Status
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Gene information
General information
TC031972
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016163
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid3: 5618..35049
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031972
    iBeetle screen iB_02553
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031972 RA
      MADAAARQL QYEYKANSN LVLQADVRL IERRSRDEA 
      TGEVVSLVG KLSGTKMGD RAQRTRPGK AEERKVKRQ 
      KRDEAQYDF ARMKGATLL SEGVDEMVG IIYRPKTQE 
      TRQTYEVLL SFLQEALGD QPRDILCGA ADEVLTVLK 
      NDRLKEKEK KKETEMLLG SIAEERFAL LVNLGKKIT 
      DFGNEENKI SMNEENIDE TYGINVQFE ESEEEDDED 
      MYGEVREDM DDEEGEEAK EDSAIHAEN LGGVEEMKK 
      EKALHPLDI DAYWLQRRL SKIYDDAMV SQAKAAEVL 
      NVLRDAGDD RECENQLVL LLGYDCFDF IKQLKKHRQ 
      MILYCTLLA KSQSESERQ KIKDKMSED PSLTRILRL 
      LETGKGDDD GEDEESTSR SSSRKDQSD EMDTGSGAQ 
      VAGSRHLLD LEDLVFTQG SHYMANKRC QLPDGSFRK 
      QRKGYEEVH VPALKPKPF GDNEKLQSI DQLPKYVQP 
      VFEGFKTLN RIQSRLYKT ALESDENLL LCAPTGAGK 
      TNVALLCMM REIGKHINT DGTINADDF KIIYVAPMR 
      SLVQEMVGN FGKRLSSYN ITVSELTGD HQLTREQIA 
      GTQIIVCTP EKWDIITRK GGEKTFTSL VRLIIIDEI 
      HLLHDERGP VLEALVART IRMIESTQE DVRLVGLSA 
      TLPNYQDVA AFLRVKSDT GLFYFDNSF RPVALEQQY 
      IGVTEKKAL KRYQVMNEI VYEKTMEHA GRNQVLIFV 
      HSRKETGKT ARAIRDMCL EKDTLGQFL KEGSASMEV 
      LRTEAEQVK NHELKDLLP YGFAIHHAG MTRVDRTLV 
      EDLFADRHI QVLVSTATL AWGVNLPAH TVIIKGTQI 
      YNPEKGRWV ELGALDVLQ MLGRAGRPQ YDTRGEGIL 
      ITNHSELQY YLSLLNQQL PIESQMISK LSDMLNAEI 
      VLGTVQNVR DAVTWLGYT YLYIRMLRQ PTLYGVSHD 
      HFKQDPLLE QHRADLIHT AALYLDRSG LVKYDRKTG 
      QFQVTELGR ISSHYYCTH ETMQTYNQL LKPMLSEIE 
      LFRVFSLSS EFRNITVRE EEKLELQKL MERVPIPIK 
      ESIEEPSAK VNVLLQAYI SQLKLEGFA LMSDMVYVT 
      QSASRLMRA IFEIVLHRG WAQLADKSL ALCKMIDKR 
      MWQSMSPLR QFRKMPEEI VKKIEKKNF PWERLYDLG 
      PNEIGELIR VPKLGKTIH KYVHQFPKL ELSTHIQPI 
      TRSMLKVEL TITPDFQWD EKLHGASEA FWILVEDVD 
      SEVILHHEY FLLKAKYSQ DEHLVKFFV PIFEPLPPQ 
      YFLRIVSDR WIGAETQLP VSFRHLILP EKNFPPTEL 
      LDLQPLPVT ALRNDKFES LYNDKFPQF NPIQTQVFN 
      SVYNGDDNI FIGAPTGSG KTTIAEFAI LRLFDKNPE 
      GRCVYLVPK DALAELTFA DWHNKFGQI LGKKVVLLT 
      GETGTDLKL LAKGQIVIS TAEKWDVLS RRWKQRKNV 
      QNVNLFIVD ELHLIGGED GPVIEVVCS RMRYISSQI 
      EKPIRIIAL SASLMDYRD VSQWLGCNA NATFNFHPS 
      VRPVPLELH VQGINITHN ASRLIAMAK PVYNAVVRY 
      SPHKPVIVF VPTRKQSRL TAIDLLTYA ASEGQSNKF 
      FHAEEEDIK PFLDRMTDK TLKETLSQG VAYMHEGLT 
      ASDLRLVEQ LFDSGAVQI AVVSKDLCW TVNIYSYLV 
      IIMDTQFYN GKVHSYEDY PITDVLQMV GRANRPLED 
      DDAKCVLMC QSTKKDFFK KFLSEPLPV ESHLDHRLH 
      DHFNAEIVT KTIENKQDA VDYLTWTFL YRRLTQNPN 
      YYNLQGVTH RHLSDHLSE LVENTLSDL EQSKCISVE 
      EDMDCVPLN LGMIAAYYY INYTTIELF SLSLNSKTK 
      IRGLLEIIS SAAEYEDLS VRHREDNIL RQLATKLPN 
      KLTSSSGSQ PKYNDPHVK TNLLIQAHL CRLQLGAEL 
      QGDTEIVLS KAIRLIQAC VDVLSSNGW LSPAVAAME 
      LAQMVTQAM WSKDSYLKQ LPHFTTEII KRCTDKGVE 
      TVFDIMELE DEDRSKLLQ LSDSQMADV ARFCNRYPN 
      IELSYEVLD KDKIHSGSS VHVAVQLER EDEVNGPVI 
      APFFPQKRE EGWWVVIGD PKTNSLLSI KRLTLQQKA 
      RVKLDFVAP SPGHHNYTL YFMSDAYLG CDQEYKFSI 
      DVGDFESSE SESE
    CDS sequence
    • >TC031972 RA
      atggcggat gctgcggca aggcagctt caatacgaa 
      tataaagct aactcaaat ctcgtgctt caagctgat 
      gttcgtctg attgagaga agaagtcga gatgaggct 
      acaggtgaa gttgtttcc cttgttgga aaactatcg 
      gggactaaa atgggtgac agggcacag aggactcga 
      cccggcaaa gcagaagaa cggaaagtt aagaggcaa 
      aaacgtgac gaagcccaa tatgatttc gcccgaatg 
      aagggggcc acattactt tcagaaggt gtggatgaa 
      atggtggga ataatatat cgacctaaa acgcaagaa 
      actagacaa acgtatgaa gtgcttttg agttttttg 
      caagaagca ttgggggat caaccaaga gatattctt 
      tgtggtgca gcagatgag gtcttaaca gtattaaag 
      aacgacagg ttgaaggag aaagaaaag aaaaaggag 
      actgaaatg ctgttaggc tctattgct gaggagaga 
      tttgcttta ctcgttaat ctgggtaaa aaaattact 
      gattttgga aatgaagaa aataaaata tctatgaat 
      gaagaaaac attgatgaa acttatgga attaatgtt 
      cagtttgaa gaatcagag gaggaagat gatgaagac 
      atgtatgga gaagttcgc gaggatatg gatgatgaa 
      gaaggagaa gaagctaaa gaggatagt gctatacat 
      gcagagaat cttggtggt gttgaagaa atgaaaaag 
      gaaaaagca cttcatcct cttgatata gacgcttat 
      tggctgcaa aggcgcctt tcaaaaatt tatgacgat 
      gcaatggtt tcgcaggca aaagcggcg gaagtgtta 
      aatgttctt cgcgatgct ggagatgat cgagagtgc 
      gaaaatcaa cttgtttta ctactaggt tacgattgc 
      tttgacttt attaaacag ttgaaaaaa cataggcag 
      atgatttta tattgcaca ttattggcc aaatcacag 
      agtgaatca gaaagacag aaaatcaaa gataaaatg 
      tctgaagat cccagtctt actcgtatt ttgcgtctt 
      ctggaaacc ggaaaaggg gatgatgat ggtgaagac 
      gaagaatca acttcaaga tcatcaagc cgaaaagat 
      caatcggat gagatggat acaggtagt ggagcacaa 
      gttgctgga tcaagacac ctacttgac ttggaagat 
      ttggtgttt acgcaggga tcgcattac atggcaaat 
      aaacgctgt cagttaccg gatggatct ttcaggaag 
      cagaggaaa ggatacgaa gaggtacac gttcctgct 
      ctaaaacct aaaccgttt ggagataac gaaaagtta 
      caatcaatt gatcagtta cccaaatac gttcagcct 
      gtttttgaa ggcttcaaa acactaaat cgtattcag 
      agtcgctta tataaaaca gcactcgaa tcagatgag 
      aatttactc ttgtgtgca cctacaggt gccggtaaa 
      accaacgta gcactattg tgtatgatg cgtgaaata 
      ggcaaacac atcaacaca gatggtaca ataaacgcc 
      gatgacttt aagatcata tacgttgca ccaatgcgt 
      tctctcgta caagaaatg gtcggtaat tttgggaaa 
      agattatcg agttacaat atcactgta tcagaattg 
      actggtgat catcagtta actagagaa cagatcgct 
      gggacacaa atcatcgta tgcacccct gaaaagtgg 
      gatattata acacgaaaa ggaggcgaa aaaacattc 
      acttcgttg gtaagacta attattatc gacgaaatt 
      catttgtta cacgatgag agaggtccg gttctggaa 
      gctttggtt gctcgaaca attcgcatg attgaatcg 
      actcaagaa gacgtacgt ctggttggt ctttcggct 
      actttacct aattaccaa gatgttgcc gcgtttctt 
      cgagtcaaa tcagatact ggtcttttt tatttcgat 
      aatagtttt cgaccagtt gctttggaa caacaatat 
      atcggagtg acggagaaa aaggctttg aaacggtac 
      caagtaatg aatgagatt gtttatgaa aaaaccatg 
      gaacatgca ggacgcaac caagtgtta attttcgtt 
      cattcgaga aaggagacg ggaaaaaca gcgcgtgcg 
      atacgtgat atgtgtttg gagaaggat actttagga 
      cagtttttg aaagaggga tcggcttca atggaagtt 
      ttgcgaacc gaagcggaa caagtgaaa aatcatgaa 
      ttgaaggat ttgttaccg tatggattt gcgatacat 
      catgccgga atgacgagg gttgacagg acattggtg 
      gaagatttg tttgcagat aggcatata caggtgctg 
      gtgtcaact gcaacactt gcctggggc gtaaactta 
      ccagcccac acagtcata ataaaagga acccaaatt 
      tacaatcca gaaaaaggc agatgggtt gaattgggt 
      gctttggac gttcttcaa atgttaggt cgtgccggc 
      cgacctcaa tacgatacc agaggtgaa ggcatattg 
      ataaccaat cacagcgaa ctgcaatac tatctatca 
      ctattgaac caacaatta cccatcgaa tctcaaatg 
      atatcaaaa ttatcagac atgttaaac gctgagatt 
      gttttggga accgtacaa aatgtacga gatgccgtc 
      acatggtta ggttacacg tatttatac attcgaatg 
      cttcgacaa ccaactttg tacggtgtt tcacatgat 
      catttcaaa caagatccg ttattggaa caacatcga 
      gctgattta atacataca gctgcattg tatctcgat 
      cgtagtggt ttggttaaa tatgacaga aaaaccggt 
      cagtttcaa gtaaccgaa ctgggacgc atttcctcg 
      cattattat tgcacccac gaaacgatg caaacttac 
      aatcaattg ctcaaacca atgttgagc gaaattgaa 
      ttgttccgc gttttttcc ctatcgtct gagttcagg 
      aatataaca gtccgtgag gaagagaaa cttgagttg 
      caaaaattg atggaacgt gttccaatc ccgatcaaa 
      gaaagtatc gaagaacca agtgccaaa gtaaacgta 
      ctgttacaa gcgtacatt tctcaacta aaactggaa 
      ggtttcgca cttatgtcc gacatggtt tacgtaaca 
      caatcagct tcgcgttta atgcgtgct atttttgag 
      attgtttta cacagaggt tgggcacaa ttggccgat 
      aaatcgtta gctttgtgt aaaatgatt gataaaaga 
      atgtggcag tcgatgtca ccgttgcgc caatttcgc 
      aaaatgccg gaagagatt gtgaagaaa attgaaaag 
      aaaaatttc ccatgggaa cgtttatac gatttggga 
      ccgaacgaa atcggcgaa ttgattcgg gtcccaaag 
      ttgggcaag actatccat aaatacgtt catcagttc 
      cccaaactt gaattatca acgcatatt caaccaatt 
      accaggtca atgttgaag gttgaattg accataacg 
      cctgacttt cagtgggat gagaaactt catggagcg 
      tctgaagcg ttttggatt ttggtggaa gatgtggat 
      tcagaggtt attctccac catgagtat tttttgctc 
      aaagcgaag tattcacaa gacgagcat ttggttaaa 
      ttttttgtc ccgattttc gaacctttg ccacctcag 
      tacttctta cgtattgtg tcagatcga tggatcgga 
      gccgagaca caattacca gtttctttc cgacatttg 
      atactacca gagaagaat ttcccacca actgaacta 
      cttgacttg caaccttta ccagtcact gccttacgg 
      aacgataaa ttcgagtcg ttgtataac gacaagttc 
      ccgcagttt aatccaatt cagacgcaa gtcttcaat 
      tcggtttac aacggtgat gataatatt tttattggt 
      gccccgacc ggttctgga aaaactaca atcgctgaa 
      tttgcgata cttcgcctt tttgataaa aacccggaa 
      ggtcggtgc gtttatctc gttccgaaa gatgcatta 
      gctgagtta acgtttgcc gattggcac aataaattt 
      gggcaaatt ttgggcaag aaagtagtg ctcctaact 
      ggtgaaacc gggactgat ttgaagtta ctggcaaaa 
      ggtcagatt gttataagc acggccgaa aaatgggat 
      gtattgtcg cgaagatgg aaacaaaga aagaatgtc 
      caaaatgtg aatttgttt atcgttgat gagttgcat 
      ttgattggt ggggaggat gggcctgtt attgaagtt 
      gtgtgttcc cgcatgcga tatatttcc tcacagatt 
      gagaaaccg atcaggatt atagcatta agtgcgtcg 
      cttatggat tatcgagat gtgtctcaa tggttgggt 
      tgtaatgcc aatgcaacg tttaatttt catccgagt 
      gtgaggcct gtaccgttg gaattgcat gtacagggg 
      attaatatt acacataat gcgtcgaga cttatcgca 
      atggcgaag ccggtttat aatgctgtg gtgaggtat 
      agtccacat aagccggtt attgttttc gtaccgacg 
      aggaaacaa tcacgtttg actgcaatt gatttactt 
      acgtatgct gcgtctgag ggacagtct aataagttt 
      ttccatgcg gaggaagag gatattaaa ccgttcttg 
      gatcggatg actgataaa actttgaag gaaacgctt 
      tcccaaggc gttgcatac atgcatgaa ggtttgaca 
      gcaagtgat ttacgccta gttgaacag ttgtttgac 
      tcgggggcg gtacaaatt gctgttgtt agtaaggat 
      ttgtgttgg accgtgaac atttattct tacttggtt 
      atcataatg gacacacaa ttttacaat ggaaaagtt 
      cattcctac gaagattat cccattacc gatgtgtta 
      caaatggtg ggacgtgct aatcgtcca ttggaggat 
      gatgatgcg aaatgtgta ctaatgtgt caaagtacc 
      aagaaggat ttcttcaag aaattccta agtgagccg 
      ttaccggtt gaaagtcat ttggatcat agattacat 
      gaccatttc aatgccgaa atcgtaaca aaaactatc 
      gaaaataaa caggacgct gttgattat ttgacttgg 
      acatttctg tatcgaaga cttacacaa aatccaaac 
      tattacaat ttgcaagga gttacgcac agacatctt 
      tctgaccat ttgtcagag ttggtggaa aatactttg 
      tccgatttg gaacagtca aaatgtatc agcgtggaa 
      gaggacatg gattgtgtg ccacttaat ttgggaatg 
      attgcggca tattattac atcaattac acaactatt 
      gaactattc agtttgtca ctgaacagc aagaccaaa 
      attcgtggt ttattagaa atcatatcg tcggcagcc 
      gaatatgaa gatctttcc gtacgtcat cgcgaggat 
      aacattctt cgccaattg gcaacaaaa ttaccaaat 
      aaactgaca tcttccagt ggaagtcaa ccgaaatat 
      aacgaccca cacgtcaaa acaaatctt ctcattcaa 
      gcacatttg tgtcgactt cagcttggt gctgagctt 
      caaggcgat acagaaatt gtactaagc aaagcaatt 
      cgacttatt caagcttgt gttgatgta cttagttca 
      aatggatgg ctttcgcca gctgtggcc gctatggaa 
      ttggctcaa atggtgaca caagcaatg tggagtaaa 
      gattcgtat ttgaagcaa ttgcctcac tttacgaca 
      gaaattatt aagaggtgt acggataaa ggagtcgaa 
      accgtgttt gatataatg gaattggaa gatgaagac 
      aggtcaaag ctgttgcag ttgagcgat agtcaaatg 
      gctgatgtt gccagattt tgtaatcgg tatccaaat 
      attgaatta agttacgaa gttttggat aaagataag 
      atacattcc gggtcttca gtgcatgta gctgtgcaa 
      cttgagagg gaggatgaa gtgaatggg cctgtcatt 
      gcgcccttt ttcccacag aaacgtgaa gaaggatgg 
      tgggttgtt attggtgat ccgaaaacc aattccttg 
      ctctccatt aaacgcctt actcttcaa caaaaagct 
      agagtcaag ttggatttt gttgcccct agtccagga 
      caccataat tacacttta tattttatg agtgatgcg 
      tatttgggt tgcgatcaa gaatataaa ttttcaata 
      gatgttggt gattttgaa agcagtgaa agtgagtca 
      gagtga
    mRNA sequence
    • >TC031972 RA
      caatttttg taaaatgac aggaaagtg taaaccctg 
      gaatagttt ccctgtaaa tgtttctta aaatcgcca 
      caagagagc tgtgacata cagaatttt gtggaactt 
      tggttaaaa aacagacat ttgacacat gcgacgtgc 
      cgtgtaaga gacgtaaga aagtctttt ttatcattt 
      atcaatcta ggaggccgt gatgcaccc aaacaacct 
      tttttctgc aagcaacct tcaaagtga actagaact 
      tttgctcac gcacagttg gtgaccttg cggccagtg 
      tagccacaa gtgcatcac aagtggata gtttaacaa 
      catggcgga tgctgcggc aaggcagct tcaatacga 
      atataaagc taactcaaa tctcgtgct tcaagctga 
      tgttcgtct gattgagag aagaagtcg agatgaggc 
      tacaggtga agttgtttc ccttgttgg aaaactatc 
      ggggactaa aatgggtga cagggcaca gaggactcg 
      acccggcaa agcagaaga acggaaagt taagaggca 
      aaaacgtga cgaagccca atatgattt cgcccgaat 
      gaagggggc cacattact ttcagaagg tgtggatga 
      aatggtggg aataatata tcgacctaa aacgcaaga 
      aactagaca aacgtatga agtgctttt gagtttttt 
      gcaagaagc attggggga tcaaccaag agatattct 
      ttgtggtgc agcagatga ggtcttaac agtattaaa 
      gaacgacag gttgaagga gaaagaaaa gaaaaagga 
      gactgaaat gctgttagg ctctattgc tgaggagag 
      atttgcttt actcgttaa tctgggtaa aaaaattac 
      tgattttgg aaatgaaga aaataaaat atctatgaa 
      tgaagaaaa cattgatga aacttatgg aattaatgt 
      tcagtttga agaatcaga ggaggaaga tgatgaaga 
      catgtatgg agaagttcg cgaggatat ggatgatga 
      agaaggaga agaagctaa agaggatag tgctataca 
      tgcagagaa tcttggtgg tgttgaaga aatgaaaaa 
      ggaaaaagc acttcatcc tcttgatat agacgctta 
      ttggctgca aaggcgcct ttcaaaaat ttatgacga 
      tgcaatggt ttcgcaggc aaaagcggc ggaagtgtt 
      aaatgttct tcgcgatgc tggagatga tcgagagtg 
      cgaaaatca acttgtttt actactagg ttacgattg 
      ctttgactt tattaaaca gttgaaaaa acataggca 
      gatgatttt atattgcac attattggc caaatcaca 
      gagtgaatc agaaagaca gaaaatcaa agataaaat 
      gtctgaaga tcccagtct tactcgtat tttgcgtct 
      tctggaaac cggaaaagg ggatgatga tggtgaaga 
      cgaagaatc aacttcaag atcatcaag ccgaaaaga 
      tcaatcgga tgagatgga tacaggtag tggagcaca 
      agttgctgg atcaagaca cctacttga cttggaaga 
      tttggtgtt tacgcaggg atcgcatta catggcaaa 
      taaacgctg tcagttacc ggatggatc tttcaggaa 
      gcagaggaa aggatacga agaggtaca cgttcctgc 
      tctaaaacc taaaccgtt tggagataa cgaaaagtt 
      acaatcaat tgatcagtt acccaaata cgttcagcc 
      tgtttttga aggcttcaa aacactaaa tcgtattca 
      gagtcgctt atataaaac agcactcga atcagatga 
      gaatttact cttgtgtgc acctacagg tgccggtaa 
      aaccaacgt agcactatt gtgtatgat gcgtgaaat 
      aggcaaaca catcaacac agatggtac aataaacgc 
      cgatgactt taagatcat atacgttgc accaatgcg 
      ttctctcgt acaagaaat ggtcggtaa ttttgggaa 
      aagattatc gagttacaa tatcactgt atcagaatt 
      gactggtga tcatcagtt aactagaga acagatcgc 
      tgggacaca aatcatcgt atgcacccc tgaaaagtg 
      ggatattat aacacgaaa aggaggcga aaaaacatt 
      cacttcgtt ggtaagact aattattat cgacgaaat 
      tcatttgtt acacgatga gagaggtcc ggttctgga 
      agctttggt tgctcgaac aattcgcat gattgaatc 
      gactcaaga agacgtacg tctggttgg tctttcggc 
      tactttacc taattacca agatgttgc cgcgtttct 
      tcgagtcaa atcagatac tggtctttt ttatttcga 
      taatagttt tcgaccagt tgctttgga acaacaata 
      tatcggagt gacggagaa aaaggcttt gaaacggta 
      ccaagtaat gaatgagat tgtttatga aaaaaccat 
      ggaacatgc aggacgcaa ccaagtgtt aattttcgt 
      tcattcgag aaaggagac gggaaaaac agcgcgtgc 
      gatacgtga tatgtgttt ggagaagga tactttagg 
      acagttttt gaaagaggg atcggcttc aatggaagt 
      tttgcgaac cgaagcgga acaagtgaa aaatcatga 
      attgaagga tttgttacc gtatggatt tgcgataca 
      tcatgccgg aatgacgag ggttgacag gacattggt 
      ggaagattt gtttgcaga taggcatat acaggtgct 
      ggtgtcaac tgcaacact tgcctgggg cgtaaactt 
      accagccca cacagtcat aataaaagg aacccaaat 
      ttacaatcc agaaaaagg cagatgggt tgaattggg 
      tgctttgga cgttcttca aatgttagg tcgtgccgg 
      ccgacctca atacgatac cagaggtga aggcatatt 
      gataaccaa tcacagcga actgcaata ctatctatc 
      actattgaa ccaacaatt acccatcga atctcaaat 
      gatatcaaa attatcaga catgttaaa cgctgagat 
      tgttttggg aaccgtaca aaatgtacg agatgccgt 
      cacatggtt aggttacac gtatttata cattcgaat 
      gcttcgaca accaacttt gtacggtgt ttcacatga 
      tcatttcaa acaagatcc gttattgga acaacatcg 
      agctgattt aatacatac agctgcatt gtatctcga 
      tcgtagtgg tttggttaa atatgacag aaaaaccgg 
      tcagtttca agtaaccga actgggacg catttcctc 
      gcattatta ttgcaccca cgaaacgat gcaaactta 
      caatcaatt gctcaaacc aatgttgag cgaaattga 
      attgttccg cgttttttc cctatcgtc tgagttcag 
      gaatataac agtccgtga ggaagagaa acttgagtt 
      gcaaaaatt gatggaacg tgttccaat cccgatcaa 
      agaaagtat cgaagaacc aagtgccaa agtaaacgt 
      actgttaca agcgtacat ttctcaact aaaactgga 
      aggtttcgc acttatgtc cgacatggt ttacgtaac 
      acaatcagc ttcgcgttt aatgcgtgc tatttttga 
      gattgtttt acacagagg ttgggcaca attggccga 
      taaatcgtt agctttgtg taaaatgat tgataaaag 
      aatgtggca gtcgatgtc accgttgcg ccaatttcg 
      caaaatgcc ggaagagat tgtgaagaa aattgaaaa 
      gaaaaattt cccatggga acgtttata cgatttggg 
      accgaacga aatcggcga attgattcg ggtcccaaa 
      gttgggcaa gactatcca taaatacgt tcatcagtt 
      ccccaaact tgaattatc aacgcatat tcaaccaat 
      taccaggtc aatgttgaa ggttgaatt gaccataac 
      gcctgactt tcagtggga tgagaaact tcatggagc 
      gtctgaagc gttttggat tttggtgga agatgtgga 
      ttcagaggt tattctcca ccatgagta ttttttgct 
      caaagcgaa gtattcaca agacgagca tttggttaa 
      attttttgt cccgatttt cgaaccttt gccacctca 
      gtacttctt acgtattgt gtcagatcg atggatcgg 
      agccgagac acaattacc agtttcttt ccgacattt 
      gatactacc agagaagaa tttcccacc aactgaact 
      acttgactt gcaaccttt accagtcac tgccttacg 
      gaacgataa attcgagtc gttgtataa cgacaagtt 
      cccgcagtt taatccaat tcagacgca agtcttcaa 
      ttcggttta caacggtga tgataatat ttttattgg 
      tgccccgac cggttctgg aaaaactac aatcgctga 
      atttgcgat acttcgcct ttttgataa aaacccgga 
      aggtcggtg cgtttatct cgttccgaa agatgcatt 
      agctgagtt aacgtttgc cgattggca caataaatt 
      tgggcaaat tttgggcaa gaaagtagt gctcctaac 
      tggtgaaac cgggactga tttgaagtt actggcaaa 
      aggtcagat tgttataag cacggccga aaaatggga 
      tgtattgtc gcgaagatg gaaacaaag aaagaatgt 
      ccaaaatgt gaatttgtt tatcgttga tgagttgca 
      tttgattgg tggggagga tgggcctgt tattgaagt 
      tgtgtgttc ccgcatgcg atatatttc ctcacagat 
      tgagaaacc gatcaggat tatagcatt aagtgcgtc 
      gcttatgga ttatcgaga tgtgtctca atggttggg 
      ttgtaatgc caatgcaac gtttaattt tcatccgag 
      tgtgaggcc tgtaccgtt ggaattgca tgtacaggg 
      gattaatat tacacataa tgcgtcgag acttatcgc 
      aatggcgaa gccggttta taatgctgt ggtgaggta 
      tagtccaca taagccggt tattgtttt cgtaccgac 
      gaggaaaca atcacgttt gactgcaat tgatttact 
      tacgtatgc tgcgtctga gggacagtc taataagtt 
      tttccatgc ggaggaaga ggatattaa accgttctt 
      ggatcggat gactgataa aactttgaa ggaaacgct 
      ttcccaagg cgttgcata catgcatga aggtttgac 
      agcaagtga tttacgcct agttgaaca gttgtttga 
      ctcgggggc ggtacaaat tgctgttgt tagtaagga 
      tttgtgttg gaccgtgaa catttattc ttacttggt 
      tatcataat ggacacaca attttacaa tggaaaagt 
      tcattccta cgaagatta tcccattac cgatgtgtt 
      acaaatggt gggacgtgc taatcgtcc attggagga 
      tgatgatgc gaaatgtgt actaatgtg tcaaagtac 
      caagaagga tttcttcaa gaaattcct aagtgagcc 
      gttaccggt tgaaagtca tttggatca tagattaca 
      tgaccattt caatgccga aatcgtaac aaaaactat 
      cgaaaataa acaggacgc tgttgatta tttgacttg 
      gacatttct gtatcgaag acttacaca aaatccaaa 
      ctattacaa tttgcaagg agttacgca cagacatct 
      ttctgacca tttgtcaga gttggtgga aaatacttt 
      gtccgattt ggaacagtc aaaatgtat cagcgtgga 
      agaggacat ggattgtgt gccacttaa tttgggaat 
      gattgcggc atattatta catcaatta cacaactat 
      tgaactatt cagtttgtc actgaacag caagaccaa 
      aattcgtgg tttattaga aatcatatc gtcggcagc 
      cgaatatga agatctttc cgtacgtca tcgcgagga 
      taacattct tcgccaatt ggcaacaaa attaccaaa 
      taaactgac atcttccag tggaagtca accgaaata 
      taacgaccc acacgtcaa aacaaatct tctcattca 
      agcacattt gtgtcgact tcagcttgg tgctgagct 
      tcaaggcga tacagaaat tgtactaag caaagcaat 
      tcgacttat tcaagcttg tgttgatgt acttagttc 
      aaatggatg gctttcgcc agctgtggc cgctatgga 
      attggctca aatggtgac acaagcaat gtggagtaa 
      agattcgta tttgaagca attgcctca ctttacgac 
      agaaattat taagaggtg tacggataa aggagtcga 
      aaccgtgtt tgatataat ggaattgga agatgaaga 
      caggtcaaa gctgttgca gttgagcga tagtcaaat 
      ggctgatgt tgccagatt ttgtaatcg gtatccaaa 
      tattgaatt aagttacga agttttgga taaagataa 
      gatacattc cgggtcttc agtgcatgt agctgtgca 
      acttgagag ggaggatga agtgaatgg gcctgtcat 
      tgcgccctt tttcccaca gaaacgtga agaaggatg 
      gtgggttgt tattggtga tccgaaaac caattcctt 
      gctctccat taaacgcct tactcttca acaaaaagc 
      tagagtcaa gttggattt tgttgcccc tagtccagg 
      acaccataa ttacacttt atattttat gagtgatgc 
      gtatttggg ttgcgatca agaatataa attttcaat 
      agatgttgg tgattttga aagcagtga aagtgagtc 
      agagtgaac gagaaatat ttagttgag aataggtaa 
      acttatttt gttatttaa gattcgtac aataaaaag 
      tatgttttt ct