Status
Please wait ...
Gene information
General information
TC031430
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016215,TC016214
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 3176..12136
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031430
    iBeetle screen iB_02556
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    ovary without detectable defects - Number of animals showing the phenotype: 4
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larva appendages size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head partially not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head capsule shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax circumference decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segmentation/division increased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Comment: the abdominal segment bounderies are more prominent than normal
    abdomen circumference decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died with pupal and larval features) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection



    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031430 RA
      MEEYEYGKL FVGGLSWET TQDNLQRFF SRYGEVIDC 
      VVMKNAESG RSRGFGFVT FSDPANVNT VLQNGPHTL 
      DGRTIDPKP CNPRTLQKP KKGGGYPKV FLGGLPSNV 
      TETDLRTFF TRFGKVMEV VIMYDQEKK KSRGFGFLS 
      FEDEESVDR CVSEHFVNL NGKQVEIKK AEPRDGSGG 
      NKMGGADPS SAWGPPQAP MGMMQGPNG QMGGPPMNL 
      GAPMGPNMM QGYQGWGTS PQQQSYASY GTPSGPGSY 
      QGWGAPPAP QGPPPQWGN NYGGPPQQQ GYGSYGPSA 
      GSGTSWNSW NMAQNSGST GYIAGDMYT RGQSGPGGP 
      ATPSAPPNM SSSGGSSKP GSDYNYGYG SYASAADAG 
      GYGAPPRSY GSDGSSQVS GYSSQPPNA GAKHFNDFK 
      EWPSS
    • >TC031430 RB
      MEEYEYGKL FVGGLSWET TQDNLQRFF SRYGEVIDC 
      VVMKNAESG RSRGFGFVT FSDPANVNT VLQNGPHTL 
      DGRTIDPKP CNPRTLQKP KKGGGYPKV FLGGLPSNV 
      TETDLRTFF TRFGKVMEV VIMYDQEKK KSRGFGFLS 
      FEDEESVDR CVSEHFVNL NGKQVEIKK AEPRDGSGG 
      NKMGGADPS SAWGPPQAP MGMMQGPNG QMGGPPMNL 
      GAPMGPNMM QGYQGWGTS PQQQSYASY GTPSGPGSY 
      QGWGAPPAP QGPPPQWGN NYGGPPQQQ GYGSYGPSA 
      GSGTSWNSW NMAQNSGST GPSGYIAGD MYTRGQSGP 
      GGPATPSAP PNMSSSGGS SKPGSDYNY GYGSYASAA 
      DAGGYGAPP RSYGSDGSS QVSGYSSQP PNAGAKHFN 
      DFKEWPSS
    CDS sequence
    • >TC031430 RA
      atggaagaa tacgagtac ggcaaactc tttgtaggg 
      ggcttgtct tgggagaca acccaggac aatcttcaa 
      cgctttttc tctagatat ggcgaagtc atcgattgc 
      gttgttatg aaaaatgca gaatcaggc cgttctaga 
      ggatttggt ttcgttaca ttttcagat ccagccaat 
      gtaaatacg gtacttcaa aatggccca catacacta 
      gacggaaga actatcgac ccgaaacca tgtaatcca 
      agaacttta caaaagccc aaaaagggc ggaggttat 
      ccgaaggta ttcctaggg ggtttacct tcaaacgtg 
      acggaaacc gatctgagg acgttcttc actcggttt 
      ggaaaagtt atggaagtt gttattatg tatgaccag 
      gagaagaaa aaatcaaga ggttttggt ttcttgtcg 
      tttgaagac gaggagtca gtcgaccgc tgcgtctcc 
      gaacacttc gtgaaccta aacggcaaa caagtcgag 
      atcaagaaa gccgagcct cgtgacggc tccggcggt 
      aataaaatg ggcggtgct gacccatcg tcagcttgg 
      ggtccaccg caagcgccc atgggcatg atgcaaggt 
      cccaatggt caaatgggc ggtcctccg atgaatttg 
      ggcgctccg atggggccc aacatgatg cagggttac 
      caaggctgg ggcacatcg ccacagcag caaagttac 
      gccagttat ggtacaccg tcaggtcct ggctcgtac 
      cagggatgg ggtgctccg ccggcgccg caaggaccg 
      cccccacag tggggcaat aactatggg ggaccgccg 
      caacagcaa ggctatggg tcgtacggt ccgagtgct 
      gggagcgga acgagctgg aacagctgg aatatggcg 
      cagaatagc ggatccaca ggttacatt gcaggcgac 
      atgtacacc cgtggtcag tcaggcccc ggcggtccg 
      gccaccccc agtgcgccg ccaaacatg tcatcgtcg 
      ggcggcagc tcgaagccc ggctccgat tacaactac 
      ggctacggt tcgtatgcg tcggcggcg gacgccggc 
      ggctacggc gccccgccg cgctcctac ggcagcgac 
      ggcagcagt caagtgagc ggatactcc tcccagcct 
      ccaaacgca ggtgctaaa catttcaat gattttaaa 
      gagtggccc agttcttga
    • >TC031430 RB
      atggaagaa tacgagtac ggcaaactc tttgtaggg 
      ggcttgtct tgggagaca acccaggac aatcttcaa 
      cgctttttc tctagatat ggcgaagtc atcgattgc 
      gttgttatg aaaaatgca gaatcaggc cgttctaga 
      ggatttggt ttcgttaca ttttcagat ccagccaat 
      gtaaatacg gtacttcaa aatggccca catacacta 
      gacggaaga actatcgac ccgaaacca tgtaatcca 
      agaacttta caaaagccc aaaaagggc ggaggttat 
      ccgaaggta ttcctaggg ggtttacct tcaaacgtg 
      acggaaacc gatctgagg acgttcttc actcggttt 
      ggaaaagtt atggaagtt gttattatg tatgaccag 
      gagaagaaa aaatcaaga ggttttggt ttcttgtcg 
      tttgaagac gaggagtca gtcgaccgc tgcgtctcc 
      gaacacttc gtgaaccta aacggcaaa caagtcgag 
      atcaagaaa gccgagcct cgtgacggc tccggcggt 
      aataaaatg ggcggtgct gacccatcg tcagcttgg 
      ggtccaccg caagcgccc atgggcatg atgcaaggt 
      cccaatggt caaatgggc ggtcctccg atgaatttg 
      ggcgctccg atggggccc aacatgatg cagggttac 
      caaggctgg ggcacatcg ccacagcag caaagttac 
      gccagttat ggtacaccg tcaggtcct ggctcgtac 
      cagggatgg ggtgctccg ccggcgccg caaggaccg 
      cccccacag tggggcaat aactatggg ggaccgccg 
      caacagcaa ggctatggg tcgtacggt ccgagtgct 
      gggagcgga acgagctgg aacagctgg aatatggcg 
      cagaatagc ggatccaca ggtccgtca ggttacatt 
      gcaggcgac atgtacacc cgtggtcag tcaggcccc 
      ggcggtccg gccaccccc agtgcgccg ccaaacatg 
      tcatcgtcg ggcggcagc tcgaagccc ggctccgat 
      tacaactac ggctacggt tcgtatgcg tcggcggcg 
      gacgccggc ggctacggc gccccgccg cgctcctac 
      ggcagcgac ggcagcagt caagtgagc ggatactcc 
      tcccagcct ccaaacgca ggtgctaaa catttcaat 
      gattttaaa gagtggccc agttcttga
    mRNA sequence
    • >TC031430 RA
      tggccagtt tcggtgacg cagtcgttg attccatcc 
      cttccctaa aatggtttc taactcccc taccctgac 
      ggtacgtca tgtctgtgt attcctatc gtagttgtt 
      aattaggat agttcttaa cgttagatt aattaattt 
      taagtttag tgtaagaag tcattattt gtaatggaa 
      gaatacgag tacggcaaa ctctttgta gggggcttg 
      tcttgggag acaacccag gacaatctt caacgcttt 
      ttctctaga tatggcgaa gtcatcgat tgcgttgtt 
      atgaaaaat gcagaatca ggccgttct agaggattt 
      ggtttcgtt acattttca gatccagcc aatgtaaat 
      acggtactt caaaatggc ccacataca ctagacgga 
      agaactatc gacccgaaa ccatgtaat ccaagaact 
      ttacaaaag cccaaaaag ggcggaggt tatccgaag 
      gtattccta gggggttta ccttcaaac gtgacggaa 
      accgatctg aggacgttc ttcactcgg tttggaaaa 
      gttatggaa gttgttatt atgtatgac caggagaag 
      aaaaaatca agaggtttt ggtttcttg tcgtttgaa 
      gacgaggag tcagtcgac cgctgcgtc tccgaacac 
      ttcgtgaac ctaaacggc aaacaagtc gagatcaag 
      aaagccgag cctcgtgac ggctccggc ggtaataaa 
      atgggcggt gctgaccca tcgtcagct tggggtcca 
      ccgcaagcg cccatgggc atgatgcaa ggtcccaat 
      ggtcaaatg ggcggtcct ccgatgaat ttgggcgct 
      ccgatgggg cccaacatg atgcagggt taccaaggc 
      tggggcaca tcgccacag cagcaaagt tacgccagt 
      tatggtaca ccgtcaggt cctggctcg taccaggga 
      tggggtgct ccgccggcg ccgcaagga ccgccccca 
      cagtggggc aataactat gggggaccg ccgcaacag 
      caaggctat gggtcgtac ggtccgagt gctgggagc 
      ggaacgagc tggaacagc tggaatatg gcgcagaat 
      agcggatcc acaggttac attgcaggc gacatgtac 
      acccgtggt cagtcaggc cccggcggt ccggccacc 
      cccagtgcg ccgccaaac atgtcatcg tcgggcggc 
      agctcgaag cccggctcc gattacaac tacggctac 
      ggttcgtat gcgtcggcg gcggacgcc ggcggctac 
      ggcgccccg ccgcgctcc tacggcagc gacggcagc 
      agtcaagtg agcggatac tcctcccag cctccaaac 
      gcaggtgct aaacatttc aatgatttt aaagagtgg 
      cccagttct tgacaacct ttcgataac ggcaacaac 
      agcgcatcg aacgccacc gtctcctcc tcctccacc 
      aataacaac aaaaacgtg cgcttgtac gtgaatgcg 
      gccgtcaaa gccaccttt tacaccgac gacgaggag 
      accgtggcg ttcaagcac tacaacaac ttgagtaat 
      aatttgggt atgtgttgt aacagttgg aatcgtgtt 
      ggcttgatt aacatgaag agagcagcc ggactggtc 
      actcccgcc aaaatcaat tattctgat tagattgac 
      atatgagaa atcgacggg acacataat aacactcac 
      atgcacaca caccgtctg actttcgca gctattttt 
      actttttct tttatttat tgtcaggag agcacgccc 
      tctaacggc agaccgaca accttgcca gaggacgct 
      tccgacaca cactaacta acactacag acagacaga 
      agtgttgac taattttaa gcactgctc aagttgtta 
      ttaatatta attaataaa atagtgtaa attcaattc 
      ttacctcat atctctatt tcaaagtta gagtgtctg 
      cgtggttgc tgtggctaa caaacaaaa accgaaaaa 
      aatcgagaa acgacaatg tgattctta gtttgtagg 
      caaaacttc cctaagtta aatgcattt acaatagtg 
      gtaataacg aacgtattt aatatttat gatgtagtc 
      ctggaacgg atcacttga ttattgtaa gttgaacgt 
      gctctagtc ccatgtctc aatatcaaa tcgtaggtg 
      gcgtccttg tacatatac tatataaat gtattttat 
      ctaagttat gcaaattga ccagcagac aatctatcc 
      ataaggagt aaataattg taagttaag gtcactatt 
      aataatttt atataatag cacacaacc acctagcta 
      agtaggacg taagatctg gctgtacct agtaataga 
      ccttgtttt gttttcttt ttttaaatt atgttacga 
      cttttagtt ttaatacgc gaccgaacg tctggctat 
      aatgagact ttttcaaac atcacatat caaaagttt 
      gcgcttttg aaaaaggtt cgttcactt ataacggac 
      gtttccgac tcgaatcat gtacatttt atacttttt 
      ttttttttg taaacctat ttattctaa ttttagttc 
      gattaaaat tgagttaca ccgaaactt tgaattagg 
      ggttgtcag gggggaggg gcgcgattc ggattaaat 
      aagctataa ctaatagat tagcagtta acgaattca 
      tggtgattt atatttagt agaagtatt ctgtctgtc 
      tgggcctca tgtgtctat ctaatcaag cgagtaatt 
      ggttcgttt gttttaagt gatcttctt attttttat 
      ttaaaaatt gagtgtgac aatctacat taagcaatg 
      tttgatagc aacctagac gtaactgat attgttttc 
      tagttttaa ttgtaaaat attcctcgg ttaacgcag 
      accaataca attttagaa gaaaatcaa gttgaaaca 
      aaaataaag cgctctttt taaa
    • >TC031430 RB
      tggccagtt tcggtgacg cagtcgttg attccatcc 
      cttccctaa aatggtttc taactcccc taccctgac 
      ggtacgtca tgtctgtgt attcctatc gtagttgtt 
      aattaggat agttcttaa cgttagatt aattaattt 
      taagtttag tgtaagaag tcattattt gtaatggaa 
      gaatacgag tacggcaaa ctctttgta gggggcttg 
      tcttgggag acaacccag gacaatctt caacgcttt 
      ttctctaga tatggcgaa gtcatcgat tgcgttgtt 
      atgaaaaat gcagaatca ggccgttct agaggattt 
      ggtttcgtt acattttca gatccagcc aatgtaaat 
      acggtactt caaaatggc ccacataca ctagacgga 
      agaactatc gacccgaaa ccatgtaat ccaagaact 
      ttacaaaag cccaaaaag ggcggaggt tatccgaag 
      gtattccta gggggttta ccttcaaac gtgacggaa 
      accgatctg aggacgttc ttcactcgg tttggaaaa 
      gttatggaa gttgttatt atgtatgac caggagaag 
      aaaaaatca agaggtttt ggtttcttg tcgtttgaa 
      gacgaggag tcagtcgac cgctgcgtc tccgaacac 
      ttcgtgaac ctaaacggc aaacaagtc gagatcaag 
      aaagccgag cctcgtgac ggctccggc ggtaataaa 
      atgggcggt gctgaccca tcgtcagct tggggtcca 
      ccgcaagcg cccatgggc atgatgcaa ggtcccaat 
      ggtcaaatg ggcggtcct ccgatgaat ttgggcgct 
      ccgatgggg cccaacatg atgcagggt taccaaggc 
      tggggcaca tcgccacag cagcaaagt tacgccagt 
      tatggtaca ccgtcaggt cctggctcg taccaggga 
      tggggtgct ccgccggcg ccgcaagga ccgccccca 
      cagtggggc aataactat gggggaccg ccgcaacag 
      caaggctat gggtcgtac ggtccgagt gctgggagc 
      ggaacgagc tggaacagc tggaatatg gcgcagaat 
      agcggatcc acaggtccg tcaggttac attgcaggc 
      gacatgtac acccgtggt cagtcaggc cccggcggt 
      ccggccacc cccagtgcg ccgccaaac atgtcatcg 
      tcgggcggc agctcgaag cccggctcc gattacaac 
      tacggctac ggttcgtat gcgtcggcg gcggacgcc 
      ggcggctac ggcgccccg ccgcgctcc tacggcagc 
      gacggcagc agtcaagtg agcggatac tcctcccag 
      cctccaaac gcaggtgct aaacatttc aatgatttt 
      aaagagtgg cccagttct tgacaacct ttcgataac 
      ggcaacaac agcgcatcg aacgccacc gtctcctcc 
      tcctccacc aataacaac aaaaacgtg cgcttgtac 
      gtgaatgcg gccgtcaaa gccaccttt tacaccgac 
      gacgaggag accgtggcg ttcaagcac tacaacaac 
      ttgagtaat aatttgggt atgtgttgt aacagttgg 
      aatcgtgtt ggcttgatt aacatgaag agagcagcc 
      ggactggtc actcccgcc aaaatcaat tattctgat 
      tagattgac atatgagaa atcgacggg acacataat 
      aacactcac atgcacaca caccgtctg actttcgca 
      gctattttt actttttct tttatttat tgtcaggag 
      agcacgccc tctaacggc agaccgaca accttgcca 
      gaggacgct tccgacaca cactaacta acactacag 
      acagacaga agtgttgac taattttaa gcactgctc 
      aagttgtta ttaatatta attaataaa atagtgtaa 
      attcaattc ttacctcat atctctatt tcaaagtta 
      gagtgtctg cgtggttgc tgtggctaa caaacaaaa 
      accgaaaaa aatcgagaa acgacaatg tgattctta 
      gtttgtagg caaaacttc cctaagtta aatgcattt 
      acaatagtg gtaataacg aacgtattt aatatttat 
      gatgtagtc ctggaacgg atcacttga ttattgtaa 
      gttgaacgt gctctagtc ccatgtctc aatatcaaa 
      tcgtaggtg gcgtccttg tacatatac tatataaat 
      gtattttat ctaagttat gcaaattga ccagcagac 
      aatctatcc ataaggagt aaataattg taagttaag 
      gtcactatt aataatttt atataatag cacacaacc 
      acctagcta agtaggacg taagatctg gctgtacct 
      agtaataga ccttgtttt gttttcttt ttttaaatt 
      atgttacga cttttagtt ttaatacgc gaccgaacg 
      tctggctat aatgagact ttttcaaac atcacatat 
      caaaagttt gcgcttttg aaaaaggtt cgttcactt 
      ataacggac gtttccgac tcgaatcat gtacatttt 
      atacttttt ttttttttg taaacctat ttattctaa 
      ttttagttc gattaaaat tgagttaca ccgaaactt 
      tgaattagg ggttgtcag gggggaggg gcgcgattc 
      ggattaaat aagctataa ctaatagat tagcagtta 
      acgaattca tggtgattt atatttagt agaagtatt 
      ctgtctgtc tgggcctca tgtgtctat ctaatcaag 
      cgagtaatt ggttcgttt gttttaagt gatcttctt 
      attttttat ttaaaaatt gagtgtgac aatctacat 
      taagcaatg tttgatagc aacctagac gtaactgat 
      attgttttc tagttttaa ttgtaaaat attcctcgg 
      ttaacgcag accaataca attttagaa gaaaatcaa 
      gttgaaaca aaaataaag cgctctttt taaa