Status
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Gene information
General information
TC016216 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4319487..4324192
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016216
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02557

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    iBeetle screen iB_07114

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016216 RA
      MSSLRNGSS ESSSDSSTD GEDQDESSS SVTQGVEDV 
      LSTFREKWQ KELQISPPK REHQSQDTP QDPDAETQA 
      RRLFLKGVD LEKSGKLYE SIQFYRRAV QLVPDIEFR 
      IDKEEMHEK DEENSEGEH EVTREGELL QRIQKKLSK 
      RPALCVPKT EQKGTHISV LPTEMILHI LRWVVSAEL 
      DLRSLEMFS MVCRGFYLC ARDPEVWRL ACLRVWGLN 
      CGNSPYNYL SWRHMFIER TRLHFNGCY IGKTTYIRH 
      GENNFQDQF YRPWHLVAY YRYLRFFPE GVVLVLTSS 
      EEPAQCVSL MKSRNARSP ILRGYYRLK DDKVTLVVQ 
      RQDNKHVPG FKKKRRGGD LREMAEQTF HMELQIKST 
      NKKRHVQLV WTHYSVFTR THKGDESTC NFDLVGNRF 
      PPFWFSRVK SYTSESDSP LQ
    CDS sequence
    • >TC016216 RA
      atgagttct cttagaaat ggctcgagt gagtcttca 
      tcagactcc tcaacggac ggtgaagac caggacgag 
      tcttcttcc tcggtcacc cagggcgtg gaggacgtc 
      ctgagcacc ttccgggag aaatggcag aaggagctc 
      cagatatcg ccgccaaaa cgcgaacac cagtcgcag 
      gacacgccc caggacccg gacgccgag acccaggcc 
      aggagactg ttcctcaag ggcgtggac ctggaaaaa 
      tccgggaaa ctgtacgag tccatccag ttctaccga 
      cgcgcggtc cagctggtg cccgacata gagttccgc 
      attgacaag gaggagatg cacgagaaa gatgaagaa 
      aactccgaa ggcgagcac gaagtaacg cgcgagggc 
      gagctcctg cagcgcatt cagaaaaaa ctgtccaaa 
      aggccggcc ctttgcgtg ccaaaaacc gagcagaaa 
      ggcacgcac atctcagtc ctgccaacg gaaatgatt 
      ttgcacatt ttgcggtgg gtggtgagt gcggagctg 
      gatttgagg tcgctggag atgttttcg atggtgtgt 
      cggggcttt tatttgtgc gcacgtgac ccggaggtg 
      tggcggttg gcgtgcctg agggtctgg ggcttgaac 
      tgcgggaat tccccgtat aattacctg tcttggcgg 
      cacatgttt atcgagcgg acgcggctg catttcaac 
      ggctgctat attgggaag acgacgtac atcaggcac 
      ggggagaac aatttccag gaccagttt taccggccg 
      tggcatcta gtggcctat taccgctac ttgcggttt 
      ttccccgag ggggtggtg ctggttttg acgtcgtcg 
      gaggagccg gcgcagtgt gtgagtctg atgaagagc 
      aggaacgct cggagcccc attctgaga ggctattac 
      cggctgaag gacgataaa gtcacgctg gttgtgcaa 
      agacaggat aataagcac gtgccaggc tttaagaag 
      aagcggaga ggcggggat ttgcgggaa atggcggag 
      cagacgttt cacatggaa ttacagatt aaaagtacc 
      aataaaaaa cgccatgtg cagttggtt tggacacat 
      tactccgtt tttacaagg acgcacaaa ggtgatgag 
      agtacctgc aattttgac ttggtcggt aaccgattt 
      ccgccattt tggttctcg agagtcaag agctacact 
      tcagagtct gatagtcct ttacagtag
    mRNA sequence
    • >TC016216 RA
      tcaacaatc agcgtttta gtgcaaaac atggaagtt 
      cagctttgt aataattcc caaaatgtt acaattgta 
      gttagatat acagaccgt gaactaatc tcccgcatc 
      taaattggt caaagaatg agttctctt agaaatggc 
      tcgagtgag tcttcatca gactcctca acggacggt 
      gaagaccag gacgagtct tcttcctcg gtcacccag 
      ggcgtggag gacgtcctg agcaccttc cgggagaaa 
      tggcagaag gagctccag atatcgccg ccaaaacgc 
      gaacaccag tcgcaggac acgccccag gacccggac 
      gccgagacc caggccagg agactgttc ctcaagggc 
      gtggacctg gaaaaatcc gggaaactg tacgagtcc 
      atccagttc taccgacgc gcggtccag ctggtgccc 
      gacatagag ttccgcatt gacaaggag gagatgcac 
      gagaaagat gaagaaaac tccgaaggc gagcacgaa 
      gtaacgcgc gagggcgag ctcctgcag cgcattcag 
      aaaaaactg tccaaaagg ccggccctt tgcgtgcca 
      aaaaccgag cagaaaggc acgcacatc tcagtcctg 
      ccaacggaa atgattttg cacattttg cggtgggtg 
      gtgagtgcg gagctggat ttgaggtcg ctggagatg 
      ttttcgatg gtgtgtcgg ggcttttat ttgtgcgca 
      cgtgacccg gaggtgtgg cggttggcg tgcctgagg 
      gtctggggc ttgaactgc gggaattcc ccgtataat 
      tacctgtct tggcggcac atgtttatc gagcggacg 
      cggctgcat ttcaacggc tgctatatt gggaagacg 
      acgtacatc aggcacggg gagaacaat ttccaggac 
      cagttttac cggccgtgg catctagtg gcctattac 
      cgctacttg cggtttttc cccgagggg gtggtgctg 
      gttttgacg tcgtcggag gagccggcg cagtgtgtg 
      agtctgatg aagagcagg aacgctcgg agccccatt 
      ctgagaggc tattaccgg ctgaaggac gataaagtc 
      acgctggtt gtgcaaaga caggataat aagcacgtg 
      ccaggcttt aagaagaag cggagaggc ggggatttg 
      cgggaaatg gcggagcag acgtttcac atggaatta 
      cagattaaa agtaccaat aaaaaacgc catgtgcag 
      ttggtttgg acacattac tccgttttt acaaggacg 
      cacaaaggt gatgagagt acctgcaat tttgacttg 
      gtcggtaac cgatttccg ccattttgg ttctcgaga 
      gtcaagagc tacacttca gagtctgat agtccttta 
      cagtagagt ttttcattt ttttaagga aacgaagag 
      ggaaattat ttttggaag ggattcata ttttcgaag 
      tacttctaa tatttattt aaatatcta tacacaaaa 
      tgtacataa tttattgat aataaagga agattttat 
      tttacagag tttttattt acacaacag gaaccactt 
      acgtggtca tttaaaatt tatttttaa agaggaact 
      ttaaaacac aaacaatat caggagata caggagccg 
      gtgactttt acgcgcttg caaaatggc ggctttata 
      attcttcgc ggcattaat ttaatccgc agctaaatg 
      cgatcacgt gacctgatt gaaccaatg aagctttcc 
      gttaatgtc gccagaggc gtctaagtt ttcagagca 
      ataaattta taaaaattg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY