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Gene information
General information
TC015923 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 2144784..2155764
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015923
    iBeetle screen iB_02530
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    beetle stock: bad (increased lethality), adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015923 RA
      MSVLTTTLN NGLKMPMIG YGTWQAKDE ELEQAFEAA 
      LEAGYRHID TAHVYENEK VIGKVLNRW LSSGKLTRK 
      DIFLVTKLP PGGNRPEAV SKYIKRSLD LLQVQYLDL 
      FLIHVPFAF KDVEGDLHP MTPDGRIDI DPSTDHVAL 
      WKAMEAQLD AGLTKSIGL SNFNQKQIE RVLQNCRIP 
      PSNLQVELH AYLQQKELV EFCKTNKIV VTAYSPLGS 
      PGLAKFMAQ FGQKIDLPD ILNNPVVKA IAAKHKRSE 
      AQIVLRHAV QKGIVVIPK STNPKRLRE NIDIFGFEL 
      DQGDMSQLN GLDQGIRIL NFAAFKGIE EHPEYPFK
    CDS sequence
    • >TC015923 RA
      atgagtgtg cttacgact actttaaac aacggattg 
      aaaatgcca atgataggg tatggaacc tggcaagca 
      aaagacgaa gaactggag caagccttc gaagccgcc 
      ctggaagcc ggataccgc catatcgac acagctcac 
      gtctatgaa aatgaaaaa gttatcggc aaagttcta 
      aaccggtgg ctctcctcg ggcaaacta acccgcaaa 
      gacatcttc ctcgtcacc aaactgccc cctggggga 
      aaccgcccg gaggccgtc tccaagtac atcaaacgc 
      tcgctcgac ctgctccaa gtccaatat ctggatttg 
      ttcttgatc cacgtcccg tttgcgttc aaggacgtc 
      gagggggac ctgcatccc atgactcca gatgggcgc 
      atcgacatt gaccccagc accgaccac gtggcctta 
      tggaaggcg atggaagcg caactcgac gcaggactc 
      acaaaatcc atcggcctt tcaaacttc aaccagaag 
      cagattgag agagttttg caaaactgt cgcattccg 
      ccttccaat ctccaggtg gaacttcac gcgtatttg 
      cagcagaaa gagttggtc gagttttgc aaaacgaac 
      aaaatcgtt gtgacggct tactcacct ttggggtct 
      cccggattg gcgaaattt atggcccaa tttggacaa 
      aagattgat ttgccggat attttgaat aatccggta 
      gttaaagca attgcagcc aaacacaag aggagcgaa 
      gcccagatc gtgctgcgc catgcagtc cagaagggg 
      atcgtggtg attcccaaa agcaccaac ccgaagcgg 
      ctgcgggaa aacatcgac attttcggg ttcgagctg 
      gatcagggg gacatgagt cagttgaat ggtctggac 
      caggggatc aggattttg aattttgcc gcctttaag 
      gggattgag gagcaccca gagtacccg tttaaataa
    mRNA sequence
    • >TC015923 RA
      aaccacaac gctcaagcc ttagtttgg tgcgtactc 
      tcgcacatt tatcttcca tccaacgaa gtatttcca 
      ccggttttt gctaattta tgaattctt gtggtcttt 
      ttcgcgttt ctacgttta ttaaaagtt gggttttac 
      cggaaattt caacgcggc ggtcaagca gtaattttg 
      caaaaatga gtgtgctta cgactactt taaacaacg 
      gattgaaaa tgccaatga tagggtatg gaacctggc 
      aagcaaaag acgaagaac tggagcaag ccttcgaag 
      ccgccctgg aagccggat accgccata tcgacacag 
      ctcacgtct atgaaaatg aaaaagtta tcggcaaag 
      ttctaaacc ggtggctct cctcgggca aactaaccc 
      gcaaagaca tcttcctcg tcaccaaac tgccccctg 
      ggggaaacc gcccggagg ccgtctcca agtacatca 
      aacgctcgc tcgacctgc tccaagtcc aatatctgg 
      atttgttct tgatccacg tcccgtttg cgttcaagg 
      acgtcgagg gggacctgc atcccatga ctccagatg 
      ggcgcatcg acattgacc ccagcaccg accacgtgg 
      ccttatgga aggcgatgg aagcgcaac tcgacgcag 
      gactcacaa aatccatcg gcctttcaa acttcaacc 
      agaagcaga ttgagagag ttttgcaaa actgtcgca 
      ttccgcctt ccaatctcc aggtggaac ttcacgcgt 
      atttgcagc agaaagagt tggtcgagt tttgcaaaa 
      cgaacaaaa tcgttgtga cggcttact cacctttgg 
      ggtctcccg gattggcga aatttatgg cccaatttg 
      gacaaaaga ttgatttgc cggatattt tgaataatc 
      cggtagtta aagcaattg cagccaaac acaagagga 
      gcgaagccc agatcgtgc tgcgccatg cagtccaga 
      aggggatcg tggtgattc ccaaaagca ccaacccga 
      agcggctgc gggaaaaca tcgacattt tcgggttcg 
      agctggatc agggggaca tgagtcagt tgaatggtc 
      tggaccagg ggatcagga ttttgaatt ttgccgcct 
      ttaagggga ttgaggagc acccagagt acccgttta 
      aataaaaga ggcacgagt ttgtaaaaa atttattgt 
      attcttgta aggatatga cgtacagat agaacacga 
      aataattct tcgttgtag tttggtgtt tataagtaa 
      actatgtct taataaagt ttattgttt taatgcacc 
      taattgact cttcgcact agatgatat gcaatttag 
      taagaaatt ttaaaattt gaagcgttg ctgtctttt 
      aaacaggac cagcgataa aattgctag aaaagaaca 
      atgacactt tttacaaaa cgtaaaaag tggcttggg 
      agggcagtc gggttcggt gagaacatc gtaaatcaa 
      gaaagtttg aaactttga aatttggtt ggcaacact 
      taataaagc acagacaaa aatttgtaa atttttacg 
      cactctgat gtttctgat ttgctcatt tttaacgag 
      attttgcga aataattgc gttttctga gcaaaattg 
      ctctaattc tggctgtaa ggttttgtg tggggactt 
      tggtcttaa aacgtgcct aaaagaggt agaaaataa 
      cacaatctt tggtcaaac cttgcggtc ttttggata 
      atttctgac gaatttttg ctcaaaata atattaatt 
      tcgatcgat ttttgtgac ttttggaaa taatttgac 
      aaaattttg ctaataaat gtgtttctg attcaaaag 
      gaacgaaat aagcgaaaa aggctaaaa taaataacg 
      tccaatgct aatttatca attattatc agccacttc 
      gagaagaaa ccttttatt ttttatgct taaaaactc 
      gaaaaatgc cacaataat aattgcgtt tctaagttc 
      atgtgctaa acaaacaat aaaacatta ggttatttt 
      tgcactaat ttagtgttt tttcgactt tttttggcg 
      aaattttgt ctgaaaaca agtaaatta atcagaaag 
      caaccaaat caagcgaaa gatattaaa ataataaca 
      atttcaatc tgagatgct tagctgatt ctgtcaaaa 
      tttttgcag aatttatca aaatacttg cgttttttc 
      ctaagaaat ggaaaaaaa cgctaaatt aatgtcttt 
      tttgttgaa tttaacaat ttttgaacc tgtttacta 
      aataaaact caccacgaa ac