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Gene information
General information
TC015948 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1946552..1950521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015948
    iBeetle screen iB_02534
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 6
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 60.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection



    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015948 RA
      MAATSTINC LLIILLIQI CTGEVIQRP ELKEDAEDE 
      DDELGNEHS ALPKNLETF QVNQNQITN DNVKRYKCH 
      LCDPPNCSN PTVCFDAIQ CWKSRVRET SGLETVTRG 
      CTKETDQLP FLCRTPSFS GQQKRQASG QFQVDCCTG 
      DFCNKGDFP ELPILFTKN MTMHSDSVD YALKMTAAI 
      LGPVVVIGT LGAIVLYLM RRIHQRRLC LESRLDPDT 
      YYASDDLLR ATAAGDSTL REYLENSLT SGSGSGLPL 
      LIQRTMAKQ ISLAECIGK GRYGEVWRG IWFGENVAV 
      KIFFSRDEA SWNRETEIY STILLRHEN ILGYIGSDM 
      TSRNSCTQL WLITHYHPL GSLYDHLNR TTLTHQQLL 
      RLCLSIANG LVHLHTEIF GTEGKPAIA HRDIKSKNI 
      LVKNNGTCC IADFGLAVT HQQATDQLD IGSNPKVGT 
      KRYMAPEVL DETIRMDYF DSFRRADIY ALGLVLWEL 
      CRRTVSNGI AEDYKPPFY DVVPNDPSF EDMRKVVCV 
      DQQRPSLLN RWASDPILS VMAKLMREC WHHNPNVRL 
      PALRIKKTI LKLCSGDNL INLDDSMEV CV
    CDS sequence
    • >TC015948 RA
      atggccgcc acaagtacc ataaattgt ttattaata 
      atattattg atacaaatc tgcacaggt gaagtgatc 
      caaaggcct gagctgaag gaagacgcc gaggatgag 
      gacgacgaa ctcggcaat gagcacagc gctttacct 
      aaaaacctg gagactttt caagtcaat caaaatcag 
      ataacaaac gacaatgtg aagcgctac aaatgccac 
      ctgtgtgac cccccgaat tgctccaac ccgactgtg 
      tgtttcgac gccatccag tgctggaag tcgcgcgtt 
      cgggaaacg tccgggcta gagactgtg actaggggc 
      tgtacgaaa gagacagac caattgccg ttcttatgc 
      cgtactccg tcctttagt gggcaacag aagcgacaa 
      gccagcggc cagttccag gtcgattgc tgcactggc 
      gatttttgc aacaagggg gatttcccc gaattgccg 
      attttgttt accaagaac atgacgatg cactctgac 
      tcagttgat tatgcgctc aaaatgacg gccgcgatt 
      ctgggaccg gtcgtggtt attggcacg ttgggtgcc 
      atcgttctt tacttaatg cgacgcatt caccagcgc 
      cgattatgc ctcgaaagt cgtctggat cctgatacg 
      tattacgct tctgacgat ttgttgcga gcgacagct 
      gccggcgac agcaccttg cgcgaatac cttgaaaat 
      tctctaaca tcggggagc ggttctggt ctccctctc 
      ctcatccag agaacaatg gcaaaacaa atttccctc 
      gctgaatgc atcggaaaa ggccgatac ggcgaagtc 
      tggcgaggc atttggttc ggggaaaat gtagccgtg 
      aaaatattt ttctcgcga gacgaggcc agttggaac 
      agggaaacg gaaatatac agtacaatt ttgttacgg 
      cacgagaat attttgggg tacattggt tcggacatg 
      acgtctcgt aattcctgc actcagcta tggttaatc 
      acccattac cacccattg ggcagtctt tacgaccat 
      ctaaatcgc accacactc acccatcaa cagctactg 
      cgtttgtgt ctttcgatt gccaatggg ttggtgcac 
      ctgcacaca gagatattt gggactgaa ggaaagcca 
      gccatcgcc caccgcgat attaaaagt aaaaatatt 
      ctagtaaaa aataatggg acttgttgt atagccgat 
      tttgggctt gcggtgacg catcaacaa gccactgac 
      cagttagat attggttcc aatcccaaa gttgggacg 
      aaacgttac atggcccca gaggtttta gacgaaacg 
      atacgaatg gattatttc gattcgttc cgtcgagct 
      gatatttac gccctaggg cttgtcctg tgggaatta 
      tgccgaagg acagtaagt aatgggata gcggaggat 
      tataaaccg cctttttac gacgttgtg cccaatgat 
      cccagtttt gaagacatg aggaaggtt gtgtgtgtg 
      gatcagcaa cgtccgagt ttacttaac agatgggct 
      agtgatccg atattgagc gttatggcc aaattaatg 
      cgagaatgt tggcatcac aaccccaac gtgcgatta 
      ccagcgttg cgaataaag aagacgata ttaaagcta 
      tgtagcggt gataattta attaatttg gatgatagt 
      atggaagtt tgtgtgtga
    mRNA sequence
    • >TC015948 RA
      aaacaaaac aaaaatccc gacttgtgt tgttattta 
      tacggcgta atatggccg ccacaagta ccataaatt 
      gtttattaa taatattat tgatacaaa tctgcacag 
      gtgaagtga tccaaaggc ctgagctga aggaagacg 
      ccgaggatg aggacgacg aactcggca atgagcaca 
      gcgctttac ctaaaaacc tggagactt ttcaagtca 
      atcaaaatc agataacaa acgacaatg tgaagcgct 
      acaaatgcc acctgtgtg accccccga attgctcca 
      acccgactg tgtgtttcg acgccatcc agtgctgga 
      agtcgcgcg ttcgggaaa cgtccgggc tagagactg 
      tgactaggg gctgtacga aagagacag accaattgc 
      cgttcttat gccgtactc cgtccttta gtgggcaac 
      agaagcgac aagccagcg gccagttcc aggtcgatt 
      gctgcactg gcgattttt gcaacaagg gggatttcc 
      ccgaattgc cgattttgt ttaccaaga acatgacga 
      tgcactctg actcagttg attatgcgc tcaaaatga 
      cggccgcga ttctgggac cggtcgtgg ttattggca 
      cgttgggtg ccatcgttc tttacttaa tgcgacgca 
      ttcaccagc gccgattat gcctcgaaa gtcgtctgg 
      atcctgata cgtattacg cttctgacg atttgttgc 
      gagcgacag ctgccggcg acagcacct tgcgcgaat 
      accttgaaa attctctaa catcgggga gcggttctg 
      gtctccctc tcctcatcc agagaacaa tggcaaaac 
      aaatttccc tcgctgaat gcatcggaa aaggccgat 
      acggcgaag tctggcgag gcatttggt tcggggaaa 
      atgtagccg tgaaaatat ttttctcgc gagacgagg 
      ccagttgga acagggaaa cggaaatat acagtacaa 
      ttttgttac ggcacgaga atattttgg ggtacattg 
      gttcggaca tgacgtctc gtaattcct gcactcagc 
      tatggttaa tcacccatt accacccat tgggcagtc 
      tttacgacc atctaaatc gcaccacac tcacccatc 
      aacagctac tgcgtttgt gtctttcga ttgccaatg 
      ggttggtgc acctgcaca cagagatat ttgggactg 
      aaggaaagc cagccatcg cccaccgcg atattaaaa 
      gtaaaaata ttctagtaa aaaataatg ggacttgtt 
      gtatagccg attttgggc ttgcggtga cgcatcaac 
      aagccactg accagttag atattggtt ccaatccca 
      aagttggga cgaaacgtt acatggccc cagaggttt 
      tagacgaaa cgatacgaa tggattatt tcgattcgt 
      tccgtcgag ctgatattt acgccctag ggcttgtcc 
      tgtgggaat tatgccgaa ggacagtaa gtaatggga 
      tagcggagg attataaac cgccttttt acgacgttg 
      tgcccaatg atcccagtt ttgaagaca tgaggaagg 
      ttgtgtgtg tggatcagc aacgtccga gtttactta 
      acagatggg ctagtgatc cgatattga gcgttatgg 
      ccaaattaa tgcgagaat gttggcatc acaacccca 
      acgtgcgat taccagcgt tgcgaataa agaagacga 
      tattaaagc tatgtagcg gtgataatt taattaatt 
      tggatgata gtatggaag tttgtgtgt gacaatctg 
      aaacgcatc tgttttaat gttatttta tagtcaagg 
      gctagtttt taatccgtg gcgatttta aattattgt 
      tgaacattt tatcatagg atattatcg taggactaa 
      tttttcttt aatacttct tctttattt aagtattat 
      aattcatgt gccaaattg attttgcgt ggaagtgtg 
      ggaggacac cagtattat gattttatg gttttactt 
      gacaccaaa aattcgaaa atgggtgga acaattttg 
      ttgaaagtg ccatttatt ttactctta ctttcttga 
      ttttaacgc aacacgtat ggtgacatt tttacatgc 
      tcaatttaa cttaaactc tttattttt tccccaaat 
      gttgccgta ggtgttgat ttggtatgg aaatcttat 
      attgttgta aatatattt caaatgttt atatcttga 
      cgtcatatc tgttcattg aaactgctg gaattaggc 
      ttttatatt ccactaatt ctatcagtt tttagtgtt 
      attttttaa taaattcta gtaacagat tcatatcaa 
      tttgtttat caattgatt ataaatatc cgagagctc 
      attcgaatc ataatcaat tttttattg catttttac 
      gttaagttg tcatcatta gtatttatt gttcgtttt 
      attcatttg tacatacac tcattgatg ttctgtatc 
      caagaataa cataagcaa tacatttag tttttattt