Status
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Gene information
General information
TC016332 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451916.1: 21..6368
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC016332
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_02575

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
      Days post injection


      Pupal morphology
      beetle stock: bad (increased lethality),
      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): less than 30%

      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
      Days post injection


      iBeetle screen iB_06973

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016332 RA
        MSAPLQFPA GKLIAPSPI EAMGDGLEH RTHRLFITD 
        PPSNIRFLI DTGAEVSVL PKCFATNSA TPGENVLFA 
        ANNTPIRTY GTRRLTLSL GLRRAFRWE FVVAGIAKP 
        ILGADFLNF HNLLPDLRN RKLVDATTQ LHTRGVVAA 
        GQSEVITTV ARTTPYHEL LAAYPEITR PHTPKPKSQ 
        STVRHHIFT QGPPVAETP RRLSPEKMK VARAEFEFM 
        SEQGWCRPS NSPWASPLH LVPKKVPGQ WRACGDYRK 
        LNSITVPDR YPIPHVQDF AYKLQGKKV FSTIDLVRA 
        YQQIAVADE DIPKTAVCT PFGLFEFTV MCFGLRNAA 
        QTFQRHVNA VLGDLDFCH AYIDDILIA SESPEEHKQ 
        HLKEVFERL RQHNLVVNP AKCVFGVEE VEYLGHRIT 
        PSGCQPLPK KVEAVTNFP KPNTVKELR QFLGMINFY 
        RRFVKNAAQ IQTPLLEYL KNATKNDKR KIEWTPQAE 
        AAFSLCKLE LANAALLAH PMEAGALTL TTDASDNAV 
        GAVLEQKTE GTWQPLGFF SKKLNQAQR NYSTYDREL 
        LAIYEAIKH FRYMVEGRQ FVIRTDHKP LIYAFQQKP 
        DKASPRQLR HLDFIGQFS TEVVHIAGE SNAVADALS 
        RIDEIVTPT ALSPEELQA EQETDPELK DLLKSTTTS 
        LRLKKCTPV GTTASFYCD TSTQFVRPY VPFRLRKAA 
        FKLFHEPAH SGVRATSRA IRQKFVWPR MDDDVKRWT 
        RVCIPCQRS KVTKHTRTE LKKFNVTGT RFEHVHIDI 
        VGPLPEAEG HRYCLTMTD RFTRWPEVT PMRDMSAET 
        VARTFYSTW IARFGCPLR ITTDQGRQF ESHLMTAVS 
        NLFGIKRCR TTAYRPQCN GIIERWHRT IKAAIMCHE 
        NPNWLTILP TILLGLRTA LKQDLQCSP AEMTYGSQL 
        RIPGEFFFS AQTDDGYED PATFVGLLK EHMRKLQPV 
        PATAHDKRK VFVHQELQR CSHVFLRED AVKRPLQQP 
        YTGPYEVLE RRENNVKID VNGKAVLVH LDRLKPAFL 
        AQDVAEPQP PQTVPFSAS RASREDKRS TRPPIRFRD 
        YIPH
      CDS sequence
      • >TC016332 RA
        atgtcggca cccttgcag tttcccgca gggaaacta 
        attgcaccg tccccaatc gaggcaatg ggcgacggt 
        ctcgaacac cggacacat cgcctcttt atcaccgac 
        ccgccgtca aacattcgc ttcctcatc gacaccggc 
        gccgaggtg tcagtgctg cccaaatgt ttcgctaca 
        aactcggca acaccaggg gaaaacgtc ctattcgcc 
        gccaacaac acccctatt cgaacatac ggcacccga 
        cgcctgacc ttaagttta gggctccgc cgggctttt 
        agatgggaa tttgtagtc gccggaatt gccaagcca 
        atcctaggg gcagatttc ctcaacttc cacaactta 
        ttgccagat ctccggaac cggaagcta gtggacgct 
        accacgcag ctacacaca cgtggggtc gtcgccgca 
        ggacaaagt gaggtaatc accacggtg gcgagaaca 
        accccatac cacgaactt ctggccgct tacccagaa 
        attaccaga ccacatacc cccaagccc aaaagccag 
        agcaccgtg cgacaccac attttcacc cagggcccc 
        ccagtggca gaaaccccc cggaggtta tcgccggag 
        aaaatgaaa gtcgcgcga gcagagttc gaatttatg 
        agcgagcaa gggtggtgc cgaccttca aacagcccg 
        tgggcaagc ccgcttcac ctggtgccc aagaaagtt 
        cccggacag tggcgtgca tgtggtgat taccggaaa 
        ctcaacagc atcactgtc ccggacaga tacccaatc 
        ccccacgta caagacttt gcatacaaa ttgcaagga 
        aaaaaagtg ttttcgaca atagaccta gtgcgtgcg 
        taccaacaa atcgcggtc gcggacgag gacattccc 
        aaaaccgca gtgtgcacc ccattcggt ctgttcgaa 
        tttacagtg atgtgcttc ggtctccga aacgcggct 
        cagactttt cagcgtcat gttaacgca gtgctcggt 
        gacctggac ttttgccac gcatacatc gacgatatt 
        ttgatagcc tcggagtcc ccggaggaa cataaacag 
        cacctgaag gaagtattc gaacggctg aggcagcat 
        aacttggtg gtaaatcct gccaagtgc gtgtttggt 
        gtggaggaa gtggaatac ctgggccac cgaatcacc 
        ccctcaggc tgtcaacca ctgcccaaa aaagttgaa 
        gccgtcacc aatttcccc aaacctaat acggtgaag 
        gaactgcgg cagtttttg ggtatgata aatttttat 
        agacgtttt gtgaaaaac gcggcccaa atacaaacc 
        ccgttactc gagtacctc aaaaatgcc accaaaaac 
        gacaagagg aaaatcgaa tggaccccg caagcagaa 
        gctgctttc tcactgtgc aaattggag ctggcaaac 
        gcagccctc ctcgctcac ccaatggag gctggcgcg 
        ctcacgttg acaacagat gcctcggac aacgccgta 
        ggtgccgtt ctcgagcag aagaccgaa ggcacatgg 
        cagcctctg ggcttcttt tcaaagaag ctgaaccaa 
        gctcagagg aactacagc acgtacgac cgcgagctc 
        ctcgccatt tatgaagcc ataaaacat ttccgatac 
        atggtggaa ggtcgacag ttcgtgatc agaacggat 
        cacaaacct ttgatttac gcttttcag caaaaaccg 
        gataaagcg tccccgagg cagttgagg cacctggac 
        ttcatcggt caattttcg accgaagtc gtgcacatc 
        gcgggggaa agtaacgcc gtcgccgac gcgttgtca 
        cgtatcgac gagattgta accccgaca gccctctcg 
        cctgaggag ttgcaagca gagcaagag acagacccc 
        gagctgaag gacttgctc aagtctacc acaacctca 
        ctgagatta aaaaagtgt acgccagtg ggaacgact 
        gcatcattt tactgtgat acgtcgacc caatttgtc 
        aggccttac gtccctttt cgcctgaga aaagcagcg 
        ttcaaactc ttccacgag cccgcgcac tcaggagtt 
        cgagctacc agtcgggct ataagacaa aaattcgtt 
        tggcctaga atggacgac gatgtgaag agatggacc 
        cgagtttgc ataccttgt caacgcagc aaggtaaca 
        aaacacacc cgaaccgag ctaaaaaag ttcaacgtt 
        acagggact cgctttgag catgtacac atcgacatc 
        gtggggcct ttgcccgaa gcagaggga caccgatat 
        tgcctgact atgacagac aggttcacc cgttggccc 
        gaagtcacc cccatgcgc gacatgtcc gccgaaact 
        gtcgcccga actttctac tccacgtgg attgcacga 
        ttcggatgt cctctccga ataaccacc gatcagggc 
        cgtcagttt gagtcccac ctcatgaca gctgtgagc 
        aatcttttc ggcataaaa cgctgcaga accacggcc 
        tacaggccc cagtgcaac ggaatcata gagcgctgg 
        caccgtacc atcaaagcc gccatcatg tgccacgaa 
        aaccccaac tggctaacc atcttaccg acaattctg 
        ctagggcta agaacggcg ctcaaacaa gatctgcaa 
        tgttcgcct gccgagatg acatacggc tcgcagctg 
        agaatccct ggcgaattc ttctttagc gcgcagacc 
        gacgacggg tacgaagac ccagcaaca ttcgtcgga 
        ctactaaaa gagcacatg agaaagttg cagccggta 
        cccgcaaca gcccacgac aagcgaaaa gtgttcgtg 
        caccaggag ttgcagaga tgtagccat gtcttctta 
        agggaagac gccgtgaaa cgaccctta cagcagccg 
        tacacaggc ccctacgaa gtccttgaa aggcgagaa 
        aataacgtc aaaattgac gtcaacggc aaggcagtc 
        ctagttcac ctggatcgc ctcaaacca gcattcttg 
        gcacaggac gtcgctgaa ccccaacct ccacaaacc 
        gtccctttt tcggccagc cgcgcctcc agggaggat 
        aagagatcg acccgacct ccgattaga tttcgcgac 
        tacattccg cattga
      mRNA sequence
      • >TC016332 RA
        gcgccaaat tcaaaatcg actgtagtt cggctggcc 
        atcgcagat gtcgcacac acgtgtagt caattttag 
        cgaagctac ctatattat cggcagaaa ttaacagtg 
        gatgacgca atttaaaaa ttcaagaac acaaaacaa 
        acagacaaa accgtgcaa catggcgac cgtcgaaga 
        gcaagccgc gactattca attgctaca acagcagtt 
        agcggacgc ccaaagact tttaacaga gcggggagc 
        aaacgcaaa tccacctga cgccgcaca gggtaatcc 
        cccagcgtc agggacacc caggcctga agtggagga 
        aatcaatag taccccaaa actgccgcc attttggca 
        caaaaatcc aagcctgtg gtttgtgca agtagaggc 
        ccattttca cgccagccg ggtcaaagc agatctaac 
        caagtactg taacgtggt cgcagcgct ggactctta 
        caccctgca ggcgataga ggacttcct gcggaaccc 
        gccgcaagc tgacaagta cgaacggct gaaggccaa 
        gctcataac gacgtttac ggagtccca ggaacagca 
        gctccgcaa attgttaaa agaaattga gctggggga 
        caaaaagcc atcacgcct cccttgcga gatgagagc 
        gcttgccag ggagcaggt gagcagcga ggtggtgag 
        gacgctgtg gctccaaag gctgccccc acacatcca 
        gttggtcct ttccactat ggacgggat ggatggaga 
        gaaattggc ctccgtagc agacaaact tacggaaat 
        tccaatggc gaatgtaat ggcggtagg cgccgacgc 
        cagcgacgc caacggcat ctgcgccgt gcgcacctc 
        tcacagcgc atccgcgca tcaagcggc agcaacttt 
        aaacgctgg cgccaccaa cgcgcttgc gtctctaca 
        aaaacaggt aactgaatt atcggccaa gctcgacaa 
        actttctgc gacaaaatg gcggcagcg tagccgttc 
        tcaatcacg aagccgcaa ccctcttca acagcaaca 
        aaatggcga caatgtctg tttctacca caataaatt 
        tggtgctga agccaggaa atgtcggca cccttgcag 
        tttcccgca gggaaacta attgcaccg tccccaatc 
        gaggcaatg ggcgacggt ctcgaacac cggacacat 
        cgcctcttt atcaccgac ccgccgtca aacattcgc 
        ttcctcatc gacaccggc gccgaggtg tcagtgctg 
        cccaaatgt ttcgctaca aactcggca acaccaggg 
        gaaaacgtc ctattcgcc gccaacaac acccctatt 
        cgaacatac ggcacccga cgcctgacc ttaagttta 
        gggctccgc cgggctttt agatgggaa tttgtagtc 
        gccggaatt gccaagcca atcctaggg gcagatttc 
        ctcaacttc cacaactta ttgccagat ctccggaac 
        cggaagcta gtggacgct accacgcag ctacacaca 
        cgtggggtc gtcgccgca ggacaaagt gaggtaatc 
        accacggtg gcgagaaca accccatac cacgaactt 
        ctggccgct tacccagaa attaccaga ccacatacc 
        cccaagccc aaaagccag agcaccgtg cgacaccac 
        attttcacc cagggcccc ccagtggca gaaaccccc 
        cggaggtta tcgccggag aaaatgaaa gtcgcgcga 
        gcagagttc gaatttatg agcgagcaa gggtggtgc 
        cgaccttca aacagcccg tgggcaagc ccgcttcac 
        ctggtgccc aagaaagtt cccggacag tggcgtgca 
        tgtggtgat taccggaaa ctcaacagc atcactgtc 
        ccggacaga tacccaatc ccccacgta caagacttt 
        gcatacaaa ttgcaagga aaaaaagtg ttttcgaca 
        atagaccta gtgcgtgcg taccaacaa atcgcggtc 
        gcggacgag gacattccc aaaaccgca gtgtgcacc 
        ccattcggt ctgttcgaa tttacagtg atgtgcttc 
        ggtctccga aacgcggct cagactttt cagcgtcat 
        gttaacgca gtgctcggt gacctggac ttttgccac 
        gcatacatc gacgatatt ttgatagcc tcggagtcc 
        ccggaggaa cataaacag cacctgaag gaagtattc 
        gaacggctg aggcagcat aacttggtg gtaaatcct 
        gccaagtgc gtgtttggt gtggaggaa gtggaatac 
        ctgggccac cgaatcacc ccctcaggc tgtcaacca 
        ctgcccaaa aaagttgaa gccgtcacc aatttcccc 
        aaacctaat acggtgaag gaactgcgg cagtttttg 
        ggtatgata aatttttat agacgtttt gtgaaaaac 
        gcggcccaa atacaaacc ccgttactc gagtacctc 
        aaaaatgcc accaaaaac gacaagagg aaaatcgaa 
        tggaccccg caagcagaa gctgctttc tcactgtgc 
        aaattggag ctggcaaac gcagccctc ctcgctcac 
        ccaatggag gctggcgcg ctcacgttg acaacagat 
        gcctcggac aacgccgta ggtgccgtt ctcgagcag 
        aagaccgaa ggcacatgg cagcctctg ggcttcttt 
        tcaaagaag ctgaaccaa gctcagagg aactacagc 
        acgtacgac cgcgagctc ctcgccatt tatgaagcc 
        ataaaacat ttccgatac atggtggaa ggtcgacag 
        ttcgtgatc agaacggat cacaaacct ttgatttac 
        gcttttcag caaaaaccg gataaagcg tccccgagg 
        cagttgagg cacctggac ttcatcggt caattttcg 
        accgaagtc gtgcacatc gcgggggaa agtaacgcc 
        gtcgccgac gcgttgtca cgtatcgac gagattgta 
        accccgaca gccctctcg cctgaggag ttgcaagca 
        gagcaagag acagacccc gagctgaag gacttgctc 
        aagtctacc acaacctca ctgagatta aaaaagtgt 
        acgccagtg ggaacgact gcatcattt tactgtgat 
        acgtcgacc caatttgtc aggccttac gtccctttt 
        cgcctgaga aaagcagcg ttcaaactc ttccacgag 
        cccgcgcac tcaggagtt cgagctacc agtcgggct 
        ataagacaa aaattcgtt tggcctaga atggacgac 
        gatgtgaag agatggacc cgagtttgc ataccttgt 
        caacgcagc aaggtaaca aaacacacc cgaaccgag 
        ctaaaaaag ttcaacgtt acagggact cgctttgag 
        catgtacac atcgacatc gtggggcct ttgcccgaa 
        gcagaggga caccgatat tgcctgact atgacagac 
        aggttcacc cgttggccc gaagtcacc cccatgcgc 
        gacatgtcc gccgaaact gtcgcccga actttctac 
        tccacgtgg attgcacga ttcggatgt cctctccga 
        ataaccacc gatcagggc cgtcagttt gagtcccac 
        ctcatgaca gctgtgagc aatcttttc ggcataaaa 
        cgctgcaga accacggcc tacaggccc cagtgcaac 
        ggaatcata gagcgctgg caccgtacc atcaaagcc 
        gccatcatg tgccacgaa aaccccaac tggctaacc 
        atcttaccg acaattctg ctagggcta agaacggcg 
        ctcaaacaa gatctgcaa tgttcgcct gccgagatg 
        acatacggc tcgcagctg agaatccct ggcgaattc 
        ttctttagc gcgcagacc gacgacggg tacgaagac 
        ccagcaaca ttcgtcgga ctactaaaa gagcacatg 
        agaaagttg cagccggta cccgcaaca gcccacgac 
        aagcgaaaa gtgttcgtg caccaggag ttgcagaga 
        tgtagccat gtcttctta agggaagac gccgtgaaa 
        cgaccctta cagcagccg tacacaggc ccctacgaa 
        gtccttgaa aggcgagaa aataacgtc aaaattgac 
        gtcaacggc aaggcagtc ctagttcac ctggatcgc 
        ctcaaacca gcattcttg gcacaggac gtcgctgaa 
        ccccaacct ccacaaacc gtccctttt tcggccagc 
        cgcgcctcc agggaggat aagagatcg acccgacct 
        ccgattaga tttcgcgac tacattccg cattgacag 
        cgtccatgc acctagtgt aatgtagcc actggaggg 
        ggagtgatg tggcgccct ctggggagg ggacgtcag 
        ttctcaagt cccgcaaaa acaaaaacc gcgtgtcgc 
        gtaatcagc ctccatttg ttattttat atggttgta 
        cgaataccg tgtgtgaat aaaaccttg tgtaaaag
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