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Gene information
General information
TC016224 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 138819..141218
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016224
    iBeetle screen iB_02560
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016224 RA
      MLSTRARLL LSLLNKSDV FRSYCKKAT KGKFEFPKR 
      TIFKSYGQP THETHPQLL QKDEITPLI KRSEFQDRR 
      QKLMEQVCV YVNERNPSM REHLIVIPS ATKQYMSDK 
      IPYFFRQNT DFLYLTGCL EPDSCLILA TTGAPSQHC 
      STLFLREKD DHSELWDGP RTGPDNAPN VFGVDQSLP 
      MSEMENYLQ SFFKSNNKF SLWYDFMNP VQIDVHKTM 
      VDYLNYMRQ KMWESPKVF IHKQRLIKS PAEVALMQQ 
      SCDIASRAI IETIKASHP GINESQIFA TVDYECRMQ 
      GAEYLAYPP VVAGGNRAT TIHYINNNQ VVQDGEMVL 
      MDAGCEFHG YSSDITRTW PINGKFSTS QREVYEVVL 
      DVQKKLIQL CENFPTLDS LFDSMCVLL GKGLQEIGL 
      IPKILTNQA LTRAAYQFC PHHVSHYLG MDVHDTPLI 
      TRNVKIQPG MIVTVEPGV YINHKHQQL PKEFLGMGV 
      RIEDDVLIT ESGPVILSR NCPKEVSDI EDISSRNV
    CDS sequence
    • >TC016224 RA
      atgttgtcg acacgagcc cggctcctt ttatcactt 
      ttgaacaaa tcagatgtc ttccgttcc tattgcaaa 
      aaagccaca aagggcaag ttcgagttc cccaagcgg 
      acgattttc aagtcctac ggccaaccg acacacgag 
      acccacccc cagctcttg caaaaagac gaaatcacc 
      cccttgata aaacgctcc gagttccag gaccggaga 
      cagaaacta atggagcaa gtgtgtgtc tacgtcaat 
      gagcggaac ccctccatg cgggaacac ttgatagtt 
      attccatca gccaccaaa cagtacatg tccgataaa 
      atcccgtat ttttttcgc caaaacacc gattttctc 
      tacttgact ggctgtttg gagcctgat agttgtctc 
      atcttggcc acgacgggg gcccccagc cagcactgt 
      tccaccctg tttttgcgc gagaaggac gaccattcc 
      gagctgtgg gacggcccc aggacgggg cccgacaac 
      gcccccaac gtgttcggg gtcgaccag agtttgcca 
      atgagcgag atggagaat tacttgcag tcgtttttc 
      aaatcgaac aataaattc agtctttgg tatgacttc 
      atgaacccc gtccaaatc gacgtgcac aaaacaatg 
      gtcgattat ttgaactac atgcggcaa aaaatgtgg 
      gaatccccg aaagttttt atacataag caaaggctg 
      attaaaagt ccagccgag gtagcgctg atgcaacaa 
      tcgtgtgat attgcctca agggcgatt attgaaact 
      atcaaggct tcacatccc gggataaat gagagtcag 
      atttttgca acagttgat tatgagtgt agaatgcaa 
      ggggccgaa tatttggca tacccccct gtggtagca 
      gggggtaat agggccaca actatacat tatattaat 
      aataatcaa gtagtccag gacggggag atggtttta 
      atggacgcg ggctgcgaa tttcacggt tattcaagc 
      gacattacc agaacctgg cccatcaat ggtaaattc 
      agcaccagt cagagagaa gtgtacgaa gttgttcta 
      gacgttcaa aaaaaattg attcaattg tgtgaaaat 
      tttcccact ttggacagt ttattcgac tcaatgtgt 
      gtcttacta gggaaaggt ttgcaagaa attggctta 
      attccgaaa atattgacc aatcaagcc ttgacacgc 
      gccgcctat cagttctgt ccgcaccac gtgtcgcat 
      tatttggga atggatgtt cacgatacg cctttaatt 
      actcgaaat gttaaaata caacctggg atgattgtt 
      acggttgaa cccggggtt tatattaat cacaaacac 
      caacaatta ccgaaagaa tttctcgga atgggtgtc 
      cgaattgaa gacgatgtt ttaatcact gagtccggt 
      cctgtcatt ctcagtcgt aactgtccg aaagaagtg 
      tcagatatt gaagatata tctagtcgg aatgtgtga
    mRNA sequence
    • >TC016224 RA
      tacgtttcc taacctcta aaaaaaaat ttttagttt 
      ttaagcctt cagaaacat caaatctgt gatattgca 
      atgttgtcg acacgagcc cggctcctt ttatcactt 
      ttgaacaaa tcagatgtc ttccgttcc tattgcaaa 
      aaagccaca aagggcaag ttcgagttc cccaagcgg 
      acgattttc aagtcctac ggccaaccg acacacgag 
      acccacccc cagctcttg caaaaagac gaaatcacc 
      cccttgata aaacgctcc gagttccag gaccggaga 
      cagaaacta atggagcaa gtgtgtgtc tacgtcaat 
      gagcggaac ccctccatg cgggaacac ttgatagtt 
      attccatca gccaccaaa cagtacatg tccgataaa 
      atcccgtat ttttttcgc caaaacacc gattttctc 
      tacttgact ggctgtttg gagcctgat agttgtctc 
      atcttggcc acgacgggg gcccccagc cagcactgt 
      tccaccctg tttttgcgc gagaaggac gaccattcc 
      gagctgtgg gacggcccc aggacgggg cccgacaac 
      gcccccaac gtgttcggg gtcgaccag agtttgcca 
      atgagcgag atggagaat tacttgcag tcgtttttc 
      aaatcgaac aataaattc agtctttgg tatgacttc 
      atgaacccc gtccaaatc gacgtgcac aaaacaatg 
      gtcgattat ttgaactac atgcggcaa aaaatgtgg 
      gaatccccg aaagttttt atacataag caaaggctg 
      attaaaagt ccagccgag gtagcgctg atgcaacaa 
      tcgtgtgat attgcctca agggcgatt attgaaact 
      atcaaggct tcacatccc gggataaat gagagtcag 
      atttttgca acagttgat tatgagtgt agaatgcaa 
      ggggccgaa tatttggca tacccccct gtggtagca 
      gggggtaat agggccaca actatacat tatattaat 
      aataatcaa gtagtccag gacggggag atggtttta 
      atggacgcg ggctgcgaa tttcacggt tattcaagc 
      gacattacc agaacctgg cccatcaat ggtaaattc 
      agcaccagt cagagagaa gtgtacgaa gttgttcta 
      gacgttcaa aaaaaattg attcaattg tgtgaaaat 
      tttcccact ttggacagt ttattcgac tcaatgtgt 
      gtcttacta gggaaaggt ttgcaagaa attggctta 
      attccgaaa atattgacc aatcaagcc ttgacacgc 
      gccgcctat cagttctgt ccgcaccac gtgtcgcat 
      tatttggga atggatgtt cacgatacg cctttaatt 
      actcgaaat gttaaaata caacctggg atgattgtt 
      acggttgaa cccggggtt tatattaat cacaaacac 
      caacaatta ccgaaagaa tttctcgga atgggtgtc 
      cgaattgaa gacgatgtt ttaatcact gagtccggt 
      cctgtcatt ctcagtcgt aactgtccg aaagaagtg 
      tcagatatt gaagatata tctagtcgg aatgtgtga 
      tgtcaattg ttatttaat ttaacaatt taaaaataa 
      aaaatattt tattcaatt cgagcgttg agctacaat 
      ctctacaat atatgtata attcggtta aaaggaatg 
      ttttatcac ttgtacata aataagtgc cccagagac 
      gcactctgt catttgttc gcccagttt tcggtgcgt 
      gtcgcttta ccaatccaa gtctttgtg gggttcccc 
      gcccggata ctcgccggt aaggcaggc agtgcagtg 
      accgacctt gtgcgaatt tttggtttt gatgtcgga 
      gcggacggc tttcaccaa accttccac actcagata 
      tttcaaact gttggcacc tgttgaaga aatagtcat 
      ttaaata