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Gene information
General information
TC016041 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1843: 209756..213540
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016041
    iBeetle screen iB_02546
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016041 RA
      MSTNAHSIH EVQATTSVS KTPKPDEKR SRTLLNILP 
      KLPSSDSLS NSPGPASPG VSNFIEKSD LNTDRSPET 
      CSKLTRKES YKAQRKNYR KEKKRVTNE LLNSLKDPS 
      VVVLADWLK IRGTLKSWT KLWCVLKPG LLLLYKSPK 
      AKSSHWVGT ILLNSCKVI ERPSKKDGF CFKLYNPLD 
      QSIWAPRGP ENETIGAVV QPLPSSYLI FRAPSQASG 
      MCWLDALEI SIRNDSALV RSVSNKSAS GSTNHETQW 
      NEADYEKHF DLDDVSQPD NGAQLSTGD GEMTDSDSE 
      ISAKEEDID EDPPETPYV PISEEEFGD AGAQVEELA 
      EEHKSLIWY LVKQVRPGM DLSKVVLPT FILEPRSFL 
      DKLTDSYYH VDILSGAVL EDDAFTRMK MVVKWYLSG 
      LYKKPKGLK KPYNPILGE TFRCYWLHP NGSKTFYLA 
      EQVSHHPPV SAFYVTNRQ DGFSISASI LARSKFYGN 
      STSAILDGT AILTLLPRG EDYTLTVPY AHCKGILMG 
      TLTMELGGK VSIDCEKTG YFTEIEFKL KPFLGGVEQ 
      TNCISGRLK LGKETLATI DGYWDGTIY IKDKRTGEQ 
      QVFWNSTPQ VRRQRLTRY SVPVDHQEE FESERLWQH 
      VSAAILRDD MNAATEEKT LLEEAQRAR SKERKAKCE 
      EWVPVHFQQ DLKTGQWVY KHSDLRPWD PRNDLYQYE 
      YNYVIQTRT KHPTPMIRT ASIISVDPQ QGETRSSTL 
      KVTKRKMAP KQVSSDADV NDSATTSEE FHSDSTQSV 
      KKMPLAATN SSIMLAIHD LEQSLQEHG EKISQLQRN 
      INRVLESNM KSRFSPSFI EIIMIVCLL GFFQAVFVY 
      FF
    CDS sequence
    • >TC016041 RA
      atgtccaca aatgcacat tcaattcac gaagtacaa 
      gcgacaact tccgtttct aaaaccccc aaaccggac 
      gaaaaacgt tctcgaact ctgctaaac atcctcccc 
      aaactccct tcaagtgat tcactcagc aatagccct 
      ggaccggca agtcctggt gtttccaac ttcatagag 
      aaatcagat ctaaacaca gacaggtct cctgaaacg 
      tgctccaag ctaacaagg aaggagtcg tataaggca 
      caacgtaaa aactaccgc aaagaaaag aaaagagtt 
      actaatgaa ttactaaac tcacttaaa gacccctca 
      gttgttgtt ttagctgac tggctcaaa atcagaggg 
      actttaaaa tcatggact aagctatgg tgtgtcctt 
      aaacctgga ttgttgcta ctctacaag agccccaaa 
      gccaaaagc agccattgg gtgggcaca attttgcta 
      aattcgtgc aaagtgatt gaaagaccg agcaaaaaa 
      gacggtttt tgttttaaa ctgtataat ccacttgac 
      caatcaatt tgggcccca cgtggtcct gaaaatgag 
      acaattggg gccgttgtt caacccctt ccaagttcc 
      tacttgatc tttcgagcc ccctcacaa gcgtctggc 
      atgtgttgg ttggatgcg ttagaaatt tcaataaga 
      aacgattcg gctttagtc cgctcagtt agtaataaa 
      tcggcttca ggaagcaca aatcatgaa actcaatgg 
      aacgaagct gattacgag aaacatttt gatttagat 
      gatgttagt cagccggat aacggtgct cagcttagt 
      acaggagac ggggaaatg actgattcc gattcggaa 
      atttcggcc aaagaggaa gatattgat gaggatcca 
      ccggaaaca ccgtatgtt cctattagt gaagaggag 
      tttggtgac gcgggagct caagttgaa gagttagca 
      gaagaacac aagtcactt atttggtat ctagttaag 
      caagtgagg ccaggtatg gacttaagt aaagtagta 
      ttgccaact tttatcttg gagccgcgt tcgtttctt 
      gataagtta acagattcg tactaccat gttgatata 
      ctatcggga gcggtactt gaagacgat gcttttact 
      cgaatgaaa atggtagta aagtggtac ctttctggt 
      ttatataaa aaaccaaaa ggtttgaaa aagccatat 
      aatcctatc cttggtgag acgtttcgg tgctattgg 
      ttacacccc aacggttca aaaacgttc tacttagct 
      gaacaagtc tcgcaccat cctcctgtt tctgcattt 
      tacgttact aatagacaa gatggtttt tcaataagt 
      gcaagtatc ttggcacgt tccaaattt tacgggaat 
      tcaacgtcc gctatattg gatggtacc gcaatttta 
      acgttacta ccaagaggt gaagattat acactaacc 
      gtaccatat gcccattgc aaaggtatc ctcatgggc 
      acacttaca atggagttg ggcggaaaa gtgtcaatt 
      gactgtgaa aagactgga tattttacc gaaatcgaa 
      tttaaactt aaacctttt ctgggtgga gttgaacaa 
      acgaattgt atttctggt cgtttaaaa ctcgggaaa 
      gaaacacta gcgaccatt gatggctac tgggatggt 
      actatttac attaaagac aagcgaaca ggtgaacaa 
      caagtattt tggaattcc acaccacaa gttcggaga 
      cagaggtta acaaggtat agcgtcccc gtagaccat 
      caggaggaa tttgaatct gaaagattg tggcaacat 
      gtatctgcg gctatttta agagatgac atgaatgcg 
      gcaactgag gaaaaaact cttctcgag gaagcacaa 
      agagcgcgt tctaaagaa cggaaagcg aaatgcgaa 
      gagtgggtc cctgtgcat ttccaacaa gacttaaaa 
      actggacag tgggtttac aaacattca gatttacga 
      ccgtgggat cctcgaaat gatttatat cagtatgag 
      tataactac gtcatacaa acaaggacg aagcatccg 
      actcccatg attcgcact gctagtata attagtgtg 
      gatccgcaa cagggcgaa acgcgttct tcgactcta 
      aaagtaacg aaacggaaa atggcgcca aagcaagtc 
      tcgtctgac gccgatgta aatgatagt gccacaact 
      tcggaagaa tttcattcc gattccacc cagtctgtg 
      aaaaaaatg ccactagct gcaacaaat tctagtatt 
      atgttggca atccatgac ctcgagcaa agtctccaa 
      gaacatgga gaaaaaata agtcagttg cagcggaac 
      ataaaccgc gtattggaa tcaaacatg aagtctcgt 
      tttagtcct agtttcatt gagattata atgattgtg 
      tgtttgttg ggctttttt caagctgtc tttgtgtat 
      ttcttttag
    mRNA sequence
    • >TC016041 RA
      gtgcaagcg gcaactgaa cgaaaaaag gcctagtca 
      agtgacagt taacgtcgc aatgggtgc gtgttcgca 
      aataatatt aataacgtc aggttttgc aacaatata 
      aacaattaa aagtccttc cagatttgt tcgcaataa 
      tatagagtg taattatat aaacaaagt gatttgtga 
      ttgaaaata tccgtaaac gtcaatcat gatggtagc 
      tgattgttt tgattatca atctaattg ataattcac 
      gtatcaaaa tgaaataaa cacgagggt tccttaatt 
      aagtgaaac tatgcttaa atatcgctt gccaaacgt 
      ttacaatgc ctagcttta acctagaaa tacccgcgg 
      taggtgtaa cccactaag gttaagttt gaaattttt 
      gtaaataag gaaacatgt ccacaaatg cacattcaa 
      ttcacgaag tacaagcga caacttccg tttctaaaa 
      cccccaaac cggacgaaa aacgttctc gaactctgc 
      taaacatcc tccccaaac tcccttcaa gtgattcac 
      tcagcaata gccctggac cggcaagtc ctggtgttt 
      ccaacttca tagagaaat cagatctaa acacagaca 
      ggtctcctg aaacgtgct ccaagctaa caaggaagg 
      agtcgtata aggcacaac gtaaaaact accgcaaag 
      aaaagaaaa gagttacta atgaattac taaactcac 
      ttaaagacc cctcagttg ttgttttag ctgactggc 
      tcaaaatca gagggactt taaaatcat ggactaagc 
      tatggtgtg tccttaaac ctggattgt tgctactct 
      acaagagcc ccaaagcca aaagcagcc attgggtgg 
      gcacaattt tgctaaatt cgtgcaaag tgattgaaa 
      gaccgagca aaaaagacg gtttttgtt ttaaactgt 
      ataatccac ttgaccaat caatttggg ccccacgtg 
      gtcctgaaa atgagacaa ttggggccg ttgttcaac 
      cccttccaa gttcctact tgatctttc gagccccct 
      cacaagcgt ctggcatgt gttggttgg atgcgttag 
      aaatttcaa taagaaacg attcggctt tagtccgct 
      cagttagta ataaatcgg cttcaggaa gcacaaatc 
      atgaaactc aatggaacg aagctgatt acgagaaac 
      attttgatt tagatgatg ttagtcagc cggataacg 
      gtgctcagc ttagtacag gagacgggg aaatgactg 
      attccgatt cggaaattt cggccaaag aggaagata 
      ttgatgagg atccaccgg aaacaccgt atgttccta 
      ttagtgaag aggagtttg gtgacgcgg gagctcaag 
      ttgaagagt tagcagaag aacacaagt cacttattt 
      ggtatctag ttaagcaag tgaggccag gtatggact 
      taagtaaag tagtattgc caactttta tcttggagc 
      cgcgttcgt ttcttgata agttaacag attcgtact 
      accatgttg atatactat cgggagcgg tacttgaag 
      acgatgctt ttactcgaa tgaaaatgg tagtaaagt 
      ggtaccttt ctggtttat ataaaaaac caaaaggtt 
      tgaaaaagc catataatc ctatccttg gtgagacgt 
      ttcggtgct attggttac accccaacg gttcaaaaa 
      cgttctact tagctgaac aagtctcgc accatcctc 
      ctgtttctg cattttacg ttactaata gacaagatg 
      gtttttcaa taagtgcaa gtatcttgg cacgttcca 
      aattttacg ggaattcaa cgtccgcta tattggatg 
      gtaccgcaa ttttaacgt tactaccaa gaggtgaag 
      attatacac taaccgtac catatgccc attgcaaag 
      gtatcctca tgggcacac ttacaatgg agttgggcg 
      gaaaagtgt caattgact gtgaaaaga ctggatatt 
      ttaccgaaa tcgaattta aacttaaac cttttctgg 
      gtggagttg aacaaacga attgtattt ctggtcgtt 
      taaaactcg ggaaagaaa cactagcga ccattgatg 
      gctactggg atggtacta tttacatta aagacaagc 
      gaacaggtg aacaacaag tattttgga attccacac 
      cacaagttc ggagacaga ggttaacaa ggtatagcg 
      tccccgtag accatcagg aggaatttg aatctgaaa 
      gattgtggc aacatgtat ctgcggcta ttttaagag 
      atgacatga atgcggcaa ctgaggaaa aaactcttc 
      tcgaggaag cacaaagag cgcgttcta aagaacgga 
      aagcgaaat gcgaagagt gggtccctg tgcatttcc 
      aacaagact taaaaactg gacagtggg tttacaaac 
      attcagatt tacgaccgt gggatcctc gaaatgatt 
      tatatcagt atgagtata actacgtca tacaaacaa 
      ggacgaagc atccgactc ccatgattc gcactgcta 
      gtataatta gtgtggatc cgcaacagg gcgaaacgc 
      gttcttcga ctctaaaag taacgaaac ggaaaatgg 
      cgccaaagc aagtctcgt ctgacgccg atgtaaatg 
      atagtgcca caacttcgg aagaatttc attccgatt 
      ccacccagt ctgtgaaaa aaatgccac tagctgcaa 
      caaattcta gtattatgt tggcaatcc atgacctcg 
      agcaaagtc tccaagaac atggagaaa aaataagtc 
      agttgcagc ggaacataa accgcgtat tggaatcaa 
      acatgaagt ctcgtttta gtcctagtt tcattgaga 
      ttataatga ttgtgtgtt tgttgggct tttttcaag 
      ctgtctttg tgtatttct tttagttgc agttttatt 
      gtgcaattt atattgtga taaagtctc gtcactgaa 
      ccaatgttt accagttga tttacggga cagtggaga 
      atttttcag tgtaactta ttagcacca aaaattttg 
      ttgtgaatt tgtgtattt ttattacaa actgtgcct 
      tttacgggc tctttgatt atacaattg ttcctggat 
      caaagccct tgtcagttt cattttaaa ttt