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Gene information
General information
TC015986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid51: 4557..40988
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015986
    iBeetle screen iB_02541
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late rounded - Number of animals showing the phenotype: 2

    vitellogenic egg chamber partially degenerated - Number of animals showing the phenotype: 4

    Comment: ovaries look like potential held-eggs
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 3
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection



    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015986 RA
      MFKFIKAKG PHPSAERQK LQKDLFCYR RTLQHGFPH 
      KPTALAWDP SLRLLCIGT ATGAVKVFG RPGVEFYGH 
      HQNPIAVTQ AVFLPNQGR VVTLCDDNS LHLWEVNKS 
      SLVEVKSQA LEGKLKKIS TMCVETAGK HLLLGTEGG 
      NIYLLDLAS FTMTPDIIY QDVVMQNVP QDYKLNPGA 
      VEAIFEQPE HPDHILIGY NRGLIVLWN RSDNTSIKT 
      FVSNQQLEA LCWHDDGKT FTSSHNDGS YMVWDLDQN 
      KPMQDPVTT YGPFACRAI PKIIRRELR GKPLIVFSG 
      GLPRSSGSD KYTVSVIHD EQHVAFDFT SKVIDFIVI 
      DSKAEEQEG EENVVTNGT SSGEPDALV VLAEEELVV 
      IDISTKDWK MINLPYLVS LHASAVTCS QHVSGVPEE 
      LWEQLKEAG KAQTNNIYS SRSWPIDGG KLLCSKGSE 
      PKRELLLTG HEDGTVRFW DAGGVTLTP IYKFSTAMF 
      FTGDDLEEA VPDPEDVDD DWPPFRKIG VFDPYSDDP 
      RLAVKKVVL CPLSGSLIV AGTAGHVVV AKFDTEVLD 
      GEIKVTTMN IVSDRDGFV WKGHNQLAP KQGEIHQTR 
      GFQATSILQ LHPPAAVTA CVLQADWGL VAAGTAHGL 
      ALFDYVKNK PVMVKCTLN PNDLTGAGD TPISRRKSF 
      KKSLRESFR RLRKGRSTR RANPEITPA TNSPPNRKT 
      TQEDGQFSP LEAKPVERQ IEARPVDDS MSSFVRCLY 
      FARTFLVNV QNTNPTLWA GTNNGTVYT FTITVPSST 
      KRETDDVTC HIAKEIQLK HRAPVIAIA VLDGANRPL 
      PEPLEVEKG VAPLPDTTQ PHRVIIASE EQFKIFTLP 
      AVKAFCKFK LTAQDGARV RRMAFATFS CKNPDTDAT 
      HSEIDLLCL TNIGECLVF SIPDLKRQL NAAAIKRED 
      INGISSLVF TKSAEALYL HSSSELQRI SLSATCATT 
      ARCYLPQPV GEKEDAASD AGSEGERQE GQDVPPLVN 
      GNAEGSVGD KAEEVRENG EETPHNVTV SSSVGDITI 
      DSVKDYLGS GEELNRRIS GMCVTKTVT TVINSSQDG 
      AVTSSSTTT TTTSAQDNI IESDTDGPA PTLEIKKIQ 
      VSEILRAPL ALAEELDSD GQLNGDA
    • >TC015986 RB
      MFKFIKAKG PHPSAERQK LQKDLFCYR RTLQHGFPH 
      KPTALAWDP SLRLLCIGT ATGAVKVFG RPGVEFYGH 
      HQNPIAVTQ AVFLPNQGR VVTLCDDNS LHLWEVNKS 
      SLVEVKSQA LEGKLKKIS TMCVETAGK HLLLGTEGG 
      NIYLLDLAS FTMTPDIIY QDVVMQNVP QDYKLNPGA 
      VEAIFEQPE HPDHILIGY NRGLIVLWN RSDNTSIKT 
      FVSNQQLEA LCWHDDGKT FTSSHNDGS YMVWDLDQN 
      KPMQDPVTT YGPFACRAI PKIIRRELR GKPLIVFSG 
      GLPRSSGSD KYTVSVIHD EQHVAFDFT SKVIDFIVI 
      DSKAEEQEG EENVVTNGT SSGEPDALV VLAEEELVV 
      IDISTKDWK MINLPYLVS LHASAVTCS QHVSGVPEE 
      LWEQLKEAG KAQTNNIYS SRSWPIDGG KLLCSKGSE 
      PKRELLLTG HEDGTVRFW DAGGVTLTP IYKFSTAMF 
      FTGDDLEEA VPDPEDVDD DWPPFRKIG VFDPYSDDP 
      RLAVKKVVL CPLSGSLIV AGTAGHVVV AKFDTEVLD 
      GEIKVTTMN IVSDRDGFV WKGHNQLAP KQGEIHQTR 
      GFQATSILQ LHPPAAVTA CVLQADWGL VAAGTAHGL 
      ALFDYVKNK PVMVKCTLN PNDLTGAGD TPISRRKSF 
      KKSLRESFR RLRKGRSTR RANPEITPA TNSPPNRKT 
      TQEDGQFSP LEAKPVERQ IEARPVDDS MSSFVRCLY 
      FARTFLVNV QNTNPTLWA GTNNGTVYT FTITVPSST 
      KRETDDVTC HIAKEIQLK HRAPVIAIA VLDGANRPL 
      PEPLEVEKG VAPLPDTTQ PHRVIIASE EQFKIFTLP 
      AVKAFCKFK LTAQDGARV RRMAFATFS CKNPDTDAT 
      HSEIDLLCL TNIGECLVF SIPDLKRQL NAAAIKRED 
      INGISSLVF TKSAEALYL HSSSELQRI SLSATCATT 
      ARCYLPQPV GEKEDAASD AGSEGERQE GQDVPPLVN 
      GNAEGSVGD KAEEVRENG EETPHNVTV SSSVGDITI 
      DSVKDYLGS GEELNRRIS GMCVTKTVT TVINSSQDG 
      AVTSSSTTT TTTSAQDNN
    CDS sequence
    • >TC015986 RA
      atgttcaag tttattaag gccaaaggg ccgcatccc 
      agcgccgaa cgccagaag ctccaaaaa gaccttttc 
      tgttaccgg aggacgttg caacacgga ttcccccac 
      aaacccacc gcactagca tgggatccc tcattgcgc 
      cttctttgc attggaacg gccacagga gccgtcaag 
      gtcttcgga cgtccaggt gtcgaattt tatggtcac 
      catcagaac cccattgct gtaacccaa gccgtcttc 
      ctgccaaac caaggccgt gtggtcact ctatgtgac 
      gacaactcc ctccacctg tgggaggtg aacaaaagt 
      agtttggtt gaagtcaaa tcgcaagcc ctcgaaggc 
      aaattgaaa aaaatttcg accatgtgc gtcgaaact 
      gctggaaaa catctactt ctaggcaca gagggtggg 
      aatatttac cttttggat ttggcaagc tttacgatg 
      acacctgat ataatttat caggatgtg gtgatgcaa 
      aatgtgcct caggattac aaactgaat ccgggtgcc 
      gttgaggct atttttgaa caaccggaa catcccgat 
      catatactg attgggtac aatagagga ctgattgtt 
      ttgtggaat cgtagcgat aacacgtcc atcaaaact 
      tttgtttca aatcaacag ttggaggcg ctttgttgg 
      cacgatgat ggaaagaca ttcacaagc tcacataat 
      gatggcagc tatatggtt tgggatctg gaccaaaac 
      aagccaatg caagaccct gtcacaaca tacggccct 
      tttgcatgt agagccatt ccaaaaata ataagaaga 
      gaacttaga ggcaaacct ctgattgtt ttttccggt 
      ggtctacct cggtccagc ggcagtgac aaatacacc 
      gtttcggtt attcatgac gagcaacat gtcgctttt 
      gatttcaca agtaaggtg atagacttt atagtcatc 
      gattccaaa gcagaagag caagaaggt gaagagaat 
      gtcgtaaca aatggcaca agcagtggc gaacctgat 
      gctcttgtc gtattggcc gaagaagaa ttagtagtg 
      atcgatatt tcaacaaaa gattggaag atgataaat 
      ctaccgtat ttagtttcg ctacatgca tctgcagtt 
      acttgtagt caacacgtt tctggtgtt cccgaagag 
      ctttgggaa caactaaaa gaagcaggc aaagctcaa 
      accaacaac atttactct tcccgatcg tggcctatc 
      gacggtggc aaactccta tgttcgaaa ggttccgaa 
      ccgaagcga gaactttta ctaacgggt catgaagac 
      ggcactgtt cggttttgg gatgctggt ggtgtgact 
      ctcactccg atttacaaa tttagtaca gcgatgttt 
      ttcactggt gatgattta gaagaggct gttcctgat 
      cctgaagat gtggatgat gattggcca ccgtttcgt 
      aaaattgga gtttttgat ccgtattcg gatgaccca 
      cgattggct gttaagaaa gttgttttg tgtccgctt 
      tcgggaagt ttgattgtt gccggaact gctgggcat 
      gtggtggtc gctaaattt gatactgaa gtgttggat 
      ggtgaaatt aaagtcaca acgatgaat atcgtgagt 
      gatcgggac gggttcgtg tggaaggga cacaatcaa 
      ctagctcct aaacaaggg gaaatccac caaacacgt 
      ggtttccaa gctacttcc atcctccaa ttacatccc 
      cctgcagct gtcactgcc tgtgtcctt caagctgat 
      tggggttta gtcgctgct ggtacagcc cacggcctt 
      gcacttttc gactacgtt aaaaacaaa ccagtaatg 
      gtgaaatgc acactaaac cctaacgat ctcaccggt 
      gccggcgac acgcccatt tcccgtcgc aagtccttc 
      aaaaaatcc cttcgcgaa tcgttccga cgtctgcga 
      aaaggtcga tctacacgt cgagcaaac cccgaaatc 
      acacctgcc acaaactca ccccctaac cgcaaaaca 
      acccaagag gatggtcaa ttcagtccg ctcgaagcc 
      aaaccggtc gaaagacaa attgaagca cgccccgtt 
      gacgattcg atgagttcg tttgtaaga tgtctttat 
      ttcgcaagg acgttcctc gtaaatgta caaaacacg 
      aacccaact ttatgggct ggaaccaac aacggaacc 
      gtctatacc ttcacgata acggttcca tcttcgacg 
      aaacgcgaa acggacgac gtcacctgt cacatcgcc 
      aaggaaatt cagttaaag catcgagcg ccagttatt 
      gccattgcg gtgcttgat ggtgcaaac agacctctg 
      ccagaacca ctggaagtt gaaaagggg gtagcacca 
      cttccagat accacacaa cctcatcga gtgattatt 
      gcatcagag gaacagttt aagattttt actttaccg 
      gcggttaaa gcgttctgt aaatttaag ttgacggca 
      caagatggt gcaagagtg agacggatg gcttttgct 
      acgttcagt tgtaaaaat cccgatacg gatgcgaca 
      cattccgaa attgatttg ttgtgtctg acgaatatc 
      ggagagtgc cttgttttt agcattccg gatttgaag 
      agacagttg aatgccgcg gcgatcaag agggaggat 
      atcaatgga atttcgtcg ttggtcttc acgaaaagc 
      gccgaagca ctttatttg cattcctca tcggagctt 
      caaagaata tctttgtca gctacatgt gcaactact 
      gccagatgt tacctccct cagccagtg ggagagaaa 
      gaggacgct gcttctgat gccggttca gaaggtgaa 
      cggcaagag ggacaagac gtaccgccg ttagttaac 
      ggaaatgcc gaaggaagc gttggcgat aaggcggaa 
      gaagtgcga gaaaatggc gaagaaact ccccacaac 
      gtgacagtt tctagcagc gtcggcgat ataacaatc 
      gacagcgtt aaggattac ctcggctca ggtgaggag 
      cttaacaga cgcatttcc ggtatgtgt gtgacgaag 
      actgtcacg acagtcata aacagtagt caagacggt 
      gccgtaacg tcttccagt acgaccaca accacaaca 
      agtgcccaa gacaacatc atagagagt gacacggac 
      ggacctgca ccgacgtta gagattaag aaaatccaa 
      gttagcgaa attctccgc gccccacta gcattggcc 
      gaagaactg gattcggac ggtcaattg aacggagac 
      gcataa
    • >TC015986 RB
      atgttcaag tttattaag gccaaaggg ccgcatccc 
      agcgccgaa cgccagaag ctccaaaaa gaccttttc 
      tgttaccgg aggacgttg caacacgga ttcccccac 
      aaacccacc gcactagca tgggatccc tcattgcgc 
      cttctttgc attggaacg gccacagga gccgtcaag 
      gtcttcgga cgtccaggt gtcgaattt tatggtcac 
      catcagaac cccattgct gtaacccaa gccgtcttc 
      ctgccaaac caaggccgt gtggtcact ctatgtgac 
      gacaactcc ctccacctg tgggaggtg aacaaaagt 
      agtttggtt gaagtcaaa tcgcaagcc ctcgaaggc 
      aaattgaaa aaaatttcg accatgtgc gtcgaaact 
      gctggaaaa catctactt ctaggcaca gagggtggg 
      aatatttac cttttggat ttggcaagc tttacgatg 
      acacctgat ataatttat caggatgtg gtgatgcaa 
      aatgtgcct caggattac aaactgaat ccgggtgcc 
      gttgaggct atttttgaa caaccggaa catcccgat 
      catatactg attgggtac aatagagga ctgattgtt 
      ttgtggaat cgtagcgat aacacgtcc atcaaaact 
      tttgtttca aatcaacag ttggaggcg ctttgttgg 
      cacgatgat ggaaagaca ttcacaagc tcacataat 
      gatggcagc tatatggtt tgggatctg gaccaaaac 
      aagccaatg caagaccct gtcacaaca tacggccct 
      tttgcatgt agagccatt ccaaaaata ataagaaga 
      gaacttaga ggcaaacct ctgattgtt ttttccggt 
      ggtctacct cggtccagc ggcagtgac aaatacacc 
      gtttcggtt attcatgac gagcaacat gtcgctttt 
      gatttcaca agtaaggtg atagacttt atagtcatc 
      gattccaaa gcagaagag caagaaggt gaagagaat 
      gtcgtaaca aatggcaca agcagtggc gaacctgat 
      gctcttgtc gtattggcc gaagaagaa ttagtagtg 
      atcgatatt tcaacaaaa gattggaag atgataaat 
      ctaccgtat ttagtttcg ctacatgca tctgcagtt 
      acttgtagt caacacgtt tctggtgtt cccgaagag 
      ctttgggaa caactaaaa gaagcaggc aaagctcaa 
      accaacaac atttactct tcccgatcg tggcctatc 
      gacggtggc aaactccta tgttcgaaa ggttccgaa 
      ccgaagcga gaactttta ctaacgggt catgaagac 
      ggcactgtt cggttttgg gatgctggt ggtgtgact 
      ctcactccg atttacaaa tttagtaca gcgatgttt 
      ttcactggt gatgattta gaagaggct gttcctgat 
      cctgaagat gtggatgat gattggcca ccgtttcgt 
      aaaattgga gtttttgat ccgtattcg gatgaccca 
      cgattggct gttaagaaa gttgttttg tgtccgctt 
      tcgggaagt ttgattgtt gccggaact gctgggcat 
      gtggtggtc gctaaattt gatactgaa gtgttggat 
      ggtgaaatt aaagtcaca acgatgaat atcgtgagt 
      gatcgggac gggttcgtg tggaaggga cacaatcaa 
      ctagctcct aaacaaggg gaaatccac caaacacgt 
      ggtttccaa gctacttcc atcctccaa ttacatccc 
      cctgcagct gtcactgcc tgtgtcctt caagctgat 
      tggggttta gtcgctgct ggtacagcc cacggcctt 
      gcacttttc gactacgtt aaaaacaaa ccagtaatg 
      gtgaaatgc acactaaac cctaacgat ctcaccggt 
      gccggcgac acgcccatt tcccgtcgc aagtccttc 
      aaaaaatcc cttcgcgaa tcgttccga cgtctgcga 
      aaaggtcga tctacacgt cgagcaaac cccgaaatc 
      acacctgcc acaaactca ccccctaac cgcaaaaca 
      acccaagag gatggtcaa ttcagtccg ctcgaagcc 
      aaaccggtc gaaagacaa attgaagca cgccccgtt 
      gacgattcg atgagttcg tttgtaaga tgtctttat 
      ttcgcaagg acgttcctc gtaaatgta caaaacacg 
      aacccaact ttatgggct ggaaccaac aacggaacc 
      gtctatacc ttcacgata acggttcca tcttcgacg 
      aaacgcgaa acggacgac gtcacctgt cacatcgcc 
      aaggaaatt cagttaaag catcgagcg ccagttatt 
      gccattgcg gtgcttgat ggtgcaaac agacctctg 
      ccagaacca ctggaagtt gaaaagggg gtagcacca 
      cttccagat accacacaa cctcatcga gtgattatt 
      gcatcagag gaacagttt aagattttt actttaccg 
      gcggttaaa gcgttctgt aaatttaag ttgacggca 
      caagatggt gcaagagtg agacggatg gcttttgct 
      acgttcagt tgtaaaaat cccgatacg gatgcgaca 
      cattccgaa attgatttg ttgtgtctg acgaatatc 
      ggagagtgc cttgttttt agcattccg gatttgaag 
      agacagttg aatgccgcg gcgatcaag agggaggat 
      atcaatgga atttcgtcg ttggtcttc acgaaaagc 
      gccgaagca ctttatttg cattcctca tcggagctt 
      caaagaata tctttgtca gctacatgt gcaactact 
      gccagatgt tacctccct cagccagtg ggagagaaa 
      gaggacgct gcttctgat gccggttca gaaggtgaa 
      cggcaagag ggacaagac gtaccgccg ttagttaac 
      ggaaatgcc gaaggaagc gttggcgat aaggcggaa 
      gaagtgcga gaaaatggc gaagaaact ccccacaac 
      gtgacagtt tctagcagc gtcggcgat ataacaatc 
      gacagcgtt aaggattac ctcggctca ggtgaggag 
      cttaacaga cgcatttcc ggtatgtgt gtgacgaag 
      actgtcacg acagtcata aacagtagt caagacggt 
      gccgtaacg tcttccagt acgaccaca accacaaca 
      agtgcccaa gacaacaat taa
    mRNA sequence
    • >TC015986 RA
      tacttttca tattctgcc ggtcagtga cacaactac 
      gtgtaatgg tgcgttagc gttagtgca gaattagtg 
      catattcgt gcaattcat gaatataaa attccaagt 
      cgagcacaa attaacaga ataaggaga aatgttcaa 
      gtttattaa ggccaaagg gccgcatcc cagcgccga 
      acgccagaa gctccaaaa agacctttt ctgttaccg 
      gaggacgtt gcaacacgg attccccca caaacccac 
      cgcactagc atgggatcc ctcattgcg ccttctttg 
      cattggaac ggccacagg agccgtcaa ggtcttcgg 
      acgtccagg tgtcgaatt ttatggtca ccatcagaa 
      ccccattgc tgtaaccca agccgtctt cctgccaaa 
      ccaaggccg tgtggtcac tctatgtga cgacaactc 
      cctccacct gtgggaggt gaacaaaag tagtttggt 
      tgaagtcaa atcgcaagc cctcgaagg caaattgaa 
      aaaaatttc gaccatgtg cgtcgaaac tgctggaaa 
      acatctact tctaggcac agagggtgg gaatattta 
      ccttttgga tttggcaag ctttacgat gacacctga 
      tataattta tcaggatgt ggtgatgca aaatgtgcc 
      tcaggatta caaactgaa tccgggtgc cgttgaggc 
      tatttttga acaaccgga acatcccga tcatatact 
      gattgggta caatagagg actgattgt tttgtggaa 
      tcgtagcga taacacgtc catcaaaac ttttgtttc 
      aaatcaaca gttggaggc gctttgttg gcacgatga 
      tggaaagac attcacaag ctcacataa tgatggcag 
      ctatatggt ttgggatct ggaccaaaa caagccaat 
      gcaagaccc tgtcacaac atacggccc ttttgcatg 
      tagagccat tccaaaaat aataagaag agaacttag 
      aggcaaacc tctgattgt tttttccgg tggtctacc 
      tcggtccag cggcagtga caaatacac cgtttcggt 
      tattcatga cgagcaaca tgtcgcttt tgatttcac 
      aagtaaggt gatagactt tatagtcat cgattccaa 
      agcagaaga gcaagaagg tgaagagaa tgtcgtaac 
      aaatggcac aagcagtgg cgaacctga tgctcttgt 
      cgtattggc cgaagaaga attagtagt gatcgatat 
      ttcaacaaa agattggaa gatgataaa tctaccgta 
      tttagtttc gctacatgc atctgcagt tacttgtag 
      tcaacacgt ttctggtgt tcccgaaga gctttggga 
      acaactaaa agaagcagg caaagctca aaccaacaa 
      catttactc ttcccgatc gtggcctat cgacggtgg 
      caaactcct atgttcgaa aggttccga accgaagcg 
      agaactttt actaacggg tcatgaaga cggcactgt 
      tcggttttg ggatgctgg tggtgtgac tctcactcc 
      gatttacaa atttagtac agcgatgtt tttcactgg 
      tgatgattt agaagaggc tgttcctga tcctgaaga 
      tgtggatga tgattggcc accgtttcg taaaattgg 
      agtttttga tccgtattc ggatgaccc acgattggc 
      tgttaagaa agttgtttt gtgtccgct ttcgggaag 
      tttgattgt tgccggaac tgctgggca tgtggtggt 
      cgctaaatt tgatactga agtgttgga tggtgaaat 
      taaagtcac aacgatgaa tatcgtgag tgatcggga 
      cgggttcgt gtggaaggg acacaatca actagctcc 
      taaacaagg ggaaatcca ccaaacacg tggtttcca 
      agctacttc catcctcca attacatcc ccctgcagc 
      tgtcactgc ctgtgtcct tcaagctga ttggggttt 
      agtcgctgc tggtacagc ccacggcct tgcactttt 
      cgactacgt taaaaacaa accagtaat ggtgaaatg 
      cacactaaa ccctaacga tctcaccgg tgccggcga 
      cacgcccat ttcccgtcg caagtcctt caaaaaatc 
      ccttcgcga atcgttccg acgtctgcg aaaaggtcg 
      atctacacg tcgagcaaa ccccgaaat cacacctgc 
      cacaaactc accccctaa ccgcaaaac aacccaaga 
      ggatggtca attcagtcc gctcgaagc caaaccggt 
      cgaaagaca aattgaagc acgccccgt tgacgattc 
      gatgagttc gtttgtaag atgtcttta tttcgcaag 
      gacgttcct cgtaaatgt acaaaacac gaacccaac 
      tttatgggc tggaaccaa caacggaac cgtctatac 
      cttcacgat aacggttcc atcttcgac gaaacgcga 
      aacggacga cgtcacctg tcacatcgc caaggaaat 
      tcagttaaa gcatcgagc gccagttat tgccattgc 
      ggtgcttga tggtgcaaa cagacctct gccagaacc 
      actggaagt tgaaaaggg ggtagcacc acttccaga 
      taccacaca acctcatcg agtgattat tgcatcaga 
      ggaacagtt taagatttt tactttacc ggcggttaa 
      agcgttctg taaatttaa gttgacggc acaagatgg 
      tgcaagagt gagacggat ggcttttgc tacgttcag 
      ttgtaaaaa tcccgatac ggatgcgac acattccga 
      aattgattt gttgtgtct gacgaatat cggagagtg 
      ccttgtttt tagcattcc ggatttgaa gagacagtt 
      gaatgccgc ggcgatcaa gagggagga tatcaatgg 
      aatttcgtc gttggtctt cacgaaaag cgccgaagc 
      actttattt gcattcctc atcggagct tcaaagaat 
      atctttgtc agctacatg tgcaactac tgccagatg 
      ttacctccc tcagccagt gggagagaa agaggacgc 
      tgcttctga tgccggttc agaaggtga acggcaaga 
      gggacaaga cgtaccgcc gttagttaa cggaaatgc 
      cgaaggaag cgttggcga taaggcgga agaagtgcg 
      agaaaatgg cgaagaaac tccccacaa cgtgacagt 
      ttctagcag cgtcggcga tataacaat cgacagcgt 
      taaggatta cctcggctc aggtgagga gcttaacag 
      acgcatttc cggtatgtg tgtgacgaa gactgtcac 
      gacagtcat aaacagtag tcaagacgg tgccgtaac 
      gtcttccag tacgaccac aaccacaac aagtgccca 
      agacaacat catagagag tgacacgga cggacctgc 
      accgacgtt agagattaa gaaaatcca agttagcga 
      aattctccg cgccccact agcattggc cgaagaact 
      ggattcgga cggtcaatt gaacggaga cgcataatc 
      gatgaaaat aacaattgc catatcaac atgtccagt 
      atttcgttt gatttttct acgatcgat tcggatttt 
      attgcatga acggttggt gttttctta atttttaaa 
      ccaaaactt tttaattgt gtgttaagt atcgttact 
      tagtttaag taagtatta aaaattcca gtacttcgt 
      ttgggacca cgttgaaaa tttgtgtta aaaatcgcg 
      gaatactgc acttgtttt ttatatatt tataatcgt 
      aagataagt gttaatatc aaagttatt tacaatcaa 
      taacaattg atgttggcg tcaaattgt aaagtattt 
      aaaaggcgt gatttttta tattattat tgaaacagt 
      attcactgc tttgtacac gtttaagta acttattca 
      aaaggggga tttaaagtt aacaaatcg accatcgtc 
      ttatcaaaa attttacac gtttttata caaaatggt 
      ggaggttaa cgaatgtgt gtgtgagtg catccttta 
      tggccttaa catttcgtt ataatattt tatattatg 
      gtattttat tcacatttt attgaagat tatatttat 
      gacagaaac atcactgag tgaataaag tttttattg 
      gtacaagtg ttttactac cccttcgat tgaaaaacc 
      aaacaagta tttcagttt gtttattca aatcaacaa 
      atacaaacc aaatggaga ttgcgactt ttatttaca 
      cttaaaatt acaatatac aagatcaac aattatggc 
      acattataa taacatttt ttttagatc tgaattgag 
      gaaaaattc atttggctt ttttaacaa agtaataac 
      gctatttca acattaata aaaaaatct acagttagt 
      acaggatat gtcaccatt aagtttttg ttatttaat 
      tcttaaatc tattaatta ttttttata aaattgttt 
      taatacgtt tagtttagt gggtgcgtg gtgtaatca 
      atttaagca ccaaaaagg ctgaatatt ggataaaaa 
      attagttag tctatcaat taaaccacg cattgccgc 
      tttgatttt agatcgcta tattttatt aacaatttt 
      ttcacaaaa tgaagcgtt ccaggaagt attcagcta 
      cctattttt gtatgataa tccaaaatt caatgtgtt 
      taaaagaaa tgtggaaaa ggtaagtac ttgttttgt 
      tggtcgttg tgaaatgct cgccaaatt taagatttg 
      attgtcatt cgccaaacc ttaattaaa gtttaattc 
      agggtaagt ctcatttaa acttgcttg cattgcaat 
      taaaggttc aataaaccg accctataa caaattgta 
      attaaaaat attaaatag ttaagccaa attttgtga 
      tttctagag tgtgtttat tttgagata tagtaaaaa 
      attctttta gcagattat ctgtgtaat gataattta 
      atgagctta tttgaaaaa aatagttag gacacaata 
      atggtgaat gcgccgaga tagaattaa tttcttatc 
      aattttgtg tttagagta aacttaaaa acaggatag 
      atagtgaag acaaaacga atataaaaa acacaaaca 
      ttgatcttt ccatttttt ataacagta aaagtagta 
      ataagtgag agtggaaaa tttacgctt ggccgaaaa 
      ggtgtaagg aaaataagt ttattttca aaagtttaa 
      tccctaaaa actggaaaa atgggattt tagttgtta 
      ataaacagt ttaattcta a
    • >TC015986 RB
      tacttttca tattctgcc ggtcagtga cacaactac 
      gtgtaatgg tgcgttagc gttagtgca gaattagtg 
      catattcgt gcaattcat gaatataaa attccaagt 
      cgagcacaa attaacaga ataaggaga aatgttcaa 
      gtttattaa ggccaaagg gccgcatcc cagcgccga 
      acgccagaa gctccaaaa agacctttt ctgttaccg 
      gaggacgtt gcaacacgg attccccca caaacccac 
      cgcactagc atgggatcc ctcattgcg ccttctttg 
      cattggaac ggccacagg agccgtcaa ggtcttcgg 
      acgtccagg tgtcgaatt ttatggtca ccatcagaa 
      ccccattgc tgtaaccca agccgtctt cctgccaaa 
      ccaaggccg tgtggtcac tctatgtga cgacaactc 
      cctccacct gtgggaggt gaacaaaag tagtttggt 
      tgaagtcaa atcgcaagc cctcgaagg caaattgaa 
      aaaaatttc gaccatgtg cgtcgaaac tgctggaaa 
      acatctact tctaggcac agagggtgg gaatattta 
      ccttttgga tttggcaag ctttacgat gacacctga 
      tataattta tcaggatgt ggtgatgca aaatgtgcc 
      tcaggatta caaactgaa tccgggtgc cgttgaggc 
      tatttttga acaaccgga acatcccga tcatatact 
      gattgggta caatagagg actgattgt tttgtggaa 
      tcgtagcga taacacgtc catcaaaac ttttgtttc 
      aaatcaaca gttggaggc gctttgttg gcacgatga 
      tggaaagac attcacaag ctcacataa tgatggcag 
      ctatatggt ttgggatct ggaccaaaa caagccaat 
      gcaagaccc tgtcacaac atacggccc ttttgcatg 
      tagagccat tccaaaaat aataagaag agaacttag 
      aggcaaacc tctgattgt tttttccgg tggtctacc 
      tcggtccag cggcagtga caaatacac cgtttcggt 
      tattcatga cgagcaaca tgtcgcttt tgatttcac 
      aagtaaggt gatagactt tatagtcat cgattccaa 
      agcagaaga gcaagaagg tgaagagaa tgtcgtaac 
      aaatggcac aagcagtgg cgaacctga tgctcttgt 
      cgtattggc cgaagaaga attagtagt gatcgatat 
      ttcaacaaa agattggaa gatgataaa tctaccgta 
      tttagtttc gctacatgc atctgcagt tacttgtag 
      tcaacacgt ttctggtgt tcccgaaga gctttggga 
      acaactaaa agaagcagg caaagctca aaccaacaa 
      catttactc ttcccgatc gtggcctat cgacggtgg 
      caaactcct atgttcgaa aggttccga accgaagcg 
      agaactttt actaacggg tcatgaaga cggcactgt 
      tcggttttg ggatgctgg tggtgtgac tctcactcc 
      gatttacaa atttagtac agcgatgtt tttcactgg 
      tgatgattt agaagaggc tgttcctga tcctgaaga 
      tgtggatga tgattggcc accgtttcg taaaattgg 
      agtttttga tccgtattc ggatgaccc acgattggc 
      tgttaagaa agttgtttt gtgtccgct ttcgggaag 
      tttgattgt tgccggaac tgctgggca tgtggtggt 
      cgctaaatt tgatactga agtgttgga tggtgaaat 
      taaagtcac aacgatgaa tatcgtgag tgatcggga 
      cgggttcgt gtggaaggg acacaatca actagctcc 
      taaacaagg ggaaatcca ccaaacacg tggtttcca 
      agctacttc catcctcca attacatcc ccctgcagc 
      tgtcactgc ctgtgtcct tcaagctga ttggggttt 
      agtcgctgc tggtacagc ccacggcct tgcactttt 
      cgactacgt taaaaacaa accagtaat ggtgaaatg 
      cacactaaa ccctaacga tctcaccgg tgccggcga 
      cacgcccat ttcccgtcg caagtcctt caaaaaatc 
      ccttcgcga atcgttccg acgtctgcg aaaaggtcg 
      atctacacg tcgagcaaa ccccgaaat cacacctgc 
      cacaaactc accccctaa ccgcaaaac aacccaaga 
      ggatggtca attcagtcc gctcgaagc caaaccggt 
      cgaaagaca aattgaagc acgccccgt tgacgattc 
      gatgagttc gtttgtaag atgtcttta tttcgcaag 
      gacgttcct cgtaaatgt acaaaacac gaacccaac 
      tttatgggc tggaaccaa caacggaac cgtctatac 
      cttcacgat aacggttcc atcttcgac gaaacgcga 
      aacggacga cgtcacctg tcacatcgc caaggaaat 
      tcagttaaa gcatcgagc gccagttat tgccattgc 
      ggtgcttga tggtgcaaa cagacctct gccagaacc 
      actggaagt tgaaaaggg ggtagcacc acttccaga 
      taccacaca acctcatcg agtgattat tgcatcaga 
      ggaacagtt taagatttt tactttacc ggcggttaa 
      agcgttctg taaatttaa gttgacggc acaagatgg 
      tgcaagagt gagacggat ggcttttgc tacgttcag 
      ttgtaaaaa tcccgatac ggatgcgac acattccga 
      aattgattt gttgtgtct gacgaatat cggagagtg 
      ccttgtttt tagcattcc ggatttgaa gagacagtt 
      gaatgccgc ggcgatcaa gagggagga tatcaatgg 
      aatttcgtc gttggtctt cacgaaaag cgccgaagc 
      actttattt gcattcctc atcggagct tcaaagaat 
      atctttgtc agctacatg tgcaactac tgccagatg 
      ttacctccc tcagccagt gggagagaa agaggacgc 
      tgcttctga tgccggttc agaaggtga acggcaaga 
      gggacaaga cgtaccgcc gttagttaa cggaaatgc 
      cgaaggaag cgttggcga taaggcgga agaagtgcg 
      agaaaatgg cgaagaaac tccccacaa cgtgacagt 
      ttctagcag cgtcggcga tataacaat cgacagcgt 
      taaggatta cctcggctc aggtgagga gcttaacag 
      acgcatttc cggtatgtg tgtgacgaa gactgtcac 
      gacagtcat aaacagtag tcaagacgg tgccgtaac 
      gtcttccag tacgaccac aaccacaac aagtgccca 
      agacaacaa ttaagaaaa tccaagtta gcgaaattc 
      tccgcgccc cactagcat tggccgaag aactggatt 
      cggacggtc aattgaacg gagacgcat aatcgatga 
      aaataacaa ttgccatat caacatgtc cagtatttc 
      gtttgattt ttctacgat cgattcgga ttttattgc 
      atgaacggt tggtgtttt cttaatttt taaaccaaa 
      actttttaa ttgtgtgtt aagtatcgt tacttagtt 
      taagtaagt attaaaaat tccagtact tcgtttggg 
      accacgttg aaaatttgt gttaaaaat cgcggaata 
      ctgcacttg ttttttata tatttataa tcgtaagat 
      aagtgttaa tatcaaagt tatttacaa tcaataaca 
      attgatgtt ggcgtcaaa ttgtaaagt atttaaaag 
      gcgtgattt tttatatta ttattgaaa cagtattca 
      ctgctttgt acacgttta agtaactta ttcaaaagg 
      gggatttaa agttaacaa atcgaccat cgtcttatc 
      aaaaatttt acacgtttt tatacaaaa tggtggagg 
      ttaacgaat gtgtgtgtg agtgcatcc tttatggcc 
      ttaacattt cgttataat attttatat tatggtatt 
      ttattcaca ttttattga agattatat ttatgacag 
      aaacatcac tgagtgaat aaagttttt attggtaca 
      agtgtttta ctacccctt cgattgaaa aaccaaaca 
      agtatttca gtttgttta ttcaaatca acaaataca 
      aaccaaatg gagattgcg acttttatt tacacttaa 
      aattacaat atacaagat caacaatta tggcacatt 
      ataataaca tttttttta gatctgaat tgaggaaaa 
      attcatttg gctttttta acaaagtaa taacgctat 
      ttcaacatt aataaaaaa atctacagt tagtacagg 
      atatgtcac cattaagtt tttgttatt taattctta 
      aatctatta attattttt tataaaatt gttttaata 
      cgtttagtt tagtgggtg cgtggtgta atcaattta 
      agcaccaaa aaggctgaa tattggata aaaaattag 
      ttagtctat caattaaac cacgcattg ccgctttga 
      ttttagatc gctatattt tattaacaa ttttttcac 
      aaaatgaag cgttccagg aagtattca gctacctat 
      ttttgtatg ataatccaa aattcaatg tgtttaaaa 
      gaaatgtgg aaaaggtaa gtacttgtt ttgttggtc 
      gttgtgaaa tgctcgcca aatttaaga tttgattgt 
      cattcgcca aaccttaat taaagttta attcagggt 
      aagtctcat ttaaacttg cttgcattg caattaaag 
      gttcaataa accgaccct ataacaaat tgtaattaa 
      aaatattaa atagttaag ccaaatttt gtgatttct 
      agagtgtgt ttattttga gatatagta aaaaattct 
      tttagcaga ttatctgtg taatgataa tttaatgag 
      cttatttga aaaaaatag ttaggacac aataatggt 
      gaatgcgcc gagatagaa ttaatttct tatcaattt 
      tgtgtttag agtaaactt aaaaacagg atagatagt 
      gaagacaaa acgaatata aaaaacaca aacattgat 
      ctttccatt ttttataac agtaaaagt agtaataag 
      tgagagtgg aaaatttac gcttggccg aaaaggtgt 
      aaggaaaat aagtttatt ttcaaaagt ttaatccct 
      aaaaactgg aaaaatggg attttagtt gttaataaa 
      cagtttaat tctaa