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Gene information
General information
TC016055 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1892453..1894571
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016055
    iBeetle screen iB_02547
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    Fertility
    egg production: number <50% of expected -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016055 RA
      MFRTPKRGK LTNSSDDHV NITPPNRVL KPKILNSLD 
      KGPVNGSSS PGTENSVLS PGETRRGRP RAEMLNTLI 
      LEGSTSPSS IKCTYCNRV FPREKSLQA HLRTHTGER 
      PYECDYPRC TRKFAQSGQ LKTHQRLHT GEKPFICSS 
      PNCDKRFTH ANRHCPEHP DKQLKRFTS PGSSFEKKF 
      DEGHNAEVK QWLMKLEKR NNKRPSSLD GLETTPKKS 
      RIKREVSST YSLSNDGIE NSPKSELDD NISVNSENF 
      ENGHELISP PRSSLHNFE LPKGPKKRW LQAVQDQKM 
      FDSTQDLAR PLNWGEDGP SSDGDEVKI EPIEFGASE 
      PKVVVENQK RPTVLVCVQ GGKSKQITS DDMQGAIAL 
      VELKNGQKS CVNYNYRL
    CDS sequence
    • >TC016055 RA
      atgtttcgt actcccaaa cgtggtaaa ttaacgaat 
      agtagtgat gatcatgtg aatataaca ccgccgaat 
      agagtattg aagcctaaa atattaaat tccttggac 
      aaaggaccg gttaacggt agctctagt ccaggaaca 
      gaaaattca gttctttcg cctggcgaa actaggaga 
      ggccgtccg agggccgaa atgttaaat actcttatc 
      ctagaagga tcgacctct cccagctcc ataaaatgt 
      acttattgt aaccgagtt tttccacgc gaaaagtcg 
      ctccaagca cacttacga acccacact ggcgaaagg 
      ccctacgag tgcgattat ccacggtgc acgagaaaa 
      ttcgcccaa tcgggccag ttaaagaca caccaaaga 
      ctccacaca ggcgaaaaa cccttcata tgttcctct 
      cccaactgt gacaaacgc ttcactcac gccaaccgg 
      cattgcccc gaacaccct gataagcaa ttaaaacgg 
      ttcacttcg cctggaagc agctttgag aagaaattc 
      gatgaaggt cacaacgct gaggtcaag cagtggctg 
      atgaaactg gaaaagcgc aataacaag aggccaagc 
      agcttagac ggcctcgag acaacgccg aaaaagtcg 
      cggatcaag cgcgaggtc tcctcgact tacagcctc 
      tccaacgac gggatcgaa aactccccg aaatccgaa 
      ctcgacgat aacatttcc gtgaattcg gagaacttt 
      gagaacggc cacgagttg atctccccg cctcggtcg 
      tcgctccac aacttcgag ctccccaaa gggccgaag 
      aagcggtgg ctgcaggcc gtccaagac cagaagatg 
      tttgactcc actcaagac ctggccagg ccgctcaat 
      tggggcgaa gacggccca agttcggac ggcgatgag 
      gtcaaaatt gagccgatt gagttcggg gccagcgag 
      cccaaagtc gtggtcgag aaccaaaaa cgccccacc 
      gttctggtc tgcgtccag ggcggcaag agcaagcag 
      attaccagt gatgatatg caaggtgct atagctttg 
      gtcgagttg aagaatgga cagaaaagt tgtgttaac 
      tataattac agactctaa
    mRNA sequence
    • >TC016055 RA
      aggaggttg tgtggttgt cgtcttgtc tattgtttt 
      attaatttt tttaatgga tttggctaa gatgtgaaa 
      ctcttagat aaccgtgtg tggaatggt gaacatgac 
      agtgtcgta attagtgca tcgtggctg cagaagtcc 
      ctctttcgt taaaggtta attgcaaat ttacagttg 
      tttgttggt gatgttgtc gcgttgtgt tggctttcc 
      tgttgtttc cctccaatt tggcgcgcc atcttcaga 
      gcatcagtt gtaataaat ggaaggcag gtttgtttt 
      ggttggcgt tgtgctgtt ggaaaaatc gtaaaaata 
      gtgacttaa aactgtcta aattatgtt tcgtactcc 
      caaacgtgg taaattaac gaatagtag tgatgatca 
      tgtgaatat aacaccgcc gaatagagt attgaagcc 
      taaaatatt aaattcctt ggacaaagg accggttaa 
      cggtagctc tagtccagg aacagaaaa ttcagttct 
      ttcgcctgg cgaaactag gagaggccg tccgagggc 
      cgaaatgtt aaatactct tatcctaga aggatcgac 
      ctctcccag ctccataaa atgtactta ttgtaaccg 
      agtttttcc acgcgaaaa gtcgctcca agcacactt 
      acgaaccca cactggcga aaggcccta cgagtgcga 
      ttatccacg gtgcacgag aaaattcgc ccaatcggg 
      ccagttaaa gacacacca aagactcca cacaggcga 
      aaaaccctt catatgttc ctctcccaa ctgtgacaa 
      acgcttcac tcacgccaa ccggcattg ccccgaaca 
      ccctgataa gcaattaaa acggttcac ttcgcctgg 
      aagcagctt tgagaagaa attcgatga aggtcacaa 
      cgctgaggt caagcagtg gctgatgaa actggaaaa 
      gcgcaataa caagaggcc aagcagctt agacggcct 
      cgagacaac gccgaaaaa gtcgcggat caagcgcga 
      ggtctcctc gacttacag cctctccaa cgacgggat 
      cgaaaactc cccgaaatc cgaactcga cgataacat 
      ttccgtgaa ttcggagaa ctttgagaa cggccacga 
      gttgatctc cccgcctcg gtcgtcgct ccacaactt 
      cgagctccc caaagggcc gaagaagcg gtggctgca 
      ggccgtcca agaccagaa gatgtttga ctccactca 
      agacctggc caggccgct caattgggg cgaagacgg 
      cccaagttc ggacggcga tgaggtcaa aattgagcc 
      gattgagtt cggggccag cgagcccaa agtcgtggt 
      cgagaacca aaaacgccc caccgttct ggtctgcgt 
      ccagggcgg caagagcaa gcagattac cagtgatga 
      tatgcaagg tgctatagc tttggtcga gttgaagaa 
      tggacagaa aagttgtgt taactataa ttacagact 
      ctaaacttt acgttatga tcttcaaat tatatttgt 
      atgtgatac gttgttcaa atgttcgtc tttattggt 
      cacttatta cgccggcgc cgatcttta aggtccccg 
      atttttatt tatggttag gctctgaag ttaaggata 
      caggttgca gcaattaca ctttatttt atttgttat 
      gtatgctat tgtgttcat gcccattat gttttatca 
      tgtcgcacg ttaattttt aagtacgca cctattaca 
      gcacgcgat tttaattta gtacaattg agacaggtt 
      tgatcagtc agtacaaag acaataatg gttatttta 
      attttaata atccaaaga gtttgtctt agtatcgtt 
      gtgtgctag tttagtgtc gatccttaa cttgaaagt 
      atctttgtt atttatacc taattttaa attgcttta 
      ggaatcgta tgtgatgtg tttttatct aaacagtta 
      tctttatgt tgtaagtat ttattaatg atttggata 
      atataataa cactccttg taagaaata aaaatttaa 
      taaaaac