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Gene information
General information
TC016377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 6946280..6947525
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC016377
      iBeetle screen iB_02582

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection

      beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

      procephalic head mostly not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      head irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      labrum position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      labrum split - Penetrance (percentage of animals/eggs on slide): less than 30%

      labrum irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Comment: about 50-60%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016377 RA
        MGTQKVVIP RGLINPSET VVVEIPEQF KDVSTVVAR 
PKSADMSIP LPPTPKNRL VAKPGRTWP KADNRGGFG 
ALYDDEGIT WGGRPNEEE SSYATPSPR PPPPPKRFS 
KTPPGTYPQ RIPRRPWYM GDERAGITW GGKGNRPMK 
QGQRPRPPG WAYQELSPG PVTYPGKFG SVRYHTDFD 
PNPALHSTL RDLDDPIER TMYCDFRPS RGMSPRPNS 
LGESPDSLR HWHSSLGSV SPPLRQKGQ YYSDSSPEE 
SPEGSPEQY GARQALSFG DSSEEGGAY CGSPECAAS 
PESSGGDSG EEAYYQSPP PPPPPTVEK VQEVVDDLE 
TRELKKFIA RIRKYQNAR RGLVFTAAT LDTAQDIAR 
SAKRY
      CDS sequence
      • >TC016377 RA
        atggggacc caaaaagtg gtaattcca aggggtttg 
atcaacccc agcgaaacg gtagttgtg gaaattcct 
gaacagttc aaagatgtc agcacagtg gtagctcgc 
ccaaaatct gcagacatg tcgatccct ctgccccca 
acccccaaa aacaggcta gttgcaaaa ccaggtcgc 
acttggcct aaagcagac aatcgaggt ggtttcggt 
gcattatac gacgacgaa ggtatcacc tggggcggc 
cgacctaac gaagaagaa tcaagttac gccactcca 
tcaccacgt ccccctccg cccccgaaa cggttctct 
aaaacaccg cccggcaca tatccccaa agaatcccc 
aggaggcct tggtacatg ggtgatgaa agagctggc 
atcacttgg ggcggcaaa ggaaacagg cccatgaaa 
caagggcag cggcctcgt cccccgggc tgggcctat 
caagagttg tcaccagga ccagtaaca tatccagga 
aagttcgga agtgtcagg taccacacg gattttgat 
cctaatcca gctctgcat tccactctg agagacctt 
gatgaccca atagagagg accatgtac tgtgatttc 
cgaccttca cgaggcatg tctccgcgt ccaaattct 
ttgggagaa agtccagat tcgttaaga cattggcac 
tccagtctg ggctcagtc agtcctccc cttcgacaa 
aaaggacaa tattactcc gactcctcg cctgaggag 
tcacctgag gggtcacca gagcaatac ggggctcga 
caagcactt agttttggt gattcttct gaggaagga 
ggtgcttac tgcgggtcg cccgagtgc gcagcttca 
ccagaatca tctggtgga gattctggt gaagaagcg 
tattatcag tctccacca cctcctccg ccaccaaca 
gttgaaaaa gttcaagag gtggtagac gacttggaa 
accagagaa ctcaaaaag tttattgca aggatcaga 
aaatatcaa aatgcgaga agaggactt gtctttact 
gcagctact ttagatact gcccaagac atagcccga 
tctgccaaa agatattag
      mRNA sequence
      • >TC016377 RA
        tttgttttg ttgacgtgt aaacatctt cgtcaaact 
ttcgcaact ttatcaaaa tttttcgtg taacaaatc 
aagatgggg acccaaaaa gtggtaatt ccaaggggt 
ttgatcaac cccagcgaa acggtagtt gtggaaatt 
cctgaacag ttcaaagat gtcagcaca gtggtagct 
cgcccaaaa tctgcagac atgtcgatc cctctgccc 
ccaaccccc aaaaacagg ctagttgca aaaccaggt 
cgcacttgg cctaaagca gacaatcga ggtggtttc 
ggtgcatta tacgacgac gaaggtatc acctggggc 
ggccgacct aacgaagaa gaatcaagt tacgccact 
ccatcacca cgtccccct ccgcccccg aaacggttc 
tctaaaaca ccgcccggc acatatccc caaagaatc 
cccaggagg ccttggtac atgggtgat gaaagagct 
ggcatcact tggggcggc aaaggaaac aggcccatg 
aaacaaggg cagcggcct cgtcccccg ggctgggcc 
tatcaagag ttgtcacca ggaccagta acatatcca 
ggaaagttc ggaagtgtc aggtaccac acggatttt 
gatcctaat ccagctctg cattccact ctgagagac 
cttgatgac ccaatagag aggaccatg tactgtgat 
ttccgacct tcacgaggc atgtctccg cgtccaaat 
tctttggga gaaagtcca gattcgtta agacattgg 
cactccagt ctgggctca gtcagtcct ccccttcga 
caaaaagga caatattac tccgactcc tcgcctgag 
gagtcacct gaggggtca ccagagcaa tacggggct 
cgacaagca cttagtttt ggtgattct tctgaggaa 
ggaggtgct tactgcggg tcgcccgag tgcgcagct 
tcaccagaa tcatctggt ggagattct ggtgaagaa 
gcgtattat cagtctcca ccacctcct ccgccacca 
acagttgaa aaagttcaa gaggtggta gacgacttg 
gaaaccaga gaactcaaa aagtttatt gcaaggatc 
agaaaatat caaaatgcg agaagagga cttgtcttt 
actgcagct actttagat actgcccaa gacatagcc 
cgatctgcc aaaagatat tagcacatt aatgccgtt 
tccagaaca tctgttgta atagtattt ctaaaatta 
agattaaac cctttggaa caaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY