Status
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Gene information
General information
TC016333 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450240.1: 1255..2975
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016333
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02576

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    Adult morphology
    antenna distal sometimescolour lighter - Number of animals showing the phenotype: 7

    antenna distal sometimesnot present - Number of animals showing the phenotype: 7


    elytra dorsal not closed - Number of animals showing the phenotype: 2


    legs tarsus distal sometimesnot present - Number of animals showing the phenotype: 7


    legs distal sometimescolour lighter - Number of animals showing the phenotype: 7

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016333 RA
      MAKEALNIR PFIPEYFNR TTDLNIFFV DWSSLAAGP 
      CYPAAVWNT RHVGECTSQ LVERIKELG AKNIHLIGF 
      SLGGQLTNF VANALRPYK VSRITGLDP AGPGFLTAG 
      PENKLDKGD AEFVDVIHT NAFVQGIVE ESGHVDFYI 
      NGGVIQPGC WAENRFFAC NHHRAPLYF AESITTQMG 
      FWGWPCPSY TEYLIGRCP PKEPQIIMG EFVNRTSSG 
      VHLVITDSV SPFAVGKFT GPAIEIFVK SELIRRDIL 
      EKYKKDVEN FIDEDDVVE SLYEHSRLK NEHSKKKSE 
      NIIDSVSAE VDLI
    CDS sequence
    • >TC016333 RA
      atggcaaag gaagcacta aatattcgc ccattcatt 
      ccagaatat ttcaaccgc acaaccgac ttgaacata 
      ttcttcgtg gactggtcc tcccttgcc gcaggtcct 
      tgttacccg gctgccgtg tggaacacg cgccatgtg 
      ggcgaatgc acttcccaa ttagtcgaa cgaataaag 
      gagctagga gccaaaaac atccacctg atcggtttt 
      agtttaggc ggccagtta acaaacttc gtcgctaac 
      gcccttcgc ccgtacaaa gtttccagg attacagga 
      ctcgatcct gccggtccc ggtttccta acagcgggc 
      cccgaaaac aaattagac aaaggcgac gccgaattc 
      gtcgacgtc atccacaca aacgctttc gtgcaaggc 
      attgtggag gagagcggc catgtcgac ttttacata 
      aatgggggc gtgatccag cccggatgt tgggcagag 
      aacagattt ttcgcttgt aatcaccac cgggcgccc 
      ttgtacttt gccgaatca atcacaaca caaatgggt 
      ttttggggc tggccgtgc ccgtcatac accgagtac 
      ttaataggg aggtgtccg cccaaagag ccccagatc 
      ataatgggc gagttcgtc aacaggacg tccagtgga 
      gtccacttg gttataacc gattcggtt tcaccgttt 
      gctgtgggg aaattcaca ggaccagcg attgaaata 
      ttcgtcaag tcggaacta ataagacgg gatattttg 
      gaaaagtat aaaaaagat gttgagaat tttatcgat 
      gaggacgat gtggttgaa agcttgtac gaacacagc 
      cgtttgaaa aatgaacac agtaaaaag aaaagtgaa 
      aacatcatc gacagcgtt tcggcagag gttgattta 
      atttag
    mRNA sequence
    • >TC016333 RA
      taacaagta ttaaaaaaa tggcaaagg aagcactaa 
      atattcgcc cattcattc cagaatatt tcaaccgca 
      caaccgact tgaacatat tcttcgtgg actggtcct 
      cccttgccg caggtcctt gttacccgg ctgccgtgt 
      ggaacacgc gccatgtgg gcgaatgca cttcccaat 
      tagtcgaac gaataaagg agctaggag ccaaaaaca 
      tccacctga tcggtttta gtttaggcg gccagttaa 
      caaacttcg tcgctaacg cccttcgcc cgtacaaag 
      tttccagga ttacaggac tcgatcctg ccggtcccg 
      gtttcctaa cagcgggcc ccgaaaaca aattagaca 
      aaggcgacg ccgaattcg tcgacgtca tccacacaa 
      acgctttcg tgcaaggca ttgtggagg agagcggcc 
      atgtcgact tttacataa atgggggcg tgatccagc 
      ccggatgtt gggcagaga acagatttt tcgcttgta 
      atcaccacc gggcgccct tgtactttg ccgaatcaa 
      tcacaacac aaatgggtt tttggggct ggccgtgcc 
      cgtcataca ccgagtact taataggga ggtgtccgc 
      ccaaagagc cccagatca taatgggcg agttcgtca 
      acaggacgt ccagtggag tccacttgg ttataaccg 
      attcggttt caccgtttg ctgtgggga aattcacag 
      gaccagcga ttgaaatat tcgtcaagt cggaactaa 
      taagacggg atattttgg aaaagtata aaaaagatg 
      ttgagaatt ttatcgatg aggacgatg tggttgaaa 
      gcttgtacg aacacagcc gtttgaaaa atgaacaca 
      gtaaaaaga aaagtgaaa acatcatcg acagcgttt 
      cggcagagg ttgatttaa tttagtgtg aattgttat 
      attgtggtt acaaataaa gaaataata tattttttt 
      taaacactt tcccaatta ttataccag tgtacgagt 
      atattttgt tgtataact ataaacaaa atttttatt 
      ccttggtaa gttaccaca acattttaa acaacagat 
      cgtacgtat tgagtaata ttttataaa aaaaaaaaa 
      taaataaaa taaagaaaa acttttgtt ttgagcgaa 
      ctaatttat agcgcgctg agatcgtaa aaatagatt 
      tttcctcaa ggcttatca aggtttcta actgcacaa 
      atacacgca attagctgt tccgacttc atcagcgtg 
      tacagtacg tgttcgcat aaaaatggg ggccttaag 
      ccttgaagc cacgaatct gtttgttgg aatattagc 
      acattatca aaagcctta tgaaaaatt aaaacttaa 
      caatagact gataagtaa taactgata accggcaca 
      gttttcaaa attttacca cagttcgta atttacata 
      ataacgtgc tcctgctaa atttacagc acgtaatcg 
      ccgacgtca cagtcaaca aatctatcc cactcgaaa 
      aataaaaag tgtagtata ccgtacagt atttttcgt 
      cgtgaagtg agtgatctt tgaataatg ccaggtatt 
      tttttatat taactaatt atctgtcac tttaacgta 
      ctcaagaac acttttgtt aattatagg cacattaaa 
      cacttctgt cggct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY