Status
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Gene information
General information
TC031427
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016218 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4336797..4345933
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031427
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02558

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06363

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031427 RA
      MLRLVILAT CLTLGHFAD HDTFKNCSR VEFCNTLRN 
RQPFDKYAV DPSTITIDD NGSVKMTLK AKKGSDLQL 
ELLALVDRT FRLRIKETA STRYELHDV LVAEPQLAH 
IDGVLQQDN YVTIQVGET TAQIFYDPF VVNFYLVQV 
HQVVIDGST LTFDESLDQ AFAFNVKFP AALQLYGLH 
EHADSLALR DTNGSDPYR LRNLDVAGY ELDSPMALY 
GAVPVLYGW GTYSHAGVF LHNAAEQWY DITNGQWDT 
QANVMVESG MFDLFVMIG PSPEDLVQR YLSLTGTFR 
LPQLWTLGY HQSRWSYMS QEEVKDVVA NFTTYNFPL 
DVIWLDVDY TDGKKYFTW DPATYSDPV EMQKNISAA 
FKRLVAIID PHIKVEKGY NVYDGALEK GYFVKRANG 
SVFEGDCWP GLSSYIDFL NPDARQYYG SYYSYDKFP 
YSTPVLSGI WNDMNEPSV FDNSIEKTL PGDSLHFGN 
VSHRDIHNI YGLLHTMST HQGLLDRDN GTTRPFILT 
RAHFAGTQR YSGIWTGDN TAGWGYLSV SYDSCLGAN 
LLGLVFCGA DVGGFSGNP DTELLQRWY QAGAWLPFY 
RAHASSDTQ RREPYLFDS GVQGVIRGA IQMRYQHLP 
VWYTLFYEH ERNKVPVIR PLFYHYSYE LETFKLRNH 
LLVGRDILV RAVAEPGVE TVTVHFPGS ENEHWMPVD 
STEVYEGTT DVDVPVDIK SIPVFYRVG SVIVRKDLV 
RLSTDEMAN DGYTINACL DRRNQSTGT VFLDDFTSY 
NYANNKQYN YLRIDMDDV NVALTKIDE DSNPDGFDF 
VIDEVIIHR IVQEPQNGK PGRYVKTVH KTDANGQLL 
KNLKFNYDE PVSIKLGL
    • >TC031427 RB
      MIPTFTFIE IIDGVLQQD NYVTIQVGE TTAQIFYDP 
FVVNFYLVQ VHQVVIDGS TLTFDESLD QAFAFNVKF 
PAALQLYGL HEHADSLAL RDTNGSDPY RLRNLDVAG 
YELDSPMAL YGAVPVLYG WGTYSHAGV FLHNAAEQW 
YDITNGQWD TQANVMVES GMFDLFVMI GPSPEDLVQ 
RYLSLTGTF RLPQLWTLG YHQSRWSYM SQEEVKDVV 
ANFTTYNFP LDVIWLDVD YTDGKKYFT WDPATYSDP 
VEMQKNISA AFKRLVAII DPHIKVEKG YNVYDGALE 
KGYFVKRAN GSVFEGDCW PGLSSYIDF LNPDARQYY 
GSYYSYDKF PYSTPVLSG IWNDMNEPS VFDNSIEKT 
LPGDSLHFG NVSHRDIHN IYGLLHTMS THQGLLDRD 
NGTTRPFIL TRAHFAGTQ RYSGIWTGD NTAGWGYLS 
VSYDSCLGA NLLGLVFCG ADVGGFSGN PDTELLQRW 
YQAGAWLPF YRAHASSDT QRREPYLFD SGVQGVIRG 
AIQMRYQHL PVWYTLFYE HERNKVPVI RPLFYHYSY 
ELETFKLRN HLLVGRDIL VRAVAEPGV ETVTVHFPG 
SENEHWMPV DSTEVYEGT TDVDVPVDI KSIPVFYRV 
GSVIVRKDL VRLSTDEMA NDGYTINAC LDRRNQSTG 
TVFLDDFTS YNYANNKQY NYLRIDMDD VNVALTKID 
EDSNPDGFD FVIDEVIIH RIVQEPQNG KPGRYVKTV 
HKTDANGQL LKNLKFNYD EPVSIKLGL
    • >TC031427 RC
      MLRLVILAT CLTLGHFAD HDTFKNCSR VEFCNTLRN 
RQPFDKYAV DPSTITIDD NGSVKMTLK AKKGSDLQL 
ELLALVDRT FRLRIKETA STRYELHDV LVAEPQLAQ
    CDS sequence
    • >TC031427 RA
      atgttaaga ctagttatt ctagccacg tgcttaacc 
cttggccat tttgccgac catgacact ttcaaaaac 
tgctcccgt gtggaattt tgcaatacg ttacgaaac 
cgccaaccc ttcgacaaa tacgcagtc gacccctca 
accatcaca atcgacgac aatggcagc gtaaaaatg 
actctaaaa gcaaaaaaa ggctccgac ctccaactg 
gaactcctg gccctagtc gaccgcacc ttccgcctg 
cgaataaaa gaaacagct tcgacacga tacgaactc 
cacgacgtt ctggtcgcc gagccccaa ctagcccac 
attgacggc gtcttgcaa caagacaat tacgtgacc 
atccaagtt ggcgagacc accgcccag attttctac 
gacccgttc gttgtcaac ttctacttg gtgcaagtg 
caccaggtg gtgattgac ggtagtacc ttgactttt 
gacgagtcg ctggaccaa gcgttcgca ttcaatgtc 
aaattcccg gcggctttg caactttac gggcttcat 
gagcatgcc gatagcttg gcgttgcgg gacaccaat 
gggtcggac ccatacagg ctgcggaac ctggacgtg 
gccgggtac gagctggac tcgcccatg gcgctgtat 
ggggcggtg cctgtgctg tatggatgg gggacttat 
tcccatgcg ggcgttttc ctgcacaat gcggccgag 
caatggtac gacattacc aacggccaa tgggacacc 
caagccaac gtgatggtc gaaagtggg atgtttgac 
ctgtttgtg atgatcggg ccttcgccc gaggacttg 
gtccagcgc tatttgagt ctcactggt actttccgt 
ctaccacag ttgtggact ttagggtac caccagtca 
cgctggagt tacatgagc caggaggaa gtcaaggac 
gtcgtggcc aactttacc acgtacaat ttccccttg 
gacgtgatc tggcttgat gttgattac acagatggg 
aagaagtac tttacctgg gacccagca acttacagc 
gatccggtg gaaatgcag aaaaacatc tcagcggcg 
ttcaaacgc ctggtggca atcatagac ccacatatt 
aaggttgaa aaagggtac aacgtgtat gacggggct 
ttggaaaaa gggtacttt gtgaagcgg gccaatggc 
agtgttttt gaaggcgat tgttggccg ggcttgagc 
agttacatt gattttctg aacccggac gcccgtcag 
tactacggt agttactat tcgtacgat aaattcccg 
tactcgacc ccggtactg tccggtatt tggaacgac 
atgaacgaa ccgtccgtg tttgacaac agtatcgaa 
aaaaccctt ccgggggat tccctccac tttggtaac 
gtctcccac cgcgatatc cacaacatc tacggcctg 
cttcacacc atgtcgacc catcaaggc ctactagac 
cgtgataac ggtaccact cgccccttt atcctcaca 
cgggcgcac tttgctggt actcagagg tactccggt 
atctggacg ggggacaac acagctgga tgggggtac 
ttgagcgtc agttacgac agttgtctt ggtgctaat 
ctcctgggg ctggtgttt tgtggggct gatgttggg 
gggttcagt ggtaatcct gatacggag cttttgcaa 
aggtggtac caggcgggc gcttggttg ccgttctac 
cgcgctcac gcgagctcg gatacgcaa aggcgggag 
ccttatctt tttgacagt ggggtgcag ggggttatc 
aggggggcg attcaaatg aggtaccag catttgcca 
gtttggtac actttgttc tacgagcat gagaggaac 
aaagtaccg gttataagg ccccttttt tatcactat 
tcgtacgaa ttggagact tttaaacta aggaaccac 
cttctagta gggagggac attctagtg cgagctgtt 
gccgaaccg ggggttgag acagtaact gttcatttc 
ccaggtagc gagaatgaa cactggatg ccagttgat 
agtaccgaa gtttacgaa ggaactacc gatgttgat 
gttccagtt gatataaaa tcgattcca gttttctac 
cgagtgggg agtgtcata gttcggaaa gacctagtt 
agactaagt accgacgaa atggctaat gatggttac 
accattaat gcctgttta gacaggagg aaccaatca 
actggcacc gtgtttttg gacgacttc acctcgtat 
aattacgcc aataacaag cagtacaac tatttgagg 
attgacatg gatgacgtg aacgtggca ctaacgaaa 
atcgacgag gattctaat cctgacggg tttgatttt 
gttattgac gaagttata atacatcgg attgtgcag 
gagccacaa aatggaaaa ccgggacgg tacgttaaa 
accgttcac aaaactgat gccaatgga cagctgctc 
aaaaatttg aagtttaat tatgatgaa cctgttagt 
ataaagctt ggattgtga
    • >TC031427 RB
      atgataccc acctttact tttattgaa atcattgac 
ggcgtcttg caacaagac aattacgtg accatccaa 
gttggcgag accaccgcc cagattttc tacgacccg 
ttcgttgtc aacttctac ttggtgcaa gtgcaccag 
gtggtgatt gacggtagt accttgact tttgacgag 
tcgctggac caagcgttc gcattcaat gtcaaattc 
ccggcggct ttgcaactt tacgggctt catgagcat 
gccgatagc ttggcgttg cgggacacc aatgggtcg 
gacccatac aggctgcgg aacctggac gtggccggg 
tacgagctg gactcgccc atggcgctg tatggggcg 
gtgcctgtg ctgtatgga tgggggact tattcccat 
gcgggcgtt ttcctgcac aatgcggcc gagcaatgg 
tacgacatt accaacggc caatgggac acccaagcc 
aacgtgatg gtcgaaagt gggatgttt gacctgttt 
gtgatgatc gggccttcg cccgaggac ttggtccag 
cgctatttg agtctcact ggtactttc cgtctacca 
cagttgtgg actttaggg taccaccag tcacgctgg 
agttacatg agccaggag gaagtcaag gacgtcgtg 
gccaacttt accacgtac aatttcccc ttggacgtg 
atctggctt gatgttgat tacacagat gggaagaag 
tactttacc tgggaccca gcaacttac agcgatccg 
gtggaaatg cagaaaaac atctcagcg gcgttcaaa 
cgcctggtg gcaatcata gacccacat attaaggtt 
gaaaaaggg tacaacgtg tatgacggg gctttggaa 
aaagggtac tttgtgaag cgggccaat ggcagtgtt 
tttgaaggc gattgttgg ccgggcttg agcagttac 
attgatttt ctgaacccg gacgcccgt cagtactac 
ggtagttac tattcgtac gataaattc ccgtactcg 
accccggta ctgtccggt atttggaac gacatgaac 
gaaccgtcc gtgtttgac aacagtatc gaaaaaacc 
cttccgggg gattccctc cactttggt aacgtctcc 
caccgcgat atccacaac atctacggc ctgcttcac 
accatgtcg acccatcaa ggcctacta gaccgtgat 
aacggtacc actcgcccc tttatcctc acacgggcg 
cactttgct ggtactcag aggtactcc ggtatctgg 
acgggggac aacacagct ggatggggg tacttgagc 
gtcagttac gacagttgt cttggtgct aatctcctg 
gggctggtg ttttgtggg gctgatgtt ggggggttc 
agtggtaat cctgatacg gagcttttg caaaggtgg 
taccaggcg ggcgcttgg ttgccgttc taccgcgct 
cacgcgagc tcggatacg caaaggcgg gagccttat 
ctttttgac agtggggtg cagggggtt atcaggggg 
gcgattcaa atgaggtac cagcatttg ccagtttgg 
tacactttg ttctacgag catgagagg aacaaagta 
ccggttata aggcccctt ttttatcac tattcgtac 
gaattggag acttttaaa ctaaggaac caccttcta 
gtagggagg gacattcta gtgcgagct gttgccgaa 
ccgggggtt gagacagta actgttcat ttcccaggt 
agcgagaat gaacactgg atgccagtt gatagtacc 
gaagtttac gaaggaact accgatgtt gatgttcca 
gttgatata aaatcgatt ccagttttc taccgagtg 
gggagtgtc atagttcgg aaagaccta gttagacta 
agtaccgac gaaatggct aatgatggt tacaccatt 
aatgcctgt ttagacagg aggaaccaa tcaactggc 
accgtgttt ttggacgac ttcacctcg tataattac 
gccaataac aagcagtac aactatttg aggattgac 
atggatgac gtgaacgtg gcactaacg aaaatcgac 
gaggattct aatcctgac gggtttgat tttgttatt 
gacgaagtt ataatacat cggattgtg caggagcca 
caaaatgga aaaccggga cggtacgtt aaaaccgtt 
cacaaaact gatgccaat ggacagctg ctcaaaaat 
ttgaagttt aattatgat gaacctgtt agtataaag 
cttggattg tga
    • >TC031427 RC
      atgttaaga ctagttatt ctagccacg tgcttaacc 
cttggccat tttgccgac catgacact ttcaaaaac 
tgctcccgt gtggaattt tgcaatacg ttacgaaac 
cgccaaccc ttcgacaaa tacgcagtc gacccctca 
accatcaca atcgacgac aatggcagc gtaaaaatg 
actctaaaa gcaaaaaaa ggctccgac ctccaactg 
gaactcctg gccctagtc gaccgcacc ttccgcctg 
cgaataaaa gaaacagct tcgacacga tacgaactc 
cacgacgtt ctggtcgcc gagccccaa ctagcccag 
taa
    mRNA sequence
    • >TC031427 RA
      tctatctaa acccacaag catgttaag actagttat 
tctagccac gtgcttaac ccttggcca ttttgccga 
ccatgacac tttcaaaaa ctgctcccg tgtggaatt 
ttgcaatac gttacgaaa ccgccaacc cttcgacaa 
atacgcagt cgacccctc aaccatcac aatcgacga 
caatggcag cgtaaaaat gactctaaa agcaaaaaa 
aggctccga cctccaact ggaactcct ggccctagt 
cgaccgcac cttccgcct gcgaataaa agaaacagc 
ttcgacacg atacgaact ccacgacgt tctggtcgc 
cgagcccca actagccca cattgacgg cgtcttgca 
acaagacaa ttacgtgac catccaagt tggcgagac 
caccgccca gattttcta cgacccgtt cgttgtcaa 
cttctactt ggtgcaagt gcaccaggt ggtgattga 
cggtagtac cttgacttt tgacgagtc gctggacca 
agcgttcgc attcaatgt caaattccc ggcggcttt 
gcaacttta cgggcttca tgagcatgc cgatagctt 
ggcgttgcg ggacaccaa tgggtcgga cccatacag 
gctgcggaa cctggacgt ggccgggta cgagctgga 
ctcgcccat ggcgctgta tggggcggt gcctgtgct 
gtatggatg ggggactta ttcccatgc gggcgtttt 
cctgcacaa tgcggccga gcaatggta cgacattac 
caacggcca atgggacac ccaagccaa cgtgatggt 
cgaaagtgg gatgtttga cctgtttgt gatgatcgg 
gccttcgcc cgaggactt ggtccagcg ctatttgag 
tctcactgg tactttccg tctaccaca gttgtggac 
tttagggta ccaccagtc acgctggag ttacatgag 
ccaggagga agtcaagga cgtcgtggc caactttac 
cacgtacaa tttcccctt ggacgtgat ctggcttga 
tgttgatta cacagatgg gaagaagta ctttacctg 
ggacccagc aacttacag cgatccggt ggaaatgca 
gaaaaacat ctcagcggc gttcaaacg cctggtggc 
aatcataga cccacatat taaggttga aaaagggta 
caacgtgta tgacggggc tttggaaaa agggtactt 
tgtgaagcg ggccaatgg cagtgtttt tgaaggcga 
ttgttggcc gggcttgag cagttacat tgattttct 
gaacccgga cgcccgtca gtactacgg tagttacta 
ttcgtacga taaattccc gtactcgac cccggtact 
gtccggtat ttggaacga catgaacga accgtccgt 
gtttgacaa cagtatcga aaaaaccct tccggggga 
ttccctcca ctttggtaa cgtctccca ccgcgatat 
ccacaacat ctacggcct gcttcacac catgtcgac 
ccatcaagg cctactaga ccgtgataa cggtaccac 
tcgcccctt tatcctcac acgggcgca ctttgctgg 
tactcagag gtactccgg tatctggac gggggacaa 
cacagctgg atgggggta cttgagcgt cagttacga 
cagttgtct tggtgctaa tctcctggg gctggtgtt 
ttgtggggc tgatgttgg ggggttcag tggtaatcc 
tgatacgga gcttttgca aaggtggta ccaggcggg 
cgcttggtt gccgttcta ccgcgctca cgcgagctc 
ggatacgca aaggcggga gccttatct ttttgacag 
tggggtgca gggggttat caggggggc gattcaaat 
gaggtacca gcatttgcc agtttggta cactttgtt 
ctacgagca tgagaggaa caaagtacc ggttataag 
gcccctttt ttatcacta ttcgtacga attggagac 
ttttaaact aaggaacca ccttctagt agggaggga 
cattctagt gcgagctgt tgccgaacc gggggttga 
gacagtaac tgttcattt cccaggtag cgagaatga 
acactggat gccagttga tagtaccga agtttacga 
aggaactac cgatgttga tgttccagt tgatataaa 
atcgattcc agttttcta ccgagtggg gagtgtcat 
agttcggaa agacctagt tagactaag taccgacga 
aatggctaa tgatggtta caccattaa tgcctgttt 
agacaggag gaaccaatc aactggcac cgtgttttt 
ggacgactt cacctcgta taattacgc caataacaa 
gcagtacaa ctatttgag gattgacat ggatgacgt 
gaacgtggc actaacgaa aatcgacga ggattctaa 
tcctgacgg gtttgattt tgttattga cgaagttat 
aatacatcg gattgtgca ggagccaca aaatggaaa 
accgggacg gtacgttaa aaccgttca caaaactga 
tgccaatgg acagctgct caaaaattt gaagtttaa 
ttatgatga acctgttag tataaagct tggattgtg 
attgtgtcg tttcaataa actgtaatt atctga
    • >TC031427 RB
      aaatatttt cagaacggt ttaacagaa aaccttgaa 
atttgggga acagttcaa gtgtttaaa ttctatcat 
ttgagagct ttttcaaat ttttatcgt catttattt 
tgaaaattc aaggctaac ttgattttt tttaaatgg 
cacgaaagg tcatttttt aaagatcat ttttttctg 
aattcaatg atgaaacat gatacccac ctttacttt 
tattgaaat cattgacgg cgtcttgca acaagacaa 
ttacgtgac catccaagt tggcgagac caccgccca 
gattttcta cgacccgtt cgttgtcaa cttctactt 
ggtgcaagt gcaccaggt ggtgattga cggtagtac 
cttgacttt tgacgagtc gctggacca agcgttcgc 
attcaatgt caaattccc ggcggcttt gcaacttta 
cgggcttca tgagcatgc cgatagctt ggcgttgcg 
ggacaccaa tgggtcgga cccatacag gctgcggaa 
cctggacgt ggccgggta cgagctgga ctcgcccat 
ggcgctgta tggggcggt gcctgtgct gtatggatg 
ggggactta ttcccatgc gggcgtttt cctgcacaa 
tgcggccga gcaatggta cgacattac caacggcca 
atgggacac ccaagccaa cgtgatggt cgaaagtgg 
gatgtttga cctgtttgt gatgatcgg gccttcgcc 
cgaggactt ggtccagcg ctatttgag tctcactgg 
tactttccg tctaccaca gttgtggac tttagggta 
ccaccagtc acgctggag ttacatgag ccaggagga 
agtcaagga cgtcgtggc caactttac cacgtacaa 
tttcccctt ggacgtgat ctggcttga tgttgatta 
cacagatgg gaagaagta ctttacctg ggacccagc 
aacttacag cgatccggt ggaaatgca gaaaaacat 
ctcagcggc gttcaaacg cctggtggc aatcataga 
cccacatat taaggttga aaaagggta caacgtgta 
tgacggggc tttggaaaa agggtactt tgtgaagcg 
ggccaatgg cagtgtttt tgaaggcga ttgttggcc 
gggcttgag cagttacat tgattttct gaacccgga 
cgcccgtca gtactacgg tagttacta ttcgtacga 
taaattccc gtactcgac cccggtact gtccggtat 
ttggaacga catgaacga accgtccgt gtttgacaa 
cagtatcga aaaaaccct tccggggga ttccctcca 
ctttggtaa cgtctccca ccgcgatat ccacaacat 
ctacggcct gcttcacac catgtcgac ccatcaagg 
cctactaga ccgtgataa cggtaccac tcgcccctt 
tatcctcac acgggcgca ctttgctgg tactcagag 
gtactccgg tatctggac gggggacaa cacagctgg 
atgggggta cttgagcgt cagttacga cagttgtct 
tggtgctaa tctcctggg gctggtgtt ttgtggggc 
tgatgttgg ggggttcag tggtaatcc tgatacgga 
gcttttgca aaggtggta ccaggcggg cgcttggtt 
gccgttcta ccgcgctca cgcgagctc ggatacgca 
aaggcggga gccttatct ttttgacag tggggtgca 
gggggttat caggggggc gattcaaat gaggtacca 
gcatttgcc agtttggta cactttgtt ctacgagca 
tgagaggaa caaagtacc ggttataag gcccctttt 
ttatcacta ttcgtacga attggagac ttttaaact 
aaggaacca ccttctagt agggaggga cattctagt 
gcgagctgt tgccgaacc gggggttga gacagtaac 
tgttcattt cccaggtag cgagaatga acactggat 
gccagttga tagtaccga agtttacga aggaactac 
cgatgttga tgttccagt tgatataaa atcgattcc 
agttttcta ccgagtggg gagtgtcat agttcggaa 
agacctagt tagactaag taccgacga aatggctaa 
tgatggtta caccattaa tgcctgttt agacaggag 
gaaccaatc aactggcac cgtgttttt ggacgactt 
cacctcgta taattacgc caataacaa gcagtacaa 
ctatttgag gattgacat ggatgacgt gaacgtggc 
actaacgaa aatcgacga ggattctaa tcctgacgg 
gtttgattt tgttattga cgaagttat aatacatcg 
gattgtgca ggagccaca aaatggaaa accgggacg 
gtacgttaa aaccgttca caaaactga tgccaatgg 
acagctgct caaaaattt gaagtttaa ttatgatga 
acctgttag tataaagct tggattgtg attgtgtcg 
tttcaataa actgtaatt atctga
    • >TC031427 RC
      tctatctaa acccacaag catgttaag actagttat 
tctagccac gtgcttaac ccttggcca ttttgccga 
ccatgacac tttcaaaaa ctgctcccg tgtggaatt 
ttgcaatac gttacgaaa ccgccaacc cttcgacaa 
atacgcagt cgacccctc aaccatcac aatcgacga 
caatggcag cgtaaaaat gactctaaa agcaaaaaa 
aggctccga cctccaact ggaactcct ggccctagt 
cgaccgcac cttccgcct gcgaataaa agaaacagc 
ttcgacacg atacgaact ccacgacgt tctggtcgc 
cgagcccca actagccca gtaagttgc agtactggg 
ataagtacc acctaagca agcttccag caacaagac 
aattacgtg accatccaa gttggcgag accaccgcc 
cagattttc tacgagccg ttcgttgtc aacttttac 
ctggtgcaa gtgcaccag gtggtgatt gactaataa 
gattttaca tttgtcaaa attttcgat ttgagagct 
ttttcaaat tgtta
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