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Gene information
General information
TC016197 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 4566489..4568578
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016197
    iBeetle screen iB_02554

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016197 RA
      MLKSSAENK ILKLLPNDK PITAIQVIE NLDKCPKGF 
      HPISRTYDQ DQDADLRES SIFKSSAAR YLCVSKSEG 
      LPNFVIQEL FVLTEKFNP PRGFSLLNR TADSEQKAW 
      KKKQLCYKL VNLRDTKVA VTDIIVCSR LKKAPEGFK 
      LAGELNGVT VCFKMGNVQ DTQNSPDSN GRNITPPVR 
      PAPPYPGNG PIYPSIGAD GGDHDYEIL KPPGYPSGP 
      SRPAPRPPA PNPRQNSTT GTLGASGGN NLDGVPFIV 
      NPKFLNAST ADRFQFPTI RAKTMQQIL KEYDYAFTV 
      ERQT
    CDS sequence
    • >TC016197 RA
      atgttaaaa tcgtctgcc gaaaataaa atactgaag 
      ttactaccg aacgataaa cccatcaca gcaatccaa 
      gtcattgaa aaccttgac aaatgcccc aaagggttt 
      cacccaatc agtaggacc tacgaccaa gaccaggat 
      gctgatctg agggaaagc tcaattttt aaaagtagt 
      gcggctaga tatttgtgt gtttcgaaa tctgaaggt 
      ctccccaat tttgtgatt caggagctt tttgtactg 
      actgagaag tttaatcct ccgagaggt tttagtttg 
      ttgaatagg actgccgat agtgaacag aaggcttgg 
      aagaaaaaa cagttgtgt tataaatta gtgaatcta 
      agggatacg aaagttgca gttactgat attatcgtc 
      tgtagtcgg ctgaagaaa gctcctgag gggtttaaa 
      cttgctggg gaattgaac ggtgtgacg gtttgcttc 
      aaaatgggc aacgtgcaa gacacccaa aacagcccc 
      gattccaac ggtcgcaac atcactccc cctgtccgc 
      ccagcgccc ccatatccc ggtaacgga cccatttac 
      ccatcgatt ggtgccgat ggcggcgac cacgactac 
      gaaattcta aaacctcca ggttatccc tcgggacca 
      tcacgaccc gctcccaga ccaccagct ccaaaccct 
      cgtcaaaac tcaaccaca gggacgttg ggtgcaagt 
      ggagggaat aatctagat ggtgtaccg tttattgtc 
      aacccgaaa tttttgaat gcaagcact gccgatagg 
      tttcagttt cccacaatt agggccaag acaatgcaa 
      caaattttg aaggagtat gattatgct tttaccgtg 
      gaacggcaa acttaa
    mRNA sequence
    • >TC016197 RA
      aggtaaaat agtggacaa agcgcctga aattgcgtc 
      atatttgcg atttactga gtaataatt tattaatag 
      tttgcataa atattaatt tgtggctat ttttacaca 
      atggaagag atacgtcca aagactgac tacgatgtt 
      aaaatcgtc tgccgaaaa taaaatact gaagttact 
      accgaacga taaacccat cacagcaat ccaagtcat 
      tgaaaacct tgacaaatg ccccaaagg gtttcaccc 
      aatcagtag gacctacga ccaagacca ggatgctga 
      tctgaggga aagctcaat ttttaaaag tagtgcggc 
      tagatattt gtgtgtttc gaaatctga aggtctccc 
      caattttgt gattcagga gctttttgt actgactga 
      gaagtttaa tcctccgag aggttttag tttgttgaa 
      taggactgc cgatagtga acagaaggc ttggaagaa 
      aaaacagtt gtgttataa attagtgaa tctaaggga 
      tacgaaagt tgcagttac tgatattat cgtctgtag 
      tcggctgaa gaaagctcc tgaggggtt taaacttgc 
      tggggaatt gaacggtgt gacggtttg cttcaaaat 
      gggcaacgt gcaagacac ccaaaacag ccccgattc 
      caacggtcg caacatcac tccccctgt ccgcccagc 
      gcccccata tcccggtaa cggacccat ttacccatc 
      gattggtgc cgatggcgg cgaccacga ctacgaaat 
      tctaaaacc tccaggtta tccctcggg accatcacg 
      acccgctcc cagaccacc agctccaaa ccctcgtca 
      aaactcaac cacagggac gttgggtgc aagtggagg 
      gaataatct agatggtgt accgtttat tgtcaaccc 
      gaaattttt gaatgcaag cactgccga taggtttca 
      gtttcccac aattagggc caagacaat gcaacaaat 
      tttgaagga gtatgatta tgcttttac cgtggaacg 
      gcaaactta aaaatgtta tttttttaa taaacggaa 
      ctgagatct tttaggaat gttttaatg cctctatct 
      agttatatt ttagagact ctatcatca tttgtgtta 
      cactgtatt attttttag caatcaagt gtgtcatta 
      ttgtagtca cggtaggct caagtgatt caagttaag 
      tcatgtaag aaatttaac gaagagttg tatagtttg 
      taaacttaa gtgcccact agattgtat caacacata 
      ttatgatta taacaatta ttatttagt gccaatgtt 
      ttatattta atacagtat ttatgtaca g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY