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Gene information
General information
TC033913
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016121,TC016120
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451646.1: 98156..125190
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033913
    iBeetle screen iB_02552

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033913 RA
      MTGIVIKSK VAKLMTDES ANRTDVSAV PIKKHFNVG 
      IVGGDVAQD GQFPWQVAV YFDTSEGSF FCGGALIDT 
      RWVLTAGHC TYHATAFTL HLGANSLVD ADPNRVTLG 
      ASHAVVHPD YKPSDLEND IGLIRIDTV YETNDHIKV 
      ISLDTSDIG GAEPVTVSG WGASGDWDG VVNDLNFVG 
      LKTITNDEC KAIYGEQTI TDGMLCAVG ETIEGTCNG 
      DSGSPLITG SGDKALHVG VVSWASAHG CETNHPSGY 
      TRTAKYYDW IKSVVWPNQ TSTV
    CDS sequence
    • >TC033913 RA
      atgactggg attgtaatc aagtcaaaa gtagcgaaa 
      ctgatgact gatgagtcg gcaaatcgg acagatgtt 
      agtgcagtt ccaatcaaa aaacacttc aatgttggg 
      atcgttggc ggtgatgtt gcccaagat ggtcaattt 
      ccttggcaa gtggctgtg tatttcgac accagtgag 
      ggttcgttt ttctgtgga ggggctctg attgatacc 
      agatgggtt ctaacggcc ggacattgc acctatcac 
      gcaactgca tttacactt catttgggg gcaaattct 
      ttggttgac gcggatcca aatcgagtg accttgggg 
      gccagccat gcggtggtt cacccagat tataaacct 
      tcagatttg gagaacgac attggactt atccgaatt 
      gacacagtt tatgaaact aatgatcat atcaaagtc 
      atttctctt gatactagt gatataggg ggtgctgaa 
      ccagttact gtaagtggt tggggagct tccggtgac 
      tgggatggt gttgtcaat gaccttaat ttcgtcggt 
      ttgaaaacc ataaccaac gacgaatgt aaggccatt 
      tacggcgaa caaaccatc accgatggt atgctgtgt 
      gctgttggt gaaacgatt gaagggaca tgcaatgga 
      gatagtgga agtcctttg ataactgga agtggagat 
      aaagccctt catgttggt gttgtaagt tgggcaagt 
      gcccatggg tgtgaaaca aatcatccc tcgggttat 
      acgaggact gctaaatac tacgactgg attaaaagt 
      gtggtctgg ccaaaccaa actagcact gtttga
    mRNA sequence
    • >TC033913 RA
      tgatgcaag cgagtagaa cgggtgaag gggttgaga 
      ttttaagta tgtgggtgt aaattcaaa agttcgggt 
      cggggtgga aatggaaca gcgaaagag gtaattttg 
      tgaaaattt tggggaatg taacaataa aaaactagg 
      acaatataa cgataaaac tgttgatga gagcagttc 
      agtcataat ccccatgac tgggattgt aatcaagtc 
      aaaagtagc gaaactgat gactgatga gtcggcaaa 
      tcggacaga tgttagtgc agttccaat caaaaaaca 
      cttcaatgt tgggatcgt tggcggtga tgttgccca 
      agatggtca atttccttg gcaagtggc tgtgtattt 
      cgacaccag tgagggttc gtttttctg tggaggggc 
      tctgattga taccagatg ggttctaac ggccggaca 
      ttgcaccta tcacgcaac tgcatttac acttcattt 
      gggggcaaa ttctttggt tgacgcgga tccaaatcg 
      agtgacctt gggggccag ccatgcggt ggttcaccc 
      agattataa accttcaga tttggagaa cgacattgg 
      acttatccg aattgacac agtttatga aactaatga 
      tcatatcaa agtcatttc tcttgatac tagtgatat 
      agggggtgc tgaaccagt tactgtaag tggttgggg 
      agcttccgg tgactggga tggtgttgt caatgacct 
      taatttcgt cggtttgaa aaccataac caacgacga 
      atgtaaggc catttacgg cgaacaaac catcaccga 
      tggtatgct gtgtgctgt tggtgaaac gattgaagg 
      gacatgcaa tggagatag tggaagtcc tttgataac 
      tggaagtgg agataaagc ccttcatgt tggtgttgt 
      aagttgggc aagtgccca tgggtgtga aacaaatca 
      tccctcggg ttatacgag gactgctaa atactacga 
      ctggattaa aagtgtggt ctggccaaa ccaaactag 
      cactgtttg agaaatttg agttagact tttaatgta 
      gttgtagcg aaaataaat gtagttgta atgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY