 
      gagttcttc ctatcgctt gtgaataga ccgaggaac 
      tggatacca ccattccca cgtaagtta caataaata 
      agagaccga tttaacttt ttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY