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Gene information
General information
TC016274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 10252642..10254032
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC016274
    iBeetle screen iB_02565

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016274 RA
      MSCPFGKEA LDKLKQFID VCKSNPDIL SLPDLNFFK 
EFIESFGGK VPPPSKMSS EAPNARAED VASDAESEP 
ESDLELDTE GCVEPDPLD ENQKMGDPN KKVTEEESD 
KSDEKRMEA MGQFSEGNY DKAIELFTE AIELNPSSA 
LLFAKRGQA FLKQTKPNA CIKDCTRAL ELNPDSAAA 
FKFRGRAFS LIGEWEKAA KDLRQACNI DFDEQTDEW 
LKAVTPNAK KIEQHKLKQ ERKRLEKEE REKRERARK 
AREAHAKAS NATRTETNN ANANAAPGD DFYKLLQDP 
EIRAAFTDP EVSAAFADI SSNPANFYK YQSNPKVMA 
LITKLSGKL AGSGFGPGS FPGFPGGFP GFGGMPGGG 
AGAAPPQFQ HDDDNLD
    CDS sequence
    • >TC016274 RA
      atgagctgc ccttttgga aaagaggcc ttggacaag 
ctgaaacaa ttcattgac gtttgcaag tccaaccct 
gacattctg tcgttgcct gatttaaac ttcttcaaa 
gaattcatc gagtccttc ggtggtaaa gtgccccca 
ccttcgaaa atgtcctca gaagccccc aatgcacga 
gctgaagac gtcgcgtcc gatgccgaa tccgaacca 
gaatcagac ttggagctt gatactgaa ggctgtgtg 
gaacctgat cctctggac gaaaaccaa aagatgggc 
gatccaaac aaaaaagtg accgaagaa gagagcgac 
aagtcggac gaaaaacga atggaagcc atgggtcag 
ttctctgaa ggaaactac gacaaagcc atcgaatta 
ttcacagaa gcgatcgaa ttgaatccc tcctctgcg 
ctgctcttc gccaagaga ggacaggcg tttctgaag 
caaactaaa cccaacgct tgcattaaa gattgcacc 
agagcctta gaactgaac cctgactcc gctgcagcc 
ttcaaattc cgcggacgc gctttcagc ctcatcggc 
gagtgggag aaagcggcc aaagacttg cgtcaagcc 
tgcaatatt gactttgac gagcagacc gacgagtgg 
ctcaaagcg gtgacaccg aacgccaaa aagatagag 
caacacaag ttgaaacag gagcggaaa aggctcgag 
aaggaggaa cgggagaag agggaaagg gcccggaaa 
gcgcgagaa gcgcatgcc aaagccagt aatgcgacc 
agaactgaa acaaacaac gccaatgca aacgcagct 
cctggtgat gatttttat aaacttttg caagatccg 
gaaattcgg gctgcgttc accgatcct gaagtctcg 
gcggcattt gctgatatt tcgtcaaat cctgccaac 
ttttataag tatcagtcg aacccgaaa gtcatggct 
ttgattaca aagttgtcg ggaaaattg gcggggtct 
ggttttgga ccggggagc tttccggga ttccctgga 
gggtttccc gggttcggg gggatgcca ggagggggc 
gcgggcgca gccccgccc cagttccaa cacgatgat 
gataacttg gattaa
    mRNA sequence
    • >TC016274 RA
      atttttatt tacattttt gacagtcac gccgcaagc 
cgcatttct agaattttt ccgtgagtg cctatttgg 
atatcgaag tgtgtgttt acgtcccaa aaccaatca 
aaaatgagc tgccctttt ggaaaagag gccttggac 
aagctgaaa caattcatt gacgtttgc aagtccaac 
cctgacatt ctgtcgttg cctgattta aacttcttc 
aaagaattc atcgagtcc ttcggtggt aaagtgccc 
ccaccttcg aaaatgtcc tcagaagcc cccaatgca 
cgagctgaa gacgtcgcg tccgatgcc gaatccgaa 
ccagaatca gacttggag cttgatact gaaggctgt 
gtggaacct gatcctctg gacgaaaac caaaagatg 
ggcgatcca aacaaaaaa gtgaccgaa gaagagagc 
gacaagtcg gacgaaaaa cgaatggaa gccatgggt 
cagttctct gaaggaaac tacgacaaa gccatcgaa 
ttattcaca gaagcgatc gaattgaat ccctcctct 
gcgctgctc ttcgccaag agaggacag gcgtttctg 
aagcaaact aaacccaac gcttgcatt aaagattgc 
accagagcc ttagaactg aaccctgac tccgctgca 
gccttcaaa ttccgcgga cgcgctttc agcctcatc 
ggcgagtgg gagaaagcg gccaaagac ttgcgtcaa 
gcctgcaat attgacttt gacgagcag accgacgag 
tggctcaaa gcggtgaca ccgaacgcc aaaaagata 
gagcaacac aagttgaaa caggagcgg aaaaggctc 
gagaaggag gaacgggag aagagggaa agggcccgg 
aaagcgcga gaagcgcat gccaaagcc agtaatgcg 
accagaact gaaacaaac aacgccaat gcaaacgca 
gctcctggt gatgatttt tataaactt ttgcaagat 
ccggaaatt cgggctgcg ttcaccgat cctgaagtc 
tcggcggca tttgctgat atttcgtca aatcctgcc 
aacttttat aagtatcag tcgaacccg aaagtcatg 
gctttgatt acaaagttg tcgggaaaa ttggcgggg 
tctggtttt ggaccgggg agctttccg ggattccct 
ggagggttt cccgggttc ggggggatg ccaggaggg 
ggcgcgggc gcagccccg ccccagttc caacacgat 
gatgataac ttggattaa acgtttttt tcttacttt 
agttttagt aaaaaaggc tgattttat gtatcttgc 
tacttgtaa attaaaggg ttattaact acttggagt 
tgttttatt tttaacttg atggtggta cacaataat 
tacaataaa agttaaata aattc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY