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Gene information
General information
TC016344 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid49: 155094..157130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016344
    iBeetle screen iB_02577
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016344 RA
      MRFVLLGTV LCLYVHHIS GHGMLMEPP 
      NRSSLWRFN PNAPVNYDD DQNYCGGMN 
      VQWDQFGGK CGVCGDQYD DPHPQANEN 
      TGKYGQGII AKEYTAGSV IDVQVVLTT 
      NHMGYFNFS LCVLEDPNA PESGEECFK 
      PITLGDGSD KYVLPNTEE EDNDTINTT 
      VKLPDGLTC DRCVLRWTY TAGNNWGQC 
      EDGSYDEGC GPQETFRSC TDIAIH
    CDS sequence
    • >TC016344 RA
      atgagattc gttttgttg ggtactgtc ttgtgtctc 
      tacgtccat cacatttcc ggtcatggg atgttgatg 
      gaaccccca aatcgcagc tctttgtgg cgcttcaac 
      cccaacgct cctgtcaac tacgacgat gaccaaaat 
      tactgtggg ggtatgaat gtccaatgg gaccaattc 
      ggtggcaaa tgcggtgtc tgtggtgac caatacgac 
      gacccccac ccacaagcc aacgaaaat acgggaaaa 
      tatggtcaa ggaataatc gccaaggaa tatacagct 
      gggagtgtt atcgatgtc caggttgtg cttaccacc 
      aaccatatg ggctatttc aacttcagt ttgtgcgtt 
      ttggaagat cctaatgcg ccagaatca ggagaagag 
      tgtttcaaa ccgatcacg ttgggtgat gggtcagat 
      aagtatgtt ttgcccaac acggaagaa gaggacaat 
      gataccatt aataccact gttaaattg cccgatggt 
      ctcacttgt gaccggtgt gttttgaga tggacctac 
      actgccgga aataactgg ggacagtgc gaggacggg 
      tcttacgat gaaggctgt ggaccacaa gaaactttc 
      cgctcttgt acagatatt gccatccac taa
    mRNA sequence
    • >TC016344 RA
      acttcgtca aaattctcc gtgtcaatg actcaccac 
      gaagctcat caatgtccc tccagctgc ccccacggt 
      aaaggtgcc atcgtaata acaataacc ggatgcaaa 
      gggagctgt cataagaaa cacgattct gctgtggaa 
      gagaaacat catcaaccc gaactggtt gaaagcaaa 
      ttggctgat agcgaatcg gttgtgacc actgatacc 
      cttggtgga acctcagac gagccgaaa ttcgtttta 
      ccgttattg gtggggcgc caccatggc cctggatgt 
      gccatggag ataaatgtt ctggaaaaa cacgatgac 
      tcttgttta tataaaaag caaccagtc cctggtcgc 
      tcactggag gatcgggtc gggagttac gagtgcatt 
      gggaggatt gttttcaga acctgtgcc acagaggaa 
      gcgtccgaa aaaaaaccg gatctctcg cgtggttct 
      ggagacaaa cctccaatt cgcactcct tcgcctcct 
      ataacagcg gccacgcaa actacaccc tcaccctcc 
      tcctctggc cagggaagt aaatacgcc agtgcaacc 
      tccgtacct gagaaagcg ccagcatcc cccaacccg 
      cagaaacag caggtggag agaagcaca agtcctcga 
      atggaacgg gttttgcat gtgcaacct acggtcgca 
      aatccgaaa atcatcaac tgaagagac cagacatcc 
      cattccgcg aaaccccgt ctggatggt ccccggaca 
      ttttgacaa caaattata cacctcgaa aaaaatctt 
      cccggttta cgccgatgg aggtatcca gacggacaa 
      acagttgaa aggttcgga ggagactat cacgacttt 
      gaccgagct tcctaaagc tcctgccaa aactaagaa 
      atctggatt gttcgggtc tgtgtttcg gggaaagaa 
      gaatgcggt taaaacaga tcagaaaaa ggttaaaaa 
      agaaaaaca aggagcgta ataaaacta tctatctct 
      acacctgct atcaaattc ttttagctt gtaacatga 
      taaaattta tttttacaa aatacttac gcaccactt 
      taaaaacaa ttaactttt ggtttcaaa acacttacc 
      gaccaacac tcatcttat caaaattta tataaaaga 
      gaaaatgtt tcattcaat gcaactcgc aaaaatgag 
      attcgtttt gttgggtac tgtcttgtg tctctacgt 
      ccatcacat ttccggtca tgggatgtt gatggaacc 
      cccaaatcg cagctcttt gtggcgctt caaccccaa 
      cgctcctgt caactacga cgatgacca aaattactg 
      tgggggtat gaatgtcca atgggacca attcggtgg 
      caaatgcgg tgtctgtgg tgaccaata cgacgaccc 
      ccacccaca agccaacga aaatacggg aaaatatgg 
      tcaaggaat aatcgccaa ggaatatac agctgggag 
      tgttatcga tgtccaggt tgtgcttac caccaacca 
      tatgggcta tttcaactt cagtttgtg cgttttgga 
      agatcctaa tgcgccaga atcaggaga agagtgttt 
      caaaccgat cacgttggg tgatgggtc agataagta 
      tgttttgcc caacacgga agaagagga caatgatac 
      cattaatac cactgttaa attgcccga tggtctcac 
      ttgtgaccg gtgtgtttt gagatggac ctacactgc 
      cggaaataa ctggggaca gtgcgagga cgggtctta 
      cgatgaagg ctgtggacc acaagaaac tttccgctc 
      ttgtacaga tattgccat ccactaata atattgact 
      gtacataat aattataat aaatccttt caaagtta