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Gene information
General information
TC015947 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1950624..1953651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015947
    iBeetle screen iB_02533
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    potentially confused/crosscontaminated with : iB # (comma separated) iB_02546 adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) iB_02546
    Pupal morphology
    potentially confused/crosscontaminated with : iB # (comma separated) iB_02546
    Adult morphology
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) iB_02546
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    potentially confused/crosscontaminated with : adults of..., iB # (comma separated) iB_02546
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015947 RA
      MDSGSFGSS LTKTQRLIL GSFVLILVD VIWVASSEL 
      TKFIYSNET FEKPFFCTY VKTSMFAIY LLGFLFWQP 
      WKDNCSRPA NYIHVDTEQ EDENYYNDT NTTNSRLSN 
      PVYVPVKTP ELDRNSGTE SDDSSVRSV RFNKLAEVR 
      HMSETDATE ALLARLSYQ ATLRAGEIA KRAAIKLPV 
      LRVAKISLI FCLLWFLAN YSYQVALAQ TEAAMVNVL 
      SSTSSFFTL VLAAIFPSN QNDKFTLSK FLAVLLSLL 
      GTTLVSFSD ISLESSVPL GAFLSLLSA FFYATYLVF 
      LKRKVDHED KIDIPLFFG FVGFFNLIL LWPCFFFLH 
      FSGLEVFEW PSRQQVLLL LLNGVLGTV ISEALWLWG 
      CFLTSSLMA TMSMSLTIP MTMLMDVFL KKIVYPSLF 
      YTGSIPMVV AFFAVTLLS HYDNWDPVL DVIRCAYLS 
      ICRRTRFMR FAEMPSEQT EALIGINSN EHEA
    CDS sequence
    • >TC015947 RA
      atggattct ggcagtttt ggctcgtct ttaaccaaa 
      acccaacgc ttgatttta ggttcgttc gtgcttatt 
      ttagtcgac gtgatttgg gttgcttcc tcggagctt 
      acaaagttt atttacagc aatgagacg ttcgaaaag 
      ccctttttt tgcacatat gtcaagacc tcaatgttc 
      gcaatctac ttgctgggc tttttattc tggcagccc 
      tggaaggac aattgctcg cgcccagcc aactacatc 
      cacgtggac acggaacaa gaagacgag aattattac 
      aatgatacc aacactacc aacagcaga ctgagcaac 
      cctgtctac gtcccggtg aaaaccccg gaactcgac 
      cggaattcg ggcactgaa agcgacgac tcctccgtt 
      aggtctgtc cgttttaat aagttggcc gaagtgcgg 
      cacatgtcg gagacggac gccacggag gcccttctg 
      gcgcggctg tcctaccag gccactctg agggccggg 
      gaaattgcc aaaagggcc gctattaag cttccagtg 
      ttgcgagtg gcgaaaatt tcgctcatt ttttgcctt 
      ttgtggttt ctagccaac tacagctac caagtagct 
      ttagctcaa actgaggcg gcaatggtc aatgtcctg 
      tcgtcaact tccagcttt tttactcta gttttagcc 
      gctattttt ccttccaat cagaatgat aagttcacg 
      ttgtccaag tttcttgcc gtgttgctc agtctctta 
      ggaaccact ttggtgagc ttttcggac atttcgctc 
      gaatctagc gttccattg ggcgctttc ctctccctc 
      ttgagcgca tttttctac gccacttat ttagttttc 
      ctcaagcgc aaagtggat catgaggac aaaatcgac 
      attcctttg tttttcggt ttcgtggga tttttcaat 
      ttgattctc ctgtggcca tgtttcttc tttttgcac 
      tttagcgga ctggaagtt ttcgaatgg ccgtcgagg 
      caacaagtg ctgttgttg cttctgaat ggggtctta 
      gggaccgtt atctcggag gctttatgg ttatggggt 
      tgttttttg acgtcctcg ctgatggcg accatgtca 
      atgagtttg actattccg atgaccatg ttgatggat 
      gtttttttg aagaagatc gtctatccg tctctgttt 
      tacacaggg agtattcct atggtggtg gcgtttttt 
      gccgtcact ttactttcg cattacgac aattgggac 
      cctgtactc gacgttata cgctgtgcc tatctaagt 
      atttgccga cgaaccaga tttatgcgg tttgctgaa 
      atgccgtcg gaacaaacc gaagccttg attggtatt 
      aacagtaat gaacatgaa gcgtag
    mRNA sequence
    • >TC015947 RA
      aatctgtca aaaaaatcg taaacagaa tctgcattt 
      ttgcggcta attatgata acgtagcga ttgtgcaag 
      tagccgaag aactgataa ttttcgtcg ccggacgtc 
      cgtgtaaaa atgtcgccc acagacgta accactgat 
      gggttttta gcgcctttg ttatcccat tagaacact 
      atggttttt ggcacaaaa aacgcatta atgggcacg 
      aggaaatgg attctggca gttttggct cgtctttaa 
      ccaaaaccc aacgcttga ttttaggtt cgttcgtgc 
      ttattttag tcgacgtga tttgggttg cttcctcgg 
      agcttacaa agtttattt acagcaatg agacgttcg 
      aaaagccct ttttttgca catatgtca agacctcaa 
      tgttcgcaa tctacttgc tgggctttt tattctggc 
      agccctgga aggacaatt gctcgcgcc cagccaact 
      acatccacg tggacacgg aacaagaag acgagaatt 
      attacaatg ataccaaca ctaccaaca gcagactga 
      gcaaccctg tctacgtcc cggtgaaaa ccccggaac 
      tcgaccgga attcgggca ctgaaagcg acgactcct 
      ccgttaggt ctgtccgtt ttaataagt tggccgaag 
      tgcggcaca tgtcggaga cggacgcca cggaggccc 
      ttctggcgc ggctgtcct accaggcca ctctgaggg 
      ccggggaaa ttgccaaaa gggccgcta ttaagcttc 
      cagtgttgc gagtggcga aaatttcgc tcatttttt 
      gccttttgt ggtttctag ccaactaca gctaccaag 
      tagctttag ctcaaactg aggcggcaa tggtcaatg 
      tcctgtcgt caacttcca gctttttta ctctagttt 
      tagccgcta tttttcctt ccaatcaga atgataagt 
      tcacgttgt ccaagtttc ttgccgtgt tgctcagtc 
      tcttaggaa ccactttgg tgagctttt cggacattt 
      cgctcgaat ctagcgttc cattgggcg ctttcctct 
      ccctcttga gcgcatttt tctacgcca cttatttag 
      ttttcctca agcgcaaag tggatcatg aggacaaaa 
      tcgacattc ctttgtttt tcggtttcg tgggatttt 
      tcaatttga ttctcctgt ggccatgtt tcttctttt 
      tgcacttta gcggactgg aagttttcg aatggccgt 
      cgaggcaac aagtgctgt tgttgcttc tgaatgggg 
      tcttaggga ccgttatct cggaggctt tatggttat 
      ggggttgtt ttttgacgt cctcgctga tggcgacca 
      tgtcaatga gtttgacta ttccgatga ccatgttga 
      tggatgttt ttttgaaga agatcgtct atccgtctc 
      tgttttaca cagggagta ttcctatgg tggtggcgt 
      tttttgccg tcactttac tttcgcatt acgacaatt 
      gggaccctg tactcgacg ttatacgct gtgcctatc 
      taagtattt gccgacgaa ccagattta tgcggtttg 
      ctgaaatgc cgtcggaac aaaccgaag ccttgattg 
      gtattaaca gtaatgaac atgaagcgt aggtttagt 
      tgatttttt cttgctgtt tttatacct actatggtg 
      cttacaatt tatgtgatg ataagattt ttactttgt 
      gatcttttt tatttttat taattcatt gtaaatatg 
      cggagttaa ggaagtgta ttaaacgca cgtaatacg 
      ttcaaaaca gtaactaga caatatgtt ctacaatta 
      attagtagt attgtaact cgttcaact agcatttca 
      cgatctcat aggtttttc acaactttc gataagaaa 
      aagtctgtg tatgcattc catagttct aagccatat 
      atttttaat ataatgtgg aaacatgtg taacatatt 
      ttctgacaa aaacgtgtt agaatgtaa atattattg 
      cagaataga gtatattat gaacgcacc ttgagtgtt 
      tcaatctcc cgccttcct ccttgtttc ctaaagcac 
      gtaacgccg aaaacgttc cggccctca ttaatgctt 
      acgtgcccc cagttgtgg aaataaatt acacaaggc 
      cct