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Gene information
General information
TC015981 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 114679..135288
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015981
    iBeetle screen iB_02540

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 37.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Adult morphology
    elytra slightly surface irregular - Number of animals showing the phenotype: 4

    Comment: slightly wrinkled

    intercoxal process T3 slightly shortened - Number of animals showing the phenotype: 2


    intercoxal process A3 position dorsalized - Number of animals showing the phenotype: 2


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015981 RA
      MSGGPPHHP HSRPAPPTN PAWNHLQVP NFFPSRQPQ 
      LAHMSNEHS LLHQPTWHT PTTEPMKTI PTSHIFSLE 
      QMLSERQFP RSGVDLSVT RNGSSQNGE ILTTTPISL 
      SVRDTNKIN SLPASALDI QPVVEVKNV KSISPKNSK 
      KRVDSILER LNPNLEKAN EESVSVIVQ ASSHDENSN 
      SSSLNVPTN DEVVSPYSN EDSLDSNKS RRKRKPSKT 
      VRVTKEELP DQSTVKVDD NEEVSEEKK IPERRRSSE 
      EEVPAKTRR KTSSESETI DNIAAMVQE SLKSNEVNH 
      DSKAISVSV IKTNENMQE SDSTTAPKK ATNTQFVEV 
      ENKLEEMFA GIEDTSDPL KTDNETKGD LSMATTSQD 
      NVTSTSNDL TVLKKSPKK GKRKQRRMQ SESTPKKKL 
      GKKAGKTAL KLTKTKKNS KIEAMKDVY AYDSGSNAS 
      SSKSRGPFI QIRGPRDSP LSVSVINTP TNDDDSDRK 
      PHKTKKFHD DSEYRHKVR SKGLHCSTL SNQYDAQTK 
      DTSWICAFC KRGPHATET SGPTLNTSI PAPGDLFGP 
      YFISGNSPE FERRLEDPF DKQFKSKKI ARTLDGNVK 
      VKKKRKHSM EEDAFLGIT ETGNGFEVW AHEDCIVWS 
      EGVYLVGPK IVGLEEAVW TSCNVACGK CHLKGANIC 
      CLKRGCLRV AHVCCARLT EWHFDEENY KTFCPEHRV 
      V
    CDS sequence
    • >TC015981 RA
      atgtcggga ggccctccc catcacccc cacagcagg 
      cctgcgcct ccaacaaac cccgcctgg aaccacctc 
      caagtaccg aactttttc ccaagtcgg caacctcag 
      ctcgctcac atgtcaaac gaacattcc cttctacat 
      caaccgact tggcacacg ccaactacc gaaccaatg 
      aaaaccatc ccgacttcg cacattttc agcctcgag 
      caaatgctc tccgaaaga cagtttccg cgatccggt 
      gtcgattta tcagtgact cgaaacggc agcagccaa 
      aacggcgaa atactcacc acaactccg attagttta 
      agtgtgcgc gatacgaac aaaatcaac agtttaccg 
      gcgagtgct ttggacatt caacccgtc gtcgaagtg 
      aaaaacgtc aaatcgatt agtccgaaa aattcgaaa 
      aaacgggtc gattcgatc ctcgaaaga ctcaatccg 
      aatctcgag aaggcgaac gaagagagt gttagtgtg 
      atcgtgcag gcgagttcg cacgacgaa aacagcaat 
      tcgagtagt ttaaacgta ccgaccaac gacgaggtt 
      gtctcacct tatagcaat gaagatagc ttggacagc 
      aataagtcg agacggaaa cgaaagcca agcaaaacg 
      gtccgtgtg acgaaagag gaacttcca gaccaaagc 
      actgtcaaa gtcgacgat aatgaagaa gtaagtgag 
      gagaagaaa atcccagaa cggcggaga tcgtccgag 
      gaagaagta ccagcgaaa actcgacgg aaaacttcc 
      tccgaatcg gagacaata gacaacata gcagcaatg 
      gttcaggaa agcttgaaa agcaacgaa gtgaaccat 
      gactctaaa gcaatctct gttagtgta attaaaact 
      aatgaaaat atgcaagaa agtgattcg acaacggcg 
      ccgaaaaaa gccaccaat acgcaattt gtcgaagtt 
      gaaaataaa ttagaagaa atgtttgcc gggattgaa 
      gatacgagt gatcctctc aagactgat aatgaaacg 
      aaaggggat ttatcgatg gctacgacg agtcaagat 
      aatgtcact tcaacatcc aacgatttg acggtgttg 
      aagaaatcc cccaaaaag ggtaaaagg aaacaaaga 
      cggatgcaa tctgagtca accccaaag aagaaactc 
      gggaagaaa gctggtaag actgcttta aaactgacc 
      aaaacaaag aaaaactca aagattgaa gcaatgaaa 
      gacgtgtac gcttacgat tcgggtagc aacgctagt 
      tcaagtaaa tcccgtggc cctttcatc caaatccgt 
      ggtcctcgt gattcgcct ctttccgtc agtgtgatc 
      aacacaccg acgaacgac gacgactcc gatcgaaaa 
      ccacacaaa acgaagaaa ttccacgac gacagcgaa 
      tacagacac aaggtacgt tccaaaggc ctccactgt 
      agcaccttg agcaaccaa tacgacgcc caaaccaaa 
      gacacctcc tggatttgc gccttctgc aaacggggg 
      cctcacgcg actgagacc tccggccca accctcaac 
      acctcaata ccggctcct ggcgacctc ttcggcccc 
      tacttcatc tcgggtaat tccccggag tttgagcgg 
      cggctggag gaccccttc gacaagcag ttcaagagt 
      aagaaaatc gcgcgaacg cttgacgga aacgtcaag 
      gtgaagaag aagcggaag cactcgatg gaagaggac 
      gcgtttctg ggaatcacc gaaacgggc aacgggttc 
      gaggtgtgg gcgcacgag gactgcatc gtgtggtcg 
      gagggggtt tacctagtg ggccccaaa attgtgggc 
      ttggaggag gccgtgtgg acgagttgt aacgtggcg 
      tgcgggaag tgccacctc aagggggcg aacatttgc 
      tgtttgaag aggggctgt ctgagagtg gcgcacgtg 
      tgctgtgcg cggttgacg gagtggcac tttgacgag 
      gagaattat aagactttt tgtcccgaa cacagggtt 
      gtgtga
    mRNA sequence
    • >TC015981 RA
      tagtgtcag tgactcgta ttgtgttta gttaattct 
      agttttatc tagttctgt ggtaatttt tttcgctca 
      attttgtga aaaccccag ttaataaaa tgggcaata 
      aactgtgta aacatgccg tctcgtcag gaattgcat 
      gatttgagc caccagacg tgaggtaat tataaatca 
      attaatcgc caaacggcg gaaattcgt ccgccaggt 
      agcgttgcc gttccaaaa atgtagaaa accgggtcc 
      gccattgtt cgaagtcgg ctgttttat ggccgtgcc 
      ttcgcccaa atcgttaaa attctcgcg tcactgttt 
      cattgggga gtgtttgga atgatctga gggcccccc 
      gggtcgagg cggccgtcc caaggccca gcacggagt 
      gccaaagtt cgacagtca acagtgtgt tatttccca 
      aacatcgat tcaaccttt cgaatttgt gcggttgtc 
      ttattatca cttgagggc ccgccgtaa aaggcccca 
      accgacgac gggatcgtt taaattggt ttttattag 
      ccgtaacag cgcgatttc gggccgttt gggcgccca 
      gaagacttc gcccggatg acagggtct gtaattttg 
      ttgcgaaac ctaactagt tgaagcact gagtgtccc 
      tcactctcg ctactatgc caccagatt gaccaattc 
      tctgtgcat caaccaaaa tgtcgggag gccctcccc 
      atcaccccc acagcaggc ctgcgcctc caacaaacc 
      ccgcctgga accacctcc aagtaccga actttttcc 
      caagtcggc aacctcagc tcgctcaca tgtcaaacg 
      aacattccc ttctacatc aaccgactt ggcacacgc 
      caactaccg aaccaatga aaaccatcc cgacttcgc 
      acattttca gcctcgagc aaatgctct ccgaaagac 
      agtttccgc gatccggtg tcgatttat cagtgactc 
      gaaacggca gcagccaaa acggcgaaa tactcacca 
      caactccga ttagtttaa gtgtgcgcg atacgaaca 
      aaatcaaca gtttaccgg cgagtgctt tggacattc 
      aacccgtcg tcgaagtga aaaacgtca aatcgatta 
      gtccgaaaa attcgaaaa aacgggtcg attcgatcc 
      tcgaaagac tcaatccga atctcgaga aggcgaacg 
      aagagagtg ttagtgtga tcgtgcagg cgagttcgc 
      acgacgaaa acagcaatt cgagtagtt taaacgtac 
      cgaccaacg acgaggttg tctcacctt atagcaatg 
      aagatagct tggacagca ataagtcga gacggaaac 
      gaaagccaa gcaaaacgg tccgtgtga cgaaagagg 
      aacttccag accaaagca ctgtcaaag tcgacgata 
      atgaagaag taagtgagg agaagaaaa tcccagaac 
      ggcggagat cgtccgagg aagaagtac cagcgaaaa 
      ctcgacgga aaacttcct ccgaatcgg agacaatag 
      acaacatag cagcaatgg ttcaggaaa gcttgaaaa 
      gcaacgaag tgaaccatg actctaaag caatctctg 
      ttagtgtaa ttaaaacta atgaaaata tgcaagaaa 
      gtgattcga caacggcgc cgaaaaaag ccaccaata 
      cgcaatttg tcgaagttg aaaataaat tagaagaaa 
      tgtttgccg ggattgaag atacgagtg atcctctca 
      agactgata atgaaacga aaggggatt tatcgatgg 
      ctacgacga gtcaagata atgtcactt caacatcca 
      acgatttga cggtgttga agaaatccc ccaaaaagg 
      gtaaaagga aacaaagac ggatgcaat ctgagtcaa 
      ccccaaaga agaaactcg ggaagaaag ctggtaaga 
      ctgctttaa aactgacca aaacaaaga aaaactcaa 
      agattgaag caatgaaag acgtgtacg cttacgatt 
      cgggtagca acgctagtt caagtaaat cccgtggcc 
      ctttcatcc aaatccgtg gtcctcgtg attcgcctc 
      tttccgtca gtgtgatca acacaccga cgaacgacg 
      acgactccg atcgaaaac cacacaaaa cgaagaaat 
      tccacgacg acagcgaat acagacaca aggtacgtt 
      ccaaaggcc tccactgta gcaccttga gcaaccaat 
      acgacgccc aaaccaaag acacctcct ggatttgcg 
      ccttctgca aacgggggc ctcacgcga ctgagacct 
      ccggcccaa ccctcaaca cctcaatac cggctcctg 
      gcgacctct tcggcccct acttcatct cgggtaatt 
      ccccggagt ttgagcggc ggctggagg accccttcg 
      acaagcagt tcaagagta agaaaatcg cgcgaacgc 
      ttgacggaa acgtcaagg tgaagaaga agcggaagc 
      actcgatgg aagaggacg cgtttctgg gaatcaccg 
      aaacgggca acgggttcg aggtgtggg cgcacgagg 
      actgcatcg tgtggtcgg agggggttt acctagtgg 
      gccccaaaa ttgtgggct tggaggagg ccgtgtgga 
      cgagttgta acgtggcgt gcgggaagt gccacctca 
      agggggcga acatttgct gtttgaaga ggggctgtc 
      tgagagtgg cgcacgtgt gctgtgcgc ggttgacgg 
      agtggcact ttgacgagg agaattata agacttttt 
      gtcccgaac acagggttg tgtgatttt tttattggg 
      tttgacgtg attttattg tgttgctat tttgtaaat 
      gattgtaag atattcttt ccatcagta ttgtgtttt 
      tttaattcg tgattgcaa aatagcatt gaggattac 
      ttaattcga tggatgttc catacggaa atgtttgat 
      ttgatggaa ttacgttta gtgaatagt gttgtttat 
      agaacaata gtataaatg aatgaaaaa aaatgtaaa 
      atatgtctt aaaatacgc ttatttttt ttataaaat 
      ggaaaattt taaaaatac tctcatttt taaatgtat 
      accttgtat catgttaat gataaccgt agtaattat 
      ataccgaaa atgtgtaca ttgtgtata tagcaaaat 
      ttatgtttt tgggcaaaa gtatttaat aaatttaat 
      taaaactt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY