General information | |||
TC000998 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 9532085..9541000 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC000998 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02574 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
tropharium potentially degenerated - Number of animals showing the phenotype: 4
egg chamber mostly not present - Number of animals showing the phenotype: 4
egg chamber potentially degenerated - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (11 dpi)
pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (16 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02653 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
tropharium structure irregular - Number of animals showing the phenotype: 4
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 6 (11 dpi)
- >TC000998 RA
MPKSMNVRV TTMDAELEF AIQQTTSGK QLFDQVVKT IGLREVWFF GLQYTDSKG DLTWIKLYK KVMSQDVKK ENPLQFKFR AKFYPEDVA EELIQDITL RLFYLQVKN SILSDEIYC PPETSVLLA SYAVQARYG DYCKAVHTS GFLSNDRLL PQRVMDQHK MSKEEWEQS IMTWWKEHR GMLREDTMM EYLKIAQDL EMYGVNYFE IRNKKGTEL YLGVDALGL NIYEKDDRL TPKIGFPWS EIRNISFND RKFIIKPID KKAPDFVFF APRVRINKR ILSLCMGNH ELYMRRRKP DTIDVQQMK AQAREEKLA KQQQREKLQ LEIAARERA EKKQQEYED RIKAMQEEM ERSQANLLE AQEMIRRLE EQLKQLQAA KEELEKRQN ELQAMMERL EESKNMEAA ERQKLEEEI QAKQLEVQR IQDEVTAKD NETKRLQEE VENARRKEE ELKAQQMAN ATKGHLEEN NHDEDDEMV NGDVSKDLT TDENIVDPV EDRRTLAER NERLHDQLL ALKKDLAQS RDETKETAM DKIHRENVR QGRDKYKTL REIRKGNTK RRVDQFENM
- >TC000998 RA
atgccgaaa tcgatgaat gtcagggtg accactatg gatgcggaa ctcgagttc gcaatccag caaacaaca tccggaaaa caactcttc gatcaagta gttaaaaca atcggattg agagaagtt tggtttttc ggactacag tacaccgat tctaagggt gatctaaca tggatcaag ctgtacaaa aaggtgatg agccaagat gtgaagaaa gaaaatcca cttcaattc aagttccgt gccaaattt tatcccgaa gatgtcgcc gaagaatta attcaagac atcactttg cgtttattt tatttacaa gttaaaaac tctatctta tctgatgag atttattgt cctcctgaa acgtccgtc cttttagct tcctatgcc gttcaagcg agatacggc gattattgt aaggccgtt catacctcc ggattcctt tcaaacgac agactttta ccacaaaga gtcatggac cagcacaag atgtcgaaa gaggagtgg gagcagagc attatgacc tggtggaag gagcatcgt ggtatgttg cgcgaagac accatgatg gaatacctg aaaatagcc caggacttg gaaatgtac ggtgtgaac tactttgaa attcgcaac aaaaaaggc accgaactg tatttgggc gtggacgcc ttgggtctg aacatttac gaaaaagac gaccgacta acgcccaaa atcggcttt ccctggtcg gaaatccgc aatatttcg ttcaacgac cgcaaattc attataaag ccaatcgat aagaaggcc cccgatttc gtatttttc gcgcctcgc gttcgaata aacaaacga attttatct ctatgcatg ggtaatcac gagttgtac atgcgacgt cggaagccc gacaccata gacgtgcag cagatgaaa gcccaagct cgtgaagaa aaactagcc aaacaacaa caaagggaa aaactccaa ttggagatt gcggcgaga gaacgcgcc gagaagaaa caacaagag tatgaagat aggattaaa gcaatgcaa gaggagatg gagcgaagc caagccaat ctcctcgag gcacaagag atgatccgg cgcctggag gagcaactg aagcagttg caagcggcc aaggaggag ctggagaag aggcagaac gagctgcag gcgatgatg gagcgtttg gaggaaagc aagaacatg gaagctgcc gaacgccaa aagttggag gaggagatt caagccaaa cagttggag gtgcagagg atccaagac gaggtgact gcgaaggac aatgaaact aagcgtctg caagaggag gtcgagaac gcgcgtcgc aaggaagag gagctgaag gcccagcaa atggccaat gcgaccaaa ggccacctg gaggagaac aaccacgat gaggacgat gagatggtc aatggggac gtgagcaag gatttgacc acggatgag aacattgtg gatcctgtg gaagacaga cgcactctt gctgagcgc aatgagcga ctccatgat caacttctg gctttgaag aaagacctc gcccagtcg cgggacgag acgaaggaa accgccatg gacaagatc catcgagag aacgtgcgc cagggcaga gataaatac aaaactctg cgtgagata cgtaaaggt aacactaag cgtcgcgtg gaccaattc gaaaatatg taa
- >TC000998 RA
tttggggta gaacccttg gttttgtgg tcgtttggt agatggcag tatatcgcg catttttgg tcctctggt ggcaaagtc ggaactaaa cgatacaca atttgctga ttcttgaca atttatttt ccgataagt tgtcgtgtc gtgtgtgga agggtgtgt gcccagaaa aaccccgcg tgtgctact taatgtggt ggctacaag agtgttaat cttcaaaag atatatttc ttagttttt tggttcgag acaagactg ccaacatgc cgaaatcga tgaatgtca gggtgacca ctatggatg cggaactcg agttcgcaa tccagcaaa caacatccg gaaaacaac tcttcgatc aagtagtta aaacaatcg gattgagag aagtttggt ttttcggac tacagtaca ccgattcta agggtgatc taacatgga tcaagctgt acaaaaagg tgatgagcc aagatgtga agaaagaaa atccacttc aattcaagt tccgtgcca aattttatc ccgaagatg tcgccgaag aattaattc aagacatca ctttgcgtt tattttatt tacaagtta aaaactcta tcttatctg atgagattt attgtcctc ctgaaacgt ccgtccttt tagcttcct atgccgttc aagcgagat acggcgatt attgtaagg ccgttcata cctccggat tcctttcaa acgacagac ttttaccac aaagagtca tggaccagc acaagatgt cgaaagagg agtgggagc agagcatta tgacctggt ggaaggagc atcgtggta tgttgcgcg aagacacca tgatggaat acctgaaaa tagcccagg acttggaaa tgtacggtg tgaactact ttgaaattc gcaacaaaa aaggcaccg aactgtatt tgggcgtgg acgccttgg gtctgaaca tttacgaaa aagacgacc gactaacgc ccaaaatcg gctttccct ggtcggaaa tccgcaata tttcgttca acgaccgca aattcatta taaagccaa tcgataaga aggcccccg atttcgtat ttttcgcgc ctcgcgttc gaataaaca aacgaattt tatctctat gcatgggta atcacgagt tgtacatgc gacgtcgga agcccgaca ccatagacg tgcagcaga tgaaagccc aagctcgtg aagaaaaac tagccaaac aacaacaaa gggaaaaac tccaattgg agattgcgg cgagagaac gcgccgaga agaaacaac aagagtatg aagatagga ttaaagcaa tgcaagagg agatggagc gaagccaag ccaatctcc tcgaggcac aagagatga tccggcgcc tggaggagc aactgaagc agttgcaag cggccaagg aggagctgg agaagaggc agaacgagc tgcaggcga tgatggagc gtttggagg aaagcaaga acatggaag ctgccgaac gccaaaagt tggaggagg agattcaag ccaaacagt tggaggtgc agaggatcc aagacgagg tgactgcga aggacaatg aaactaagc gtctgcaag aggaggtcg agaacgcgc gtcgcaagg aagaggagc tgaaggccc agcaaatgg ccaatgcga ccaaaggcc acctggagg agaacaacc acgatgagg acgatgaga tggtcaatg gggacgtga gcaaggatt tgaccacgg atgagaaca ttgtggatc ctgtggaag acagacgca ctcttgctg agcgcaatg agcgactcc atgatcaac ttctggctt tgaagaaag acctcgccc agtcgcggg acgagacga aggaaaccg ccatggaca agatccatc gagagaacg tgcgccagg gcagagata aatacaaaa ctctgcgtg agatacgta aaggtaaca ctaagcgtc gcgtggacc aattcgaaa atatgtaaa tcacaatca aaatcaaaa ttccatatt caaactttc aatgctcaa tacgagatc atgttaaat ttaagtttt ggtttactt acgtctaag tatgatttt tttccaatg taagataaa aagtgctca tttaagtgg ccttagact ttcaaatct tagttacac tattttcat tcttgtttt gttgcaaat tattttgtt agaattgtg atatgatat gagaggaga aaaaaacag atctgttaa ttcactttc tttacattt aaatcactt aaacgcatt ccacgtaat ggtagtttt tcttctaat tttagtgcc atttggtta acgggctac acaaaaata atctttgtt ttttgaaat tcgaattga ttctaagat cagctaaat tttgtacac ttttattgg cctggtgct tccttattg tgtaaataa aagttatac aattcgttt tttctactt actgtattt ttatcgtgg ttaaaaaga aggttttta caatatttt ttctacaat acttaatct tttacctgt tacatatcg taacaataa agtaatgta cataacatt tatttttta aagaccatg atgccttct agataggtt tggatgata tttacactg tagtatgtc agatgggtg aggaaaaac cgttcttca aatggtttt ttaggaaag aaattaact aatccttat ttaaataaa ccgcagata tcaaacgtc tgttttatt atacattta tataatata tatttatct tgcgatcac ttgtatatt gtacatcct aaaactacg tcgctagtt tttaacatt tgtattctt cgtagttta tgagttatg tactactag tataattaa taaatatta tttggaaaa
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
