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Gene information
General information
TC015912 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 4129230..4133221
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015912
    iBeetle screen iB_02526

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015912 RA
      MDIADSGVQ EEAFYVCDV GDIVRKHKT WKAFLPRVQ 
      PHYAVKCND SLTVLEVLA ALGTGFDCA SKGEINKVL 
      SLGVSPDRI IFANPAKPS SHIRHAAAT NVSTMTFDN 
      ETELHKIKN FHPDSKLVI RIRCDATDS QCPLGMKFG 
      CDPVLEAPR LLQTARSLN LNVIGVSFH VGSGCREPS 
      VFKRAIAMS REVFDLGQA LGYNFTLLD IGGGFPGDR 
      GTSLDEIAQ VVNSALDDY FPDPSVEII AEPGRFYVS 
      SAYTLICNI HSIRDVISE DETTRHRMY YINDGVYGS 
      FNCILYDHQ RVVPLPLHE VPGSKYYSS SIWGPTCDG 
      LDQVVEEVL LPEMGLGDW LVFENMGAY TLPVASPFN 
      GFPVAKVHV VADESIWVL LKDIMPLTE EHFVMGDLP 
      ANVSIESGG NVAIELPIR MQVTGNDSI IEEHVLEFA 
      F
    CDS sequence
    • >TC015912 RA
      atggacatt gccgattct ggagtacag gaagaagct 
      ttttatgtg tgcgacgtt ggagatatt gtcaggaag 
      cacaagact tggaaggcg tttttgccg agagtacaa 
      ccacattat gctgttaaa tgcaacgac agcctgacc 
      gtccttgaa gtccttgcc gctttgggc accggcttc 
      gactgcgct tccaaaggc gagatcaac aaagtcctc 
      tccctcggc gtcagccct gaccgcatc atcttcgca 
      aatcccgcc aagccctca agtcacatc cgacatgct 
      gcagccacc aacgtttca accatgacc ttcgacaat 
      gaaaccgaa ctccacaaa atcaaaaat ttccacccc 
      gactcgaaa ctcgtcatt cgcattcgt tgcgacgcc 
      accgactcc caatgcccg ctcggcatg aaattcggc 
      tgcgacccc gtcctggaa gccccccga ctgctgcaa 
      acagcccgc agcctcaat ctcaacgtg atcggcgtg 
      agctttcac gtgggctcg ggctgtcgc gaaccttcg 
      gtctttaaa cgagccatt gccatgtct cgtgaagtg 
      ttcgacctg gggcaagcc ctcggctac aacttcacg 
      ctcctggac attggcggg gggttccca ggcgaccgc 
      ggaacttcg cttgacgaa atcgcacaa gttgtgaat 
      tcggccctt gatgattat ttcccggac ccgtctgtg 
      gaaataatc gcagaaccg gggcgtttt tacgtcagt 
      tctgcctac acgctcatt tgcaacatt cattcgatt 
      cgggacgtt atcagcgaa gatgagaca acgcgtcat 
      aggatgtac tacatcaat gatggggtt tatggaagt 
      ttcaattgt attttgtac gatcatcaa cgggtggtt 
      cccctgccg ttgcatgaa gtccccggg tcgaagtac 
      tactcgagt agcatctgg gggcccacg tgtgacggg 
      ttggaccag gttgttgag gaggttttg cttccggag 
      atggggctt ggcgattgg ctcgttttt gaaaacatg 
      ggggcttat actcttcca gttgcgagt ccttttaac 
      ggttttccc gtggcgaaa gtacacgtg gtggccgat 
      gaaagtatc tgggtgctg ttgaaggat ataatgccg 
      ctgacggag gagcatttc gtcatgggg gatttaccg 
      gctaatgtc agtattgaa agtggagga aacgtcgct 
      attgaacta ccgattagg atgcaagtc accggaaat 
      gactccatt attgaggag cacgttctc gagtttgca 
      ttctag
    mRNA sequence
    • >TC015912 RA
      cacttataa tagtgtttt gtacgaaaa tgtacccaa 
      aaaataatt ttcttaggg ttgcattgt ggacgaagc 
      ttccccggc tactagcag tgcatttct cccatacaa 
      ttggagaat attcttgaa cggagaacc tccttggaa 
      ctgctgatt cccgcagcg aaaaagtaa cacccgctt 
      tttaagaca tcctgagca actctgagc acaggttgc 
      ctccttgct tattacaac acaaactct taaatacat 
      ttaataatt atatcttta taccatttt aattcaaac 
      aaacaagat gaaattcac aaatacaga tgaacgcgt 
      tcacgtttt ggatgcaca atctaatgt ttggtccgt 
      cattatgga cattgccga ttctggagt acaggaaga 
      agcttttta tgtgtgcga cgttggaga tattgtcag 
      gaagcacaa gacttggaa ggcgttttt gccgagagt 
      acaaccaca ttatgctgt taaatgcaa cgacagcct 
      gaccgtcct tgaagtcct tgccgcttt gggcaccgg 
      cttcgactg cgcttccaa aggcgagat caacaaagt 
      cctctccct cggcgtcag ccctgaccg catcatctt 
      cgcaaatcc cgccaagcc ctcaagtca catccgaca 
      tgctgcagc caccaacgt ttcaaccat gaccttcga 
      caatgaaac cgaactcca caaaatcaa aaatttcca 
      ccccgactc gaaactcgt cattcgcat tcgttgcga 
      cgccaccga ctcccaatg cccgctcgg catgaaatt 
      cggctgcga ccccgtcct ggaagcccc ccgactgct 
      gcaaacagc ccgcagcct caatctcaa cgtgatcgg 
      cgtgagctt tcacgtggg ctcgggctg tcgcgaacc 
      ttcggtctt taaacgagc cattgccat gtctcgtga 
      agtgttcga cctggggca agccctcgg ctacaactt 
      cacgctcct ggacattgg cggggggtt cccaggcga 
      ccgcggaac ttcgcttga cgaaatcgc acaagttgt 
      gaattcggc ccttgatga ttatttccc ggacccgtc 
      tgtggaaat aatcgcaga accggggcg tttttacgt 
      cagttctgc ctacacgct catttgcaa cattcattc 
      gattcggga cgttatcag cgaagatga gacaacgcg 
      tcataggat gtactacat caatgatgg ggtttatgg 
      aagtttcaa ttgtatttt gtacgatca tcaacgggt 
      ggttcccct gccgttgca tgaagtccc cgggtcgaa 
      gtactactc gagtagcat ctgggggcc cacgtgtga 
      cgggttgga ccaggttgt tgaggaggt tttgcttcc 
      ggagatggg gcttggcga ttggctcgt ttttgaaaa 
      catgggggc ttatactct tccagttgc gagtccttt 
      taacggttt tcccgtggc gaaagtaca cgtggtggc 
      cgatgaaag tatctgggt gctgttgaa ggatataat 
      gccgctgac ggaggagca tttcgtcat gggggattt 
      accggctaa tgtcagtat tgaaagtgg aggaaacgt 
      cgctattga actaccgat taggatgca agtcaccgg 
      aaatgactc cattattga ggagcacgt tctcgagtt 
      tgcattcta gcataaata tgcttcgat aggggtaaa 
      aatattgga aaaattgaa aaaaaatat aaaaaaccg 
      gtttctttt tatactagt atagagata taattgtaa 
      aatgttttt agtagtgcg aatatttct attcgataa 
      tattagaac aggtattat tggctagga atattcata 
      gtataagca agggggcaa ctaatgctt atttacttt 
      ggattcaac ttaatgaca gtctagtac ttttctggc 
      ttgaaaatt cactagggt gttttattg ttctattac 
      tgtgcttgt agatgtaga aattataaa ataaaactt 
      gcaatcaag c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY