Status
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Gene information
General information
TC032126
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030062,TC030061
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9412758..9415134
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032126
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02585

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032126 RA
      MLFLTALVF LLAFGNVTC DTKIIGGSE VKNRSEFAY 
      QALLLYKDA PYCAGTIID EKHVLTAAH CCNPEKNLI 
      VEPSDVTVV VGNLDLYDK SCVKKVQHI FIHQEFDLA 
      SITNDIAVI RLVDNLEFS EDIQAINLT TDYLSSGNC 
      NVSGWGTTY IGANSMNSK LLYANVPIV EWNSCQRIL 
      KLFQVELGK NEICAGGNT KDACQGDSG GPLVCSGQL 
      TGIVSYGIS CGIEFIPGV YTEVAKYTK WIQEQKTRS 
      SSPKIELTF CLFVIQIIM LLLTLLFCS VFSLSTQKD 
      VKIVGGTEV KDRSKFAYQ ALLLYNGSP YCGGTIIDK 
      KYILTAGHC CYSRDQQML PSALSVVVG NLNLTDREI 
      VKSVKAIYV HEEFDMDTL DNDLAIFEL TEPFENWTS 
      TVSPIKLNK EHVTSGNCI ASGWGTTFS GSNKMSSKL 
      LYVSVPIID WTACYEFYG AYNVEVNNK SICAGALAK 
      DSCQGDSGG PFVCESKLT GVVSYGIGC GFENLPGVY 
      VEVAKYSSW LENYTTPSS SSSFSLATS CLVIFVVTI 
      IL
    CDS sequence
    • >TC032126 RA
      atgcttttc ctgacggcg ctcgtattt ctacttgcc 
      tttggaaat gtaacttgc gacactaaa ataatcggc 
      ggatctgag gtgaaaaat cgatcagaa tttgcttat 
      caggctttg ctgctctac aaagacgca ccttactgt 
      gctggaaca ataatcgac gagaaacac gttttaaca 
      gctgctcat tgctgtaat cctgagaag aacctaata 
      gttgaacca tcagatgtg acagttgtt gttggaaat 
      ttggatttg tatgacaaa tcttgcgtc aaaaaagtc 
      caacacatt ttcattcat caagagttt gatttagcg 
      tcaataacg aacgacatt gctgtgata cgacttgta 
      gataatctt gaattttcg gaagatatc caagctatt 
      aacttaaca acagattac ctgagttcc ggtaattgt 
      aacgtatcg ggatgggga accacatat atcggggcg 
      aattctatg aactcgaag ttactttac gccaatgtg 
      cctatagtt gagtggaac tcatgccaa cgaatcctt 
      aaattattt caagtcgag ttaggaaaa aacgaaatt 
      tgtgcaggg ggaaataca aaggatgca tgccaaggc 
      gactcggga ggacctcta gtgtgttca ggtcaattg 
      actggcatt gtttcgtat ggcatatct tgcgggatt 
      gaattcatc cctggagtt tacacagaa gttgccaaa 
      tacaccaag tggatccag gaacaaaaa accagatcc 
      tcaagccca aaaattgaa ctaactttt tgtctgttt 
      gtgatccaa atcataatg ttgctccta acgcttcta 
      ttttgtagt gttttttcc ttgagcacg caaaaagat 
      gttaaaatt gttggaggg actgaagtc aaagacagg 
      tctaaattt gcttatcag gcacttctt ctgtataat 
      ggtagtcca tactgtggt gggaccata attgacaag 
      aaatacatt ttaacagcg gggcattgt tgttattcc 
      agggaccaa caaatgtta ccttctgcc ctttcggtg 
      gttgttggt aatttgaac ttaaccgat cgagaaatc 
      gtcaaaagt gtgaaagcg atttacgtt cacgaagaa 
      ttcgatatg gacactctc gataatgat ttggccata 
      tttgagctg accgagcct tttgaaaat tggacaagc 
      actgtatca ccaattaaa ttaaataaa gaacatgtg 
      acgagtggt aactgtatt gcatcaggc tggggaacc 
      acattttct ggttctaat aaaatgagc tccaagctg 
      ctatatgtg agtgttcct ataattgac tggacggcg 
      tgttatgaa ttttacggt gcttataac gtggaagtg 
      aataataag tcgatttgt gctggggct cttgctaag 
      gactcttgc cagggcgat tccgggggc ccttttgtc 
      tgcgaaagc aaactaacg ggcgtggtt tcgtatggc 
      atagggtgc ggttttgag aatttgccc ggagtttac 
      gtcgaagtg gcgaaatat tccagttgg ctggaaaat 
      tacacaaca ccgtcatct tcgtcttca ttcagttta 
      gcgacttct tgtcttgtg atatttgtt gtaacaata 
      attttgtaa
    mRNA sequence
    • >TC032126 RA
      tttagtaaa tcatcatgc ttttcctga cggcgctcg 
      tatttctac ttgcctttg gaaatgtaa cttgcgaca 
      ctaaaataa tcggcggat ctgaggtga aaaatcgat 
      cagaatttg cttatcagg ctttgctgc tctacaaag 
      acgcacctt actgtgctg gaacaataa tcgacgaga 
      aacacgttt taacagctg ctcattgct gtaatcctg 
      agaagaacc taatagttg aaccatcag atgtgacag 
      ttgttgttg gaaatttgg atttgtatg acaaatctt 
      gcgtcaaaa aagtccaac acattttca ttcatcaag 
      agtttgatt tagcgtcaa taacgaacg acattgctg 
      tgatacgac ttgtagata atcttgaat tttcggaag 
      atatccaag ctattaact taacaacag attacctga 
      gttccggta attgtaacg tatcgggat ggggaacca 
      catatatcg gggcgaatt ctatgaact cgaagttac 
      tttacgcca atgtgccta tagttgagt ggaactcat 
      gccaacgaa tccttaaat tatttcaag tcgagttag 
      gaaaaaacg aaatttgtg cagggggaa atacaaagg 
      atgcatgcc aaggcgact cgggaggac ctctagtgt 
      gttcaggtc aattgactg gcattgttt cgtatggca 
      tatcttgcg ggattgaat tcatccctg gagtttaca 
      cagaagttg ccaaataca ccaagtgga tccaggaac 
      aaaaaacca gatcctcaa gcccaaaaa ttgaactaa 
      ctttttgtc tgtttgtga tccaaatca taatgttgc 
      tcctaacgc ttctatttt gtagtgttt tttccttga 
      gcacgcaaa aagatgtta aaattgttg gagggactg 
      aagtcaaag acaggtcta aatttgctt atcaggcac 
      ttcttctgt ataatggta gtccatact gtggtggga 
      ccataattg acaagaaat acattttaa cagcggggc 
      attgttgtt attccaggg accaacaaa tgttacctt 
      ctgcccttt cggtggttg ttggtaatt tgaacttaa 
      ccgatcgag aaatcgtca aaagtgtga aagcgattt 
      acgttcacg aagaattcg atatggaca ctctcgata 
      atgatttgg ccatatttg agctgaccg agccttttg 
      aaaattgga caagcactg tatcaccaa ttaaattaa 
      ataaagaac atgtgacga gtggtaact gtattgcat 
      caggctggg gaaccacat tttctggtt ctaataaaa 
      tgagctcca agctgctat atgtgagtg ttcctataa 
      ttgactgga cggcgtgtt atgaatttt acggtgctt 
      ataacgtgg aagtgaata ataagtcga tttgtgctg 
      gggctcttg ctaaggact cttgccagg gcgattccg 
      ggggccctt ttgtctgcg aaagcaaac taacgggcg 
      tggtttcgt atggcatag ggtgcggtt ttgagaatt 
      tgcccggag tttacgtcg aagtggcga aatattcca 
      gttggctgg aaaattaca caacaccgt catcttcgt 
      cttcattca gtttagcga cttcttgtc ttgtgatat 
      ttgttgtaa caataattt tgtaaatac agttttttt 
      atgttttac tttttacta gcaattcgt gtaaaaatg 
      atatcttca cttagtaat ttgcaaata aagcattat 
      ttgtgct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY