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Gene information
General information
TC015979 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 154634..161088
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015979
    iBeetle screen iB_02539

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015979 RA
      MSNPSEALA TARNAFSTG ITKNAPFRL NQLKALLRL 
      YEENTPAII AALTEDLHK SKYESLISE VDYLKNDAL 
      NMIYNLSTW MEPEKPEKT LVNIMDQVI IHKEPYGVV 
      LIIGAWNYP LQLVFAPLA GAIAAGNCA LLKPSELAP 
      ASSKLIAEL VPKYLDNDC FKVIEGGVA ETSALLKLR 
      FDYIFFTGS THIGKIVSE AAAKFLTPV TLELGGKSP 
      VYVDNTVDI EIAVNRIMW GKCFNSGQS CIAPDYLLC 
      TKEVQEKFI ETTKKVITQ WYGEKVKQS PDYGRIIND 
      NHFKRIVKL LEGTKIVYG GEYDSNERY IAPTIVVDV 
      KPTDPIMQE EIFGPILPI ITIDNAVDA INFVNQGEK 
      PLALYVFSN KKTDIDLFI KNTSSGGVC INDTMMHFS 
      CESLPFGGV GNSGLGAYH GRYSFDTFT HKKGTLVKN 
      AGKLGEKLQ NARYPPYNE SKAKFLSTM LKKRPTIPM 
      TYMWHFISF GLGVVATLA AKCACNYME YKKHKNQF
    CDS sequence
    • >TC015979 RA
      atgtcgaac ccctccgag gctttagcc accgcacgc 
      aatgcattt tccaccgga atcacaaaa aacgcccca 
      ttccgcctc aaccaacta aaagcactt ttacgttta 
      tacgaagaa aacacccct gcgatcatc gcagctctg 
      actgaagac ctgcacaaa tccaaatac gaatccttg 
      atatcagaa gtcgattat ctcaaaaac gacgccctt 
      aacatgatt tacaacctc tctacttgg atggaacca 
      gaaaaaccc gaaaaaacc ctcgtcaac atcatggac 
      caggtcata atccacaaa gaaccctac ggtgtagtc 
      ctcataatc ggagcttgg aactacccc ctccagctc 
      gttttcgcg ccccttgct ggggccata gcagctggg 
      aattgcgct ctcttgaaa ccgtctgaa ctagcacca 
      gcttcgtca aaactaatc gcagaactg gtccccaaa 
      taccttgat aacgactgt tttaaggtt attgaaggc 
      ggggttgct gaaacctct gcacttttg aagctacgg 
      ttcgattat atttttttc accgggtcc acacatatt 
      ggcaaaatt gtgagcgaa gctgcagct aagttcctc 
      acacctgtg acgcttgaa cttggcggg aaaagtcca 
      gtttatgtt gacaatact gtcgatatt gaaattgca 
      gttaatcga atcatgtgg gggaaatgt tttaattcg 
      ggccaaagt tgcatcgct cctgactat cttctatgc 
      accaaagaa gtgcaagag aagtttatc gaaactacg 
      aagaaagtg ataacgcaa tggtacggt gaaaaagtc 
      aaacaatcg cccgattat gggcgaatt atcaacgac 
      aatcacttc aaacgcatc gtcaagttg ttggaagga 
      acaaaaatt gtctatggg ggagagtat gactcaaat 
      gagcgttac atcgcaccg acgattgta gtcgatgta 
      aaaccaaca gatcctata atgcaggag gagattttc 
      ggcccgatt ctgcctatt ataaccata gataacgca 
      gtcgatgcc attaacttc gtcaatcaa ggggaaaaa 
      cccttagct ttgtacgtg ttctcgaac aagaagact 
      gacattgat ttgttcatt aagaacacg tcgagtggc 
      ggcgtttgt attaatgat accatgatg cacttctcg 
      tgtgagagt ttgcccttt ggaggggtt ggtaatagt 
      gggctgggg gcctaccat ggcaggtac agctttgat 
      acgttcacg cacaagaag ggaacactt gttaagaat 
      gcaggcaag ttgggtgag aagttgcaa aatgcgagg 
      tatccaccc tataatgaa agtaaggcc aagtttctt 
      agtaccatg ttgaagaaa cggccaacg attccgatg 
      acgtacatg tggcatttt atcagtttt gggctgggg 
      gttgtggcc actttggcg gccaaatgt gcgtgtaac 
      tacatggag tataagaaa cataagaat cagttttga
    mRNA sequence
    • >TC015979 RA
      cgaagataa cggatgact tggcattaa cacaggcat 
      aaaactgtc gtaggtttt agttcagag cagtgtgtg 
      tgaggaagg tcaaaaacg catccgttt ttccagtgc 
      tgtatttac catcataaa gtaaattat cgccgatct 
      attttaact tcaaggtaa gatgttact tgtaaaagc 
      cggcgttaa tcgactttg ctgcatttc catagaata 
      tctgacgtc cattaggca aaaaaatta catcgtcag 
      tctaaggtc aacaagtct gtcggtcaa tttcaatgg 
      catttaatt tttacaaag tgtaagtag ttattgtta 
      ttgagcttc agttttgtg gtgtcgacc tggaaccag 
      aatcaaatt ctcaaaatg tcgaacccc tccgaggct 
      ttagccacc gcacgcaat gcattttcc accggaatc 
      acaaaaaac gccccattc cgcctcaac caactaaaa 
      gcactttta cgtttatac gaagaaaac acccctgcg 
      atcatcgca gctctgact gaagacctg cacaaatcc 
      aaatacgaa tccttgata tcagaagtc gattatctc 
      aaaaacgac gcccttaac atgatttac aacctctct 
      acttggatg gaaccagaa aaacccgaa aaaaccctc 
      gtcaacatc atggaccag gtcataatc cacaaagaa 
      ccctacggt gtagtcctc ataatcgga gcttggaac 
      taccccctc cagctcgtt ttcgcgccc cttgctggg 
      gccatagca gctgggaat tgcgctctc ttgaaaccg 
      tctgaacta gcaccagct tcgtcaaaa ctaatcgca 
      gaactggtc cccaaatac cttgataac gactgtttt 
      aaggttatt gaaggcggg gttgctgaa acctctgca 
      cttttgaag ctacggttc gattatatt tttttcacc 
      gggtccaca catattggc aaaattgtg agcgaagct 
      gcagctaag ttcctcaca cctgtgacg cttgaactt 
      ggcgggaaa agtccagtt tatgttgac aatactgtc 
      gatattgaa attgcagtt aatcgaatc atgtggggg 
      aaatgtttt aattcgggc caaagttgc atcgctcct 
      gactatctt ctatgcacc aaagaagtg caagagaag 
      tttatcgaa actacgaag aaagtgata acgcaatgg 
      tacggtgaa aaagtcaaa caatcgccc gattatggg 
      cgaattatc aacgacaat cacttcaaa cgcatcgtc 
      aagttgttg gaaggaaca aaaattgtc tatggggga 
      gagtatgac tcaaatgag cgttacatc gcaccgacg 
      attgtagtc gatgtaaaa ccaacagat cctataatg 
      caggaggag attttcggc ccgattctg cctattata 
      accatagat aacgcagtc gatgccatt aacttcgtc 
      aatcaaggg gaaaaaccc ttagctttg tacgtgttc 
      tcgaacaag aagactgac attgatttg ttcattaag 
      aacacgtcg agtggcggc gtttgtatt aatgatacc 
      atgatgcac ttctcgtgt gagagtttg ccctttgga 
      ggggttggt aatagtggg ctgggggcc taccatggc 
      aggtacagc tttgatacg ttcacgcac aagaaggga 
      acacttgtt aagaatgca ggcaagttg ggtgagaag 
      ttgcaaaat gcgaggtat ccaccctat aatgaaagt 
      aaggccaag tttcttagt accatgttg aagaaacgg 
      ccaacgatt ccgatgacg tacatgtgg cattttatc 
      agttttggg ctgggggtt gtggccact ttggcggcc 
      aaatgtgcg tgtaactac atggagtat aagaaacat 
      aagaatcag ttttgaggt ttttgtaat tactattga 
      gtttatatt gttgtttgt aatattatg tttataata 
      aaatttgtg gattact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY