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Gene information
General information
TC016198 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4564154..4566408
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016198
    iBeetle screen iB_02555

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head sometimes partiallyremaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus sometimes remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva dorsal not closed - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: thoracic legs seem to be more affected than head appendages
    larva appendages irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016198 RA
      MSENNADFL NNYWKTVNS GTGNPSASV QRKCMAQIY 
      NHSLPTLDT ATICHVLEK RLHKYSDLV DNDNKNSDR 
      IKKVIQQPV NLQSNLSNV DFKSLLKPV LGPKSDNLP 
      DFDNFKAAF HKKPQKPSF ENRKIIKPF APQQLSRTC 
      SEELPKTTS FKTASNEFQ LQNAKKWGT GAQKPRLGL 
      SRNVNSKFV PPLRSHQPP EEPKPEQID DRLKNIDPK 
      MVELIKSEI MDVGAKVEW GDIAGLEFA KTAIQEAVV 
      WPMLRPDIF TGLRRPPKG ILLFGPPGT GKTLIGKCV 
      AAQSKSTFF SISASSLTS KWIGDGEKM VRALFAVAR 
      CHQPAVIFI DEIDSLLCQ RNETEHESS RRIKTEFLV 
      QLDGATTDS EERLLVIGA TNRPQELDE AARRRFVKR 
      LYIPLPEYE ARLQLVTGL IANERHDLD SDDLAKVAQ 
      LSEGYSGAD IRSLCSEAS LGPIRSIDM SMIAKIQAH 
      EVRPLTMDD FHKAFTRVR SSVSPKDLE QYVIWDKTY 
      GSGL
    CDS sequence
    • >TC016198 RA
      atgagtgaa aataatgcc gatttttta aacaattat 
      tggaaaaca gttaacagt ggaacgggg aatccaagt 
      gcgtcagtt caaagaaaa tgtatggcg caaatttac 
      aaccattca ttaccgaca ttggataca gcaactatt 
      tgtcacgta ttggaaaaa cgtttgcac aaatatagt 
      gaccttgtc gataacgac aataaaaat agtgatcga 
      attaagaaa gtaatacaa caaccggtt aatctgcaa 
      agtaattta tcaaatgtt gactttaag tcattacta 
      aaaccagta cttggccct aaatcggac aacctcccc 
      gattttgac aatttcaaa gcggctttt cacaaaaag 
      ccccaaaaa ccgagcttc gaaaaccga aaaatcatc 
      aaacccttc gccccccaa caactctcg cgcacatgt 
      tcggaagaa ctgccaaaa accacgtcc tttaagacg 
      gccagtaac gaattccag ctccaaaac gccaaaaaa 
      tggggcacc ggggcccaa aaacccagg ctaggactt 
      agccgtaac gtcaatagc aaattcgtg cccccttta 
      cgatcacat caacccccc gaagagccc aaacccgaa 
      caaatcgac gaccgtttg aaaaacatc gacccgaaa 
      atggtcgaa ctgatcaaa tccgaaatc atggacgtg 
      ggggccaaa gtggaatgg ggcgacatc gcaggcctg 
      gaattcgcc aaaaccgcc atccaagag gccgtggtg 
      tggcccatg ctgcggccc gatattttc acggggctg 
      aggcgcccc ccgaagggc atcctcctg ttcgggccg 
      cccggcact ggaaagaca ctgatcggg aaatgtgtg 
      gcggcgcaa agcaagtcg acttttttc agcatcagt 
      gcctcctct ctgacttcg aaatggatc ggtgatggg 
      gagaaaatg gtgagggcg ctgtttgcg gtcgccagg 
      tgccaccaa cccgctgtt atttttatc gatgagatt 
      gattcgttg ttgtgccaa cgcaatgaa actgaacat 
      gagagttca cggagaatt aaaaccgag tttttggtg 
      cagttagat ggcgccact acggatagt gaggaacgg 
      ttgttggtg attggggcc accaatcgc ccccaggag 
      ctggacgag gcggcgagg aggaggttt gtgaagagg 
      ctgtacata cccttgccc gaatatgag gctcgttta 
      cagttggtg actggatta attgcgaat gaaagacac 
      gatttggat agtgatgac cttgcaaaa gtggcccaa 
      ttatcagag gggtactca ggggctgat ataagaagt 
      ttgtgctcc gaggcgtcc ctggggccc attaggtcg 
      atcgacatg agtatgatt gcgaaaatt caagcgcat 
      gaggtacgg ccgttgaca atggacgat ttccacaaa 
      gctttcacc agagttagg tcgagtgtt tcccctaag 
      gatttggaa caatacgtt atttgggat aaaacttat 
      ggctcagga ttataa
    mRNA sequence
    • >TC016198 RA
      cctaaccaa gaattttga aagtgtttg aggttaggt 
      ttgcaaatt ttccaaaaa tgagtgaaa ataatgccg 
      attttttaa acaattatt ggaaaacag ttaacagtg 
      gaacgggga atccaagtg cgtcagttc aaagaaaat 
      gtatggcgc aaatttaca accattcat taccgacat 
      tggatacag caactattt gtcacgtat tggaaaaac 
      gtttgcaca aatatagtg accttgtcg ataacgaca 
      ataaaaata gtgatcgaa ttaagaaag taatacaac 
      aaccggtta atctgcaaa gtaatttat caaatgttg 
      actttaagt cattactaa aaccagtac ttggcccta 
      aatcggaca acctccccg attttgaca atttcaaag 
      cggcttttc acaaaaagc cccaaaaac cgagcttcg 
      aaaaccgaa aaatcatca aacccttcg ccccccaac 
      aactctcgc gcacatgtt cggaagaac tgccaaaaa 
      ccacgtcct ttaagacgg ccagtaacg aattccagc 
      tccaaaacg ccaaaaaat ggggcaccg gggcccaaa 
      aacccaggc taggactta gccgtaacg tcaatagca 
      aattcgtgc cccctttac gatcacatc aaccccccg 
      aagagccca aacccgaac aaatcgacg accgtttga 
      aaaacatcg acccgaaaa tggtcgaac tgatcaaat 
      ccgaaatca tggacgtgg gggccaaag tggaatggg 
      gcgacatcg caggcctgg aattcgcca aaaccgcca 
      tccaagagg ccgtggtgt ggcccatgc tgcggcccg 
      atattttca cggggctga ggcgccccc cgaagggca 
      tcctcctgt tcgggccgc ccggcactg gaaagacac 
      tgatcggga aatgtgtgg cggcgcaaa gcaagtcga 
      cttttttca gcatcagtg cctcctctc tgacttcga 
      aatggatcg gtgatgggg agaaaatgg tgagggcgc 
      tgtttgcgg tcgccaggt gccaccaac ccgctgtta 
      tttttatcg atgagattg attcgttgt tgtgccaac 
      gcaatgaaa ctgaacatg agagttcac ggagaatta 
      aaaccgagt ttttggtgc agttagatg gcgccacta 
      cggatagtg aggaacggt tgttggtga ttggggcca 
      ccaatcgcc cccaggagc tggacgagg cggcgagga 
      ggaggtttg tgaagaggc tgtacatac ccttgcccg 
      aatatgagg ctcgtttac agttggtga ctggattaa 
      ttgcgaatg aaagacacg atttggata gtgatgacc 
      ttgcaaaag tggcccaat tatcagagg ggtactcag 
      gggctgata taagaagtt tgtgctccg aggcgtccc 
      tggggccca ttaggtcga tcgacatga gtatgattg 
      cgaaaattc aagcgcatg aggtacggc cgttgacaa 
      tggacgatt tccacaaag ctttcacca gagttaggt 
      cgagtgttt cccctaagg atttggaac aatacgtta 
      tttgggata aaacttatg gctcaggat tataaagtg 
      aaggatgta attatgtga tttaataaa gtaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY