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Gene information
General information
TC000998 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 9532085..9541000
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000998
    iBeetle screen iB_02574

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Fertility
    ovary shape irregular - Number of animals showing the phenotype: 4
    tropharium potentially degenerated - Number of animals showing the phenotype: 4

    egg chamber mostly not present - Number of animals showing the phenotype: 4

    egg chamber potentially degenerated - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    Pupal morphology
    legs potentially shortened - Number of animals showing the phenotype: 8
    iBeetle screen iB_02653

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    tropharium structure irregular - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000998 RA
      MPKSMNVRV TTMDAELEF AIQQTTSGK QLFDQVVKT 
IGLREVWFF GLQYTDSKG DLTWIKLYK KVMSQDVKK 
ENPLQFKFR AKFYPEDVA EELIQDITL RLFYLQVKN 
SILSDEIYC PPETSVLLA SYAVQARYG DYCKAVHTS 
GFLSNDRLL PQRVMDQHK MSKEEWEQS IMTWWKEHR 
GMLREDTMM EYLKIAQDL EMYGVNYFE IRNKKGTEL 
YLGVDALGL NIYEKDDRL TPKIGFPWS EIRNISFND 
RKFIIKPID KKAPDFVFF APRVRINKR ILSLCMGNH 
ELYMRRRKP DTIDVQQMK AQAREEKLA KQQQREKLQ 
LEIAARERA EKKQQEYED RIKAMQEEM ERSQANLLE 
AQEMIRRLE EQLKQLQAA KEELEKRQN ELQAMMERL 
EESKNMEAA ERQKLEEEI QAKQLEVQR IQDEVTAKD 
NETKRLQEE VENARRKEE ELKAQQMAN ATKGHLEEN 
NHDEDDEMV NGDVSKDLT TDENIVDPV EDRRTLAER 
NERLHDQLL ALKKDLAQS RDETKETAM DKIHRENVR 
QGRDKYKTL REIRKGNTK RRVDQFENM
    CDS sequence
    • >TC000998 RA
      atgccgaaa tcgatgaat gtcagggtg accactatg 
gatgcggaa ctcgagttc gcaatccag caaacaaca 
tccggaaaa caactcttc gatcaagta gttaaaaca 
atcggattg agagaagtt tggtttttc ggactacag 
tacaccgat tctaagggt gatctaaca tggatcaag 
ctgtacaaa aaggtgatg agccaagat gtgaagaaa 
gaaaatcca cttcaattc aagttccgt gccaaattt 
tatcccgaa gatgtcgcc gaagaatta attcaagac 
atcactttg cgtttattt tatttacaa gttaaaaac 
tctatctta tctgatgag atttattgt cctcctgaa 
acgtccgtc cttttagct tcctatgcc gttcaagcg 
agatacggc gattattgt aaggccgtt catacctcc 
ggattcctt tcaaacgac agactttta ccacaaaga 
gtcatggac cagcacaag atgtcgaaa gaggagtgg 
gagcagagc attatgacc tggtggaag gagcatcgt 
ggtatgttg cgcgaagac accatgatg gaatacctg 
aaaatagcc caggacttg gaaatgtac ggtgtgaac 
tactttgaa attcgcaac aaaaaaggc accgaactg 
tatttgggc gtggacgcc ttgggtctg aacatttac 
gaaaaagac gaccgacta acgcccaaa atcggcttt 
ccctggtcg gaaatccgc aatatttcg ttcaacgac 
cgcaaattc attataaag ccaatcgat aagaaggcc 
cccgatttc gtatttttc gcgcctcgc gttcgaata 
aacaaacga attttatct ctatgcatg ggtaatcac 
gagttgtac atgcgacgt cggaagccc gacaccata 
gacgtgcag cagatgaaa gcccaagct cgtgaagaa 
aaactagcc aaacaacaa caaagggaa aaactccaa 
ttggagatt gcggcgaga gaacgcgcc gagaagaaa 
caacaagag tatgaagat aggattaaa gcaatgcaa 
gaggagatg gagcgaagc caagccaat ctcctcgag 
gcacaagag atgatccgg cgcctggag gagcaactg 
aagcagttg caagcggcc aaggaggag ctggagaag 
aggcagaac gagctgcag gcgatgatg gagcgtttg 
gaggaaagc aagaacatg gaagctgcc gaacgccaa 
aagttggag gaggagatt caagccaaa cagttggag 
gtgcagagg atccaagac gaggtgact gcgaaggac 
aatgaaact aagcgtctg caagaggag gtcgagaac 
gcgcgtcgc aaggaagag gagctgaag gcccagcaa 
atggccaat gcgaccaaa ggccacctg gaggagaac 
aaccacgat gaggacgat gagatggtc aatggggac 
gtgagcaag gatttgacc acggatgag aacattgtg 
gatcctgtg gaagacaga cgcactctt gctgagcgc 
aatgagcga ctccatgat caacttctg gctttgaag 
aaagacctc gcccagtcg cgggacgag acgaaggaa 
accgccatg gacaagatc catcgagag aacgtgcgc 
cagggcaga gataaatac aaaactctg cgtgagata 
cgtaaaggt aacactaag cgtcgcgtg gaccaattc 
gaaaatatg taa
    mRNA sequence
    • >TC000998 RA
      tttggggta gaacccttg gttttgtgg tcgtttggt 
agatggcag tatatcgcg catttttgg tcctctggt 
ggcaaagtc ggaactaaa cgatacaca atttgctga 
ttcttgaca atttatttt ccgataagt tgtcgtgtc 
gtgtgtgga agggtgtgt gcccagaaa aaccccgcg 
tgtgctact taatgtggt ggctacaag agtgttaat 
cttcaaaag atatatttc ttagttttt tggttcgag 
acaagactg ccaacatgc cgaaatcga tgaatgtca 
gggtgacca ctatggatg cggaactcg agttcgcaa 
tccagcaaa caacatccg gaaaacaac tcttcgatc 
aagtagtta aaacaatcg gattgagag aagtttggt 
ttttcggac tacagtaca ccgattcta agggtgatc 
taacatgga tcaagctgt acaaaaagg tgatgagcc 
aagatgtga agaaagaaa atccacttc aattcaagt 
tccgtgcca aattttatc ccgaagatg tcgccgaag 
aattaattc aagacatca ctttgcgtt tattttatt 
tacaagtta aaaactcta tcttatctg atgagattt 
attgtcctc ctgaaacgt ccgtccttt tagcttcct 
atgccgttc aagcgagat acggcgatt attgtaagg 
ccgttcata cctccggat tcctttcaa acgacagac 
ttttaccac aaagagtca tggaccagc acaagatgt 
cgaaagagg agtgggagc agagcatta tgacctggt 
ggaaggagc atcgtggta tgttgcgcg aagacacca 
tgatggaat acctgaaaa tagcccagg acttggaaa 
tgtacggtg tgaactact ttgaaattc gcaacaaaa 
aaggcaccg aactgtatt tgggcgtgg acgccttgg 
gtctgaaca tttacgaaa aagacgacc gactaacgc 
ccaaaatcg gctttccct ggtcggaaa tccgcaata 
tttcgttca acgaccgca aattcatta taaagccaa 
tcgataaga aggcccccg atttcgtat ttttcgcgc 
ctcgcgttc gaataaaca aacgaattt tatctctat 
gcatgggta atcacgagt tgtacatgc gacgtcgga 
agcccgaca ccatagacg tgcagcaga tgaaagccc 
aagctcgtg aagaaaaac tagccaaac aacaacaaa 
gggaaaaac tccaattgg agattgcgg cgagagaac 
gcgccgaga agaaacaac aagagtatg aagatagga 
ttaaagcaa tgcaagagg agatggagc gaagccaag 
ccaatctcc tcgaggcac aagagatga tccggcgcc 
tggaggagc aactgaagc agttgcaag cggccaagg 
aggagctgg agaagaggc agaacgagc tgcaggcga 
tgatggagc gtttggagg aaagcaaga acatggaag 
ctgccgaac gccaaaagt tggaggagg agattcaag 
ccaaacagt tggaggtgc agaggatcc aagacgagg 
tgactgcga aggacaatg aaactaagc gtctgcaag 
aggaggtcg agaacgcgc gtcgcaagg aagaggagc 
tgaaggccc agcaaatgg ccaatgcga ccaaaggcc 
acctggagg agaacaacc acgatgagg acgatgaga 
tggtcaatg gggacgtga gcaaggatt tgaccacgg 
atgagaaca ttgtggatc ctgtggaag acagacgca 
ctcttgctg agcgcaatg agcgactcc atgatcaac 
ttctggctt tgaagaaag acctcgccc agtcgcggg 
acgagacga aggaaaccg ccatggaca agatccatc 
gagagaacg tgcgccagg gcagagata aatacaaaa 
ctctgcgtg agatacgta aaggtaaca ctaagcgtc 
gcgtggacc aattcgaaa atatgtaaa tcacaatca 
aaatcaaaa ttccatatt caaactttc aatgctcaa 
tacgagatc atgttaaat ttaagtttt ggtttactt 
acgtctaag tatgatttt tttccaatg taagataaa 
aagtgctca tttaagtgg ccttagact ttcaaatct 
tagttacac tattttcat tcttgtttt gttgcaaat 
tattttgtt agaattgtg atatgatat gagaggaga 
aaaaaacag atctgttaa ttcactttc tttacattt 
aaatcactt aaacgcatt ccacgtaat ggtagtttt 
tcttctaat tttagtgcc atttggtta acgggctac 
acaaaaata atctttgtt ttttgaaat tcgaattga 
ttctaagat cagctaaat tttgtacac ttttattgg 
cctggtgct tccttattg tgtaaataa aagttatac 
aattcgttt tttctactt actgtattt ttatcgtgg 
ttaaaaaga aggttttta caatatttt ttctacaat 
acttaatct tttacctgt tacatatcg taacaataa 
agtaatgta cataacatt tatttttta aagaccatg 
atgccttct agataggtt tggatgata tttacactg 
tagtatgtc agatgggtg aggaaaaac cgttcttca 
aatggtttt ttaggaaag aaattaact aatccttat 
ttaaataaa ccgcagata tcaaacgtc tgttttatt 
atacattta tataatata tatttatct tgcgatcac 
ttgtatatt gtacatcct aaaactacg tcgctagtt 
tttaacatt tgtattctt cgtagttta tgagttatg 
tactactag tataattaa taaatatta tttggaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY