Status
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Gene information
General information
TC015935 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4156508..4160758
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015935
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02531

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality early (L5/6) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015935 RA
      MEISDENLR ILSQYLQQT LSPDPNTRR PAEKFLEGV 
      EVNQNYPLL LLNLVHKPE VDVTIRIAG AIAFKNYIK 
      RNWSIEEDQ NDRIHESDR LAIKNLIVS LMLSSPEAI 
      QKQLSDAIS IIGKTDFPL KWPELITEM VDKFATGDF 
      HVINGVLQT AHSLFKKYR YEFKSNELW MEIKYVLDK 
      LAKPLTDLL MATMGLTQA HQNDVSALK IIYHSLVLI 
      CKVFYSLNY QDLPEFFED NMATWMSNF HTLLTVEVK 
      VLQNSSQDE AGVIEQLKS QACDNIALY AQKYDEEFQ 
      PYLPQFVTA VWNLLVVTG LEPKYDLLV SNALQFLSS 
      VAERSHYRN LFEDNAVLS SICEQVIIP NMEFRASDG 
      ELFEDNPEE YIRRDIEGS DVDTRRRAA CDLVNTLSQ 
      NFEKRIIEI FGQYLQVML SKYNENPKD NWRSKDAAL 
      YLVTSLVSR GATQKHGVT QTSQLVSIP QFCQDQILP 
      ELKRTDVDE LPVLKADAI KYLLTFRSV LPSEMVVAT 
      IPLLIQHLT SESAVIHTY AACTIEKIL IMKDGNNQN 
      ILNGASLEP LASQLLVNL FAILDKPVS EENEYVMKT 
      VMRTFSTLQ DRVIPYLQS ALPKLTEKL QMVAKNPSK 
      PHFNHYLFE TISLAIKIV CKTNASAVT SFEDILFPI 
      FQGILQQDI QEFIPYVFQ ILSLLMEFT PIGSISDAY 
      IQLLPCLLA PVLWERPAN ISPLVRLLS AFTVHAGHQ 
      IVAQDKVGG FLGVFQKLI ASKSNDHEG FHLMQNMIQ 
      HFPIEALAP YHKQIFFLL FQRLSSSKT TKYVINIIV 
      FFCMYAVKY SPVELVSVI DGIQAQMFG MVLEKLLIS 
      ELQKVSGNV ERKIVACGI TKLLCECPE MLTGTYQKF 
      WPQLLQALI MFFEMPLDE SSLPDDHFI EIDDTPTFQ 
      TTSAKLNFA NIAKPDPLQ SFGEPRQFL SQNLATLGR 
      AQPGKLPVL VGNMNQQAQ TILNQYLAK FGVQLM
    CDS sequence
    • >TC015935 RA
      atggagatt tccgacgag aatcttcga attctgagc 
      caatatttg caacagact ttgagcccc gacccgaac 
      actcggcgc cccgccgaa aagttcctc gaaggggtc 
      gaagtgaac cagaattac cccttgttg ctgcttaat 
      ttagtgcac aaacccgag gtggatgtg acaatacgg 
      attgcaggc gcaattgcc ttcaaaaat tacatcaaa 
      cggaactgg agtattgag gaggaccag aatgatcgg 
      atccacgaa agcgatagg ttggctatt aagaatctc 
      attgtttct ctaatgttg agttcgcct gaagcaata 
      caaaaacag ttgagtgat gctattagc attatcgga 
      aagacagat tttccgttg aaatggccc gagttgatc 
      actgaaatg gtggacaaa tttgccaca ggcgatttc 
      cacgtcatt aatggggtt ctgcaaacg gcgcattct 
      ctctttaaa aaatatcgt tacgaattc aagtccaac 
      gaactctgg atggaaatt aaatacgta ctggacaag 
      ttagccaag cctttaact gatctcctt atggccact 
      atggggcta acacaagca caccaaaac gatgtcagt 
      gctttgaaa attatatac cattcactt gtcttaatt 
      tgtaaagtt ttctactct ttaaattac caagatctc 
      cccgaattt tttgaagac aacatggcc acttggatg 
      tcgaatttc cacacgctg ctaacagtt gaagttaaa 
      gtactgcaa aattcgagt caggacgag gctggcgta 
      atagaacag ttgaagtca caagcctgt gataacatt 
      gctttgtat gctcagaaa tatgatgag gaatttcaa 
      ccgtattta ccacaattt gtcacagct gtttggaat 
      ttgttggtt gttacaggc ctggagccg aaatatgat 
      ttgttggtt tccaacgct ctccaattt ttgtcgtca 
      gttgctgaa cgttctcat tacaggaat ttatttgag 
      gataatgcg gttttgagc agcatttgt gaacaagtt 
      atcattcca aatatggaa tttagagcc tcggatgga 
      gagttattt gaagacaat ccagaggaa tacattagg 
      agagacatt gaaggatct gatgtggac acaagacgg 
      cgagccgct tgtgatcta gtcaacact ttgagccag 
      aatttcgaa aaacgcatc atcgaaata ttcggacaa 
      tatttacag gtaatgcta tcaaaatac aacgaaaac 
      cccaaagac aactggcgc agcaaagac gccgccctt 
      tacttggta acatctctg gtaagtagg ggggccaca 
      caaaaacac ggtgtgact caaacgagc caacttgtc 
      tcaatacca caattctgt caagaccaa atcctccct 
      gagcttaaa agaaccgac gtcgacgaa ttaccggtc 
      cttaaggcc gatgcaatc aaatatttg ctaactttt 
      cgctctgtc ttacccagt gaaatggtc gttgcaacc 
      attcctttg ctaattcaa cacttgacg agcgaaagt 
      gcagtcatt cacacttat gctgcttgt actattgaa 
      aaaatccta atcatgaaa gacggcaac aatcagaat 
      attttaaac ggggcctct ttggagccc ttagccagt 
      cagctgctg gttaatctt ttcgcgatt ttggacaaa 
      ccggtgtcg gaggagaac gaatacgtt atgaaaacc 
      gtaatgagg actttttcc acgttgcaa gaccgagtc 
      attccgtat ttgcaatca gctttgccc aaactcacc 
      gagaagctg caaatggtt gcgaaaaat ccatccaag 
      cctcatttt aatcattat ttatttgaa accatttcc 
      ctggcgatt aagattgtg tgtaaaacg aacgcgtca 
      gcggttacg tcgtttgag gatattttg tttccgatt 
      tttcaaggc atattgcag caggatatt caagagttc 
      attccgtac gtgtttcaa attttgtcc cttttgatg 
      gaattcacc ccaattggg agcatatcc gacgcctac 
      atccagctt ttgccgtgt cttttggcc cccgttttg 
      tgggagcgc ccggccaac atcagcccc cttgttcgc 
      ctcctaagc gcctttacg gtgcatgcg ggccaccaa 
      atcgtggca caagataaa gtgggcggt tttttgggt 
      gttttccaa aaactgatc gcctcgaaa tcaaacgat 
      cacgaaggc ttccacttg atgcaaaac atgatccag 
      cattttcca atagaggcg ctcgctccg taccacaag 
      caaattttc ttcctgctt ttccagaga ctgtcgtcg 
      agcaaaacc accaaatat gttataaat ataattgtt 
      tttttctgt atgtatgcc gttaagtat tcgccggtt 
      gagctggtg tctgttatc gatggaatc caagcccag 
      atgtttggt atggttcta gagaagcta ctaatctcc 
      gaattgcaa aaagtctca ggcaacgtt gagaggaaa 
      attgtcgca tgtggcatc actaaattg ttatgtgaa 
      tgccccgaa atgctcact gggacgtat cagaagttc 
      tggccgcaa cttttacaa gctttaatc atgtttttt 
      gaaatgccg ctagacgaa agttcttta ccagacgac 
      cactttatc gagattgac gacaccccc acgtttcag 
      accactagt gccaagttg aattttgca aatattgcc 
      aaaccggac ccgttacaa agtttcggg gaaccgagg 
      cagtttttg tcgcaaaat ttggcaact cttggtcgt 
      gcacagcct ggaaaattg ccagttttg gtggggaat 
      atgaaccag caagcccag accatcctt aaccagtat 
      ctggcgaaa tttggagtc cagctcatg taa
    mRNA sequence
    • >TC015935 RA
      tgaggttaa atttatctg gggttatat caacggtcg 
      ttgaaaaaa aaagtgcgt tttttaaat tatttcaag 
      cgaaatgga gatttccga cgagaatct tcgaattct 
      gagccaata tttgcaaca gactttgag ccccgaccc 
      gaacactcg gcgccccgc cgaaaagtt cctcgaagg 
      ggtcgaagt gaaccagaa ttacccctt gttgctgct 
      taatttagt gcacaaacc cgaggtgga tgtgacaat 
      acggattgc aggcgcaat tgccttcaa aaattacat 
      caaacggaa ctggagtat tgaggagga ccagaatga 
      tcggatcca cgaaagcga taggttggc tattaagaa 
      tctcattgt ttctctaat gttgagttc gcctgaagc 
      aatacaaaa acagttgag tgatgctat tagcattat 
      cggaaagac agattttcc gttgaaatg gcccgagtt 
      gatcactga aatggtgga caaatttgc cacaggcga 
      tttccacgt cattaatgg ggttctgca aacggcgca 
      ttctctctt taaaaaata tcgttacga attcaagtc 
      caacgaact ctggatgga aattaaata cgtactgga 
      caagttagc caagccttt aactgatct ccttatggc 
      cactatggg gctaacaca agcacacca aaacgatgt 
      cagtgcttt gaaaattat ataccattc acttgtctt 
      aatttgtaa agttttcta ctctttaaa ttaccaaga 
      tctccccga attttttga agacaacat ggccacttg 
      gatgtcgaa tttccacac gctgctaac agttgaagt 
      taaagtact gcaaaattc gagtcagga cgaggctgg 
      cgtaataga acagttgaa gtcacaagc ctgtgataa 
      cattgcttt gtatgctca gaaatatga tgaggaatt 
      tcaaccgta tttaccaca atttgtcac agctgtttg 
      gaatttgtt ggttgttac aggcctgga gccgaaata 
      tgatttgtt ggtttccaa cgctctcca atttttgtc 
      gtcagttgc tgaacgttc tcattacag gaatttatt 
      tgaggataa tgcggtttt gagcagcat ttgtgaaca 
      agttatcat tccaaatat ggaatttag agcctcgga 
      tggagagtt atttgaaga caatccaga ggaatacat 
      taggagaga cattgaagg atctgatgt ggacacaag 
      acggcgagc cgcttgtga tctagtcaa cactttgag 
      ccagaattt cgaaaaacg catcatcga aatattcgg 
      acaatattt acaggtaat gctatcaaa atacaacga 
      aaaccccaa agacaactg gcgcagcaa agacgccgc 
      cctttactt ggtaacatc tctggtaag taggggggc 
      cacacaaaa acacggtgt gactcaaac gagccaact 
      tgtctcaat accacaatt ctgtcaaga ccaaatcct 
      ccctgagct taaaagaac cgacgtcga cgaattacc 
      ggtccttaa ggccgatgc aatcaaata tttgctaac 
      ttttcgctc tgtcttacc cagtgaaat ggtcgttgc 
      aaccattcc tttgctaat tcaacactt gacgagcga 
      aagtgcagt cattcacac ttatgctgc ttgtactat 
      tgaaaaaat cctaatcat gaaagacgg caacaatca 
      gaatatttt aaacggggc ctctttgga gcccttagc 
      cagtcagct gctggttaa tcttttcgc gattttgga 
      caaaccggt gtcggagga gaacgaata cgttatgaa 
      aaccgtaat gaggacttt ttccacgtt gcaagaccg 
      agtcattcc gtatttgca atcagcttt gcccaaact 
      caccgagaa gctgcaaat ggttgcgaa aaatccatc 
      caagcctca ttttaatca ttatttatt tgaaaccat 
      ttccctggc gattaagat tgtgtgtaa aacgaacgc 
      gtcagcggt tacgtcgtt tgaggatat tttgtttcc 
      gatttttca aggcatatt gcagcagga tattcaaga 
      gttcattcc gtacgtgtt tcaaatttt gtccctttt 
      gatggaatt caccccaat tgggagcat atccgacgc 
      ctacatcca gcttttgcc gtgtctttt ggcccccgt 
      tttgtggga gcgcccggc caacatcag cccccttgt 
      tcgcctcct aagcgcctt tacggtgca tgcgggcca 
      ccaaatcgt ggcacaaga taaagtggg cggtttttt 
      gggtgtttt ccaaaaact gatcgcctc gaaatcaaa 
      cgatcacga aggcttcca cttgatgca aaacatgat 
      ccagcattt tccaataga ggcgctcgc tccgtacca 
      caagcaaat tttcttcct gcttttcca gagactgtc 
      gtcgagcaa aaccaccaa atatgttat aaatataat 
      tgttttttt ctgtatgta tgccgttaa gtattcgcc 
      ggttgagct ggtgtctgt tatcgatgg aatccaagc 
      ccagatgtt tggtatggt tctagagaa gctactaat 
      ctccgaatt gcaaaaagt ctcaggcaa cgttgagag 
      gaaaattgt cgcatgtgg catcactaa attgttatg 
      tgaatgccc cgaaatgct cactgggac gtatcagaa 
      gttctggcc gcaactttt acaagcttt aatcatgtt 
      ttttgaaat gccgctaga cgaaagttc tttaccaga 
      cgaccactt tatcgagat tgacgacac ccccacgtt 
      tcagaccac tagtgccaa gttgaattt tgcaaatat 
      tgccaaacc ggacccgtt acaaagttt cggggaacc 
      gaggcagtt tttgtcgca aaatttggc aactcttgg 
      tcgtgcaca gcctggaaa attgccagt tttggtggg 
      gaatatgaa ccagcaagc ccagaccat ccttaacca 
      gtatctggc gaaatttgg agtccagct catgtaatt 
      tgttttgtt gtgttaact gaatgcttt cgaataaaa 
      cgatatttt taaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY