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Gene information
General information
TC030051 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 4311888..4320045
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030051
    iBeetle screen iB_02584
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    Adult morphology
    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 4 (41 dpi)
    Days post injection



    Fertility
    fertility after larval injection decreased (no living offspring) -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030051 RA
      MEIVVNSPK VKYCDNYIE THYDYLYTK AEKRGNKIE 
      VAPVTEHLV IRTESKVPR VGVMLVGWG GNNGSTFTA 
      AVLANKEGL SWQTKKGVQ KANWYGSVT QASTVRLGG 
      DVYVPLGKL LPTVHPDDL VIDGWDISS ANLAEAMER 
      AQVLEPGLQ QQLKAKMAA LKPRPSIYD QDFIAANQA 
      SRADNIIKG TKLEQVQKI CQDIENFKK KTNVDKVII 
      LWTANTERF TEIVDGIND TYANLKLAI SSNHPEISP 
      STLFAYAAI SMKCTYING SPQNTFVPG IIEMAEKNN 
      VFIAGDDFK SGQTKIKSV LVDFLVSAG IKPTSIVSY 
      NHLGNNDGK NLSAPKQFR SKEISKSNV VDDMVDSND 
      ILYKPGEKP DHVVVIKYV PYVGDSKRA MDEYTSEIM 
      MGGLNTIVV HNTCEDSLL ATPIILDLI ILAELCSRI 
      HLKKQGQGD REFVGFHSV LSLLSYLCK APLVPHNTP 
      VVNALFRQR ACIENVMKA CLGLPPDSN MLLEHKVPF 
      LMKDEVSNE PHKKKIKIQ NGGH
    CDS sequence
    • >TC030051 RA
      atggaaatt gttgtcaac agccccaaa gtgaaatac 
      tgtgacaat tacatcgaa acgcattat gactattta 
      tacaccaaa gcggaaaaa agggggaac aaaattgag 
      gtcgccccc gtcaccgaa catctggtg atacggacc 
      gagagcaaa gtcccccgt gtgggggtg atgctagtg 
      ggctggggc gggaacaac gggagtact tttaccgcg 
      gccgttctg gccaacaag gagggtttg tcttggcag 
      acgaagaag ggagtccag aaggcgaat tggtatggg 
      tctgttacc caggctagc acggttaga ttgggtggc 
      gatgtctac gtaccattg ggcaagttg ttgccaacg 
      gtacatccc gacgatttg gtgattgat gggtgggat 
      atcagttcg gccaatttg gccgaagcg atggaaagg 
      gcgcaggtt ttggagcca gggctccag caacagctc 
      aaggcgaaa atggcagct ttgaagccg cgaccgtcg 
      atctacgac caggatttc attgcagca aatcaggca 
      tctcgcgcc gacaacatc atcaaagga accaaactc 
      gaacaagtg cagaaaatc tgccaagac atcgagaat 
      ttcaaaaag aaaacaaac gttgacaaa gtcataatc 
      ctatggaca gcaaacact gaacgtttc actgaaatc 
      gtagacgga ataaacgat acttatgct aatctcaaa 
      ttggcaatt tcctcaaac catccagaa atctctcca 
      tcaacacta tttgcttac gctgccatt tccatgaaa 
      tgtacttac atcaacggc tccccacaa aacaccttc 
      gtaccggga atcatcgaa atggcagaa aaaaataac 
      gtttttatc gctggagat gatttcaaa tcaggacag 
      accaaaatt aagagcgtt ttggtcgac tttttggtc 
      agtgcaggg attaaaccg accagtatt gtaagctat 
      aatcattta gggaataat gatgggaag aatttgtcc 
      gcgcccaaa caattccgc tccaaggag atatcgaaa 
      agtaacgtt gttgacgat atggtcgat tctaacgac 
      attctgtac aaaccgggg gaaaaaccc gatcatgtg 
      gttgtcatt aagtacgtc ccatatgtc ggcgactct 
      aagcgtgcg atggacgag tacacctct gaaataatg 
      atgggaggt ctcaacacc attgtggtg cacaacact 
      tgcgaggat tcgcttttg gccacccct attatccta 
      gatctgata attttggcc gagttgtgc tcgaggatt 
      catctcaag aaacaagga caaggcgac cgagagttt 
      gtgggtttc cacagtgtt ctctcgctt ttgagttac 
      ttatgcaag gcacctttg gtgcctcac aacactcct 
      gtcgtcaat gctcttttc agacagagg gcatgtatt 
      gagaacgtg atgaaagcg tgtctgggt ttgccacct 
      gattccaat atgttgctt gagcacaaa gtgccgttt 
      ttaatgaaa gatgaagta agtaacgag ccacataaa 
      aagaagatt aaaattcag aatggcggg cattaa
    mRNA sequence
    • >TC030051 RA
      gttcaagtt cagtagtcg ttcaaacaa cgttgtttc 
      cgtttgtgg aagttgtgc gctacaaat ttgcgtctt 
      ttctgttcc agttgaaat tttcgttgg ctaagcgca 
      aataaaaag tcaaaaaaa tggaaattg ttgtcaaca 
      gccccaaag tgaaatact gtgacaatt acatcgaaa 
      cgcattatg actatttat acaccaaag cggaaaaaa 
      gggggaaca aaattgagg tcgcccccg tcaccgaac 
      atctggtga tacggaccg agagcaaag tcccccgtg 
      tgggggtga tgctagtgg gctggggcg ggaacaacg 
      ggagtactt ttaccgcgg ccgttctgg ccaacaagg 
      agggtttgt cttggcaga cgaagaagg gagtccaga 
      aggcgaatt ggtatgggt ctgttaccc aggctagca 
      cggttagat tgggtggcg atgtctacg taccattgg 
      gcaagttgt tgccaacgg tacatcccg acgatttgg 
      tgattgatg ggtgggata tcagttcgg ccaatttgg 
      ccgaagcga tggaaaggg cgcaggttt tggagccag 
      ggctccagc aacagctca aggcgaaaa tggcagctt 
      tgaagccgc gaccgtcga tctacgacc aggatttca 
      ttgcagcaa atcaggcat ctcgcgccg acaacatca 
      tcaaaggaa ccaaactcg aacaagtgc agaaaatct 
      gccaagaca tcgagaatt tcaaaaaga aaacaaacg 
      ttgacaaag tcataatcc tatggacag caaacactg 
      aacgtttca ctgaaatcg tagacggaa taaacgata 
      cttatgcta atctcaaat tggcaattt cctcaaacc 
      atccagaaa tctctccat caacactat ttgcttacg 
      ctgccattt ccatgaaat gtacttaca tcaacggct 
      ccccacaaa acaccttcg taccgggaa tcatcgaaa 
      tggcagaaa aaaataacg tttttatcg ctggagatg 
      atttcaaat caggacaga ccaaaatta agagcgttt 
      tggtcgact ttttggtca gtgcaggga ttaaaccga 
      ccagtattg taagctata atcatttag ggaataatg 
      atgggaaga atttgtccg cgcccaaac aattccgct 
      ccaaggaga tatcgaaaa gtaacgttg ttgacgata 
      tggtcgatt ctaacgaca ttctgtaca aaccggggg 
      aaaaacccg atcatgtgg ttgtcatta agtacgtcc 
      catatgtcg gcgactcta agcgtgcga tggacgagt 
      acacctctg aaataatga tgggaggtc tcaacacca 
      ttgtggtgc acaacactt gcgaggatt cgcttttgg 
      ccaccccta ttatcctag atctgataa ttttggccg 
      agttgtgct cgaggattc atctcaaga aacaaggac 
      aaggcgacc gagagtttg tgggtttcc acagtgttc 
      tctcgcttt tgagttact tatgcaagg cacctttgg 
      tgcctcaca acactcctg tcgtcaatg ctcttttca 
      gacagaggg catgtattg agaacgtga tgaaagcgt 
      gtctgggtt tgccacctg attccaata tgttgcttg 
      agcacaaag tgccgtttt taatgaaag atgaagtaa 
      gtaacgagc cacataaaa agaagatta aaattcaga 
      atggcgggc attaatttt tttgtttta tgttgttgt 
      ttccttcag tttaatttg aacatatcc tgtcctggt 
      tgcttaaaa tgttgatgt ttattggat gttttcctt 
      gatatgatg gttcaaatc attctgatg ggctgtaca 
      cggacggaa cataatttt gttaattgt gataatgaa 
      agtatttta cttttgtac ctttcacag taataaaag 
      tttattgat tga