Status
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Gene information
General information
TC030950
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016254,TC016253
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450232.1: 339331..390200
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030950
    iBeetle screen iB_02563

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 1
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    tropharium slightly shape irregular - Number of animals showing the phenotype: 2

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: there were only two embryos on the slide
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    mesothoracic tracheal opening size increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    urogomphi irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection



    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    Fertility
    egg production: number no/almost no eggs -
    ovary size decreased - Number of animals showing the phenotype: 3

    tropharium size decreased - Number of animals showing the phenotype: 3
    Comment: in 2 of 3 cases the remaining tropharia seemed to be devoid of any germ-line sells
    egg chamber not present - Number of animals showing the phenotype: 3
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030950 RA
      MSFRILQQL RQSYLVYGR VSSRLNSLE VNNYLSQAT 
      TALGISTIV LGVKNADLK TQLEEKTTT ASEVNKTTS 
      IVTTTTTKI PTTTTTTAS EVNKTTSIV TTTTTKIPT 
      TTTTSTTTA TTTTTTTEA PPDMSKYRL PGNAIPTIY 
      DLYLYPDLK TGLFKGKVT AQTDIVATT NEVIMHNNK 
      LNVNQVLIY DQPGTFELD QKYELLTVR KLDGSQFLK 
      GSSVNITVD FDGDMKNRI VGLYKSSYT NPAGEQR
    • >TC030950 RB
      MSFRILQQL RQSYLVYGR VSSRLNSLE VNNYLSQAT 
      TALGISTIV LGVKNADLK TQLEEKTTT ASEVNKTTS 
      IVTTTTTKI PTTTTTTAS EVNKTTSIV TTTTTKIPT 
      TTTTSTTTA TTTTTTTEA PPDMSKYRL PGNAIPTIY 
      DLYLYPDLK TGLFKGKVT AQTDIVATT NEVIMHNNK 
      LNVNQVLIY DQPGTFELD QKYELLTVR KLDGSQFLK 
      GSSVNITVD FDGDMKNRI VGLYKSSYT NPAGEQRNM 
      ATSKFEPTY ARQAFPCFD EPNLKAKYK VHLLKPNDP 
      EYIALSNNP QDSEEIVPE GVMVHFNET VPMSTYLSC 
      FIVSDFKYT NTTFQNGGQ DIPFRVYAS PHQLEKTTY 
      AGEVGKKVI EYYITYFAI PYPLPKLDM VAIPDFVSG 
      AMEHWGLVT YRETALLYN NKTHSASNK QRVAEVVAH 
      ELAHSWFGN LVTMDWWNN LWLNEGFAT YIAAKGIHA 
      ITPEWQMMD QFLINTLHS ILSLDATQG SHPIIQTVE 
      TPDQITEVF DSVSYNKGA SVLRMLETV VTPATFQKG 
      VTNYLKKHE YGNAVTQDL WDEIQAVVG DTLNVTEFM 
      NTVMIADYD PARTQKSSP LNYKWSVPV KFITDLGES 
      DKIYWFNYK SDRLVIDKP ANAKWIKFN PSQIGYYRV 
      NYAENDWKT LTENIESLS IADRTHLLE ESFSIAQSG 
      DLSYEIPLT MTKYLTKET NYIPWGVAS SQLQQIAKY 
      LQNSRLDSG FKNYVVTLL KPAYDNLTW DDSDDSEGH 
      LEKLARVVI LNLACVMDY DEALNEAKS IFGQWIDDN 
      SFEISPNLR SIVYKFGMV TADEVTWNK VFEIFANET 
      DANEKLKLM NGLANVRNP SLLTKLIDL AKDETYVRS 
      QDYFTLLQY ISSNPVGTP IVWDYVREN WPYLVERFT 
      LNDRYLGRL IPAITNRFS TNLKVDEMK SFFAKYPEA 
      GAGAAARQQ ALETVANNI KWLEKYKTV VENWITV
    • >TC030950 RC
      MTSESRSEK PKRKSRKTF AVLLSCCLF LAIVCASLL 
      AALVLIGKH IVYNSAPRN VVKVPKIVP KYVHSRLPR 
      NTFPISYDV VLKPDLETG TFTGTVNIT VNVTAVRND 
      LIVNSKNLN IEAVHLMRD WKSVEIDNV EENVVDEVL 
      IVESEEILY PGIYNLYFK YNGSMLNKM VGLYRSRRI 
      DNNTGLTRN MATSKFEPT YARQAFPCF DEPNLKAKY 
      KVHLLKPND PEYIALSNN PQDSEEIVP EGVMVHFNE 
      TVPMSTYLS CFIVSDFKY TNTTFQNGG QDIPFRVYA 
      SPHQLEKTT YAGEVGKKV IEYYITYFA IPYPLPKLD 
      MVAIPDFVS GAMEHWGLV TYRETALLY NNKTHSASN 
      KQRVAEVVA HELAHSWFG NLVTMDWWN NLWLNEGFA 
      TYIAAKGIH AITPEWQMM DQFLINTLH SILSLDATQ 
      GSHPIIQTV ETPDQITEV FDSVSYNKG ASVLRMLET 
      VVTPATFQK GVTNYLKKH EYGNAVTQD LWDEIQAVV 
      GDTLNVTEF MNTVMIADY DPARTQKSS PLNYKWSVP 
      VKFITDLGE SDKIYWFNY KSDRLVIDK PANAKWIKF 
      NPSQIGYYR VNYAENDWK TLTENIESL SIADRTHLL 
      EESFSIAQS GDLSYEIPL TMTKYLTKE TNYIPWGVA 
      SSQLQQIAK YLQNSRLDS GFKNYVVTL LKPAYDNLT 
      WDDSDDSEG HLEKLARVV ILNLACVMD YDEALNEAK 
      SIFGQWIDD NSFEISPNL RSIVYKFGM VTADEVTWN 
      KVFEIFANE TDANEKLKL MNGLANVRN PSLLTKLID 
      LAKDETYVR SQDYFTLLQ YISSNPVGT PIVWDYVRE 
      NWPYLVERF TLNDRYLGR LIPAITNRF STNLKVDEM 
      KSFFAKYPE AGAGAAARQ QALETVANN IKWLEKYKT 
      VVENWITV
    CDS sequence
    • >TC030950 RA
      atgtctttc agaatatta caacagctc aggcaaagt 
      tacttagtg tatggtcga gtgagctcc agactgaac 
      agtttggaa gttaacaat tacttgagt caagcaaca 
      acagcatta ggaatttcg actatagtt ctcggagtt 
      aaaaatgct gatttaaaa acacaactc gaggaaaaa 
      actacaact gcatcagaa gttaataaa acaacttct 
      atagtgact acaacaact acaaaaata ccaacaaca 
      acaactaca actgcatca gaagttaat aaaacaact 
      tctatagtg actacaaca actacaaaa ataccaaca 
      acaacaaca acatcaaca acaactgct actactacc 
      actactact acagaagct cctccagac atgtccaaa 
      taccgcctg ccaggaaat gccatacct acgatttat 
      gacctctac ctttatccc gacttaaaa acaggactt 
      tttaaagga aaagttacc gctcaaacg gatattgtt 
      gcgacaacg aatgaagtc attatgcat aacaataaa 
      ttaaacgtg aatcaagtt ttgatctat gatcaacca 
      ggaactttt gaactggat cagaaatat gaactacta 
      actgtgaga aaattagac ggatcacaa ttcctaaaa 
      ggtagttct gttaacata acggtcgat tttgacgga 
      gatatgaag aacagaatt gtggggctg tataaaagt 
      tcgtacaca aatccggca ggagaacaa aggtag
    • >TC030950 RB
      atgtctttc agaatatta caacagctc aggcaaagt 
      tacttagtg tatggtcga gtgagctcc agactgaac 
      agtttggaa gttaacaat tacttgagt caagcaaca 
      acagcatta ggaatttcg actatagtt ctcggagtt 
      aaaaatgct gatttaaaa acacaactc gaggaaaaa 
      actacaact gcatcagaa gttaataaa acaacttct 
      atagtgact acaacaact acaaaaata ccaacaaca 
      acaactaca actgcatca gaagttaat aaaacaact 
      tctatagtg actacaaca actacaaaa ataccaaca 
      acaacaaca acatcaaca acaactgct actactacc 
      actactact acagaagct cctccagac atgtccaaa 
      taccgcctg ccaggaaat gccatacct acgatttat 
      gacctctac ctttatccc gacttaaaa acaggactt 
      tttaaagga aaagttacc gctcaaacg gatattgtt 
      gcgacaacg aatgaagtc attatgcat aacaataaa 
      ttaaacgtg aatcaagtt ttgatctat gatcaacca 
      ggaactttt gaactggat cagaaatat gaactacta 
      actgtgaga aaattagac ggatcacaa ttcctaaaa 
      ggtagttct gttaacata acggtcgat tttgacgga 
      gatatgaag aacagaatt gtggggctg tataaaagt 
      tcgtacaca aatccggca ggagaacaa agaaatatg 
      gccacttca aaattcgaa cccacctac gcccgtcaa 
      gctttcccc tgtttcgac gaacccaac ttaaaagcc 
      aaatacaaa gttcatctt ttaaaaccc aatgatcct 
      gaatacatc gctttatca aataatccg caagatagt 
      gaagaaatc gtccctgaa ggtgttatg gtacatttt 
      aacgaaacg gtaccaatg tccacgtat ctatcatgt 
      tttatcgtg tcagatttt aaatataca aatacaacg 
      tttcaaaat ggtggacaa gacattcct ttcagggtt 
      tacgcaagt ccccatcaa ctcgaaaag acgacttat 
      gcgggagaa gttgggaaa aaagttata gagtactat 
      ataacgtac tttgctatt ccatatccg ctaccaaaa 
      ttggacatg gtagcgatt cccgatttc gtttcggga 
      gcaatggaa cactggggt ctcgtgacc tatcgagaa 
      accgctctt ttatacaac aataaaaca cattcggcc 
      agtaacaaa caaagagta gcggaagta gtagctcac 
      gaacttgca catagctgg tttggaaat ctagtcacg 
      atggattgg tggaacaat ttatggcta aatgaaggt 
      tttgcaacg tatattgcc gctaaagga atccatgca 
      atcacaccg gaatggcaa atgatggac caattcctc 
      attaacaca cttcattca attctttca ttggacgcc 
      acacaaggt tcacaccca atcattcaa actgttgaa 
      acgcctgat caaatcacc gaagtgttt gattccgtc 
      tcgtacaat aaaggcgcg tctgtattg aggatgtta 
      gagacggtt gtaactcca gcaacattt caaaaaggt 
      gtaactaat tatctaaaa aaacatgag tatggtaat 
      gcagttaca caggacttg tgggatgaa atacaagct 
      gtggtgggt gatacttta aatgttacc gaatttatg 
      aatactgtg atgatcgcg gattatgac ccggcaagg 
      acgcagaaa agctcacct ttaaattac aaatggagt 
      gtccccgtc aaattcatt actgatttg ggagaatcg 
      gataaaatc tactggttt aattacaaa tctgatcgg 
      ttggtcatt gataaaccg gccaatgcc aaatggatt 
      aaatttaac ccaagtcag atcggttat taccgcgtt 
      aattatgct gaaaatgat tggaaaaca cttactgaa 
      aatattgag tcgttatca attgccgac cgaacacat 
      ttacttgaa gaaagtttc agtatcgca cagtcgggc 
      gatttgagc tacgaaatt cccctaact atgactaaa 
      tatctaact aaggaaact aactatatt ccttgggga 
      gttgcctct tcacagttg caacaaatc gctaaatat 
      ttgcaaaat tcgagactg gattcaggg tttaagaat 
      tatgtggtc actttattg aagccggct tatgacaat 
      ttgacttgg gacgacagc gatgacagt gaaggccat 
      ttggagaag ttggcacgt gtggttatt ttaaatcta 
      gcctgtgtt atggactac gatgaggct ttgaatgag 
      gctaaaagc atttttggg caatggatc gatgataat 
      tcgtttgag atttcgccg aatttgcga tcgattgtt 
      tataaattt ggtatggta actgctgat gaagttact 
      tggaataaa gttttcgaa atttttgct aatgagact 
      gatgccaat gaaaaacta aaattgatg aatggttta 
      gccaatgtg cgaaatcca tcacttttg accaaattg 
      attgattta gccaaagat gagacttat gttcgaagt 
      caagattat ttcactttg ttgcaatat atttcatct 
      aatcctgtt ggaactcca attgtttgg gattatgtc 
      cgtgaaaat tggccctat ttagttgaa aggtttacc 
      ttaaacgac cgttatttg ggacgactt attccagcg 
      ataactaac cgattttca acaaatttg aaagtcgat 
      gagatgaaa tctttcttt gctaagtat ccagaggcc 
      ggtgcaggt gctgccgca cgacaacaa gctttagaa 
      actgtcgct aataatatc aagtggttg gagaaatat 
      aaaacggtt gtcgaaaac tggatcact gtttaa
    • >TC030950 RC
      atgacttcg gaatcgcgc agtgaaaaa ccgaaacga 
      aaatcgcgg aaaaccttc gcagtgttg ctcagttgt 
      tgtcttttc cttgcgatt gtgtgtgct tcacttttg 
      gcggccctt gtccttatc ggaaaacac attgtgtat 
      aactccgct ccgcgaaat gtggttaaa gtgcccaaa 
      atagtgccg aaatatgtt cattcacga ttaccacga 
      aacactttt ccgattagt tacgatgtt gtgcttaag 
      ccggacctg gaaacggga actttcacg ggaacggta 
      aacattacg gtaaacgtg acggcagtc cggaacgac 
      ttgatcgta aacagcaaa aatttgaat attgaagcg 
      gtgcatttg atgcgagat tggaaaagt gttgagatc 
      gataatgtg gaggaaaat gtcgttgat gaggttttg 
      attgttgaa agtgaagag attctctat ccggggatt 
      tataattta tatttcaag tacaacgga agtatgttg 
      aacaaaatg gtcggtttg tataggagt agacgaata 
      gacaacaac accggcttg acgagaaat atggccact 
      tcaaaattc gaacccacc tacgcccgt caagctttc 
      ccctgtttc gacgaaccc aacttaaaa gccaaatac 
      aaagttcat cttttaaaa cccaatgat cctgaatac 
      atcgcttta tcaaataat ccgcaagat agtgaagaa 
      atcgtccct gaaggtgtt atggtacat tttaacgaa 
      acggtacca atgtccacg tatctatca tgttttatc 
      gtgtcagat tttaaatat acaaataca acgtttcaa 
      aatggtgga caagacatt cctttcagg gtttacgca 
      agtccccat caactcgaa aagacgact tatgcggga 
      gaagttggg aaaaaagtt atagagtac tatataacg 
      tactttgct attccatat ccgctacca aaattggac 
      atggtagcg attcccgat ttcgtttcg ggagcaatg 
      gaacactgg ggtctcgtg acctatcga gaaaccgct 
      cttttatac aacaataaa acacattcg gccagtaac 
      aaacaaaga gtagcggaa gtagtagct cacgaactt 
      gcacatagc tggtttgga aatctagtc acgatggat 
      tggtggaac aatttatgg ctaaatgaa ggttttgca 
      acgtatatt gccgctaaa ggaatccat gcaatcaca 
      ccggaatgg caaatgatg gaccaattc ctcattaac 
      acacttcat tcaattctt tcattggac gccacacaa 
      ggttcacac ccaatcatt caaactgtt gaaacgcct 
      gatcaaatc accgaagtg tttgattcc gtctcgtac 
      aataaaggc gcgtctgta ttgaggatg ttagagacg 
      gttgtaact ccagcaaca tttcaaaaa ggtgtaact 
      aattatcta aaaaaacat gagtatggt aatgcagtt 
      acacaggac ttgtgggat gaaatacaa gctgtggtg 
      ggtgatact ttaaatgtt accgaattt atgaatact 
      gtgatgatc gcggattat gacccggca aggacgcag 
      aaaagctca cctttaaat tacaaatgg agtgtcccc 
      gtcaaattc attactgat ttgggagaa tcggataaa 
      atctactgg tttaattac aaatctgat cggttggtc 
      attgataaa ccggccaat gccaaatgg attaaattt 
      aacccaagt cagatcggt tattaccgc gttaattat 
      gctgaaaat gattggaaa acacttact gaaaatatt 
      gagtcgtta tcaattgcc gaccgaaca catttactt 
      gaagaaagt ttcagtatc gcacagtcg ggcgatttg 
      agctacgaa attccccta actatgact aaatatcta 
      actaaggaa actaactat attccttgg ggagttgcc 
      tcttcacag ttgcaacaa atcgctaaa tatttgcaa 
      aattcgaga ctggattca gggtttaag aattatgtg 
      gtcacttta ttgaagccg gcttatgac aatttgact 
      tgggacgac agcgatgac agtgaaggc catttggag 
      aagttggca cgtgtggtt attttaaat ctagcctgt 
      gttatggac tacgatgag gctttgaat gaggctaaa 
      agcattttt gggcaatgg atcgatgat aattcgttt 
      gagatttcg ccgaatttg cgatcgatt gtttataaa 
      tttggtatg gtaactgct gatgaagtt acttggaat 
      aaagttttc gaaattttt gctaatgag actgatgcc 
      aatgaaaaa ctaaaattg atgaatggt ttagccaat 
      gtgcgaaat ccatcactt ttgaccaaa ttgattgat 
      ttagccaaa gatgagact tatgttcga agtcaagat 
      tatttcact ttgttgcaa tatatttca tctaatcct 
      gttggaact ccaattgtt tgggattat gtccgtgaa 
      aattggccc tatttagtt gaaaggttt accttaaac 
      gaccgttat ttgggacga cttattcca gcgataact 
      aaccgattt tcaacaaat ttgaaagtc gatgagatg 
      aaatctttc tttgctaag tatccagag gccggtgca 
      ggtgctgcc gcacgacaa caagcttta gaaactgtc 
      gctaataat atcaagtgg ttggagaaa tataaaacg 
      gttgtcgaa aactggatc actgtttaa
    mRNA sequence
    • >TC030950 RA
      atgattgtg tatgtgaaa aagtgaggt tatagttta 
      ttgtttttg tctgcaaaa tacacctaa attgagtta 
      aaatgaatg atacgaaag tgtaacagt aagaattat 
      gtctttcag aatattaca acagctcag gcaaagtta 
      cttagtgta tggtcgagt gagctccag actgaacag 
      tttggaagt taacaatta cttgagtca agcaacaac 
      agcattagg aatttcgac tatagttct cggagttaa 
      aaatgctga tttaaaaac acaactcga ggaaaaaac 
      tacaactgc atcagaagt taataaaac aacttctat 
      agtgactac aacaactac aaaaatacc aacaacaac 
      aactacaac tgcatcaga agttaataa aacaacttc 
      tatagtgac tacaacaac tacaaaaat accaacaac 
      aacaacaac atcaacaac aactgctac tactaccac 
      tactactac agaagctcc tccagacat gtccaaata 
      ccgcctgcc aggaaatgc catacctac gatttatga 
      cctctacct ttatcccga cttaaaaac aggactttt 
      taaaggaaa agttaccgc tcaaacgga tattgttgc 
      gacaacgaa tgaagtcat tatgcataa caataaatt 
      aaacgtgaa tcaagtttt gatctatga tcaaccagg 
      aacttttga actggatca gaaatatga actactaac 
      tgtgagaaa attagacgg atcacaatt cctaaaagg 
      tagttctgt taacataac ggtcgattt tgacggaga 
      tatgaagaa cagaattgt ggggctgta taaaagttc 
      gtacacaaa tccggcagg agaacaaag gtaggactt 
      ttttatgta atagtggtt ttaaacacg aacgagcga 
      tgcgagagt gacagaatt tt
    • >TC030950 RB
      atgattgtg tatgtgaaa aagtgaggt tatagttta 
      ttgtttttg tctgcaaaa tacacctaa attgagtta 
      aaatgaatg atacgaaag tgtaacagt aagaattat 
      gtctttcag aatattaca acagctcag gcaaagtta 
      cttagtgta tggtcgagt gagctccag actgaacag 
      tttggaagt taacaatta cttgagtca agcaacaac 
      agcattagg aatttcgac tatagttct cggagttaa 
      aaatgctga tttaaaaac acaactcga ggaaaaaac 
      tacaactgc atcagaagt taataaaac aacttctat 
      agtgactac aacaactac aaaaatacc aacaacaac 
      aactacaac tgcatcaga agttaataa aacaacttc 
      tatagtgac tacaacaac tacaaaaat accaacaac 
      aacaacaac atcaacaac aactgctac tactaccac 
      tactactac agaagctcc tccagacat gtccaaata 
      ccgcctgcc aggaaatgc catacctac gatttatga 
      cctctacct ttatcccga cttaaaaac aggactttt 
      taaaggaaa agttaccgc tcaaacgga tattgttgc 
      gacaacgaa tgaagtcat tatgcataa caataaatt 
      aaacgtgaa tcaagtttt gatctatga tcaaccagg 
      aacttttga actggatca gaaatatga actactaac 
      tgtgagaaa attagacgg atcacaatt cctaaaagg 
      tagttctgt taacataac ggtcgattt tgacggaga 
      tatgaagaa cagaattgt ggggctgta taaaagttc 
      gtacacaaa tccggcagg agaacaaag aaatatggc 
      cacttcaaa attcgaacc cacctacgc ccgtcaagc 
      tttcccctg tttcgacga acccaactt aaaagccaa 
      atacaaagt tcatctttt aaaacccaa tgatcctga 
      atacatcgc tttatcaaa taatccgca agatagtga 
      agaaatcgt ccctgaagg tgttatggt acattttaa 
      cgaaacggt accaatgtc cacgtatct atcatgttt 
      tatcgtgtc agattttaa atatacaaa tacaacgtt 
      tcaaaatgg tggacaaga cattccttt cagggttta 
      cgcaagtcc ccatcaact cgaaaagac gacttatgc 
      gggagaagt tgggaaaaa agttataga gtactatat 
      aacgtactt tgctattcc atatccgct accaaaatt 
      ggacatggt agcgattcc cgatttcgt ttcgggagc 
      aatggaaca ctggggtct cgtgaccta tcgagaaac 
      cgctctttt atacaacaa taaaacaca ttcggccag 
      taacaaaca aagagtagc ggaagtagt agctcacga 
      acttgcaca tagctggtt tggaaatct agtcacgat 
      ggattggtg gaacaattt atggctaaa tgaaggttt 
      tgcaacgta tattgccgc taaaggaat ccatgcaat 
      cacaccgga atggcaaat gatggacca attcctcat 
      taacacact tcattcaat tctttcatt ggacgccac 
      acaaggttc acacccaat cattcaaac tgttgaaac 
      gcctgatca aatcaccga agtgtttga ttccgtctc 
      gtacaataa aggcgcgtc tgtattgag gatgttaga 
      gacggttgt aactccagc aacatttca aaaaggtgt 
      aactaatta tctaaaaaa acatgagta tggtaatgc 
      agttacaca ggacttgtg ggatgaaat acaagctgt 
      ggtgggtga tactttaaa tgttaccga atttatgaa 
      tactgtgat gatcgcgga ttatgaccc ggcaaggac 
      gcagaaaag ctcaccttt aaattacaa atggagtgt 
      ccccgtcaa attcattac tgatttggg agaatcgga 
      taaaatcta ctggtttaa ttacaaatc tgatcggtt 
      ggtcattga taaaccggc caatgccaa atggattaa 
      atttaaccc aagtcagat cggttatta ccgcgttaa 
      ttatgctga aaatgattg gaaaacact tactgaaaa 
      tattgagtc gttatcaat tgccgaccg aacacattt 
      acttgaaga aagtttcag tatcgcaca gtcgggcga 
      tttgagcta cgaaattcc cctaactat gactaaata 
      tctaactaa ggaaactaa ctatattcc ttggggagt 
      tgcctcttc acagttgca acaaatcgc taaatattt 
      gcaaaattc gagactgga ttcagggtt taagaatta 
      tgtggtcac tttattgaa gccggctta tgacaattt 
      gacttggga cgacagcga tgacagtga aggccattt 
      ggagaagtt ggcacgtgt ggttatttt aaatctagc 
      ctgtgttat ggactacga tgaggcttt gaatgaggc 
      taaaagcat ttttgggca atggatcga tgataattc 
      gtttgagat ttcgccgaa tttgcgatc gattgttta 
      taaatttgg tatggtaac tgctgatga agttacttg 
      gaataaagt tttcgaaat ttttgctaa tgagactga 
      tgccaatga aaaactaaa attgatgaa tggtttagc 
      caatgtgcg aaatccatc acttttgac caaattgat 
      tgatttagc caaagatga gacttatgt tcgaagtca 
      agattattt cactttgtt gcaatatat ttcatctaa 
      tcctgttgg aactccaat tgtttggga ttatgtccg 
      tgaaaattg gccctattt agttgaaag gtttacctt 
      aaacgaccg ttatttggg acgacttat tccagcgat 
      aactaaccg attttcaac aaatttgaa agtcgatga 
      gatgaaatc tttctttgc taagtatcc agaggccgg 
      tgcaggtgc tgccgcacg acaacaagc tttagaaac 
      tgtcgctaa taatatcaa gtggttgga gaaatataa 
      aacggttgt cgaaaactg gatcactgt ttaatgtgc 
      tacaaggaa cctgttgta aattgctca aactgtata 
      ttttttaaa aattgtgaa aaatatacc tacttaaaa 
      aaa
    • >TC030950 RC
      accagcact ggagagtaa cgcactcga aacaggtct 
      cttctaacc acgtgaaat gtgccaatg aacgatgac 
      ttcggaatc gcgcagtga aaaaccgaa acgaaaatc 
      gcggaaaac cttcgcagt gttgctcag ttgttgtct 
      tttccttgc gattgtgtg tgcttcact tttggcggc 
      ccttgtcct tatcggaaa acacattgt gtataactc 
      cgctccgcg aaatgtggt taaagtgcc caaaatagt 
      gccgaaata tgttcattc acgattacc acgaaacac 
      ttttccgat tagttacga tgttgtgct taagccgga 
      cctggaaac gggaacttt cacgggaac ggtaaacat 
      tacggtaaa cgtgacggc agtccggaa cgacttgat 
      cgtaaacag caaaaattt gaatattga agcggtgca 
      tttgatgcg agattggaa aagtgttga gatcgataa 
      tgtggagga aaatgtcgt tgatgaggt tttgattgt 
      tgaaagtga agagattct ctatccggg gatttataa 
      tttatattt caagtacaa cggaagtat gttgaacaa 
      aatggtcgg tttgtatag gagtagacg aatagacaa 
      caacaccgg cttgacgag aaatatggc cacttcaaa 
      attcgaacc cacctacgc ccgtcaagc tttcccctg 
      tttcgacga acccaactt aaaagccaa atacaaagt 
      tcatctttt aaaacccaa tgatcctga atacatcgc 
      tttatcaaa taatccgca agatagtga agaaatcgt 
      ccctgaagg tgttatggt acattttaa cgaaacggt 
      accaatgtc cacgtatct atcatgttt tatcgtgtc 
      agattttaa atatacaaa tacaacgtt tcaaaatgg 
      tggacaaga cattccttt cagggttta cgcaagtcc 
      ccatcaact cgaaaagac gacttatgc gggagaagt 
      tgggaaaaa agttataga gtactatat aacgtactt 
      tgctattcc atatccgct accaaaatt ggacatggt 
      agcgattcc cgatttcgt ttcgggagc aatggaaca 
      ctggggtct cgtgaccta tcgagaaac cgctctttt 
      atacaacaa taaaacaca ttcggccag taacaaaca 
      aagagtagc ggaagtagt agctcacga acttgcaca 
      tagctggtt tggaaatct agtcacgat ggattggtg 
      gaacaattt atggctaaa tgaaggttt tgcaacgta 
      tattgccgc taaaggaat ccatgcaat cacaccgga 
      atggcaaat gatggacca attcctcat taacacact 
      tcattcaat tctttcatt ggacgccac acaaggttc 
      acacccaat cattcaaac tgttgaaac gcctgatca 
      aatcaccga agtgtttga ttccgtctc gtacaataa 
      aggcgcgtc tgtattgag gatgttaga gacggttgt 
      aactccagc aacatttca aaaaggtgt aactaatta 
      tctaaaaaa acatgagta tggtaatgc agttacaca 
      ggacttgtg ggatgaaat acaagctgt ggtgggtga 
      tactttaaa tgttaccga atttatgaa tactgtgat 
      gatcgcgga ttatgaccc ggcaaggac gcagaaaag 
      ctcaccttt aaattacaa atggagtgt ccccgtcaa 
      attcattac tgatttggg agaatcgga taaaatcta 
      ctggtttaa ttacaaatc tgatcggtt ggtcattga 
      taaaccggc caatgccaa atggattaa atttaaccc 
      aagtcagat cggttatta ccgcgttaa ttatgctga 
      aaatgattg gaaaacact tactgaaaa tattgagtc 
      gttatcaat tgccgaccg aacacattt acttgaaga 
      aagtttcag tatcgcaca gtcgggcga tttgagcta 
      cgaaattcc cctaactat gactaaata tctaactaa 
      ggaaactaa ctatattcc ttggggagt tgcctcttc 
      acagttgca acaaatcgc taaatattt gcaaaattc 
      gagactgga ttcagggtt taagaatta tgtggtcac 
      tttattgaa gccggctta tgacaattt gacttggga 
      cgacagcga tgacagtga aggccattt ggagaagtt 
      ggcacgtgt ggttatttt aaatctagc ctgtgttat 
      ggactacga tgaggcttt gaatgaggc taaaagcat 
      ttttgggca atggatcga tgataattc gtttgagat 
      ttcgccgaa tttgcgatc gattgttta taaatttgg 
      tatggtaac tgctgatga agttacttg gaataaagt 
      tttcgaaat ttttgctaa tgagactga tgccaatga 
      aaaactaaa attgatgaa tggtttagc caatgtgcg 
      aaatccatc acttttgac caaattgat tgatttagc 
      caaagatga gacttatgt tcgaagtca agattattt 
      cactttgtt gcaatatat ttcatctaa tcctgttgg 
      aactccaat tgtttggga ttatgtccg tgaaaattg 
      gccctattt agttgaaag gtttacctt aaacgaccg 
      ttatttggg acgacttat tccagcgat aactaaccg 
      attttcaac aaatttgaa agtcgatga gatgaaatc 
      tttctttgc taagtatcc agaggccgg tgcaggtgc 
      tgccgcacg acaacaagc tttagaaac tgtcgctaa 
      taatatcaa gtggttgga gaaatataa aacggttgt 
      cgaaaactg gatcactgt ttaatgtgc tacaaggaa 
      cctgttgta aattgctca aactgtata ttttttaaa 
      aattgtgaa aaatatacc tacttaaaa aaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY