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Gene information
General information
TC031492
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC016062
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1937436..1939416
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031492
    iBeetle screen iB_02548

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    legs slightly shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs femur potentiallyfused - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs tibiotarsus potentiallyfused - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs pretarsus bent - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not completely molted - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection



    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (16 dpi)
    Days post injection


    Comment: head and leg defects cannot be accurately scored because of the metamorphosis block related irregularities

    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 9
    Comment: probably secondary effect
    maxillary palp slightly size increased - Number of animals showing the phenotype: 8

    legs orientation irregular - Number of animals showing the phenotype: 10

    Comment: probably secondary effect
    elytra anterior rim broader - Number of animals showing the phenotype: 10
    wings distal blistered - Number of animals showing the phenotype: 9

    Comment: blisters are at defined positions and of similar size
    wings median blistered - Number of animals showing the phenotype: 9

    Comment: more proximal blister looks like in the hindwing
    wings shape irregular - Number of animals showing the phenotype: 10
    oblique dorsal thoracic muscles need term slightlyshortened - Number of animals showing the phenotype: 7

    Adult morphology
    wings blistered - Number of animals showing the phenotype: 5

    Comment: some show blisters, not present in pupal stage

    iBeetle screen iB_06361

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 2
    ovary without detectable defects - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    legs pretarsus position irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs trochanter size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Just 8 larvae on slide, but all show the leg phenotype.
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    Pupal morphology
    antenna orientation irregular - Number of animals showing the phenotype: 8

    elytra shortened - Number of animals showing the phenotype: 8

    elytra partially distalblistered - Number of animals showing the phenotype: 8

    legs orientation irregular - Number of animals showing the phenotype: 8
    wing margin shape irregular - Number of animals showing the phenotype: 8
    elytra shape irregular - Number of animals showing the phenotype: 8
    oblique dorsal thoracic muscles potentially shortened - Number of animals showing the phenotype: 8

    Comment:
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031492 RA
      MHFSIPDTQ ELNEGNGST YIGYNIHIN GIFHCTVRY 
      KQLHNLHEQ LKKEFGNDN LPPFPPKKL LPLTGGQLE 
      DRRALLEKY IQTVGQDPK LVSSELLVG FLLSAQQET 
      TCEKRQEVN LDVYTSNNY QIPLTVSTF DRTEQVLIK 
      VFKQINLPL QYLQYFSLY LIKKDNNGD VVVLRKLLS 
      FESPYMTHK TVRDSNKIV IRKSYWDCN YDQELMTDP 
      VALNLLYVQ VVSDVEKGW ICCTRDMKS QLSNLQSRL 
      AKREYIELA RTLKYYGFM QFMPCYCDF PKPQTKVLI 
      AIGDQELSM RLLGTGQFV KEGSFKVTR MRCWRITAT 
      HNKQDMAAN SHTNASSGL ELSFEYLMS KDKLQWITV 
      SSEQAILMS VCLQSLVDE LLMKKNGVQ KKQPPMNKG 
      NWSYMKRDG SCHLISLDD VNSNSREDG SAQKYQESF 
      SIKKLQEKF SSVSFKTGK EFIENHAFE GIGDDDL
    CDS sequence
    • >TC031492 RA
      atgcatttt tcaattcct gacactcaa gaattaaac 
      gaaggaaat gggtcaacc tacatcggt tacaatatc 
      cacatcaac ggcattttt cactgtact gtgcgttac 
      aagcaattg cataattta cacgagcaa ttaaagaaa 
      gagttcggt aacgataat cttccacca ttccctcca 
      aaaaaactg ctgccatta acagggggt caattagag 
      gatagacgt gcactgctg gaaaagtac atccagaca 
      gtggggcaa gatccgaag ctggtttct tctgaactc 
      ctggtagga tttctgctt tcggcccag caagagacg 
      acgtgcgaa aagcgacaa gaagttaat ttagacgtg 
      tatactagt aataattat caaattcct ctcactgtg 
      tccacattt gatcgtact gagcaagta ctcataaaa 
      gtatttaag caaattaat ctccccctg cagtacttg 
      caatacttt tctttgtat ctcatcaag aaagacaac 
      aacggcgac gtggtcgtt ttgaggaag ctcctcagc 
      ttcgagtcg ccctacatg actcacaag actgtccga 
      gattcaaat aaaattgtg ataaggaag agctactgg 
      gactgcaac tacgatcaa gagctcatg acggatcct 
      gttgcgctg aatcttcta tacgtccaa gtagttagt 
      gatgtagaa aaagggtgg atatgttgc accagagac 
      atgaagtcg caactgtcg aatctccag tcgagatta 
      gccaagcgg gaatacatc gagctagcg cgaacgttg 
      aaatactac ggttttatg caattcatg ccatgctac 
      tgtgatttt ccaaagcct caaaccaaa gtccttatt 
      gcgataggt gatcaagaa ctgagtatg cgattactt 
      ggcactggg cagttcgtt aaggaaggg agtttcaaa 
      gtcaccaga atgagatgt tggcggatc acagcaaca 
      cataataag caagatatg gcagcaaat agtcacacg 
      aacgcgagt tccggcctg gagctctct tttgagtat 
      ttgatgtct aaggacaag ctgcagtgg atcactgtg 
      tcaagcgag caggcgatt ttgatgtct gtgtgtctc 
      cagtcgtta gttgacgag ttgctaatg aagaagaac 
      ggcgttcag aagaaacag cccccgatg aataagggg 
      aattggtcg tacatgaag agggatggg tcttgccac 
      ctaatctcg ttagatgat gtcaattca aactcaaga 
      gaggacggt tctgctcaa aaataccaa gagtcgttt 
      tcaattaag aaactccaa gagaaattt tctagtgta 
      tcatttaag acagggaaa gaatttatt gaaaatcac 
      gcatttgaa ggaataggc gacgatgat ttatag
    mRNA sequence
    • >TC031492 RA
      atggtggac cgtcaattt tagtaaacg aagaaaaat 
      cgtgtatat ttttttgtg tgttttgtt acgttgtgc 
      ctaatttag taaaaatgc atttttcaa ttcctgaca 
      ctcaagaat taaacgaag gaaatgggt caacctaca 
      tcggttaca atatccaca tcaacggca tttttcact 
      gtactgtgc gttacaagc aattgcata atttacacg 
      agcaattaa agaaagagt tcggtaacg ataatcttc 
      caccattcc ctccaaaaa aactgctgc cattaacag 
      ggggtcaat tagaggata gacgtgcac tgctggaaa 
      agtacatcc agacagtgg ggcaagatc cgaagctgg 
      tttcttctg aactcctgg taggatttc tgctttcgg 
      cccagcaag agacgacgt gcgaaaagc gacaagaag 
      ttaatttag acgtgtata ctagtaata attatcaaa 
      ttcctctca ctgtgtcca catttgatc gtactgagc 
      aagtactca taaaagtat ttaagcaaa ttaatctcc 
      ccctgcagt acttgcaat acttttctt tgtatctca 
      tcaagaaag acaacaacg gcgacgtgg tcgttttga 
      ggaagctcc tcagcttcg agtcgccct acatgactc 
      acaagactg tccgagatt caaataaaa ttgtgataa 
      ggaagagct actgggact gcaactacg atcaagagc 
      tcatgacgg atcctgttg cgctgaatc ttctatacg 
      tccaagtag ttagtgatg tagaaaaag ggtggatat 
      gttgcacca gagacatga agtcgcaac tgtcgaatc 
      tccagtcga gattagcca agcgggaat acatcgagc 
      tagcgcgaa cgttgaaat actacggtt ttatgcaat 
      tcatgccat gctactgtg attttccaa agcctcaaa 
      ccaaagtcc ttattgcga taggtgatc aagaactga 
      gtatgcgat tacttggca ctgggcagt tcgttaagg 
      aagggagtt tcaaagtca ccagaatga gatgttggc 
      ggatcacag caacacata ataagcaag atatggcag 
      caaatagtc acacgaacg cgagttccg gcctggagc 
      tctcttttg agtatttga tgtctaagg acaagctgc 
      agtggatca ctgtgtcaa gcgagcagg cgattttga 
      tgtctgtgt gtctccagt cgttagttg acgagttgc 
      taatgaaga agaacggcg ttcagaaga aacagcccc 
      cgatgaata aggggaatt ggtcgtaca tgaagaggg 
      atgggtctt gccacctaa tctcgttag atgatgtca 
      attcaaact caagagagg acggttctg ctcaaaaat 
      accaagagt cgttttcaa ttaagaaac tccaagaga 
      aattttcta gtgtatcat ttaagacag ggaaagaat 
      ttattgaaa atcacgcat ttgaaggaa taggcgacg 
      atgatttat agattgacg aattaagat tgttttgct 
      ttggtaaat gaatgttgt tacaacgtg ggttttttt 
      aaggtgcta ttttccaat gatgtccaa aagaattat 
      gccatttac aataactaa attaccaaa atcatacat 
      tgtgaggga gaaattgac ctcatacag tccatttta 
      tgtgccaaa cttagaaat aataaacaa acatatttt 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY