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Gene information
General information
TC016110 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1518: 176845..190765
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016110
    iBeetle screen iB_02551
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016110 RA
      MADQESCKD IQLLKRTRI SVFVDEALP DILVVTYEF 
      GVKIFKGVL LDSTKRNLP CGIPNLNPA FKVSVKPKH 
      EDDSLYSVN QRFAYTEPS TAKKKNVQI PSKYKNNKM 
      TVRLRPRQV LCSKCKGIC NENSENVSR KRKPTESVL 
      SPPVKRGLN APITRSVSI HSELNKKKL GEATLVPKL 
      TRLTPQEEK DAMNGKIVT FKKPLTAVS ENTQKPSSD 
      SDSDESDTG RRILRKKRS VGSMEDLWD ESVFEENAN 
      KNNNVNTRT IKISYGPQG EGTVLKIPA QIENLKEEE 
      IEEKSDNKA ARKALKKAK KEARRKVVF TLGGSSPRY 
      TVGSASPRH GLGNNSPRY MCPDLSIPR RRKHKMKHK 
      KKHKEDRKH REGEVSSTQ EDPKEQCIT QKLSINLKR 
      LNNTYASYP TAPSDKTPE ENTSSSDEH SEQVPDFPP 
      PTPPIMLRI NSQTLSSAP TADGGRMLV GDVVWGKIH 
      GFPWWPGKV LTITNCGGQ GPQAHVAWY GSSTSSLMQ 
      CDQLSPYLE NFKIRYNKK KRGPYKEAI KQATLEAKE 
      NAEIKMRQP LGNSPSHNI IQQVVPPAL ASPREIDVV 
      S
    CDS sequence
    • >TC016110 RA
      atggcggac caagaaagt tgtaaggac attcagtta 
      ttaaaaagg acaagaatt tcagttttc gtcgatgaa 
      gccttacca gacattcta gtagtaacg tacgaattc 
      ggcgtgaaa atattcaaa ggagttctc cttgattcc 
      accaaaaga aacctcccc tgtggcatc cccaacctc 
      aaccccgca tttaaagtg tcggtaaaa ccgaaacac 
      gaagatgac agtctttac tcagtcaac caaagattc 
      gcctacaca gagcccagc actgcaaag aaaaaaaac 
      gttcaaatc ccgagtaaa tacaagaac aataaaatg 
      acggtgagg ctgcgccct cgtcaagtc ttgtgttca 
      aaatgcaaa ggaatttgc aacgaaaac tcggaaaat 
      gtctcgcgg aaacgcaaa ccgacggaa agtgttttg 
      tctccgcct gtcaaaagg gggctcaat gcgccgatc 
      actcgttca gtgtcgatt cactccgaa ctcaataag 
      aagaaactg ggcgaagcc accttagtt ccgaaactt 
      acgcgtttg actcctcag gaggagaaa gacgccatg 
      aatgggaaa attgtcaca tttaaaaag cccctcact 
      gctgtttcg gaaaacacc cagaagccc tcctctgac 
      tcagactcg gatgagagc gacaccggc cgcagaatc 
      ctccgaaag aaacgcagc gttggctca atggaagac 
      ctctgggac gagagtgtc tttgaagag aacgccaac 
      aaaaacaac aacgtcaac acgcgcacg attaaaatc 
      tcgtatggc ccccagggc gaaggcaca gtcctgaaa 
      atccctgca cagattgag aacctcaaa gaggaggaa 
      attgaagaa aaaagtgat aataaagcg gcgcgaaaa 
      gcactaaaa aaagccaaa aaggaggca cggagaaaa 
      gttgtgttc actttgggt ggctcttcg ccaaggtac 
      accgtcgga agtgcgtcc ccgagacat ggcttaggg 
      aacaacagc ccaaggtac atgtgtcca gatttgagc 
      ataccgagg aggaggaag cacaaaatg aagcataaa 
      aagaagcat aaggaggat cgtaaacac agggagggt 
      gaggtaagt tcaactcaa gaggaccca aaagagcaa 
      tgcatcacc caaaagctg tcaattaac ttaaaacga 
      cttaacaac acgtatgca tcttatcct actgccccc 
      tctgacaaa acccccgaa gagaacacg tccagttcc 
      gatgaacac agcgaacaa gttccagac ttccctcca 
      ccaacgcca cctataatg ttgcgcatc aattcgcaa 
      acattatcg agcgcaccg acagcagat ggaggccgt 
      atgttggtc ggcgatgtc gtctggggc aaaatccac 
      ggctttcct tggtggcca ggcaaagtg ctcaccatc 
      acgaactgt ggaggccaa gggccccaa gcgcatgtc 
      gcatggtac ggttcctca acgtcgtca ctaatgcag 
      tgcgatcag ttaagtcca tatttagaa aattttaag 
      atacgttac aataagaaa aaaaggggg ccatacaaa 
      gaagccatc aagcaggcg acgctggaa gcgaaggaa 
      aacgccgaa ataaagatg cgccaacct ttaggaaac 
      tcaccttcc cataatatc atccaacag gtggtgcca 
      cctgctttg gcgtcgcct agagaaatt gatgttgta 
      tcataa
    mRNA sequence
    • >TC016110 RA
      ccataaaat aaattcgtc cacagccca aaactaact 
      tttcctttc atttccttt aaacaattt ccatttcct 
      gtctgaata aactccaag tttcgttaa tttccccaa 
      tcccgaatt tccaagcac attttcacg tcggtttta 
      atttccatt ttcaaatat ggcggacca agaaagttg 
      taaggacat tcagttatt aaaaaggac aagaatttc 
      agttttcgt cgatgaagc cttaccaga cattctagt 
      agtaacgta cgaattcgg cgtgaaaat attcaaagg 
      agttctcct tgattccac caaaagaaa cctcccctg 
      tggcatccc caacctcaa ccccgcatt taaagtgtc 
      ggtaaaacc gaaacacga agatgacag tctttactc 
      agtcaacca aagattcgc ctacacaga gcccagcac 
      tgcaaagaa aaaaaacgt tcaaatccc gagtaaata 
      caagaacaa taaaatgac ggtgaggct gcgccctcg 
      tcaagtctt gtgttcaaa atgcaaagg aatttgcaa 
      cgaaaactc ggaaaatgt ctcgcggaa acgcaaacc 
      gacggaaag tgttttgtc tccgcctgt caaaagggg 
      gctcaatgc gccgatcac tcgttcagt gtcgattca 
      ctccgaact caataagaa gaaactggg cgaagccac 
      cttagttcc gaaacttac gcgtttgac tcctcagga 
      ggagaaaga cgccatgaa tgggaaaat tgtcacatt 
      taaaaagcc cctcactgc tgtttcgga aaacaccca 
      gaagccctc ctctgactc agactcgga tgagagcga 
      caccggccg cagaatcct ccgaaagaa acgcagcgt 
      tggctcaat ggaagacct ctgggacga gagtgtctt 
      tgaagagaa cgccaacaa aaacaacaa cgtcaacac 
      gcgcacgat taaaatctc gtatggccc ccagggcga 
      aggcacagt cctgaaaat ccctgcaca gattgagaa 
      cctcaaaga ggaggaaat tgaagaaaa aagtgataa 
      taaagcggc gcgaaaagc actaaaaaa agccaaaaa 
      ggaggcacg gagaaaagt tgtgttcac tttgggtgg 
      ctcttcgcc aaggtacac cgtcggaag tgcgtcccc 
      gagacatgg cttagggaa caacagccc aaggtacat 
      gtgtccaga tttgagcat accgaggag gaggaagca 
      caaaatgaa gcataaaaa gaagcataa ggaggatcg 
      taaacacag ggagggtga ggtaagttc aactcaaga 
      ggacccaaa agagcaatg catcaccca aaagctgtc 
      aattaactt aaaacgact taacaacac gtatgcatc 
      ttatcctac tgccccctc tgacaaaac ccccgaaga 
      gaacacgtc cagttccga tgaacacag cgaacaagt 
      tccagactt ccctccacc aacgccacc tataatgtt 
      gcgcatcaa ttcgcaaac attatcgag cgcaccgac 
      agcagatgg aggccgtat gttggtcgg cgatgtcgt 
      ctggggcaa aatccacgg ctttccttg gtggccagg 
      caaagtgct caccatcac gaactgtgg aggccaagg 
      gccccaagc gcatgtcgc atggtacgg ttcctcaac 
      gtcgtcact aatgcagtg cgatcagtt aagtccata 
      tttagaaaa ttttaagat acgttacaa taagaaaaa 
      aagggggcc atacaaaga agccatcaa gcaggcgac 
      gctggaagc gaaggaaaa cgccgaaat aaagatgcg 
      ccaaccttt aggaaactc accttccca taatatcat 
      ccaacaggt ggtgccacc tgctttggc gtcgcctag 
      agaaattga tgttgtatc ataatgaag catttttct 
      cctccttgt ttggtggaa catacaggt tttgttgta 
      gcgtgttta aatcacgct catcaccct gtatatact 
      aaatgtgtt aatagctac tactgcgca ttgatgtat 
      tctttttta taaagtact ttatattta tacagaaat 
      atttgtatg cagcaggcg taacatgtt tcttatttt 
      aattcctga tattgtatc atctcttgt aaagaatat 
      tttttgtga taactttgg atgattgtt ttcatattg 
      aagtgtttt attcaagat ttttatagg gttaagtca 
      tagttacta ctatctgtt aaacaataa tcaatatat 
      ctgtgattt tattccttt agaaacatg taagtagta 
      aaattaaat taataaagt catttggta cta