Status
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Gene information
General information
TC033609
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015895,TC015896 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3991103..4000050
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC033609
    iBeetle screen iB_02525

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033609 RA
      MKVGVFLAL CASVRDKPT KISRTIVRP IHDLKIALL 
SVVKPRLEP REISPVDQI LRRRSSVLE PTMVYESDF 
YTTRRPYRP SYSTYSVSK REIPWEKVP FVPRPSLVP 
DPVTAFGKR KTVEEVFRP DRKNILSPN ERAKIKPKI 
LLEPPKPYL SARDINREK ILNSIKVRE SQLNDSMDV 
LLPRLHKVA ENPNLPGCW DCVCCPQSP RHYLVRDSP 
DRSRRAAEE VYSYSYSAE SSRSGDPYS RPSTRSYTT 
SESVNRSSN SGPGSYSYS TERSSRSYG DGPGGYHSS 
YSSSTSGRL PGGTTYRHY SYRSAPSRQ VRILPGLGK 
VHVVHTYDR IVPYVGHKR LTVVTLPPK IYSTRSSVL 
QREYDWIEN KVRPWVAYS PTNRYLNSD SAVRYVYVR 
NPITGLKYH SDYYPSRYY NFYRIIRPL SYYDDYWPF 
YRPLSAYYS YWPISRSVS YLDDIPLYR SLRLQSIFD 
DETRLIRAQ TASLLKQVH QPVPRIRTW PITPLNRFG 
DFPSLPMKY SNDTYIHRL LTYSPNHKI QYATYYTEP 
VKKYIGDKG YSRRRPLTM FEDALRNDI QLLSYYITK 
FRDEKQQPK EIKAPLSPA RPSRIFSVH KPMEDPVAQ 
EIKDLREAR AQRLAKIYA SDDKTTDEV EKSLKEKKR 
KEEARLAEE RAIAEEQAK KEAEAARKA EIARQEQLA 
AQLAAERAA ELERQAEQA RQEELAKQA EIERMKREE 
EERARLEEQ RRLEEEAKA EEERQQLLR LEEIARQAE 
EEKERELAR QAEELAELA RQEAERAEQ ERLEREQKE 
KEQKEAELA ELARQETEL AELERQEKE LAELAAKAQ 
EELAANEEE EEKTEEQAE PVVDEPEAE AQEEEPAEV 
EPEDEPEAE AEADE
    CDS sequence
    • >TC033609 RA
      atgaaagtg ggcgtattt ttggccctt tgtgcatcc 
gttcgtgac aaacccacg aaaataagc cgcacaatc 
gtgcgtccg attcacgat ttaaaaata gccctcctg 
agcgttgtt aagcccagg cttgagccg cgtgaaatc 
agtccagtt gatcagatc ttgagacgc cgatcgtca 
gtcctcgaa cccaccatg gtatacgaa tccgacttc 
tacacgacc agaaggccc taccgccct tcgtactcg 
acgtacagc gtatcgaaa cgcgagatt ccgtgggag 
aaagtgcct tttgtccca agaccaagt ctggtgccg 
gaccccgtc acggccttc ggcaaaagg aaaacggtg 
gaggaagtc tttcgaccc gaccgaaaa aatattctt 
tccccgaac gaaagagcc aaaatcaaa ccgaaaatc 
ctcctcgaa ccgcccaaa ccctaccta tcggcgcgc 
gacatcaac agagaaaaa attctcaat tcgattaaa 
gtcagagag agtcagtta aacgactcc atggacgtg 
cttttgccc agactgcac aaagtcgcc gaaaacccc 
aatctcccc ggatgttgg gattgtgtt tgttgtcct 
cagagtccg agacattac ctggtgcgc gactcgcca 
gaccgcagc cgtcgcgct gccgaagag gtctattcg 
tactcgtat tctgcggaa tcgagccgc tctggtgac 
ccttactca cgtccaagc acccgttct tataccacg 
agcgagagt gtaaatcgt tcctcgaat tctgggcca 
ggttcttac tcttacagc actgaaagg tcctccagg 
agctacggg gacgggcct gggggctac cactccagc 
tacagctcg agcacctcc ggacgactc ccagggggc 
acaacctac cgccactat tcttaccgc tccgcccca 
tcaagacaa gtaaggatt cttcccgga ctcggtaaa 
gttcatgtg gtccacacc tacgaccgc atcgtacca 
tatgtgggt cacaaaaga ttgaccgtg gtaacgcta 
ccaccaaaa atttactca acacggtcg tccgtccta 
caaagggag tatgactgg atcgaaaat aaagtccgg 
ccctgggtt gcctattcc cccacaaac cgttacctc 
aactctgac tcagctgtg agatacgtc tatgttcgc 
aatccaata actggctta aagtatcat tcagactat 
tatccgtct cgatattac aacttttat cgaataatt 
cgcccgctt tcgtattac gacgattac tggccgttt 
tataggccg ttaagtgcg tattacagt tactggcct 
atttctcgt tcagttagc tatctcgat gatattccg 
ctttatcgt tctcttaga ttacagagt atatttgac 
gacgaaacg aggctgatt cgagctcag acggcgtct 
ctcctcaaa caggtccat cagccggta ccgagaatc 
agaacctgg cccattact ccgttaaac agattcggc 
gatttcccg tcgttgccg atgaaatac agcaacgac 
acttacatt cacagactc ttgacttat tccccaaat 
cacaaaata caatacgcc acatattac acggaaccc 
gttaaaaaa tacatcggg gacaaaggc tacagccgc 
cgtcgtcct ctgaccatg tttgaggac gctctcagg 
aatgatatc cagttgttg agttattac attactaag 
tttcgagat gagaaacaa caaccgaaa gaaattaaa 
gcaccttta tcgccagct cgtccttca cgtattttc 
tcagttcac aagccaatg gaagatccg gtcgctcaa 
gaaattaaa gatttgcgt gaagcacgg gcacagcgt 
ctcgctaaa atctacgca tctgatgat aaaacaaca 
gatgaagta gagaaaagt ctgaaggaa aagaaaagg 
aaagaagaa gcgaggtta gctgaagaa agagcaatt 
gctgaagag caagcgaag aaggaagcc gaagcggcc 
agaaaagca gaaatagct cgtcaagaa cagcttgca 
gctcaactc gccgcagaa cgtgcagca gaattagag 
aggcaggcg gaacaagca cgtcaggaa gaactggcc 
aaacaagct gagattgag cgaatgaag agagaggag 
gaggaaagg gcgcgcttg gaggaacag cgccgactt 
gaggaagaa gccaaagct gaagaagag cgccagcaa 
ctgctccgt ttggaggaa atcgctaga caagccgag 
gaggaaaaa gagcgagag ttggcgaga caagccgaa 
gaactggcg gagttagcg agacaagag gccgaacga 
gcggagcag gaacgtctc gaaagggaa caaaaggag 
aaggaacag aaggaagca gaacttgca gaactggcg 
agacaggaa accgaactg gccgaactg gaaagacag 
gaaaaggag ctggcggaa ctggcggcg aaagcgcaa 
gaagaactg gccgccaat gaagaagag gaagaaaag 
actgaagaa caagctgaa ccggtcgtt gatgaacct 
gaagctgaa gcacaagaa gaagaaccg gctgaagtc 
gagccggaa gacgagcct gaagccgaa gcagaagcc 
gatgaataa
    mRNA sequence
    • >TC033609 RA
      agaaactaa tttgttaag cgctaagtt caaaatagt 
tcatttaag gaaaaataa tataaggat taatatcca 
aatgaaagt gggcgtatt tttggccct ttgtgcatc 
cgttcgtga caaacccac gaaaataag ccgcacaat 
cgtgcgtcc gattcacga tttaaaaat agccctcct 
gagcgttgt taagcccag gcttgagcc gcgtgaaat 
cagtccagt tgatcagat cttgagacg ccgatcgtc 
agtcctcga acccaccat ggtatacga atccgactt 
ctacacgac cagaaggcc ctaccgccc ttcgtactc 
gacgtacag cgtatcgaa acgcgagat tccgtggga 
gaaagtgcc ttttgtccc aagaccaag tctggtgcc 
ggaccccgt cacggcctt cggcaaaag gaaaacggt 
ggaggaagt ctttcgacc cgaccgaaa aaatattct 
ttccccgaa cgaaagagc caaaatcaa accgaaaat 
cctcctcga accgcccaa accctacct atcggcgcg 
cgacatcaa cagagaaaa aattctcaa ttcgattaa 
agtcagaga gagtcagtt aaacgactc catggacgt 
gcttttgcc cagactgca caaagtcgc cgaaaaccc 
caatctccc cggatgttg ggattgtgt ttgttgtcc 
tcagagtcc gagacatta cctggtgcg cgactcgcc 
agaccgcag ccgtcgcgc tgccgaaga ggtctattc 
gtactcgta ttctgcgga atcgagccg ctctggtga 
cccttactc acgtccaag cacccgttc ttataccac 
gagcgagag tgtaaatcg ttcctcgaa ttctgggcc 
aggttctta ctcttacag cactgaaag gtcctccag 
gagctacgg ggacgggcc tgggggcta ccactccag 
ctacagctc gagcacctc cggacgact cccaggggg 
cacaaccta ccgccacta ttcttaccg ctccgcccc 
atcaagaca agtaaggat tcttcccgg actcggtaa 
agttcatgt ggtccacac ctacgaccg catcgtacc 
atatgtggg tcacaaaag attgaccgt ggtaacgct 
accaccaaa aatttactc aacacggtc gtccgtcct 
acaaaggga gtatgactg gatcgaaaa taaagtccg 
gccctgggt tgcctattc ccccacaaa ccgttacct 
caactctga ctcagctgt gagatacgt ctatgttcg 
caatccaat aactggctt aaagtatca ttcagacta 
ttatccgtc tcgatatta caactttta tcgaataat 
tcgcccgct ttcgtatta cgacgatta ctggccgtt 
ttataggcc gttaagtgc gtattacag ttactggcc 
tatttctcg ttcagttag ctatctcga tgatattcc 
gctttatcg ttctcttag attacagag tatatttga 
cgacgaaac gaggctgat tcgagctca gacggcgtc 
tctcctcaa acaggtcca tcagccggt accgagaat 
cagaacctg gcccattac tccgttaaa cagattcgg 
cgatttccc gtcgttgcc gatgaaata cagcaacga 
cacttacat tcacagact cttgactta ttccccaaa 
tcacaaaat acaatacgc cacatatta cacggaacc 
cgttaaaaa atacatcgg ggacaaagg ctacagccg 
ccgtcgtcc tctgaccat gtttgagga cgctctcag 
gaatgatat ccagttgtt gagttatta cattactaa 
gtttcgaga tgagaaaca acaaccgaa agaaattaa 
agcaccttt atcgccagc tcgtccttc acgtatttt 
ctcagttca caagccaat ggaagatcc ggtcgctca 
agaaattaa agatttgcg tgaagcacg ggcacagcg 
tctcgctaa aatctacgc atctgatga taaaacaac 
agatgaagt agagaaaag tctgaagga aaagaaaag 
gaaagaaga agcgaggtt agctgaaga aagagcaat 
tgctgaaga gcaagcgaa gaaggaagc cgaagcggc 
cagaaaagc agaaatagc tcgtcaaga acagcttgc 
agctcaact cgccgcaga acgtgcagc agaattaga 
gaggcaggc ggaacaagc acgtcagga agaactggc 
caaacaagc tgagattga gcgaatgaa gagagagga 
ggaggaaag ggcgcgctt ggaggaaca gcgccgact 
tgaggaaga agccaaagc tgaagaaga gcgccagca 
actgctccg tttggagga aatcgctag acaagccga 
ggaggaaaa agagcgaga gttggcgag acaagccga 
agaactggc ggagttagc gagacaaga ggccgaacg 
agcggagca ggaacgtct cgaaaggga acaaaagga 
gaaggaaca gaaggaagc agaacttgc agaactggc 
gagacagga aaccgaact ggccgaact ggaaagaca 
ggaaaagga gctggcgga actggcggc gaaagcgca 
agaagaact ggccgccaa tgaagaaga ggaagaaaa 
gactgaaga acaagctga accggtcgt tgatgaacc 
tgaagctga agcacaaga agaagaacc ggctgaagt 
cgagccgga agacgagcc tgaagccga agcagaagc 
cgatgaata aacccagtt taaacaatt tctcatttc 
taacacaca ttccaaact gtcgtgact caagcattt 
tgttgccaa tttcacgat agtctggaa tgaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY