Status
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Gene information
General information
TC031430
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016215,TC016214 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4305857..4314817
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031430
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02556

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva appendages size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax circumference decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segmentation/division increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: the abdominal segment bounderies are more prominent than normal
    abdomen circumference decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031430 RA
      MEEYEYGKL FVGGLSWET TQDNLQRFF SRYGEVIDC 
VVMKNAESG RSRGFGFVT FSDPANVNT VLQNGPHTL 
DGRTIDPKP CNPRTLQKP KKGGGYPKV FLGGLPSNV 
TETDLRTFF TRFGKVMEV VIMYDQEKK KSRGFGFLS 
FEDEESVDR CVSEHFVNL NGKQVEIKK AEPRDGSGG 
NKMGGADPS SAWGPPQAP MGMMQGPNG QMGGPPMNL 
GAPMGPNMM QGYQGWGTS PQQQSYASY GTPSGPGSY 
QGWGAPPAP QGPPPQWGN NYGGPPQQQ GYGSYGPSA 
GSGTSWNSW NMAQNSGST GYIAGDMYT RGQSGPGGP 
ATPSAPPNM SSSGGSSKP GSDYNYGYG SYASAADAG 
GYGAPPRSY GSDGSSQVS GYSSQPPNA GAKHFNDFK 
EWPSS
    • >TC031430 RB
      MEEYEYGKL FVGGLSWET TQDNLQRFF SRYGEVIDC 
VVMKNAESG RSRGFGFVT FSDPANVNT VLQNGPHTL 
DGRTIDPKP CNPRTLQKP KKGGGYPKV FLGGLPSNV 
TETDLRTFF TRFGKVMEV VIMYDQEKK KSRGFGFLS 
FEDEESVDR CVSEHFVNL NGKQVEIKK AEPRDGSGG 
NKMGGADPS SAWGPPQAP MGMMQGPNG QMGGPPMNL 
GAPMGPNMM QGYQGWGTS PQQQSYASY GTPSGPGSY 
QGWGAPPAP QGPPPQWGN NYGGPPQQQ GYGSYGPSA 
GSGTSWNSW NMAQNSGST GPSGYIAGD MYTRGQSGP 
GGPATPSAP PNMSSSGGS SKPGSDYNY GYGSYASAA 
DAGGYGAPP RSYGSDGSS QVSGYSSQP PNAGAKHFN 
DFKEWPSS
    CDS sequence
    • >TC031430 RA
      atggaagaa tacgagtac ggcaaactc tttgtaggg 
ggcttgtct tgggagaca acccaggac aatcttcaa 
cgctttttc tctagatat ggcgaagtc atcgattgc 
gttgttatg aaaaatgca gaatcaggc cgttctaga 
ggatttggt ttcgttaca ttttcagat ccagccaat 
gtaaatacg gtacttcaa aatggccca catacacta 
gacggaaga actatcgac ccgaaacca tgtaatcca 
agaacttta caaaagccc aaaaagggc ggaggttat 
ccgaaggta ttcctaggg ggtttacct tcaaacgtg 
acggaaacc gatctgagg acgttcttc actcggttt 
ggaaaagtt atggaagtt gttattatg tatgaccag 
gagaagaaa aaatcaaga ggttttggt ttcttgtcg 
tttgaagac gaggagtca gtcgaccgc tgcgtctcc 
gaacacttc gtgaaccta aacggcaaa caagtcgag 
atcaagaaa gccgagcct cgtgacggc tccggcggt 
aataaaatg ggcggtgct gacccatcg tcagcttgg 
ggtccaccg caagcgccc atgggcatg atgcaaggt 
cccaatggt caaatgggc ggtcctccg atgaatttg 
ggcgctccg atggggccc aacatgatg cagggttac 
caaggctgg ggcacatcg ccacagcag caaagttac 
gccagttat ggtacaccg tcaggtcct ggctcgtac 
cagggatgg ggtgctccg ccggcgccg caaggaccg 
cccccacag tggggcaat aactatggg ggaccgccg 
caacagcaa ggctatggg tcgtacggt ccgagtgct 
gggagcgga acgagctgg aacagctgg aatatggcg 
cagaatagc ggatccaca ggttacatt gcaggcgac 
atgtacacc cgtggtcag tcaggcccc ggcggtccg 
gccaccccc agtgcgccg ccaaacatg tcatcgtcg 
ggcggcagc tcgaagccc ggctccgat tacaactac 
ggctacggt tcgtatgcg tcggcggcg gacgccggc 
ggctacggc gccccgccg cgctcctac ggcagcgac 
ggcagcagt caagtgagc ggatactcc tcccagcct 
ccaaacgca ggtgctaaa catttcaat gattttaaa 
gagtggccc agttcttga
    • >TC031430 RB
      atggaagaa tacgagtac ggcaaactc tttgtaggg 
ggcttgtct tgggagaca acccaggac aatcttcaa 
cgctttttc tctagatat ggcgaagtc atcgattgc 
gttgttatg aaaaatgca gaatcaggc cgttctaga 
ggatttggt ttcgttaca ttttcagat ccagccaat 
gtaaatacg gtacttcaa aatggccca catacacta 
gacggaaga actatcgac ccgaaacca tgtaatcca 
agaacttta caaaagccc aaaaagggc ggaggttat 
ccgaaggta ttcctaggg ggtttacct tcaaacgtg 
acggaaacc gatctgagg acgttcttc actcggttt 
ggaaaagtt atggaagtt gttattatg tatgaccag 
gagaagaaa aaatcaaga ggttttggt ttcttgtcg 
tttgaagac gaggagtca gtcgaccgc tgcgtctcc 
gaacacttc gtgaaccta aacggcaaa caagtcgag 
atcaagaaa gccgagcct cgtgacggc tccggcggt 
aataaaatg ggcggtgct gacccatcg tcagcttgg 
ggtccaccg caagcgccc atgggcatg atgcaaggt 
cccaatggt caaatgggc ggtcctccg atgaatttg 
ggcgctccg atggggccc aacatgatg cagggttac 
caaggctgg ggcacatcg ccacagcag caaagttac 
gccagttat ggtacaccg tcaggtcct ggctcgtac 
cagggatgg ggtgctccg ccggcgccg caaggaccg 
cccccacag tggggcaat aactatggg ggaccgccg 
caacagcaa ggctatggg tcgtacggt ccgagtgct 
gggagcgga acgagctgg aacagctgg aatatggcg 
cagaatagc ggatccaca ggtccgtca ggttacatt 
gcaggcgac atgtacacc cgtggtcag tcaggcccc 
ggcggtccg gccaccccc agtgcgccg ccaaacatg 
tcatcgtcg ggcggcagc tcgaagccc ggctccgat 
tacaactac ggctacggt tcgtatgcg tcggcggcg 
gacgccggc ggctacggc gccccgccg cgctcctac 
ggcagcgac ggcagcagt caagtgagc ggatactcc 
tcccagcct ccaaacgca ggtgctaaa catttcaat 
gattttaaa gagtggccc agttcttga
    mRNA sequence
    • >TC031430 RA
      tggccagtt tcggtgacg cagtcgttg attccatcc 
cttccctaa aatggtttc taactcccc taccctgac 
ggtacgtca tgtctgtgt attcctatc gtagttgtt 
aattaggat agttcttaa cgttagatt aattaattt 
taagtttag tgtaagaag tcattattt gtaatggaa 
gaatacgag tacggcaaa ctctttgta gggggcttg 
tcttgggag acaacccag gacaatctt caacgcttt 
ttctctaga tatggcgaa gtcatcgat tgcgttgtt 
atgaaaaat gcagaatca ggccgttct agaggattt 
ggtttcgtt acattttca gatccagcc aatgtaaat 
acggtactt caaaatggc ccacataca ctagacgga 
agaactatc gacccgaaa ccatgtaat ccaagaact 
ttacaaaag cccaaaaag ggcggaggt tatccgaag 
gtattccta gggggttta ccttcaaac gtgacggaa 
accgatctg aggacgttc ttcactcgg tttggaaaa 
gttatggaa gttgttatt atgtatgac caggagaag 
aaaaaatca agaggtttt ggtttcttg tcgtttgaa 
gacgaggag tcagtcgac cgctgcgtc tccgaacac 
ttcgtgaac ctaaacggc aaacaagtc gagatcaag 
aaagccgag cctcgtgac ggctccggc ggtaataaa 
atgggcggt gctgaccca tcgtcagct tggggtcca 
ccgcaagcg cccatgggc atgatgcaa ggtcccaat 
ggtcaaatg ggcggtcct ccgatgaat ttgggcgct 
ccgatgggg cccaacatg atgcagggt taccaaggc 
tggggcaca tcgccacag cagcaaagt tacgccagt 
tatggtaca ccgtcaggt cctggctcg taccaggga 
tggggtgct ccgccggcg ccgcaagga ccgccccca 
cagtggggc aataactat gggggaccg ccgcaacag 
caaggctat gggtcgtac ggtccgagt gctgggagc 
ggaacgagc tggaacagc tggaatatg gcgcagaat 
agcggatcc acaggttac attgcaggc gacatgtac 
acccgtggt cagtcaggc cccggcggt ccggccacc 
cccagtgcg ccgccaaac atgtcatcg tcgggcggc 
agctcgaag cccggctcc gattacaac tacggctac 
ggttcgtat gcgtcggcg gcggacgcc ggcggctac 
ggcgccccg ccgcgctcc tacggcagc gacggcagc 
agtcaagtg agcggatac tcctcccag cctccaaac 
gcaggtgct aaacatttc aatgatttt aaagagtgg 
cccagttct tgacaacct ttcgataac ggcaacaac 
agcgcatcg aacgccacc gtctcctcc tcctccacc 
aataacaac aaaaacgtg cgcttgtac gtgaatgcg 
gccgtcaaa gccaccttt tacaccgac gacgaggag 
accgtggcg ttcaagcac tacaacaac ttgagtaat 
aatttgggt atgtgttgt aacagttgg aatcgtgtt 
ggcttgatt aacatgaag agagcagcc ggactggtc 
actcccgcc aaaatcaat tattctgat tagattgac 
atatgagaa atcgacggg acacataat aacactcac 
atgcacaca caccgtctg actttcgca gctattttt 
actttttct tttatttat tgtcaggag agcacgccc 
tctaacggc agaccgaca accttgcca gaggacgct 
tccgacaca cactaacta acactacag acagacaga 
agtgttgac taattttaa gcactgctc aagttgtta 
ttaatatta attaataaa atagtgtaa attcaattc 
ttacctcat atctctatt tcaaagtta gagtgtctg 
cgtggttgc tgtggctaa caaacaaaa accgaaaaa 
aatcgagaa acgacaatg tgattctta gtttgtagg 
caaaacttc cctaagtta aatgcattt acaatagtg 
gtaataacg aacgtattt aatatttat gatgtagtc 
ctggaacgg atcacttga ttattgtaa gttgaacgt 
gctctagtc ccatgtctc aatatcaaa tcgtaggtg 
gcgtccttg tacatatac tatataaat gtattttat 
ctaagttat gcaaattga ccagcagac aatctatcc 
ataaggagt aaataattg taagttaag gtcactatt 
aataatttt atataatag cacacaacc acctagcta 
agtaggacg taagatctg gctgtacct agtaataga 
ccttgtttt gttttcttt ttttaaatt atgttacga 
cttttagtt ttaatacgc gaccgaacg tctggctat 
aatgagact ttttcaaac atcacatat caaaagttt 
gcgcttttg aaaaaggtt cgttcactt ataacggac 
gtttccgac tcgaatcat gtacatttt atacttttt 
ttttttttg taaacctat ttattctaa ttttagttc 
gattaaaat tgagttaca ccgaaactt tgaattagg 
ggttgtcag gggggaggg gcgcgattc ggattaaat 
aagctataa ctaatagat tagcagtta acgaattca 
tggtgattt atatttagt agaagtatt ctgtctgtc 
tgggcctca tgtgtctat ctaatcaag cgagtaatt 
ggttcgttt gttttaagt gatcttctt attttttat 
ttaaaaatt gagtgtgac aatctacat taagcaatg 
tttgatagc aacctagac gtaactgat attgttttc 
tagttttaa ttgtaaaat attcctcgg ttaacgcag 
accaataca attttagaa gaaaatcaa gttgaaaca 
aaaataaag cgctctttt taaa
    • >TC031430 RB
      tggccagtt tcggtgacg cagtcgttg attccatcc 
cttccctaa aatggtttc taactcccc taccctgac 
ggtacgtca tgtctgtgt attcctatc gtagttgtt 
aattaggat agttcttaa cgttagatt aattaattt 
taagtttag tgtaagaag tcattattt gtaatggaa 
gaatacgag tacggcaaa ctctttgta gggggcttg 
tcttgggag acaacccag gacaatctt caacgcttt 
ttctctaga tatggcgaa gtcatcgat tgcgttgtt 
atgaaaaat gcagaatca ggccgttct agaggattt 
ggtttcgtt acattttca gatccagcc aatgtaaat 
acggtactt caaaatggc ccacataca ctagacgga 
agaactatc gacccgaaa ccatgtaat ccaagaact 
ttacaaaag cccaaaaag ggcggaggt tatccgaag 
gtattccta gggggttta ccttcaaac gtgacggaa 
accgatctg aggacgttc ttcactcgg tttggaaaa 
gttatggaa gttgttatt atgtatgac caggagaag 
aaaaaatca agaggtttt ggtttcttg tcgtttgaa 
gacgaggag tcagtcgac cgctgcgtc tccgaacac 
ttcgtgaac ctaaacggc aaacaagtc gagatcaag 
aaagccgag cctcgtgac ggctccggc ggtaataaa 
atgggcggt gctgaccca tcgtcagct tggggtcca 
ccgcaagcg cccatgggc atgatgcaa ggtcccaat 
ggtcaaatg ggcggtcct ccgatgaat ttgggcgct 
ccgatgggg cccaacatg atgcagggt taccaaggc 
tggggcaca tcgccacag cagcaaagt tacgccagt 
tatggtaca ccgtcaggt cctggctcg taccaggga 
tggggtgct ccgccggcg ccgcaagga ccgccccca 
cagtggggc aataactat gggggaccg ccgcaacag 
caaggctat gggtcgtac ggtccgagt gctgggagc 
ggaacgagc tggaacagc tggaatatg gcgcagaat 
agcggatcc acaggtccg tcaggttac attgcaggc 
gacatgtac acccgtggt cagtcaggc cccggcggt 
ccggccacc cccagtgcg ccgccaaac atgtcatcg 
tcgggcggc agctcgaag cccggctcc gattacaac 
tacggctac ggttcgtat gcgtcggcg gcggacgcc 
ggcggctac ggcgccccg ccgcgctcc tacggcagc 
gacggcagc agtcaagtg agcggatac tcctcccag 
cctccaaac gcaggtgct aaacatttc aatgatttt 
aaagagtgg cccagttct tgacaacct ttcgataac 
ggcaacaac agcgcatcg aacgccacc gtctcctcc 
tcctccacc aataacaac aaaaacgtg cgcttgtac 
gtgaatgcg gccgtcaaa gccaccttt tacaccgac 
gacgaggag accgtggcg ttcaagcac tacaacaac 
ttgagtaat aatttgggt atgtgttgt aacagttgg 
aatcgtgtt ggcttgatt aacatgaag agagcagcc 
ggactggtc actcccgcc aaaatcaat tattctgat 
tagattgac atatgagaa atcgacggg acacataat 
aacactcac atgcacaca caccgtctg actttcgca 
gctattttt actttttct tttatttat tgtcaggag 
agcacgccc tctaacggc agaccgaca accttgcca 
gaggacgct tccgacaca cactaacta acactacag 
acagacaga agtgttgac taattttaa gcactgctc 
aagttgtta ttaatatta attaataaa atagtgtaa 
attcaattc ttacctcat atctctatt tcaaagtta 
gagtgtctg cgtggttgc tgtggctaa caaacaaaa 
accgaaaaa aatcgagaa acgacaatg tgattctta 
gtttgtagg caaaacttc cctaagtta aatgcattt 
acaatagtg gtaataacg aacgtattt aatatttat 
gatgtagtc ctggaacgg atcacttga ttattgtaa 
gttgaacgt gctctagtc ccatgtctc aatatcaaa 
tcgtaggtg gcgtccttg tacatatac tatataaat 
gtattttat ctaagttat gcaaattga ccagcagac 
aatctatcc ataaggagt aaataattg taagttaag 
gtcactatt aataatttt atataatag cacacaacc 
acctagcta agtaggacg taagatctg gctgtacct 
agtaataga ccttgtttt gttttcttt ttttaaatt 
atgttacga cttttagtt ttaatacgc gaccgaacg 
tctggctat aatgagact ttttcaaac atcacatat 
caaaagttt gcgcttttg aaaaaggtt cgttcactt 
ataacggac gtttccgac tcgaatcat gtacatttt 
atacttttt ttttttttg taaacctat ttattctaa 
ttttagttc gattaaaat tgagttaca ccgaaactt 
tgaattagg ggttgtcag gggggaggg gcgcgattc 
ggattaaat aagctataa ctaatagat tagcagtta 
acgaattca tggtgattt atatttagt agaagtatt 
ctgtctgtc tgggcctca tgtgtctat ctaatcaag 
cgagtaatt ggttcgttt gttttaagt gatcttctt 
attttttat ttaaaaatt gagtgtgac aatctacat 
taagcaatg tttgatagc aacctagac gtaactgat 
attgttttc tagttttaa ttgtaaaat attcctcgg 
ttaacgcag accaataca attttagaa gaaaatcaa 
gttgaaaca aaaataaag cgctctttt taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY