General information | |||
TC034777 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 4213296..4219875 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034777 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02529 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
- >TC034777 RA
MASGVEVNG MNGSPRKRA RTAPQAGHE SKVIVILGA QWGDEGKGK VVDMLAETV DIVCRCQGG NNAGHTVVI EGVEYDFHL LPSGIIHPK CHSVIGNGV VIHLPGLFE EVEKNEKKG LKDWESRLI ISDRAHLVF DFHQQVDGL QEQENTQKG QGLGTTKKG IGPTYSSKA TRNGIRIGE LLGDFSQFS KKFQGLVNV YQRMFPSLT VDVESELAR YKVYAERVR PLVYETVSY LDKAIKSGK KVLVEGANA AMLDIDFGT YPYVTSSNC SVGGVCTGL GLSPNRIGE VIGVVKAYT TRVGDGPFP TELRDKIGD CLQERGAEV GVTTKRKRR CGWLDVFLL QYTNIVNGY TALCLTKLD ILDTLPEIK LGVGYKLKG KKIDYFPSS AAELAEVEV EYITLPGWK TSTENVRKF NDLPENAKK YVNKVEQLV NVPVKWIGV GKGRESIIT LEE
- >TC034777 RA
atggcgagt ggcgtggaa gtcaacggc atgaatggc tcgccccga aagcgggcg cggaccgcc ccccaagcg ggccacgag tccaaagtc atcgtgatc ctgggggcg caatggggc gacgaaggc aaggggaag gtcgtcgat atgctggcc gaaactgtt gacatcgtt tgccggtgt cagggtgga aataatgca ggtcataca gtggtaatt gaaggggtg gaatacgac ttccatctc ttgcccagt gggattata catcccaaa tgccactcg gtaattggc aacggtgtc gtcatccac ttgcccggc cttttcgaa gaagtggag aagaacgag aaaaaaggc ctcaaagac tgggagagc cgactgatt atctcagac cgtgcccac ctagttttt gatttccac caacaagtc gatggccta caagaacag gaaaatacg caaaaaggc caaggtttg ggcaccacg aagaaggga atcggaccg acttactcg tccaaagcg accagaaat gggattcgg attggggaa ctcttgggc gatttttca caatttagc aaaaagttt caaggtttg gttaatgtg taccagagg atgtttccg tcgttgact gttgatgtc gagagtgaa ttggcaagg tataaggtt tatgccgag agagtgagg cctttggtg tacgaaacc gtttcctat cttgataaa gctataaaa tcagggaag aaggttcta gttgaaggt gctaatgcg gcgatgctg gacatcgat tttggtact tacccttat gttacttct tccaattgt agcgtgggt ggggtgtgt acaggatta ggtttatca cctaacaga attggcgaa gttattgga gttgtcaag gcctacacg acccgtgtt ggcgatggc ccttttccc actgaacta cgtgataaa atcggcgat tgtttgcaa gaaagagga gccgaagtc ggtgttaca acaaaacga aaacgtcgt tgcggttgg ttggacgtc tttctcctc caatacact aacatcgtt aacggctac actgctctg tgcctaaca aaactcgac attttagac actttgcct gaaattaag ttaggagtt gggtacaag ttgaagggg aaaaaaatc gactatttt ccgtccagt gcggccgag cttgccgaa gttgaggtt gagtatata acgcttccg ggctggaaa acctcgacc gaaaacgtg cgaaaattc aacgacttg ccggaaaac gcgaaaaag tacgtcaac aaggtggag cagttggtt aatgtgcca gtgaaatgg attggtgtt gggaaagga cgcgagtcg ataatcaca ctagaagag tga
- >TC034777 RA
ccgctcaac gacgcacgt cagttctcc ttcggtgtc tttgtggtg gtgcttgac cgtgcaaga aatggcgag tggcgtgga agtcaacgg catgaatgg ctcgccccg aaagcgggc gcggaccgc cccccaagc gggccacga gtccaaagt catcgtgat cctgggggc gcaatgggg cgacgaagg caaggggaa ggtcgtcga tatgctggc cgaaactgt tgacatcgt ttgccggtg tcagggtgg aaataatgc aggtcatac agtggtaat tgaaggggt ggaatacga cttccatct cttgcccag tgggattat acatcccaa atgccactc ggtaattgg caacggtgt cgtcatcca cttgcccgg ccttttcga agaagtgga gaagaacga gaaaaaagg cctcaaaga ctgggagag ccgactgat tatctcaga ccgtgccca cctagtttt tgatttcca ccaacaagt cgatggcct acaagaaca ggaaaatac gcaaaaagg ccaaggttt gggcaccac gaagaaggg aatcggacc gacttactc gtccaaagc gaccagaaa tgggattcg gattgggga actcttggg cgatttttc acaatttag caaaaagtt tcaaggttt ggttaatgt gtaccagag gatgtttcc gtcgttgac tgttgatgt cgagagtga attggcaag gtataaggt ttatgccga gagagtgag gcctttggt gtacgaaac cgtttccta tcttgataa agctataaa atcagggaa gaaggttct agttgaagg tgctaatgc ggcgatgct ggacatcga ttttggtac ttaccctta tgttacttc ttccaattg tagcgtggg tggggtgtg tacaggatt aggtttatc acctaacag aattggcga agttattgg agttgtcaa ggcctacac gacccgtgt tggcgatgg cccttttcc cactgaact acgtgataa aatcggcga ttgtttgca agaaagagg agccgaagt cggtgttac aacaaaacg aaaacgtcg ttgcggttg gttggacgt ctttctcct ccaatacac taacatcgt taacggcta cactgctct gtgcctaac aaaactcga cattttaga cactttgcc tgaaattaa gttaggagt tgggtacaa gttgaaggg gaaaaaaat cgactattt tccgtccag tgcggccga gcttgccga agttgaggt tgagtatat aacgcttcc gggctggaa aacctcgac cgaaaacgt gcgaaaatt caacgactt gccggaaaa cgcgaaaaa gtacgtcaa caaggtgga gcagttggt taatgtgcc agtgaaatg gattggtgt tgggaaagg acgcgagtc gataatcac actagaaga gtgattcaa gttttacat agtcataaa tgcacattc aatacttaa atttcaaag ataacattc atttatcaa agtaaaaca tgcaatagt attagatgt acaagtaca aatctagta tttaacttg agtatttgt cggttttaa atgaatttt tttgtttgt ttgcacgtg ttttatgca cataaacgt cgcaactcg gttaaatca tggcttcca gaaacaaac tgtgcaatt gactgaatc agtaagtaa ggttttggt gcctaagaa atttgtaaa tacttgttt aaaaatttc atcaagttg ttttggctc attttggtt tttttcaac tttgatttg cgtgtctta ctgtacttt ggattaaag ggacaatgg aaatgcagc aagtacaaa attatttta atttttcgt tccaatgta ggttttaca tcaaatatg tattttatt gaatttttt gctttttaa agtgttaac agttttatc caaacggtt tagactaac ttctgtttt taattgtgg ttgtgtaag tacttaatt ttgcacttg atgctttac cattaatct cggttttgg ctgttgtac aaataaaaa acataaaca aac
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
