General information | |||
TC031427
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 4336797..4345933 | |
Sequence information | |||
Transcripts / Proteins (3) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC031427 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02558 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_06363 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)
- >TC031427 RA
MLRLVILAT CLTLGHFAD HDTFKNCSR VEFCNTLRN RQPFDKYAV DPSTITIDD NGSVKMTLK AKKGSDLQL ELLALVDRT FRLRIKETA STRYELHDV LVAEPQLAH IDGVLQQDN YVTIQVGET TAQIFYDPF VVNFYLVQV HQVVIDGST LTFDESLDQ AFAFNVKFP AALQLYGLH EHADSLALR DTNGSDPYR LRNLDVAGY ELDSPMALY GAVPVLYGW GTYSHAGVF LHNAAEQWY DITNGQWDT QANVMVESG MFDLFVMIG PSPEDLVQR YLSLTGTFR LPQLWTLGY HQSRWSYMS QEEVKDVVA NFTTYNFPL DVIWLDVDY TDGKKYFTW DPATYSDPV EMQKNISAA FKRLVAIID PHIKVEKGY NVYDGALEK GYFVKRANG SVFEGDCWP GLSSYIDFL NPDARQYYG SYYSYDKFP YSTPVLSGI WNDMNEPSV FDNSIEKTL PGDSLHFGN VSHRDIHNI YGLLHTMST HQGLLDRDN GTTRPFILT RAHFAGTQR YSGIWTGDN TAGWGYLSV SYDSCLGAN LLGLVFCGA DVGGFSGNP DTELLQRWY QAGAWLPFY RAHASSDTQ RREPYLFDS GVQGVIRGA IQMRYQHLP VWYTLFYEH ERNKVPVIR PLFYHYSYE LETFKLRNH LLVGRDILV RAVAEPGVE TVTVHFPGS ENEHWMPVD STEVYEGTT DVDVPVDIK SIPVFYRVG SVIVRKDLV RLSTDEMAN DGYTINACL DRRNQSTGT VFLDDFTSY NYANNKQYN YLRIDMDDV NVALTKIDE DSNPDGFDF VIDEVIIHR IVQEPQNGK PGRYVKTVH KTDANGQLL KNLKFNYDE PVSIKLGL
- >TC031427 RB
MIPTFTFIE IIDGVLQQD NYVTIQVGE TTAQIFYDP FVVNFYLVQ VHQVVIDGS TLTFDESLD QAFAFNVKF PAALQLYGL HEHADSLAL RDTNGSDPY RLRNLDVAG YELDSPMAL YGAVPVLYG WGTYSHAGV FLHNAAEQW YDITNGQWD TQANVMVES GMFDLFVMI GPSPEDLVQ RYLSLTGTF RLPQLWTLG YHQSRWSYM SQEEVKDVV ANFTTYNFP LDVIWLDVD YTDGKKYFT WDPATYSDP VEMQKNISA AFKRLVAII DPHIKVEKG YNVYDGALE KGYFVKRAN GSVFEGDCW PGLSSYIDF LNPDARQYY GSYYSYDKF PYSTPVLSG IWNDMNEPS VFDNSIEKT LPGDSLHFG NVSHRDIHN IYGLLHTMS THQGLLDRD NGTTRPFIL TRAHFAGTQ RYSGIWTGD NTAGWGYLS VSYDSCLGA NLLGLVFCG ADVGGFSGN PDTELLQRW YQAGAWLPF YRAHASSDT QRREPYLFD SGVQGVIRG AIQMRYQHL PVWYTLFYE HERNKVPVI RPLFYHYSY ELETFKLRN HLLVGRDIL VRAVAEPGV ETVTVHFPG SENEHWMPV DSTEVYEGT TDVDVPVDI KSIPVFYRV GSVIVRKDL VRLSTDEMA NDGYTINAC LDRRNQSTG TVFLDDFTS YNYANNKQY NYLRIDMDD VNVALTKID EDSNPDGFD FVIDEVIIH RIVQEPQNG KPGRYVKTV HKTDANGQL LKNLKFNYD EPVSIKLGL
- >TC031427 RC
MLRLVILAT CLTLGHFAD HDTFKNCSR VEFCNTLRN RQPFDKYAV DPSTITIDD NGSVKMTLK AKKGSDLQL ELLALVDRT FRLRIKETA STRYELHDV LVAEPQLAQ
- >TC031427 RA
atgttaaga ctagttatt ctagccacg tgcttaacc cttggccat tttgccgac catgacact ttcaaaaac tgctcccgt gtggaattt tgcaatacg ttacgaaac cgccaaccc ttcgacaaa tacgcagtc gacccctca accatcaca atcgacgac aatggcagc gtaaaaatg actctaaaa gcaaaaaaa ggctccgac ctccaactg gaactcctg gccctagtc gaccgcacc ttccgcctg cgaataaaa gaaacagct tcgacacga tacgaactc cacgacgtt ctggtcgcc gagccccaa ctagcccac attgacggc gtcttgcaa caagacaat tacgtgacc atccaagtt ggcgagacc accgcccag attttctac gacccgttc gttgtcaac ttctacttg gtgcaagtg caccaggtg gtgattgac ggtagtacc ttgactttt gacgagtcg ctggaccaa gcgttcgca ttcaatgtc aaattcccg gcggctttg caactttac gggcttcat gagcatgcc gatagcttg gcgttgcgg gacaccaat gggtcggac ccatacagg ctgcggaac ctggacgtg gccgggtac gagctggac tcgcccatg gcgctgtat ggggcggtg cctgtgctg tatggatgg gggacttat tcccatgcg ggcgttttc ctgcacaat gcggccgag caatggtac gacattacc aacggccaa tgggacacc caagccaac gtgatggtc gaaagtggg atgtttgac ctgtttgtg atgatcggg ccttcgccc gaggacttg gtccagcgc tatttgagt ctcactggt actttccgt ctaccacag ttgtggact ttagggtac caccagtca cgctggagt tacatgagc caggaggaa gtcaaggac gtcgtggcc aactttacc acgtacaat ttccccttg gacgtgatc tggcttgat gttgattac acagatggg aagaagtac tttacctgg gacccagca acttacagc gatccggtg gaaatgcag aaaaacatc tcagcggcg ttcaaacgc ctggtggca atcatagac ccacatatt aaggttgaa aaagggtac aacgtgtat gacggggct ttggaaaaa gggtacttt gtgaagcgg gccaatggc agtgttttt gaaggcgat tgttggccg ggcttgagc agttacatt gattttctg aacccggac gcccgtcag tactacggt agttactat tcgtacgat aaattcccg tactcgacc ccggtactg tccggtatt tggaacgac atgaacgaa ccgtccgtg tttgacaac agtatcgaa aaaaccctt ccgggggat tccctccac tttggtaac gtctcccac cgcgatatc cacaacatc tacggcctg cttcacacc atgtcgacc catcaaggc ctactagac cgtgataac ggtaccact cgccccttt atcctcaca cgggcgcac tttgctggt actcagagg tactccggt atctggacg ggggacaac acagctgga tgggggtac ttgagcgtc agttacgac agttgtctt ggtgctaat ctcctgggg ctggtgttt tgtggggct gatgttggg gggttcagt ggtaatcct gatacggag cttttgcaa aggtggtac caggcgggc gcttggttg ccgttctac cgcgctcac gcgagctcg gatacgcaa aggcgggag ccttatctt tttgacagt ggggtgcag ggggttatc aggggggcg attcaaatg aggtaccag catttgcca gtttggtac actttgttc tacgagcat gagaggaac aaagtaccg gttataagg ccccttttt tatcactat tcgtacgaa ttggagact tttaaacta aggaaccac cttctagta gggagggac attctagtg cgagctgtt gccgaaccg ggggttgag acagtaact gttcatttc ccaggtagc gagaatgaa cactggatg ccagttgat agtaccgaa gtttacgaa ggaactacc gatgttgat gttccagtt gatataaaa tcgattcca gttttctac cgagtgggg agtgtcata gttcggaaa gacctagtt agactaagt accgacgaa atggctaat gatggttac accattaat gcctgttta gacaggagg aaccaatca actggcacc gtgtttttg gacgacttc acctcgtat aattacgcc aataacaag cagtacaac tatttgagg attgacatg gatgacgtg aacgtggca ctaacgaaa atcgacgag gattctaat cctgacggg tttgatttt gttattgac gaagttata atacatcgg attgtgcag gagccacaa aatggaaaa ccgggacgg tacgttaaa accgttcac aaaactgat gccaatgga cagctgctc aaaaatttg aagtttaat tatgatgaa cctgttagt ataaagctt ggattgtga
- >TC031427 RB
atgataccc acctttact tttattgaa atcattgac ggcgtcttg caacaagac aattacgtg accatccaa gttggcgag accaccgcc cagattttc tacgacccg ttcgttgtc aacttctac ttggtgcaa gtgcaccag gtggtgatt gacggtagt accttgact tttgacgag tcgctggac caagcgttc gcattcaat gtcaaattc ccggcggct ttgcaactt tacgggctt catgagcat gccgatagc ttggcgttg cgggacacc aatgggtcg gacccatac aggctgcgg aacctggac gtggccggg tacgagctg gactcgccc atggcgctg tatggggcg gtgcctgtg ctgtatgga tgggggact tattcccat gcgggcgtt ttcctgcac aatgcggcc gagcaatgg tacgacatt accaacggc caatgggac acccaagcc aacgtgatg gtcgaaagt gggatgttt gacctgttt gtgatgatc gggccttcg cccgaggac ttggtccag cgctatttg agtctcact ggtactttc cgtctacca cagttgtgg actttaggg taccaccag tcacgctgg agttacatg agccaggag gaagtcaag gacgtcgtg gccaacttt accacgtac aatttcccc ttggacgtg atctggctt gatgttgat tacacagat gggaagaag tactttacc tgggaccca gcaacttac agcgatccg gtggaaatg cagaaaaac atctcagcg gcgttcaaa cgcctggtg gcaatcata gacccacat attaaggtt gaaaaaggg tacaacgtg tatgacggg gctttggaa aaagggtac tttgtgaag cgggccaat ggcagtgtt tttgaaggc gattgttgg ccgggcttg agcagttac attgatttt ctgaacccg gacgcccgt cagtactac ggtagttac tattcgtac gataaattc ccgtactcg accccggta ctgtccggt atttggaac gacatgaac gaaccgtcc gtgtttgac aacagtatc gaaaaaacc cttccgggg gattccctc cactttggt aacgtctcc caccgcgat atccacaac atctacggc ctgcttcac accatgtcg acccatcaa ggcctacta gaccgtgat aacggtacc actcgcccc tttatcctc acacgggcg cactttgct ggtactcag aggtactcc ggtatctgg acgggggac aacacagct ggatggggg tacttgagc gtcagttac gacagttgt cttggtgct aatctcctg gggctggtg ttttgtggg gctgatgtt ggggggttc agtggtaat cctgatacg gagcttttg caaaggtgg taccaggcg ggcgcttgg ttgccgttc taccgcgct cacgcgagc tcggatacg caaaggcgg gagccttat ctttttgac agtggggtg cagggggtt atcaggggg gcgattcaa atgaggtac cagcatttg ccagtttgg tacactttg ttctacgag catgagagg aacaaagta ccggttata aggcccctt ttttatcac tattcgtac gaattggag acttttaaa ctaaggaac caccttcta gtagggagg gacattcta gtgcgagct gttgccgaa ccgggggtt gagacagta actgttcat ttcccaggt agcgagaat gaacactgg atgccagtt gatagtacc gaagtttac gaaggaact accgatgtt gatgttcca gttgatata aaatcgatt ccagttttc taccgagtg gggagtgtc atagttcgg aaagaccta gttagacta agtaccgac gaaatggct aatgatggt tacaccatt aatgcctgt ttagacagg aggaaccaa tcaactggc accgtgttt ttggacgac ttcacctcg tataattac gccaataac aagcagtac aactatttg aggattgac atggatgac gtgaacgtg gcactaacg aaaatcgac gaggattct aatcctgac gggtttgat tttgttatt gacgaagtt ataatacat cggattgtg caggagcca caaaatgga aaaccggga cggtacgtt aaaaccgtt cacaaaact gatgccaat ggacagctg ctcaaaaat ttgaagttt aattatgat gaacctgtt agtataaag cttggattg tga
- >TC031427 RC
atgttaaga ctagttatt ctagccacg tgcttaacc cttggccat tttgccgac catgacact ttcaaaaac tgctcccgt gtggaattt tgcaatacg ttacgaaac cgccaaccc ttcgacaaa tacgcagtc gacccctca accatcaca atcgacgac aatggcagc gtaaaaatg actctaaaa gcaaaaaaa ggctccgac ctccaactg gaactcctg gccctagtc gaccgcacc ttccgcctg cgaataaaa gaaacagct tcgacacga tacgaactc cacgacgtt ctggtcgcc gagccccaa ctagcccag taa
- >TC031427 RA
tctatctaa acccacaag catgttaag actagttat tctagccac gtgcttaac ccttggcca ttttgccga ccatgacac tttcaaaaa ctgctcccg tgtggaatt ttgcaatac gttacgaaa ccgccaacc cttcgacaa atacgcagt cgacccctc aaccatcac aatcgacga caatggcag cgtaaaaat gactctaaa agcaaaaaa aggctccga cctccaact ggaactcct ggccctagt cgaccgcac cttccgcct gcgaataaa agaaacagc ttcgacacg atacgaact ccacgacgt tctggtcgc cgagcccca actagccca cattgacgg cgtcttgca acaagacaa ttacgtgac catccaagt tggcgagac caccgccca gattttcta cgacccgtt cgttgtcaa cttctactt ggtgcaagt gcaccaggt ggtgattga cggtagtac cttgacttt tgacgagtc gctggacca agcgttcgc attcaatgt caaattccc ggcggcttt gcaacttta cgggcttca tgagcatgc cgatagctt ggcgttgcg ggacaccaa tgggtcgga cccatacag gctgcggaa cctggacgt ggccgggta cgagctgga ctcgcccat ggcgctgta tggggcggt gcctgtgct gtatggatg ggggactta ttcccatgc gggcgtttt cctgcacaa tgcggccga gcaatggta cgacattac caacggcca atgggacac ccaagccaa cgtgatggt cgaaagtgg gatgtttga cctgtttgt gatgatcgg gccttcgcc cgaggactt ggtccagcg ctatttgag tctcactgg tactttccg tctaccaca gttgtggac tttagggta ccaccagtc acgctggag ttacatgag ccaggagga agtcaagga cgtcgtggc caactttac cacgtacaa tttcccctt ggacgtgat ctggcttga tgttgatta cacagatgg gaagaagta ctttacctg ggacccagc aacttacag cgatccggt ggaaatgca gaaaaacat ctcagcggc gttcaaacg cctggtggc aatcataga cccacatat taaggttga aaaagggta caacgtgta tgacggggc tttggaaaa agggtactt tgtgaagcg ggccaatgg cagtgtttt tgaaggcga ttgttggcc gggcttgag cagttacat tgattttct gaacccgga cgcccgtca gtactacgg tagttacta ttcgtacga taaattccc gtactcgac cccggtact gtccggtat ttggaacga catgaacga accgtccgt gtttgacaa cagtatcga aaaaaccct tccggggga ttccctcca ctttggtaa cgtctccca ccgcgatat ccacaacat ctacggcct gcttcacac catgtcgac ccatcaagg cctactaga ccgtgataa cggtaccac tcgcccctt tatcctcac acgggcgca ctttgctgg tactcagag gtactccgg tatctggac gggggacaa cacagctgg atgggggta cttgagcgt cagttacga cagttgtct tggtgctaa tctcctggg gctggtgtt ttgtggggc tgatgttgg ggggttcag tggtaatcc tgatacgga gcttttgca aaggtggta ccaggcggg cgcttggtt gccgttcta ccgcgctca cgcgagctc ggatacgca aaggcggga gccttatct ttttgacag tggggtgca gggggttat caggggggc gattcaaat gaggtacca gcatttgcc agtttggta cactttgtt ctacgagca tgagaggaa caaagtacc ggttataag gcccctttt ttatcacta ttcgtacga attggagac ttttaaact aaggaacca ccttctagt agggaggga cattctagt gcgagctgt tgccgaacc gggggttga gacagtaac tgttcattt cccaggtag cgagaatga acactggat gccagttga tagtaccga agtttacga aggaactac cgatgttga tgttccagt tgatataaa atcgattcc agttttcta ccgagtggg gagtgtcat agttcggaa agacctagt tagactaag taccgacga aatggctaa tgatggtta caccattaa tgcctgttt agacaggag gaaccaatc aactggcac cgtgttttt ggacgactt cacctcgta taattacgc caataacaa gcagtacaa ctatttgag gattgacat ggatgacgt gaacgtggc actaacgaa aatcgacga ggattctaa tcctgacgg gtttgattt tgttattga cgaagttat aatacatcg gattgtgca ggagccaca aaatggaaa accgggacg gtacgttaa aaccgttca caaaactga tgccaatgg acagctgct caaaaattt gaagtttaa ttatgatga acctgttag tataaagct tggattgtg attgtgtcg tttcaataa actgtaatt atctga
- >TC031427 RB
aaatatttt cagaacggt ttaacagaa aaccttgaa atttgggga acagttcaa gtgtttaaa ttctatcat ttgagagct ttttcaaat ttttatcgt catttattt tgaaaattc aaggctaac ttgattttt tttaaatgg cacgaaagg tcatttttt aaagatcat ttttttctg aattcaatg atgaaacat gatacccac ctttacttt tattgaaat cattgacgg cgtcttgca acaagacaa ttacgtgac catccaagt tggcgagac caccgccca gattttcta cgacccgtt cgttgtcaa cttctactt ggtgcaagt gcaccaggt ggtgattga cggtagtac cttgacttt tgacgagtc gctggacca agcgttcgc attcaatgt caaattccc ggcggcttt gcaacttta cgggcttca tgagcatgc cgatagctt ggcgttgcg ggacaccaa tgggtcgga cccatacag gctgcggaa cctggacgt ggccgggta cgagctgga ctcgcccat ggcgctgta tggggcggt gcctgtgct gtatggatg ggggactta ttcccatgc gggcgtttt cctgcacaa tgcggccga gcaatggta cgacattac caacggcca atgggacac ccaagccaa cgtgatggt cgaaagtgg gatgtttga cctgtttgt gatgatcgg gccttcgcc cgaggactt ggtccagcg ctatttgag tctcactgg tactttccg tctaccaca gttgtggac tttagggta ccaccagtc acgctggag ttacatgag ccaggagga agtcaagga cgtcgtggc caactttac cacgtacaa tttcccctt ggacgtgat ctggcttga tgttgatta cacagatgg gaagaagta ctttacctg ggacccagc aacttacag cgatccggt ggaaatgca gaaaaacat ctcagcggc gttcaaacg cctggtggc aatcataga cccacatat taaggttga aaaagggta caacgtgta tgacggggc tttggaaaa agggtactt tgtgaagcg ggccaatgg cagtgtttt tgaaggcga ttgttggcc gggcttgag cagttacat tgattttct gaacccgga cgcccgtca gtactacgg tagttacta ttcgtacga taaattccc gtactcgac cccggtact gtccggtat ttggaacga catgaacga accgtccgt gtttgacaa cagtatcga aaaaaccct tccggggga ttccctcca ctttggtaa cgtctccca ccgcgatat ccacaacat ctacggcct gcttcacac catgtcgac ccatcaagg cctactaga ccgtgataa cggtaccac tcgcccctt tatcctcac acgggcgca ctttgctgg tactcagag gtactccgg tatctggac gggggacaa cacagctgg atgggggta cttgagcgt cagttacga cagttgtct tggtgctaa tctcctggg gctggtgtt ttgtggggc tgatgttgg ggggttcag tggtaatcc tgatacgga gcttttgca aaggtggta ccaggcggg cgcttggtt gccgttcta ccgcgctca cgcgagctc ggatacgca aaggcggga gccttatct ttttgacag tggggtgca gggggttat caggggggc gattcaaat gaggtacca gcatttgcc agtttggta cactttgtt ctacgagca tgagaggaa caaagtacc ggttataag gcccctttt ttatcacta ttcgtacga attggagac ttttaaact aaggaacca ccttctagt agggaggga cattctagt gcgagctgt tgccgaacc gggggttga gacagtaac tgttcattt cccaggtag cgagaatga acactggat gccagttga tagtaccga agtttacga aggaactac cgatgttga tgttccagt tgatataaa atcgattcc agttttcta ccgagtggg gagtgtcat agttcggaa agacctagt tagactaag taccgacga aatggctaa tgatggtta caccattaa tgcctgttt agacaggag gaaccaatc aactggcac cgtgttttt ggacgactt cacctcgta taattacgc caataacaa gcagtacaa ctatttgag gattgacat ggatgacgt gaacgtggc actaacgaa aatcgacga ggattctaa tcctgacgg gtttgattt tgttattga cgaagttat aatacatcg gattgtgca ggagccaca aaatggaaa accgggacg gtacgttaa aaccgttca caaaactga tgccaatgg acagctgct caaaaattt gaagtttaa ttatgatga acctgttag tataaagct tggattgtg attgtgtcg tttcaataa actgtaatt atctga
- >TC031427 RC
tctatctaa acccacaag catgttaag actagttat tctagccac gtgcttaac ccttggcca ttttgccga ccatgacac tttcaaaaa ctgctcccg tgtggaatt ttgcaatac gttacgaaa ccgccaacc cttcgacaa atacgcagt cgacccctc aaccatcac aatcgacga caatggcag cgtaaaaat gactctaaa agcaaaaaa aggctccga cctccaact ggaactcct ggccctagt cgaccgcac cttccgcct gcgaataaa agaaacagc ttcgacacg atacgaact ccacgacgt tctggtcgc cgagcccca actagccca gtaagttgc agtactggg ataagtacc acctaagca agcttccag caacaagac aattacgtg accatccaa gttggcgag accaccgcc cagattttc tacgagccg ttcgttgtc aacttttac ctggtgcaa gtgcaccag gtggtgatt gactaataa gattttaca tttgtcaaa attttcgat ttgagagct ttttcaaat tgtta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
