Status
Please wait ...
Gene information
General information
TC016037 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid1843: 78696..81150
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC016037
    iBeetle screen iB_02545
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 6 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 11.11% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 22.22% (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_06752
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 3
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC016037 RA
      MFSRFKNAV IGAVSGLET NGIPTPNDP HSYNKQTLA 
      PKFLYGRPA FLNLSEDEV QMSADHRLR PIIHPSMSL 
      PHHAGYAEC VNAGKSKWN EDQAVYRQG VLTRVEYND 
      KQGVQKFSI PYTYYGIFD GHAGVGAAL CAANQLHHI 
      LHEKLVDAQ DDIWIAFSE KKRLKTKPS DLLITGALE 
      AAFKEMDQV IAEDRTKYQ AAGGCTALV ALFILDKLF 
      VANAGDSRA IICKDNKFT LMSMDFTPE NERDRIRRL 
      AEQQPNLLG QEFTSKEYV RPPKSSELG KTVMYRDAH 
      MSGWAYKTL QVDDLKVSV ITGQGKRSR VMGIIGVTR 
      GFGDHDILS LYQRTPIKP FLSSHPEVQ VHKITNVTE 
      NEVLVMGTD GLWDVVCGA KAAEVVAKS LNTFMEKER 
      YVSAATCLV GSARGSLVN EHSWQLKDG KPSSADDIS 
      VFVIPLLPY KLEYEKLLG KYSETAESS
    CDS sequence
    • >TC016037 RA
      atgttcagc cgtttcaaa aatgcagtt ataggtgct 
      gtgagtggt ttagaaacg aacggcatc ccaaccccc 
      aacgacccc cattcctac aacaaacaa actttggcc 
      ccaaagttt ttatacggt cgacctgcg ttcttaaat 
      ttgtctgaa gatgaagtg caaatgtcg gccgaccac 
      agactcagg cctatcatt cacccaagc atgtctctg 
      ccccatcat gcaggatat gccgagtgt gttaacgca 
      gggaagtcc aaatggaac gaggatcag gcggtgtac 
      aggcaaggt gtcttgaca agggtagaa tataatgat 
      aagcaaggt gtgcaaaaa ttttccatc ccgtatact 
      tattatggc atttttgat ggccatgct ggagttggg 
      gcagctctg tgtgcggca aatcagctc caccatatt 
      ttacatgaa aagttggta gatgcccaa gatgacatt 
      tggatagct ttcagtgaa aaaaagagg ctgaaaacc 
      aaacctagt gacttactt ataacaggt gctttagaa 
      gctgctttc aaagaaatg gatcaagtt atagctgag 
      gataggact aaatatcaa gctgctggg ggttgtact 
      gcgttagta gcgctattt attttagat aaattattt 
      gttgccaat gcaggcgat tcacgcgca attatttgt 
      aaagataat aaatttaca ctaatgtca atggatttt 
      actccagaa aacgaacgt gacagaatt agaaggctt 
      gcagaacaa caaccaaat ttattaggg caggagttt 
      acatcaaaa gaatatgtt cggcctccg aaatcgagc 
      gagcttggt aaaacagta atgtatcga gacgctcat 
      atgagtggt tgggcctat aaaaccctt caagtggat 
      gatctcaaa gtgtctgtt ataaccggt caaggaaaa 
      agaagtcga gtgatggga atcatagga gtaacccga 
      ggttttggt gatcacgat attctttcc ctttatcaa 
      agaactccg ataaaaccg ttcctttct tcgcatcct 
      gaagtgcaa gttcataaa ataacgaac gttaccgaa 
      aatgaagtc ttagtgatg ggcactgat ggattatgg 
      gacgttgtg tgtggtgcg aaagcagca gaagtcgtt 
      gccaaatcg ctaaatact tttatggaa aaagaacgt 
      tacgttagt gcagctact tgtttagtt ggatcggcg 
      agagggtcc ctagtgaat gagcatagt tggcaactt 
      aaagacgga aaaccatcc tccgctgat gatatatca 
      gtatttgtt attccttta ttaccttac aaattagaa 
      tatgagaaa ttacttgga aagtatagc gaaaccgct 
      gaaagctca tag
    mRNA sequence
    • >TC016037 RA
      tgtcagcag atagcagat gtcacaaaa ataaaaatg 
      acagtttgt gatgtgtaa tcaagtaat cagtgattg 
      aaatgaaat aacgccgtt taaatgtga taaatccaa 
      tgactgctt tttaattgt gcgtcaaat atgttcagc 
      cgtttcaaa aatgcagtt ataggtgct gtgagtggt 
      ttagaaacg aacggcatc ccaaccccc aacgacccc 
      cattcctac aacaaacaa actttggcc ccaaagttt 
      ttatacggt cgacctgcg ttcttaaat ttgtctgaa 
      gatgaagtg caaatgtcg gccgaccac agactcagg 
      cctatcatt cacccaagc atgtctctg ccccatcat 
      gcaggatat gccgagtgt gttaacgca gggaagtcc 
      aaatggaac gaggatcag gcggtgtac aggcaaggt 
      gtcttgaca agggtagaa tataatgat aagcaaggt 
      gtgcaaaaa ttttccatc ccgtatact tattatggc 
      atttttgat ggccatgct ggagttggg gcagctctg 
      tgtgcggca aatcagctc caccatatt ttacatgaa 
      aagttggta gatgcccaa gatgacatt tggatagct 
      ttcagtgaa aaaaagagg ctgaaaacc aaacctagt 
      gacttactt ataacaggt gctttagaa gctgctttc 
      aaagaaatg gatcaagtt atagctgag gataggact 
      aaatatcaa gctgctggg ggttgtact gcgttagta 
      gcgctattt attttagat aaattattt gttgccaat 
      gcaggcgat tcacgcgca attatttgt aaagataat 
      aaatttaca ctaatgtca atggatttt actccagaa 
      aacgaacgt gacagaatt agaaggctt gcagaacaa 
      caaccaaat ttattaggg caggagttt acatcaaaa 
      gaatatgtt cggcctccg aaatcgagc gagcttggt 
      aaaacagta atgtatcga gacgctcat atgagtggt 
      tgggcctat aaaaccctt caagtggat gatctcaaa 
      gtgtctgtt ataaccggt caaggaaaa agaagtcga 
      gtgatggga atcatagga gtaacccga ggttttggt 
      gatcacgat attctttcc ctttatcaa agaactccg 
      ataaaaccg ttcctttct tcgcatcct gaagtgcaa 
      gttcataaa ataacgaac gttaccgaa aatgaagtc 
      ttagtgatg ggcactgat ggattatgg gacgttgtg 
      tgtggtgcg aaagcagca gaagtcgtt gccaaatcg 
      ctaaatact tttatggaa aaagaacgt tacgttagt 
      gcagctact tgtttagtt ggatcggcg agagggtcc 
      ctagtgaat gagcatagt tggcaactt aaagacgga 
      aaaccatcc tccgctgat gatatatca gtatttgtt 
      attccttta ttaccttac aaattagaa tatgagaaa 
      ttacttgga aagtatagc gaaaccgct gaaagctca 
      tagacacca cggagtttg tttgaattt atttttgtg 
      ttgcttctg gtctcaacg tcactccaa agattagtg 
      atacgagtc attattata tttgaagtt ttcatatct 
      tgttacatg tgtttgtac ctgtgcggt tttatatta 
      tgatttatg aaccaaggt cgcatttat gtatttgga 
      ccttatcca ttcattcct tatattttc gtaaagtta 
      tacccgatc gggaaacgt ttgcaatag aattgatac 
      aaaaagtat tttttct