Status
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Gene information
General information
TC016323 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 847460..857501
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016323
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02573

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: - Number of animals showing the phenotype: 1
    ovary without detectable defects - Number of animals showing the phenotype: 3
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment:
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    Comment: most of the embryos seem to die during late embryonic development. for many of them ist is not clearly visible if they already secrete a cuticle.
    larval musculature potentially fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: fluorescence is lower, either due to not fully developed musculature or to lethality
    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature partially spotted - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva cuticle thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (22 dpi)
    Days post injection


    Adult morphology
    adult cuticle colour darker - Number of animals showing the phenotype: 4


    pronotum surface irregular - Number of animals showing the phenotype: 2

    Comment: sensory organ distribution is irregular, some patches without any sensory organs

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016323 RA
      MGLLTEGTP LTWEETKKL AEHVRQHGI IQFINLYKR 
      LKDRQGDVL KWGDEVEYI LVKFNDEKK VARVSLRAD 
      EILTELNKK ELENPETVK SLWRPEYGA YMIEGTPGK 
      PYGGLLAHF NIVEANMRH RREEASQLL KADECLMTI 
      TSFPRLGCP NFTDPATKP TPEEGATRS LFFPDDAVF 
      SGHPRFKTL SRNIRQRRG EKVAINLPI YRDKNTQIP 
      VDDSYKYSS AALPDHVYM DAMGFGMGC CCLQLTFQA 
      CNITEARTL YDQLTPLCP IMLALTAAS PLHRGFVTD 
      VDCRWNVIS CSVDCRTKE ERGLAPLKN DKFVIKKSR 
      YDSIDSYLS PQGEKFNDV PLIYDPKIY QQLVDNDID 
      HLLAQHIAH LFIRDSVSL FSEKINQND EEDTDHFEN 
      IQSTNWQTM RFKPPPPNS TIGWRVEFR PCEVQITDF 
      ENAAIVCFV VLLTRVILS YNLDFLIPI SRVDENMQN 
      AQKRNACME QKFWFRKEV TTQISPPEA NECCKKGTT 
      NCEPKNCDV YTCMTVNEI INGKDGEFP GLIPLINTY 
      LSGMDVDAD THCTIQQYL KFIQRRASG EILTTASWL 
      RKFVTEHPS YNKDSKVTE EINYDLLQT IKGIQSGEL 
      SCPELLGYS TMSKTKENI PSALSLSNC
    CDS sequence
    • >TC016323 RA
      atgggtttg ctaactgaa ggaacacct ctaacttgg 
      gaagaaacg aaaaaattg gccgaacac gtgcgtcaa 
      cacggcatt atccagttt ataaacttg tacaagcgg 
      ctgaaagac cggcaagga gatgtcttg aaatggggc 
      gacgaggtg gagtatatt cttgttaag ttcaacgat 
      gagaaaaaa gtggccaga gttagctta agggcggat 
      gaaatccta actgaattg aataagaaa gaactggaa 
      aacccagag accgtcaag tcgctgtgg aggcccgaa 
      tatggggct tacatgatt gagggcact cctgggaag 
      ccttatggg ggcttgttg gcccatttc aacattgtg 
      gaagccaac atgcgacac aggagggag gaagcgtcg 
      caattgctc aaagccgat gaatgccta atgaccatt 
      actagtttt cccagactg ggctgtccg aacttcacc 
      gaccctgcc actaaacca acaccggaa gagggcgcc 
      acccgttcg ttgtttttc cccgacgat gcggttttt 
      tcgggccat ccccgtttt aagaccttg tcacgcaac 
      attcgtcaa agacgtggt gaaaaagtt gccatcaat 
      ttaccaatc tacagggat aaaaataca caaatacca 
      gtggacgat tcctacaaa tactcttca gccgctttg 
      cctgaccat gtctatatg gacgcgatg ggcttcggt 
      atgggatgt tgctgtttg cagctgact ttccaagct 
      tgcaacatc acagaagcc aggacttta tacgaccaa 
      ttgacacct ctgtgccca attatgttg gctttaaca 
      gcagcatca cctcttcac cgcggcttt gtcacggat 
      gttgactgt cgatggaac gtgatttcg tgttctgtt 
      gattgtcgc accaaggaa gaacgcggt ttagcaccg 
      ctcaaaaac gataaattt gtgataaaa aagtcgcgt 
      tatgattcg attgattcg tatctgtcg ccacaaggc 
      gagaagttc aatgatgtg cccttgata tacgatccc 
      aaaatatac caacagttg gttgataat gacattgac 
      catttgttg gctcaacat attgctcat ctttttatt 
      agagactcg gtatccctg ttttcggaa aaaatcaac 
      caaaacgat gaagaggac accgaccat tttgagaat 
      atacagtcg accaattgg caaactatg cgctttaaa 
      ccgccacca cccaactca actattggt tggagggtt 
      gagttcaga ccttgtgaa gtccaaata accgacttc 
      gagaatgct gccattgtt tgttttgta gttttgtta 
      acacgtgtg attttatcc tacaatttg gactttcta 
      attccgata agcagggtg gacgagaat atgcaaaac 
      gcgcaaaaa aggaacgct tgcatggaa caaaaattc 
      tggtttcga aaggaagtt acgactcaa attagtcca 
      ccggaagcc aatgagtgc tgtaagaag ggtacaaca 
      aactgtgag cctaaaaac tgtgatgtt tacacttgt 
      atgactgtt aatgaaatt ataaacgga aaggacggc 
      gaatttcca ggtttgatt cctttaatt aatacgtat 
      ttaagtgga atggatgtt gatgccgat actcattgt 
      acaattcaa cagtatttg aagtttata caacgtcga 
      gcttcaggt gaaatactc accacagct tcgtggtta 
      cgtaaattc gtcactgaa catccttca tacaacaaa 
      gattcgaaa gtaacagaa gagatcaat tacgatttg 
      ttgcaaaca atcaaaggg atccaatct ggagaactg 
      tcttgtccc gaattactg ggttactct acaatgtcc 
      aaaacaaag gaaaatatt ccttcagct ttgtctttg 
      tcaaactgt taa
    mRNA sequence
    • >TC016323 RA
      ttgctcgcg agatgtcac tgacatctt taattaaac 
      aaaaattaa aaagtcatc gcgtgcgct tgcgcagtg 
      cccaaggtc acctacgtc acgttgttc tgtcaacct 
      aatcactga gtggtcaat gttagtgca gaatttaaa 
      cgaaggaat agtttagat tagatatac agttcccca 
      tttctctgt atcttatct aattgtgta agaacgtgt 
      aacttcact tgataaatt cgcgtagtt tttgtctgt 
      caccctagt ttcaaattg actcttcaa agagaatct 
      cgcattttt tgtttgaaa tttgcgaaa aaaacaact 
      ttttcttta accaacgtc ctaataaac catcaacgc 
      attacatgc tacaagttt caaagtaaa tcataaagt 
      gacagttcg gtgaattaa aaaaaagtg atgggtttg 
      ctaactgaa ggaacacct ctaacttgg gaagaaacg 
      aaaaaattg gccgaacac gtgcgtcaa cacggcatt 
      atccagttt ataaacttg tacaagcgg ctgaaagac 
      cggcaagga gatgtcttg aaatggggc gacgaggtg 
      gagtatatt cttgttaag ttcaacgat gagaaaaaa 
      gtggccaga gttagctta agggcggat gaaatccta 
      actgaattg aataagaaa gaactggaa aacccagag 
      accgtcaag tcgctgtgg aggcccgaa tatggggct 
      tacatgatt gagggcact cctgggaag ccttatggg 
      ggcttgttg gcccatttc aacattgtg gaagccaac 
      atgcgacac aggagggag gaagcgtcg caattgctc 
      aaagccgat gaatgccta atgaccatt actagtttt 
      cccagactg ggctgtccg aacttcacc gaccctgcc 
      actaaacca acaccggaa gagggcgcc acccgttcg 
      ttgtttttc cccgacgat gcggttttt tcgggccat 
      ccccgtttt aagaccttg tcacgcaac attcgtcaa 
      agacgtggt gaaaaagtt gccatcaat ttaccaatc 
      tacagggat aaaaataca caaatacca gtggacgat 
      tcctacaaa tactcttca gccgctttg cctgaccat 
      gtctatatg gacgcgatg ggcttcggt atgggatgt 
      tgctgtttg cagctgact ttccaagct tgcaacatc 
      acagaagcc aggacttta tacgaccaa ttgacacct 
      ctgtgccca attatgttg gctttaaca gcagcatca 
      cctcttcac cgcggcttt gtcacggat gttgactgt 
      cgatggaac gtgatttcg tgttctgtt gattgtcgc 
      accaaggaa gaacgcggt ttagcaccg ctcaaaaac 
      gataaattt gtgataaaa aagtcgcgt tatgattcg 
      attgattcg tatctgtcg ccacaaggc gagaagttc 
      aatgatgtg cccttgata tacgatccc aaaatatac 
      caacagttg gttgataat gacattgac catttgttg 
      gctcaacat attgctcat ctttttatt agagactcg 
      gtatccctg ttttcggaa aaaatcaac caaaacgat 
      gaagaggac accgaccat tttgagaat atacagtcg 
      accaattgg caaactatg cgctttaaa ccgccacca 
      cccaactca actattggt tggagggtt gagttcaga 
      ccttgtgaa gtccaaata accgacttc gagaatgct 
      gccattgtt tgttttgta gttttgtta acacgtgtg 
      attttatcc tacaatttg gactttcta attccgata 
      agcagggtg gacgagaat atgcaaaac gcgcaaaaa 
      aggaacgct tgcatggaa caaaaattc tggtttcga 
      aaggaagtt acgactcaa attagtcca ccggaagcc 
      aatgagtgc tgtaagaag ggtacaaca aactgtgag 
      cctaaaaac tgtgatgtt tacacttgt atgactgtt 
      aatgaaatt ataaacgga aaggacggc gaatttcca 
      ggtttgatt cctttaatt aatacgtat ttaagtgga 
      atggatgtt gatgccgat actcattgt acaattcaa 
      cagtatttg aagtttata caacgtcga gcttcaggt 
      gaaatactc accacagct tcgtggtta cgtaaattc 
      gtcactgaa catccttca tacaacaaa gattcgaaa 
      gtaacagaa gagatcaat tacgatttg ttgcaaaca 
      atcaaaggg atccaatct ggagaactg tcttgtccc 
      gaattactg ggttactct acaatgtcc aaaacaaag 
      gaaaatatt ccttcagct ttgtctttg tcaaactgt 
      taaaaacac gtttgttaa tccgtactt aaaaataca 
      ctcgatgca aaattaggt aaacgttaa acatgatga 
      aaataaaat caccattac tttgtcgtc ggacaagtt 
      attaacgtc atgtcagaa tttttgctg atactaaag 
      attgtgaaa aaatcaagt gctaattta acggttgtg 
      atggtatta aatgccgat ttacactat tgtttaccg 
      aaaaactat ttttttttt aagaattgc ttgctttcc 
      ctttacggt agtagttcc ttcgatgac gatcagtgt 
      taaaaaaaa ttattaaaa tttcagacg cgatgatcc 
      ttattaaaa tcttggtct agttccatc gaacttgtt 
      gctaatttt ttttcataa tcctttagt atcacagaa 
      agtacttaa cttgcaaat aacagactt ggttaattg 
      taaaatctc ggctatcgc ctcgtcagg tatttgctt 
      cgcatcagg cgttagtaa ctattgatt gagttttaa 
      tgttaactt ttgcaattt ttttttatt atgtagatt 
      atagtataa atacatctg aaagcataa cagagtata 
      gtgttgttt ctgttgtat tgtcagtat ttaaagaag 
      agcatttag ggtttttga aaaatattt atatgatga 
      aatcatttg ttgtgtcaa aagttttta ataaaaaaa 
      aatatttta cttacatca tttttttgt gtccgactg 
      tacgcagca tgttttatt ctatttcat ttttgtttg 
      acttgaggt ggaataaga ataaaattt acatcggtt 
      acgtaatac ttaaaattt tgtatgaag caaaaattt 
      attagctac acttcgggg atagataca attgacaac 
      atagggaac gtagaaata acagtaaat tcttataag 
      tattattcg ttaatttag aactgagta acatcaaat 
      aaaaatatt tacaacta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY