Status
Please wait ...
Gene information
General information
TC016226 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 191310..196345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016226
    iBeetle screen iB_02561
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 80.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection



    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    iBeetle screen iB_07142
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016226 RA
      MEGEQFSLC WNNFHSNLS SGFNSLLKD EDLVDVTLA 
      AGGRFMKAH KTVLSVCSP FFKELFRAN PSKHPIVIL 
      PDVNYKALC NLLQFMYQG EVSVSQEEI PMFMRVAEM 
      LKVKGLTDN SSSSSSSSE SNGYTASSR IPYSFSDQR 
      QIMKRPRPV KKVIKPMQP LRPIDSPKP RSPHPPPPK 
      RPHIEEPPI RQEEQEFPE ALVKPKEEP LDDTESNTS 
      QKFEENSLD LSTMLDTKI GESSSWQTS EIINKPQDS 
      STPGTLQCI LGRRGNPRL IVDGYVFYK KSVYKGKAF 
      WYCKNSRSP EKCQAVCWT MNGNIVKWP YMHTHPVIP 
      DVFNPDEDT EVPIQNLQE VLWQTSYS
    CDS sequence
    • >TC016226 RA
      atggaaggc gagcagttt tctttgtgt tggaacaat 
      tttcacagt aatttaagt tcggggttc aattccctg 
      ctcaaggat gaggatttg gtggacgtg actttggca 
      gcagggggg cgcttcatg aaggcccac aagactgtc 
      ctatcggtg tgtagcccc tttttcaag gagctgttc 
      agggccaac cctagcaag caccccatt gtcatcctg 
      cctgatgta aattacaag gctctgtgt aatttactg 
      cagtttatg taccaaggg gaggtcagt gtgagtcaa 
      gaggaaata cccatgttt atgagggtg gcggagatg 
      ctgaaagtc aaggggttg actgataac agctcgtct 
      tcgtcctcg tcctccgaa tcaaacggc tacactgca 
      tccagtcga atcccgtac agtttttcc gaccaaaga 
      caaatcatg aaacgacca agacctgtc aaaaaagtg 
      ataaagccc atgcagccc ctgcgacct attgacagc 
      cccaaacca aggtcacct catcctccg ccccctaaa 
      cggccccac attgaggag ccccctatc agacaggaa 
      gaacaagag tttcctgaa gcgttagtg aagccaaag 
      gaagagcct ctggatgat accgaaagt aacacttca 
      caaaagttt gaagaaaat agtttagat ttgtcaacg 
      atgttggat acgaaaatt ggggagtcg tcaagttgg 
      caaacctcc gaaattatc aataaacct caggattcg 
      tcaacacca ggcaccctg cagtgcatt ctgggaaga 
      cgcggtaat ccccgtctg atcgttgat ggttacgtc 
      ttttataag aagagtgtg tacaaaggc aaggcgttc 
      tggtattgt aaaaacagc cgatcgccg gaaaaatgc 
      caagcggtt tgttggaca atgaacgga aacattgtc 
      aaatggccg tacatgcac acgcatccg gtcattccc 
      gacgtgttt aacccggac gaagacact gaagttcct 
      atacaaaat ttacaagaa gtattatgg cagacctcg 
      tatagttaa
    mRNA sequence
    • >TC016226 RA
      caagtcgta aataattat tctgcccct tttttgttg 
      ataagacat tgacataag aaaaatgac gtcacactt 
      gtcttgtcc tccattttc atgatgctt gtatgatgc 
      ttgaaaaaa atcggttta aattgcaaa taagtgtga 
      atttgctac aagtgtgag taaaaacac ttactttcg 
      cccacactt atgattatt caacaggtg taaaaatag 
      ccggttaaa atggaaggc gagcagttt tctttgtgt 
      tggaacaat tttcacagt aatttaagt tcggggttc 
      aattccctg ctcaaggat gaggatttg gtggacgtg 
      actttggca gcagggggg cgcttcatg aaggcccac 
      aagactgtc ctatcggtg tgtagcccc tttttcaag 
      gagctgttc agggccaac cctagcaag caccccatt 
      gtcatcctg cctgatgta aattacaag gctctgtgt 
      aatttactg cagtttatg taccaaggg gaggtcagt 
      gtgagtcaa gaggaaata cccatgttt atgagggtg 
      gcggagatg ctgaaagtc aaggggttg actgataac 
      agctcgtct tcgtcctcg tcctccgaa tcaaacggc 
      tacactgca tccagtcga atcccgtac agtttttcc 
      gaccaaaga caaatcatg aaacgacca agacctgtc 
      aaaaaagtg ataaagccc atgcagccc ctgcgacct 
      attgacagc cccaaacca aggtcacct catcctccg 
      ccccctaaa cggccccac attgaggag ccccctatc 
      agacaggaa gaacaagag tttcctgaa gcgttagtg 
      aagccaaag gaagagcct ctggatgat accgaaagt 
      aacacttca caaaagttt gaagaaaat agtttagat 
      ttgtcaacg atgttggat acgaaaatt ggggagtcg 
      tcaagttgg caaacctcc gaaattatc aataaacct 
      caggattcg tcaacacca ggcaccctg cagtgcatt 
      ctgggaaga cgcggtaat ccccgtctg atcgttgat 
      ggttacgtc ttttataag aagagtgtg tacaaaggc 
      aaggcgttc tggtattgt aaaaacagc cgatcgccg 
      gaaaaatgc caagcggtt tgttggaca atgaacgga 
      aacattgtc aaatggccg tacatgcac acgcatccg 
      gtcattccc gacgtgttt aacccggac gaagacact 
      gaagttcct atacaaaat ttacaagaa gtattatgg 
      cagacctcg tatagttaa gaagatttt ttttgtgat 
      attttttgt gagtgtcaa attttagtg gtagaaaaa 
      attcgatat gtagccaaa acatccagt gacgtttgt 
      gagactgtt ttgtatttc tctcaattg taagttctt 
      tttttaaat aaagaagtt tctaatcaa caggtggcg 
      gtttgatgt tgattaaaa gtgggcgtg gccagttgg 
      ttctaatcc accaaggtc atatttaca tacattgtg 
      atacgaaaa ataaccgac atatgtggg tgtgtgtga 
      agaagaaaa cgctcaatt gtgaaggct gtctcacga 
      ccttgaacg gacccatac ttctctcgt acagtgaac 
      acaaccatc cacaattac aggacgtta tacaaagac 
      tgcaagtac ctagctgta gtgtgtcac ccgaactga 
      tgtaacaca ccaatgcca tagttttcg gacaaagaa 
      ttgttattt atttttgtt gatttatac caaatattt 
      atttccgtt attttattt tctatgacg agttgggag 
      tgattttat tgtttttgt tgaccagtc aggatattt 
      tatttattt atttattat cgaacgttt ttgtattaa 
      ttttccgtt aataaaaaa tgggaaccc ac