Status
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Gene information
General information
TC031427
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016218
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4336797..4345933
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031427
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02558

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031427 RA
      MLRLVILAT CLTLGHFAD HDTFKNCSR VEFCNTLRN 
      RQPFDKYAV DPSTITIDD NGSVKMTLK AKKGSDLQL 
      ELLALVDRT FRLRIKETA STRYELHDV LVAEPQLAH 
      IDGVLQQDN YVTIQVGET TAQIFYDPF VVNFYLVQV 
      HQVVIDGST LTFDESLDQ AFAFNVKFP AALQLYGLH 
      EHADSLALR DTNGSDPYR LRNLDVAGY ELDSPMALY 
      GAVPVLYGW GTYSHAGVF LHNAAEQWY DITNGQWDT 
      QANVMVESG MFDLFVMIG PSPEDLVQR YLSLTGTFR 
      LPQLWTLGY HQSRWSYMS QEEVKDVVA NFTTYNFPL 
      DVIWLDVDY TDGKKYFTW DPATYSDPV EMQKNISAA 
      FKRLVAIID PHIKVEKGY NVYDGALEK GYFVKRANG 
      SVFEGDCWP GLSSYIDFL NPDARQYYG SYYSYDKFP 
      YSTPVLSGI WNDMNEPSV FDNSIEKTL PGDSLHFGN 
      VSHRDIHNI YGLLHTMST HQGLLDRDN GTTRPFILT 
      RAHFAGTQR YSGIWTGDN TAGWGYLSV SYDSCLGAN 
      LLGLVFCGA DVGGFSGNP DTELLQRWY QAGAWLPFY 
      RAHASSDTQ RREPYLFDS GVQGVIRGA IQMRYQHLP 
      VWYTLFYEH ERNKVPVIR PLFYHYSYE LETFKLRNH 
      LLVGRDILV RAVAEPGVE TVTVHFPGS ENEHWMPVD 
      STEVYEGTT DVDVPVDIK SIPVFYRVG SVIVRKDLV 
      RLSTDEMAN DGYTINACL DRRNQSTGT VFLDDFTSY 
      NYANNKQYN YLRIDMDDV NVALTKIDE DSNPDGFDF 
      VIDEVIIHR IVQEPQNGK PGRYVKTVH KTDANGQLL 
      KNLKFNYDE PVSIKLGL
    • >TC031427 RB
      MIPTFTFIE IIDGVLQQD NYVTIQVGE TTAQIFYDP 
      FVVNFYLVQ VHQVVIDGS TLTFDESLD QAFAFNVKF 
      PAALQLYGL HEHADSLAL RDTNGSDPY RLRNLDVAG 
      YELDSPMAL YGAVPVLYG WGTYSHAGV FLHNAAEQW 
      YDITNGQWD TQANVMVES GMFDLFVMI GPSPEDLVQ 
      RYLSLTGTF RLPQLWTLG YHQSRWSYM SQEEVKDVV 
      ANFTTYNFP LDVIWLDVD YTDGKKYFT WDPATYSDP 
      VEMQKNISA AFKRLVAII DPHIKVEKG YNVYDGALE 
      KGYFVKRAN GSVFEGDCW PGLSSYIDF LNPDARQYY 
      GSYYSYDKF PYSTPVLSG IWNDMNEPS VFDNSIEKT 
      LPGDSLHFG NVSHRDIHN IYGLLHTMS THQGLLDRD 
      NGTTRPFIL TRAHFAGTQ RYSGIWTGD NTAGWGYLS 
      VSYDSCLGA NLLGLVFCG ADVGGFSGN PDTELLQRW 
      YQAGAWLPF YRAHASSDT QRREPYLFD SGVQGVIRG 
      AIQMRYQHL PVWYTLFYE HERNKVPVI RPLFYHYSY 
      ELETFKLRN HLLVGRDIL VRAVAEPGV ETVTVHFPG 
      SENEHWMPV DSTEVYEGT TDVDVPVDI KSIPVFYRV 
      GSVIVRKDL VRLSTDEMA NDGYTINAC LDRRNQSTG 
      TVFLDDFTS YNYANNKQY NYLRIDMDD VNVALTKID 
      EDSNPDGFD FVIDEVIIH RIVQEPQNG KPGRYVKTV 
      HKTDANGQL LKNLKFNYD EPVSIKLGL
    • >TC031427 RC
      MLRLVILAT CLTLGHFAD HDTFKNCSR VEFCNTLRN 
      RQPFDKYAV DPSTITIDD NGSVKMTLK AKKGSDLQL 
      ELLALVDRT FRLRIKETA STRYELHDV LVAEPQLAQ
    CDS sequence
    • >TC031427 RA
      atgttaaga ctagttatt ctagccacg tgcttaacc 
      cttggccat tttgccgac catgacact ttcaaaaac 
      tgctcccgt gtggaattt tgcaatacg ttacgaaac 
      cgccaaccc ttcgacaaa tacgcagtc gacccctca 
      accatcaca atcgacgac aatggcagc gtaaaaatg 
      actctaaaa gcaaaaaaa ggctccgac ctccaactg 
      gaactcctg gccctagtc gaccgcacc ttccgcctg 
      cgaataaaa gaaacagct tcgacacga tacgaactc 
      cacgacgtt ctggtcgcc gagccccaa ctagcccac 
      attgacggc gtcttgcaa caagacaat tacgtgacc 
      atccaagtt ggcgagacc accgcccag attttctac 
      gacccgttc gttgtcaac ttctacttg gtgcaagtg 
      caccaggtg gtgattgac ggtagtacc ttgactttt 
      gacgagtcg ctggaccaa gcgttcgca ttcaatgtc 
      aaattcccg gcggctttg caactttac gggcttcat 
      gagcatgcc gatagcttg gcgttgcgg gacaccaat 
      gggtcggac ccatacagg ctgcggaac ctggacgtg 
      gccgggtac gagctggac tcgcccatg gcgctgtat 
      ggggcggtg cctgtgctg tatggatgg gggacttat 
      tcccatgcg ggcgttttc ctgcacaat gcggccgag 
      caatggtac gacattacc aacggccaa tgggacacc 
      caagccaac gtgatggtc gaaagtggg atgtttgac 
      ctgtttgtg atgatcggg ccttcgccc gaggacttg 
      gtccagcgc tatttgagt ctcactggt actttccgt 
      ctaccacag ttgtggact ttagggtac caccagtca 
      cgctggagt tacatgagc caggaggaa gtcaaggac 
      gtcgtggcc aactttacc acgtacaat ttccccttg 
      gacgtgatc tggcttgat gttgattac acagatggg 
      aagaagtac tttacctgg gacccagca acttacagc 
      gatccggtg gaaatgcag aaaaacatc tcagcggcg 
      ttcaaacgc ctggtggca atcatagac ccacatatt 
      aaggttgaa aaagggtac aacgtgtat gacggggct 
      ttggaaaaa gggtacttt gtgaagcgg gccaatggc 
      agtgttttt gaaggcgat tgttggccg ggcttgagc 
      agttacatt gattttctg aacccggac gcccgtcag 
      tactacggt agttactat tcgtacgat aaattcccg 
      tactcgacc ccggtactg tccggtatt tggaacgac 
      atgaacgaa ccgtccgtg tttgacaac agtatcgaa 
      aaaaccctt ccgggggat tccctccac tttggtaac 
      gtctcccac cgcgatatc cacaacatc tacggcctg 
      cttcacacc atgtcgacc catcaaggc ctactagac 
      cgtgataac ggtaccact cgccccttt atcctcaca 
      cgggcgcac tttgctggt actcagagg tactccggt 
      atctggacg ggggacaac acagctgga tgggggtac 
      ttgagcgtc agttacgac agttgtctt ggtgctaat 
      ctcctgggg ctggtgttt tgtggggct gatgttggg 
      gggttcagt ggtaatcct gatacggag cttttgcaa 
      aggtggtac caggcgggc gcttggttg ccgttctac 
      cgcgctcac gcgagctcg gatacgcaa aggcgggag 
      ccttatctt tttgacagt ggggtgcag ggggttatc 
      aggggggcg attcaaatg aggtaccag catttgcca 
      gtttggtac actttgttc tacgagcat gagaggaac 
      aaagtaccg gttataagg ccccttttt tatcactat 
      tcgtacgaa ttggagact tttaaacta aggaaccac 
      cttctagta gggagggac attctagtg cgagctgtt 
      gccgaaccg ggggttgag acagtaact gttcatttc 
      ccaggtagc gagaatgaa cactggatg ccagttgat 
      agtaccgaa gtttacgaa ggaactacc gatgttgat 
      gttccagtt gatataaaa tcgattcca gttttctac 
      cgagtgggg agtgtcata gttcggaaa gacctagtt 
      agactaagt accgacgaa atggctaat gatggttac 
      accattaat gcctgttta gacaggagg aaccaatca 
      actggcacc gtgtttttg gacgacttc acctcgtat 
      aattacgcc aataacaag cagtacaac tatttgagg 
      attgacatg gatgacgtg aacgtggca ctaacgaaa 
      atcgacgag gattctaat cctgacggg tttgatttt 
      gttattgac gaagttata atacatcgg attgtgcag 
      gagccacaa aatggaaaa ccgggacgg tacgttaaa 
      accgttcac aaaactgat gccaatgga cagctgctc 
      aaaaatttg aagtttaat tatgatgaa cctgttagt 
      ataaagctt ggattgtga
    • >TC031427 RB
      atgataccc acctttact tttattgaa atcattgac 
      ggcgtcttg caacaagac aattacgtg accatccaa 
      gttggcgag accaccgcc cagattttc tacgacccg 
      ttcgttgtc aacttctac ttggtgcaa gtgcaccag 
      gtggtgatt gacggtagt accttgact tttgacgag 
      tcgctggac caagcgttc gcattcaat gtcaaattc 
      ccggcggct ttgcaactt tacgggctt catgagcat 
      gccgatagc ttggcgttg cgggacacc aatgggtcg 
      gacccatac aggctgcgg aacctggac gtggccggg 
      tacgagctg gactcgccc atggcgctg tatggggcg 
      gtgcctgtg ctgtatgga tgggggact tattcccat 
      gcgggcgtt ttcctgcac aatgcggcc gagcaatgg 
      tacgacatt accaacggc caatgggac acccaagcc 
      aacgtgatg gtcgaaagt gggatgttt gacctgttt 
      gtgatgatc gggccttcg cccgaggac ttggtccag 
      cgctatttg agtctcact ggtactttc cgtctacca 
      cagttgtgg actttaggg taccaccag tcacgctgg 
      agttacatg agccaggag gaagtcaag gacgtcgtg 
      gccaacttt accacgtac aatttcccc ttggacgtg 
      atctggctt gatgttgat tacacagat gggaagaag 
      tactttacc tgggaccca gcaacttac agcgatccg 
      gtggaaatg cagaaaaac atctcagcg gcgttcaaa 
      cgcctggtg gcaatcata gacccacat attaaggtt 
      gaaaaaggg tacaacgtg tatgacggg gctttggaa 
      aaagggtac tttgtgaag cgggccaat ggcagtgtt 
      tttgaaggc gattgttgg ccgggcttg agcagttac 
      attgatttt ctgaacccg gacgcccgt cagtactac 
      ggtagttac tattcgtac gataaattc ccgtactcg 
      accccggta ctgtccggt atttggaac gacatgaac 
      gaaccgtcc gtgtttgac aacagtatc gaaaaaacc 
      cttccgggg gattccctc cactttggt aacgtctcc 
      caccgcgat atccacaac atctacggc ctgcttcac 
      accatgtcg acccatcaa ggcctacta gaccgtgat 
      aacggtacc actcgcccc tttatcctc acacgggcg 
      cactttgct ggtactcag aggtactcc ggtatctgg 
      acgggggac aacacagct ggatggggg tacttgagc 
      gtcagttac gacagttgt cttggtgct aatctcctg 
      gggctggtg ttttgtggg gctgatgtt ggggggttc 
      agtggtaat cctgatacg gagcttttg caaaggtgg 
      taccaggcg ggcgcttgg ttgccgttc taccgcgct 
      cacgcgagc tcggatacg caaaggcgg gagccttat 
      ctttttgac agtggggtg cagggggtt atcaggggg 
      gcgattcaa atgaggtac cagcatttg ccagtttgg 
      tacactttg ttctacgag catgagagg aacaaagta 
      ccggttata aggcccctt ttttatcac tattcgtac 
      gaattggag acttttaaa ctaaggaac caccttcta 
      gtagggagg gacattcta gtgcgagct gttgccgaa 
      ccgggggtt gagacagta actgttcat ttcccaggt 
      agcgagaat gaacactgg atgccagtt gatagtacc 
      gaagtttac gaaggaact accgatgtt gatgttcca 
      gttgatata aaatcgatt ccagttttc taccgagtg 
      gggagtgtc atagttcgg aaagaccta gttagacta 
      agtaccgac gaaatggct aatgatggt tacaccatt 
      aatgcctgt ttagacagg aggaaccaa tcaactggc 
      accgtgttt ttggacgac ttcacctcg tataattac 
      gccaataac aagcagtac aactatttg aggattgac 
      atggatgac gtgaacgtg gcactaacg aaaatcgac 
      gaggattct aatcctgac gggtttgat tttgttatt 
      gacgaagtt ataatacat cggattgtg caggagcca 
      caaaatgga aaaccggga cggtacgtt aaaaccgtt 
      cacaaaact gatgccaat ggacagctg ctcaaaaat 
      ttgaagttt aattatgat gaacctgtt agtataaag 
      cttggattg tga
    • >TC031427 RC
      atgttaaga ctagttatt ctagccacg tgcttaacc 
      cttggccat tttgccgac catgacact ttcaaaaac 
      tgctcccgt gtggaattt tgcaatacg ttacgaaac 
      cgccaaccc ttcgacaaa tacgcagtc gacccctca 
      accatcaca atcgacgac aatggcagc gtaaaaatg 
      actctaaaa gcaaaaaaa ggctccgac ctccaactg 
      gaactcctg gccctagtc gaccgcacc ttccgcctg 
      cgaataaaa gaaacagct tcgacacga tacgaactc 
      cacgacgtt ctggtcgcc gagccccaa ctagcccag 
      taa
    mRNA sequence
    • >TC031427 RA
      tctatctaa acccacaag catgttaag actagttat 
      tctagccac gtgcttaac ccttggcca ttttgccga 
      ccatgacac tttcaaaaa ctgctcccg tgtggaatt 
      ttgcaatac gttacgaaa ccgccaacc cttcgacaa 
      atacgcagt cgacccctc aaccatcac aatcgacga 
      caatggcag cgtaaaaat gactctaaa agcaaaaaa 
      aggctccga cctccaact ggaactcct ggccctagt 
      cgaccgcac cttccgcct gcgaataaa agaaacagc 
      ttcgacacg atacgaact ccacgacgt tctggtcgc 
      cgagcccca actagccca cattgacgg cgtcttgca 
      acaagacaa ttacgtgac catccaagt tggcgagac 
      caccgccca gattttcta cgacccgtt cgttgtcaa 
      cttctactt ggtgcaagt gcaccaggt ggtgattga 
      cggtagtac cttgacttt tgacgagtc gctggacca 
      agcgttcgc attcaatgt caaattccc ggcggcttt 
      gcaacttta cgggcttca tgagcatgc cgatagctt 
      ggcgttgcg ggacaccaa tgggtcgga cccatacag 
      gctgcggaa cctggacgt ggccgggta cgagctgga 
      ctcgcccat ggcgctgta tggggcggt gcctgtgct 
      gtatggatg ggggactta ttcccatgc gggcgtttt 
      cctgcacaa tgcggccga gcaatggta cgacattac 
      caacggcca atgggacac ccaagccaa cgtgatggt 
      cgaaagtgg gatgtttga cctgtttgt gatgatcgg 
      gccttcgcc cgaggactt ggtccagcg ctatttgag 
      tctcactgg tactttccg tctaccaca gttgtggac 
      tttagggta ccaccagtc acgctggag ttacatgag 
      ccaggagga agtcaagga cgtcgtggc caactttac 
      cacgtacaa tttcccctt ggacgtgat ctggcttga 
      tgttgatta cacagatgg gaagaagta ctttacctg 
      ggacccagc aacttacag cgatccggt ggaaatgca 
      gaaaaacat ctcagcggc gttcaaacg cctggtggc 
      aatcataga cccacatat taaggttga aaaagggta 
      caacgtgta tgacggggc tttggaaaa agggtactt 
      tgtgaagcg ggccaatgg cagtgtttt tgaaggcga 
      ttgttggcc gggcttgag cagttacat tgattttct 
      gaacccgga cgcccgtca gtactacgg tagttacta 
      ttcgtacga taaattccc gtactcgac cccggtact 
      gtccggtat ttggaacga catgaacga accgtccgt 
      gtttgacaa cagtatcga aaaaaccct tccggggga 
      ttccctcca ctttggtaa cgtctccca ccgcgatat 
      ccacaacat ctacggcct gcttcacac catgtcgac 
      ccatcaagg cctactaga ccgtgataa cggtaccac 
      tcgcccctt tatcctcac acgggcgca ctttgctgg 
      tactcagag gtactccgg tatctggac gggggacaa 
      cacagctgg atgggggta cttgagcgt cagttacga 
      cagttgtct tggtgctaa tctcctggg gctggtgtt 
      ttgtggggc tgatgttgg ggggttcag tggtaatcc 
      tgatacgga gcttttgca aaggtggta ccaggcggg 
      cgcttggtt gccgttcta ccgcgctca cgcgagctc 
      ggatacgca aaggcggga gccttatct ttttgacag 
      tggggtgca gggggttat caggggggc gattcaaat 
      gaggtacca gcatttgcc agtttggta cactttgtt 
      ctacgagca tgagaggaa caaagtacc ggttataag 
      gcccctttt ttatcacta ttcgtacga attggagac 
      ttttaaact aaggaacca ccttctagt agggaggga 
      cattctagt gcgagctgt tgccgaacc gggggttga 
      gacagtaac tgttcattt cccaggtag cgagaatga 
      acactggat gccagttga tagtaccga agtttacga 
      aggaactac cgatgttga tgttccagt tgatataaa 
      atcgattcc agttttcta ccgagtggg gagtgtcat 
      agttcggaa agacctagt tagactaag taccgacga 
      aatggctaa tgatggtta caccattaa tgcctgttt 
      agacaggag gaaccaatc aactggcac cgtgttttt 
      ggacgactt cacctcgta taattacgc caataacaa 
      gcagtacaa ctatttgag gattgacat ggatgacgt 
      gaacgtggc actaacgaa aatcgacga ggattctaa 
      tcctgacgg gtttgattt tgttattga cgaagttat 
      aatacatcg gattgtgca ggagccaca aaatggaaa 
      accgggacg gtacgttaa aaccgttca caaaactga 
      tgccaatgg acagctgct caaaaattt gaagtttaa 
      ttatgatga acctgttag tataaagct tggattgtg 
      attgtgtcg tttcaataa actgtaatt atctga
    • >TC031427 RB
      aaatatttt cagaacggt ttaacagaa aaccttgaa 
      atttgggga acagttcaa gtgtttaaa ttctatcat 
      ttgagagct ttttcaaat ttttatcgt catttattt 
      tgaaaattc aaggctaac ttgattttt tttaaatgg 
      cacgaaagg tcatttttt aaagatcat ttttttctg 
      aattcaatg atgaaacat gatacccac ctttacttt 
      tattgaaat cattgacgg cgtcttgca acaagacaa 
      ttacgtgac catccaagt tggcgagac caccgccca 
      gattttcta cgacccgtt cgttgtcaa cttctactt 
      ggtgcaagt gcaccaggt ggtgattga cggtagtac 
      cttgacttt tgacgagtc gctggacca agcgttcgc 
      attcaatgt caaattccc ggcggcttt gcaacttta 
      cgggcttca tgagcatgc cgatagctt ggcgttgcg 
      ggacaccaa tgggtcgga cccatacag gctgcggaa 
      cctggacgt ggccgggta cgagctgga ctcgcccat 
      ggcgctgta tggggcggt gcctgtgct gtatggatg 
      ggggactta ttcccatgc gggcgtttt cctgcacaa 
      tgcggccga gcaatggta cgacattac caacggcca 
      atgggacac ccaagccaa cgtgatggt cgaaagtgg 
      gatgtttga cctgtttgt gatgatcgg gccttcgcc 
      cgaggactt ggtccagcg ctatttgag tctcactgg 
      tactttccg tctaccaca gttgtggac tttagggta 
      ccaccagtc acgctggag ttacatgag ccaggagga 
      agtcaagga cgtcgtggc caactttac cacgtacaa 
      tttcccctt ggacgtgat ctggcttga tgttgatta 
      cacagatgg gaagaagta ctttacctg ggacccagc 
      aacttacag cgatccggt ggaaatgca gaaaaacat 
      ctcagcggc gttcaaacg cctggtggc aatcataga 
      cccacatat taaggttga aaaagggta caacgtgta 
      tgacggggc tttggaaaa agggtactt tgtgaagcg 
      ggccaatgg cagtgtttt tgaaggcga ttgttggcc 
      gggcttgag cagttacat tgattttct gaacccgga 
      cgcccgtca gtactacgg tagttacta ttcgtacga 
      taaattccc gtactcgac cccggtact gtccggtat 
      ttggaacga catgaacga accgtccgt gtttgacaa 
      cagtatcga aaaaaccct tccggggga ttccctcca 
      ctttggtaa cgtctccca ccgcgatat ccacaacat 
      ctacggcct gcttcacac catgtcgac ccatcaagg 
      cctactaga ccgtgataa cggtaccac tcgcccctt 
      tatcctcac acgggcgca ctttgctgg tactcagag 
      gtactccgg tatctggac gggggacaa cacagctgg 
      atgggggta cttgagcgt cagttacga cagttgtct 
      tggtgctaa tctcctggg gctggtgtt ttgtggggc 
      tgatgttgg ggggttcag tggtaatcc tgatacgga 
      gcttttgca aaggtggta ccaggcggg cgcttggtt 
      gccgttcta ccgcgctca cgcgagctc ggatacgca 
      aaggcggga gccttatct ttttgacag tggggtgca 
      gggggttat caggggggc gattcaaat gaggtacca 
      gcatttgcc agtttggta cactttgtt ctacgagca 
      tgagaggaa caaagtacc ggttataag gcccctttt 
      ttatcacta ttcgtacga attggagac ttttaaact 
      aaggaacca ccttctagt agggaggga cattctagt 
      gcgagctgt tgccgaacc gggggttga gacagtaac 
      tgttcattt cccaggtag cgagaatga acactggat 
      gccagttga tagtaccga agtttacga aggaactac 
      cgatgttga tgttccagt tgatataaa atcgattcc 
      agttttcta ccgagtggg gagtgtcat agttcggaa 
      agacctagt tagactaag taccgacga aatggctaa 
      tgatggtta caccattaa tgcctgttt agacaggag 
      gaaccaatc aactggcac cgtgttttt ggacgactt 
      cacctcgta taattacgc caataacaa gcagtacaa 
      ctatttgag gattgacat ggatgacgt gaacgtggc 
      actaacgaa aatcgacga ggattctaa tcctgacgg 
      gtttgattt tgttattga cgaagttat aatacatcg 
      gattgtgca ggagccaca aaatggaaa accgggacg 
      gtacgttaa aaccgttca caaaactga tgccaatgg 
      acagctgct caaaaattt gaagtttaa ttatgatga 
      acctgttag tataaagct tggattgtg attgtgtcg 
      tttcaataa actgtaatt atctga
    • >TC031427 RC
      tctatctaa acccacaag catgttaag actagttat 
      tctagccac gtgcttaac ccttggcca ttttgccga 
      ccatgacac tttcaaaaa ctgctcccg tgtggaatt 
      ttgcaatac gttacgaaa ccgccaacc cttcgacaa 
      atacgcagt cgacccctc aaccatcac aatcgacga 
      caatggcag cgtaaaaat gactctaaa agcaaaaaa 
      aggctccga cctccaact ggaactcct ggccctagt 
      cgaccgcac cttccgcct gcgaataaa agaaacagc 
      ttcgacacg atacgaact ccacgacgt tctggtcgc 
      cgagcccca actagccca gtaagttgc agtactggg 
      ataagtacc acctaagca agcttccag caacaagac 
      aattacgtg accatccaa gttggcgag accaccgcc 
      cagattttc tacgagccg ttcgttgtc aacttttac 
      ctggtgcaa gtgcaccag gtggtgatt gactaataa 
      gattttaca tttgtcaaa attttcgat ttgagagct 
      ttttcaaat tgtta
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