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Gene information
General information
TC034777 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4213296..4219875
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034777
    iBeetle screen iB_02529

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 2
    vitellogenic egg chamber degenerated - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 70.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034777 RA
      MASGVEVNG MNGSPRKRA RTAPQAGHE SKVIVILGA 
      QWGDEGKGK VVDMLAETV DIVCRCQGG NNAGHTVVI 
      EGVEYDFHL LPSGIIHPK CHSVIGNGV VIHLPGLFE 
      EVEKNEKKG LKDWESRLI ISDRAHLVF DFHQQVDGL 
      QEQENTQKG QGLGTTKKG IGPTYSSKA TRNGIRIGE 
      LLGDFSQFS KKFQGLVNV YQRMFPSLT VDVESELAR 
      YKVYAERVR PLVYETVSY LDKAIKSGK KVLVEGANA 
      AMLDIDFGT YPYVTSSNC SVGGVCTGL GLSPNRIGE 
      VIGVVKAYT TRVGDGPFP TELRDKIGD CLQERGAEV 
      GVTTKRKRR CGWLDVFLL QYTNIVNGY TALCLTKLD 
      ILDTLPEIK LGVGYKLKG KKIDYFPSS AAELAEVEV 
      EYITLPGWK TSTENVRKF NDLPENAKK YVNKVEQLV 
      NVPVKWIGV GKGRESIIT LEE
    CDS sequence
    • >TC034777 RA
      atggcgagt ggcgtggaa gtcaacggc atgaatggc 
      tcgccccga aagcgggcg cggaccgcc ccccaagcg 
      ggccacgag tccaaagtc atcgtgatc ctgggggcg 
      caatggggc gacgaaggc aaggggaag gtcgtcgat 
      atgctggcc gaaactgtt gacatcgtt tgccggtgt 
      cagggtgga aataatgca ggtcataca gtggtaatt 
      gaaggggtg gaatacgac ttccatctc ttgcccagt 
      gggattata catcccaaa tgccactcg gtaattggc 
      aacggtgtc gtcatccac ttgcccggc cttttcgaa 
      gaagtggag aagaacgag aaaaaaggc ctcaaagac 
      tgggagagc cgactgatt atctcagac cgtgcccac 
      ctagttttt gatttccac caacaagtc gatggccta 
      caagaacag gaaaatacg caaaaaggc caaggtttg 
      ggcaccacg aagaaggga atcggaccg acttactcg 
      tccaaagcg accagaaat gggattcgg attggggaa 
      ctcttgggc gatttttca caatttagc aaaaagttt 
      caaggtttg gttaatgtg taccagagg atgtttccg 
      tcgttgact gttgatgtc gagagtgaa ttggcaagg 
      tataaggtt tatgccgag agagtgagg cctttggtg 
      tacgaaacc gtttcctat cttgataaa gctataaaa 
      tcagggaag aaggttcta gttgaaggt gctaatgcg 
      gcgatgctg gacatcgat tttggtact tacccttat 
      gttacttct tccaattgt agcgtgggt ggggtgtgt 
      acaggatta ggtttatca cctaacaga attggcgaa 
      gttattgga gttgtcaag gcctacacg acccgtgtt 
      ggcgatggc ccttttccc actgaacta cgtgataaa 
      atcggcgat tgtttgcaa gaaagagga gccgaagtc 
      ggtgttaca acaaaacga aaacgtcgt tgcggttgg 
      ttggacgtc tttctcctc caatacact aacatcgtt 
      aacggctac actgctctg tgcctaaca aaactcgac 
      attttagac actttgcct gaaattaag ttaggagtt 
      gggtacaag ttgaagggg aaaaaaatc gactatttt 
      ccgtccagt gcggccgag cttgccgaa gttgaggtt 
      gagtatata acgcttccg ggctggaaa acctcgacc 
      gaaaacgtg cgaaaattc aacgacttg ccggaaaac 
      gcgaaaaag tacgtcaac aaggtggag cagttggtt 
      aatgtgcca gtgaaatgg attggtgtt gggaaagga 
      cgcgagtcg ataatcaca ctagaagag tga
    mRNA sequence
    • >TC034777 RA
      ccgctcaac gacgcacgt cagttctcc ttcggtgtc 
      tttgtggtg gtgcttgac cgtgcaaga aatggcgag 
      tggcgtgga agtcaacgg catgaatgg ctcgccccg 
      aaagcgggc gcggaccgc cccccaagc gggccacga 
      gtccaaagt catcgtgat cctgggggc gcaatgggg 
      cgacgaagg caaggggaa ggtcgtcga tatgctggc 
      cgaaactgt tgacatcgt ttgccggtg tcagggtgg 
      aaataatgc aggtcatac agtggtaat tgaaggggt 
      ggaatacga cttccatct cttgcccag tgggattat 
      acatcccaa atgccactc ggtaattgg caacggtgt 
      cgtcatcca cttgcccgg ccttttcga agaagtgga 
      gaagaacga gaaaaaagg cctcaaaga ctgggagag 
      ccgactgat tatctcaga ccgtgccca cctagtttt 
      tgatttcca ccaacaagt cgatggcct acaagaaca 
      ggaaaatac gcaaaaagg ccaaggttt gggcaccac 
      gaagaaggg aatcggacc gacttactc gtccaaagc 
      gaccagaaa tgggattcg gattgggga actcttggg 
      cgatttttc acaatttag caaaaagtt tcaaggttt 
      ggttaatgt gtaccagag gatgtttcc gtcgttgac 
      tgttgatgt cgagagtga attggcaag gtataaggt 
      ttatgccga gagagtgag gcctttggt gtacgaaac 
      cgtttccta tcttgataa agctataaa atcagggaa 
      gaaggttct agttgaagg tgctaatgc ggcgatgct 
      ggacatcga ttttggtac ttaccctta tgttacttc 
      ttccaattg tagcgtggg tggggtgtg tacaggatt 
      aggtttatc acctaacag aattggcga agttattgg 
      agttgtcaa ggcctacac gacccgtgt tggcgatgg 
      cccttttcc cactgaact acgtgataa aatcggcga 
      ttgtttgca agaaagagg agccgaagt cggtgttac 
      aacaaaacg aaaacgtcg ttgcggttg gttggacgt 
      ctttctcct ccaatacac taacatcgt taacggcta 
      cactgctct gtgcctaac aaaactcga cattttaga 
      cactttgcc tgaaattaa gttaggagt tgggtacaa 
      gttgaaggg gaaaaaaat cgactattt tccgtccag 
      tgcggccga gcttgccga agttgaggt tgagtatat 
      aacgcttcc gggctggaa aacctcgac cgaaaacgt 
      gcgaaaatt caacgactt gccggaaaa cgcgaaaaa 
      gtacgtcaa caaggtgga gcagttggt taatgtgcc 
      agtgaaatg gattggtgt tgggaaagg acgcgagtc 
      gataatcac actagaaga gtgattcaa gttttacat 
      agtcataaa tgcacattc aatacttaa atttcaaag 
      ataacattc atttatcaa agtaaaaca tgcaatagt 
      attagatgt acaagtaca aatctagta tttaacttg 
      agtatttgt cggttttaa atgaatttt tttgtttgt 
      ttgcacgtg ttttatgca cataaacgt cgcaactcg 
      gttaaatca tggcttcca gaaacaaac tgtgcaatt 
      gactgaatc agtaagtaa ggttttggt gcctaagaa 
      atttgtaaa tacttgttt aaaaatttc atcaagttg 
      ttttggctc attttggtt tttttcaac tttgatttg 
      cgtgtctta ctgtacttt ggattaaag ggacaatgg 
      aaatgcagc aagtacaaa attatttta atttttcgt 
      tccaatgta ggttttaca tcaaatatg tattttatt 
      gaatttttt gctttttaa agtgttaac agttttatc 
      caaacggtt tagactaac ttctgtttt taattgtgg 
      ttgtgtaag tacttaatt ttgcacttg atgctttac 
      cattaatct cggttttgg ctgttgtac aaataaaaa 
      acataaaca aac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY