Status
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Gene information
General information
TC016367 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 7104778..7111485
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016367
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02581

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016367 RA
      MSASSDPPQ KCVTEVPNY GLKVRLNHT YPENRSQDF 
      KPGLRLIWD MLPLILRYI WYYIVHRIM GKSIVMDYV 
      HTLRAKQIY GVPIGGIGC GTIGRGYRG EFCRFQLKP 
      GLYEYNTVD ANQFIVTIK DGHNETIFH SLLSTFPTK 
      SLKSWESLI DGSECFYTG LYPRSWTEY DLSKYGVML 
      TCRQITPII PHNYKDSSL PCAMFVWDV KNISEEDRT 
      VTIAFTFKN GVGDKSRDR SSTCSSKAF TLADSEGVI 
      LYHTIDKMQ CSYSLAAKT NPQIDISKC LYFDPNSDG 
      IKPWIQLKN NGSFDKISD KNYGQIFGE MACGIATKA 
      TIRAGNTVT SEMALVWDM PSVQFPKKQ KKYTRYYTK 
      HFDGDNTGL RIVRYAFEN YQNWERAIY DWQRRILDD 
      ENLPDWYKS ALFNESYYI SDGGAIWVI LDEQEAKKL 
      PKNDPRLEH GKFALLEGH EYKMYNTYD VHFYASFSF 
      VLNFPLLQS ILQYDMRDA IFTEIPDKV KMLYDGEVC 
      ERKVPNTVP HDIGDPGEE PFILLNSYP IHDVSQWRD 
      LNSKFVLQV FRDAFITGL DDRSIAYLN DMYNACYTV 
      MHKSLDFDV DGDGLIENS GSPDQTFDT WVMTGASAY 
      CGGLWLAAL FAMTKIADA LQKTEDKEK FQELLDKGT 
      AAFERKLWN GKCYNFDCS DKECRSIMA DQLCGQWYL 
      RSCGFNYEV FPQDRVKTS LKTIYENNV QSFCDGRMG 
      AVNGFIDGV IDEFTIQSQ EVWTGVTYA LAASMLQEG 
      MRTEAFNTA GGMFKSMSE RFGLSFDTP EALYAAKYY 
      RAIGYMRPL SIWSMQLAI DQLNKNVN
    CDS sequence
    • >TC016367 RA
      atgagtgct agcagtgat cctccccaa aaatgtgtc 
      acagaagtc cccaattac ggcctaaaa gtgcgccta 
      aaccacact tatccagaa aaccggtca caagatttt 
      aaacccggt cttaggcta atctgggac atgctacct 
      ctaattttg aggtacatt tggtactat attgttcat 
      agaattatg ggaaagtcg atcgttatg gactacgtg 
      cacactttg agagcgaaa caaatttac ggtgtcccc 
      atcggtggg atcggttgc ggaacgatt ggaaggggg 
      tacaggggc gaattctgc cggtttcaa ctcaaacca 
      gggctttat gagtacaat acagtcgat gccaatcaa 
      tttattgtt acaatcaaa gatgggcac aatgagacc 
      atttttcat agtctttta tcaacgttt ccgacaaag 
      agtttaaaa agctgggaa agcctaatt gacggttca 
      gagtgtttc tatactgga ctttaccca agatcgtgg 
      actgagtac gacttatca aaatacggc gtcatgtta 
      acttgtcgg caaattacc ccaatcatt ccgcataat 
      tacaaggat agttccctg ccttgtgca atgttcgtc 
      tgggacgtc aaaaacatc tcagaggag gaccgaacc 
      gtcacaatc gccttcact ttcaaaaac ggcgttggt 
      gataaatcc cgcgacaga tcttcaaca tgtagttca 
      aaagccttc actctcgcc gactccgaa ggcgtcatt 
      ctttaccac acaattgat aaaatgcaa tgttcctac 
      tctcttgcc gccaaaacc aacccgcaa atcgacatt 
      tcaaaatgt ctctatttc gacccgaat tcagacggg 
      atcaagccc tggatccaa ctcaaaaac aacggcagt 
      ttcgataaa atcagcgat aaaaactac ggccaaatt 
      ttcggcgaa atggcctgt ggtatcgcc acgaaggca 
      accatccgg gccggaaat acggtaacg tcagaaatg 
      gccctggtg tgggacatg ccctcggtc caattcccc 
      aaaaaacag aagaaatac accagatac tacaccaaa 
      catttcgac ggcgataac accgggctg aggatcgtg 
      cgatacgcg tttgagaat taccaaaac tgggaacgg 
      gccatttac gactggcag agacggatc ctcgacgat 
      gaaaatttg cccgattgg tacaagtca gcccttttt 
      aatgagagt tactacata agtgacggt ggggcaatt 
      tgggtcatt ttagacgaa caagaagcc aaaaaattg 
      ccgaaaaac gaccctaga ctcgagcac ggtaaattt 
      gcattgttg gagggccac gagtacaaa atgtacaac 
      acttacgac gttcatttt tatgcttca ttttcgttt 
      gttttaaat tttcctttg ctccaatca atcttacag 
      tacgatatg agagacgcc attttcacc gaaattccg 
      gataaagtt aaaatgttg tatgacggg gaagtgtgc 
      gagaggaaa gtaccaaat acagtaccg cacgatatt 
      ggagatcca ggggaagaa ccgtttatc ttactcaat 
      tcgtacccc atccatgac gtaagccag tggcgcgat 
      ttgaattca aagttcgtt ttacaagtg ttcagagac 
      gcttttata acaggtctt gacgaccga tctatcgcg 
      tatttgaac gatatgtac aacgcgtgt tacacagta 
      atgcacaaa agtctggat tttgacgtg gatggagac 
      ggccttatc gaaaattcg ggaagtccc gaccaaacc 
      ttcgatact tgggtcatg acaggtgct agtgcgtat 
      tgtgggggg ctgtggttg gccgctctg ttcgctatg 
      actaaaatc gcggacgct ttgcaaaaa accgaagac 
      aaggaaaag tttcaggaa ttgttggat aagggcaca 
      gcggcgttc gaacggaaa ttgtggaac ggaaagtgc 
      tacaatttc gactgttcg gacaaagag tgtcgctcg 
      attatggcc gatcagtta tgcggccag tggtatctc 
      aggagttgc ggatttaat tatgaggtg tttccgcaa 
      gatcgggtc aagactagc ttgaaaacg atttacgaa 
      aataacgtt cagtcgttt tgtgacggc cgaatgggg 
      gctgttaat ggatttatt gacggggtt attgatgag 
      ttcactatt cagagtcag gaggtgtgg actggggtt 
      acctacgcc ctagctgcc agcatgctt caagaagga 
      atgaggact gaggctttt aatactgct ggcggaatg 
      ttcaagtca atgagtgag cgatttggg ctttccttc 
      gatactccg gaagcctta tatgcggca aagtattac 
      agagcgatc ggttacatg cgaccccta agtatatgg 
      tcaatgcag ttggctatt gaccaactt aataaaaat 
      gtaaattaa
    mRNA sequence
    • >TC016367 RA
      atctttttt ttaacatga agatagtat cgattgtaa 
      agtgaaaca ttcaaaata tttaaataa taaaagtat 
      cttgctgat ataaggttt tatttaaat tttttgtca 
      gtaaaattg taataattt gactgtgag attgcaaca 
      ataattgtt aagatcgga ttggcagca ctgtgccca 
      cttgtcaca agtgacagc gtgtcaatt gtttaataa 
      atatttttt tatcaaaaa caaccccaa aatgccaca 
      ggtgacttc agttatcgc caaattctt caatgaata 
      taattcaaa aaccccgaa gaagctgaa atgtattga 
      cgcttattt gttgtatat tttgttaac cggtatcgt 
      tcaatttgt ttattgctg tcttgtggg cacttttcc 
      gtttaattc ctttcttag tggatttat ctcttgtgt 
      cagttgttt tgtcttggc agtgtgttt aaggctaca 
      agtgaaccc agaaaacaa gaatcagtt cgccggaat 
      aagcccgga aaggggctt tgctgactt tattttcgc 
      ccatgtggt cctacattt agttgtaat gaacttcat 
      tggttaatt atgtatttt gtaaaatga gataatttc 
      aataaatta caaaaaaaa tactggttg agtggtttc 
      tccttccca tcggttgta aaccttgac gtgcgataa 
      ccaccaaat ttgcaaaaa cgcaagttt acaagactg 
      tcgtaatct ctcaaaatt tttaagtac actcccatt 
      ttgggtgat ttattcagc gcatttttg tgataaatc 
      gctgataac gctcgaaat agtcacatc gttcaaaat 
      tcacacttc tgatcagtc acccattaa aaatgagtg 
      ctagcagtg atcctcccc aaaaatgtg tcacagaag 
      tccccaatt acggcctaa aagtgcgcc taaaccaca 
      cttatccag aaaaccggt cacaagatt ttaaacccg 
      gtcttaggc taatctggg acatgctac ctctaattt 
      tgaggtaca tttggtact atattgttc atagaatta 
      tgggaaagt cgatcgtta tggactacg tgcacactt 
      tgagagcga aacaaattt acggtgtcc ccatcggtg 
      ggatcggtt gcggaacga ttggaaggg ggtacaggg 
      gcgaattct gccggtttc aactcaaac cagggcttt 
      atgagtaca atacagtcg atgccaatc aatttattg 
      ttacaatca aagatgggc acaatgaga ccatttttc 
      atagtcttt tatcaacgt ttccgacaa agagtttaa 
      aaagctggg aaagcctaa ttgacggtt cagagtgtt 
      tctatactg gactttacc caagatcgt ggactgagt 
      acgacttat caaaatacg gcgtcatgt taacttgtc 
      ggcaaatta ccccaatca ttccgcata attacaagg 
      atagttccc tgccttgtg caatgttcg tctgggacg 
      tcaaaaaca tctcagagg aggaccgaa ccgtcacaa 
      tcgccttca ctttcaaaa acggcgttg gtgataaat 
      cccgcgaca gatcttcaa catgtagtt caaaagcct 
      tcactctcg ccgactccg aaggcgtca ttctttacc 
      acacaattg ataaaatgc aatgttcct actctcttg 
      ccgccaaaa ccaacccgc aaatcgaca tttcaaaat 
      gtctctatt tcgacccga attcagacg ggatcaagc 
      cctggatcc aactcaaaa acaacggca gtttcgata 
      aaatcagcg ataaaaact acggccaaa ttttcggcg 
      aaatggcct gtggtatcg ccacgaagg caaccatcc 
      gggccggaa atacggtaa cgtcagaaa tggccctgg 
      tgtgggaca tgccctcgg tccaattcc ccaaaaaac 
      agaagaaat acaccagat actacacca aacatttcg 
      acggcgata acaccgggc tgaggatcg tgcgatacg 
      cgtttgaga attaccaaa actgggaac gggccattt 
      acgactggc agagacgga tcctcgacg atgaaaatt 
      tgcccgatt ggtacaagt cagcccttt ttaatgaga 
      gttactaca taagtgacg gtggggcaa tttgggtca 
      ttttagacg aacaagaag ccaaaaaat tgccgaaaa 
      acgacccta gactcgagc acggtaaat ttgcattgt 
      tggagggcc acgagtaca aaatgtaca acacttacg 
      acgttcatt tttatgctt cattttcgt ttgttttaa 
      attttcctt tgctccaat caatcttac agtacgata 
      tgagagacg ccattttca ccgaaattc cggataaag 
      ttaaaatgt tgtatgacg gggaagtgt gcgagagga 
      aagtaccaa atacagtac cgcacgata ttggagatc 
      caggggaag aaccgttta tcttactca attcgtacc 
      ccatccatg acgtaagcc agtggcgcg atttgaatt 
      caaagttcg ttttacaag tgttcagag acgctttta 
      taacaggtc ttgacgacc gatctatcg cgtatttga 
      acgatatgt acaacgcgt gttacacag taatgcaca 
      aaagtctgg attttgacg tggatggag acggcctta 
      tcgaaaatt cgggaagtc ccgaccaaa ccttcgata 
      cttgggtca tgacaggtg ctagtgcgt attgtgggg 
      ggctgtggt tggccgctc tgttcgcta tgactaaaa 
      tcgcggacg ctttgcaaa aaaccgaag acaaggaaa 
      agtttcagg aattgttgg ataagggca cagcggcgt 
      tcgaacgga aattgtgga acggaaagt gctacaatt 
      tcgactgtt cggacaaag agtgtcgct cgattatgg 
      ccgatcagt tatgcggcc agtggtatc tcaggagtt 
      gcggattta attatgagg tgtttccgc aagatcggg 
      tcaagacta gcttgaaaa cgatttacg aaaataacg 
      ttcagtcgt tttgtgacg gccgaatgg gggctgtta 
      atggattta ttgacgggg ttattgatg agttcacta 
      ttcagagtc aggaggtgt ggactgggg ttacctacg 
      ccctagctg ccagcatgc ttcaagaag gaatgagga 
      ctgaggctt ttaatactg ctggcggaa tgttcaagt 
      caatgagtg agcgatttg ggctttcct tcgatactc 
      cggaagcct tatatgcgg caaagtatt acagagcga 
      tcggttaca tgcgacccc taagtatat ggtcaatgc 
      agttggcta ttgaccaac ttaataaaa atgtaaatt 
      aattcaaac cacatttat tagttaatt ttgtatttg 
      taattctta aatgcgtta ataaagtgt caccaaaga 
      a
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