Status
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Gene information
General information
TC033609
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC015895,TC015896
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid47: 1909203..1918150
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033609
    iBeetle screen iB_02525
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033609 RA
      MKVGVFLAL CASVRDKPT KISRTIVRP IHDLKIALL 
      SVVKPRLEP REISPVDQI LRRRSSVLE PTMVYESDF 
      YTTRRPYRP SYSTYSVSK REIPWEKVP FVPRPSLVP 
      DPVTAFGKR KTVEEVFRP DRKNILSPN ERAKIKPKI 
      LLEPPKPYL SARDINREK ILNSIKVRE SQLNDSMDV 
      LLPRLHKVA ENPNLPGCW DCVCCPQSP RHYLVRDSP 
      DRSRRAAEE VYSYSYSAE SSRSGDPYS RPSTRSYTT 
      SESVNRSSN SGPGSYSYS TERSSRSYG DGPGGYHSS 
      YSSSTSGRL PGGTTYRHY SYRSAPSRQ VRILPGLGK 
      VHVVHTYDR IVPYVGHKR LTVVTLPPK IYSTRSSVL 
      QREYDWIEN KVRPWVAYS PTNRYLNSD SAVRYVYVR 
      NPITGLKYH SDYYPSRYY NFYRIIRPL SYYDDYWPF 
      YRPLSAYYS YWPISRSVS YLDDIPLYR SLRLQSIFD 
      DETRLIRAQ TASLLKQVH QPVPRIRTW PITPLNRFG 
      DFPSLPMKY SNDTYIHRL LTYSPNHKI QYATYYTEP 
      VKKYIGDKG YSRRRPLTM FEDALRNDI QLLSYYITK 
      FRDEKQQPK EIKAPLSPA RPSRIFSVH KPMEDPVAQ 
      EIKDLREAR AQRLAKIYA SDDKTTDEV EKSLKEKKR 
      KEEARLAEE RAIAEEQAK KEAEAARKA EIARQEQLA 
      AQLAAERAA ELERQAEQA RQEELAKQA EIERMKREE 
      EERARLEEQ RRLEEEAKA EEERQQLLR LEEIARQAE 
      EEKERELAR QAEELAELA RQEAERAEQ ERLEREQKE 
      KEQKEAELA ELARQETEL AELERQEKE LAELAAKAQ 
      EELAANEEE EEKTEEQAE PVVDEPEAE AQEEEPAEV 
      EPEDEPEAE AEADE
    CDS sequence
    • >TC033609 RA
      atgaaagtg ggcgtattt ttggccctt tgtgcatcc 
      gttcgtgac aaacccacg aaaataagc cgcacaatc 
      gtgcgtccg attcacgat ttaaaaata gccctcctg 
      agcgttgtt aagcccagg cttgagccg cgtgaaatc 
      agtccagtt gatcagatc ttgagacgc cgatcgtca 
      gtcctcgaa cccaccatg gtatacgaa tccgacttc 
      tacacgacc agaaggccc taccgccct tcgtactcg 
      acgtacagc gtatcgaaa cgcgagatt ccgtgggag 
      aaagtgcct tttgtccca agaccaagt ctggtgccg 
      gaccccgtc acggccttc ggcaaaagg aaaacggtg 
      gaggaagtc tttcgaccc gaccgaaaa aatattctt 
      tccccgaac gaaagagcc aaaatcaaa ccgaaaatc 
      ctcctcgaa ccgcccaaa ccctaccta tcggcgcgc 
      gacatcaac agagaaaaa attctcaat tcgattaaa 
      gtcagagag agtcagtta aacgactcc atggacgtg 
      cttttgccc agactgcac aaagtcgcc gaaaacccc 
      aatctcccc ggatgttgg gattgtgtt tgttgtcct 
      cagagtccg agacattac ctggtgcgc gactcgcca 
      gaccgcagc cgtcgcgct gccgaagag gtctattcg 
      tactcgtat tctgcggaa tcgagccgc tctggtgac 
      ccttactca cgtccaagc acccgttct tataccacg 
      agcgagagt gtaaatcgt tcctcgaat tctgggcca 
      ggttcttac tcttacagc actgaaagg tcctccagg 
      agctacggg gacgggcct gggggctac cactccagc 
      tacagctcg agcacctcc ggacgactc ccagggggc 
      acaacctac cgccactat tcttaccgc tccgcccca 
      tcaagacaa gtaaggatt cttcccgga ctcggtaaa 
      gttcatgtg gtccacacc tacgaccgc atcgtacca 
      tatgtgggt cacaaaaga ttgaccgtg gtaacgcta 
      ccaccaaaa atttactca acacggtcg tccgtccta 
      caaagggag tatgactgg atcgaaaat aaagtccgg 
      ccctgggtt gcctattcc cccacaaac cgttacctc 
      aactctgac tcagctgtg agatacgtc tatgttcgc 
      aatccaata actggctta aagtatcat tcagactat 
      tatccgtct cgatattac aacttttat cgaataatt 
      cgcccgctt tcgtattac gacgattac tggccgttt 
      tataggccg ttaagtgcg tattacagt tactggcct 
      atttctcgt tcagttagc tatctcgat gatattccg 
      ctttatcgt tctcttaga ttacagagt atatttgac 
      gacgaaacg aggctgatt cgagctcag acggcgtct 
      ctcctcaaa caggtccat cagccggta ccgagaatc 
      agaacctgg cccattact ccgttaaac agattcggc 
      gatttcccg tcgttgccg atgaaatac agcaacgac 
      acttacatt cacagactc ttgacttat tccccaaat 
      cacaaaata caatacgcc acatattac acggaaccc 
      gttaaaaaa tacatcggg gacaaaggc tacagccgc 
      cgtcgtcct ctgaccatg tttgaggac gctctcagg 
      aatgatatc cagttgttg agttattac attactaag 
      tttcgagat gagaaacaa caaccgaaa gaaattaaa 
      gcaccttta tcgccagct cgtccttca cgtattttc 
      tcagttcac aagccaatg gaagatccg gtcgctcaa 
      gaaattaaa gatttgcgt gaagcacgg gcacagcgt 
      ctcgctaaa atctacgca tctgatgat aaaacaaca 
      gatgaagta gagaaaagt ctgaaggaa aagaaaagg 
      aaagaagaa gcgaggtta gctgaagaa agagcaatt 
      gctgaagag caagcgaag aaggaagcc gaagcggcc 
      agaaaagca gaaatagct cgtcaagaa cagcttgca 
      gctcaactc gccgcagaa cgtgcagca gaattagag 
      aggcaggcg gaacaagca cgtcaggaa gaactggcc 
      aaacaagct gagattgag cgaatgaag agagaggag 
      gaggaaagg gcgcgcttg gaggaacag cgccgactt 
      gaggaagaa gccaaagct gaagaagag cgccagcaa 
      ctgctccgt ttggaggaa atcgctaga caagccgag 
      gaggaaaaa gagcgagag ttggcgaga caagccgaa 
      gaactggcg gagttagcg agacaagag gccgaacga 
      gcggagcag gaacgtctc gaaagggaa caaaaggag 
      aaggaacag aaggaagca gaacttgca gaactggcg 
      agacaggaa accgaactg gccgaactg gaaagacag 
      gaaaaggag ctggcggaa ctggcggcg aaagcgcaa 
      gaagaactg gccgccaat gaagaagag gaagaaaag 
      actgaagaa caagctgaa ccggtcgtt gatgaacct 
      gaagctgaa gcacaagaa gaagaaccg gctgaagtc 
      gagccggaa gacgagcct gaagccgaa gcagaagcc 
      gatgaataa
    mRNA sequence
    • >TC033609 RA
      agaaactaa tttgttaag cgctaagtt caaaatagt 
      tcatttaag gaaaaataa tataaggat taatatcca 
      aatgaaagt gggcgtatt tttggccct ttgtgcatc 
      cgttcgtga caaacccac gaaaataag ccgcacaat 
      cgtgcgtcc gattcacga tttaaaaat agccctcct 
      gagcgttgt taagcccag gcttgagcc gcgtgaaat 
      cagtccagt tgatcagat cttgagacg ccgatcgtc 
      agtcctcga acccaccat ggtatacga atccgactt 
      ctacacgac cagaaggcc ctaccgccc ttcgtactc 
      gacgtacag cgtatcgaa acgcgagat tccgtggga 
      gaaagtgcc ttttgtccc aagaccaag tctggtgcc 
      ggaccccgt cacggcctt cggcaaaag gaaaacggt 
      ggaggaagt ctttcgacc cgaccgaaa aaatattct 
      ttccccgaa cgaaagagc caaaatcaa accgaaaat 
      cctcctcga accgcccaa accctacct atcggcgcg 
      cgacatcaa cagagaaaa aattctcaa ttcgattaa 
      agtcagaga gagtcagtt aaacgactc catggacgt 
      gcttttgcc cagactgca caaagtcgc cgaaaaccc 
      caatctccc cggatgttg ggattgtgt ttgttgtcc 
      tcagagtcc gagacatta cctggtgcg cgactcgcc 
      agaccgcag ccgtcgcgc tgccgaaga ggtctattc 
      gtactcgta ttctgcgga atcgagccg ctctggtga 
      cccttactc acgtccaag cacccgttc ttataccac 
      gagcgagag tgtaaatcg ttcctcgaa ttctgggcc 
      aggttctta ctcttacag cactgaaag gtcctccag 
      gagctacgg ggacgggcc tgggggcta ccactccag 
      ctacagctc gagcacctc cggacgact cccaggggg 
      cacaaccta ccgccacta ttcttaccg ctccgcccc 
      atcaagaca agtaaggat tcttcccgg actcggtaa 
      agttcatgt ggtccacac ctacgaccg catcgtacc 
      atatgtggg tcacaaaag attgaccgt ggtaacgct 
      accaccaaa aatttactc aacacggtc gtccgtcct 
      acaaaggga gtatgactg gatcgaaaa taaagtccg 
      gccctgggt tgcctattc ccccacaaa ccgttacct 
      caactctga ctcagctgt gagatacgt ctatgttcg 
      caatccaat aactggctt aaagtatca ttcagacta 
      ttatccgtc tcgatatta caactttta tcgaataat 
      tcgcccgct ttcgtatta cgacgatta ctggccgtt 
      ttataggcc gttaagtgc gtattacag ttactggcc 
      tatttctcg ttcagttag ctatctcga tgatattcc 
      gctttatcg ttctcttag attacagag tatatttga 
      cgacgaaac gaggctgat tcgagctca gacggcgtc 
      tctcctcaa acaggtcca tcagccggt accgagaat 
      cagaacctg gcccattac tccgttaaa cagattcgg 
      cgatttccc gtcgttgcc gatgaaata cagcaacga 
      cacttacat tcacagact cttgactta ttccccaaa 
      tcacaaaat acaatacgc cacatatta cacggaacc 
      cgttaaaaa atacatcgg ggacaaagg ctacagccg 
      ccgtcgtcc tctgaccat gtttgagga cgctctcag 
      gaatgatat ccagttgtt gagttatta cattactaa 
      gtttcgaga tgagaaaca acaaccgaa agaaattaa 
      agcaccttt atcgccagc tcgtccttc acgtatttt 
      ctcagttca caagccaat ggaagatcc ggtcgctca 
      agaaattaa agatttgcg tgaagcacg ggcacagcg 
      tctcgctaa aatctacgc atctgatga taaaacaac 
      agatgaagt agagaaaag tctgaagga aaagaaaag 
      gaaagaaga agcgaggtt agctgaaga aagagcaat 
      tgctgaaga gcaagcgaa gaaggaagc cgaagcggc 
      cagaaaagc agaaatagc tcgtcaaga acagcttgc 
      agctcaact cgccgcaga acgtgcagc agaattaga 
      gaggcaggc ggaacaagc acgtcagga agaactggc 
      caaacaagc tgagattga gcgaatgaa gagagagga 
      ggaggaaag ggcgcgctt ggaggaaca gcgccgact 
      tgaggaaga agccaaagc tgaagaaga gcgccagca 
      actgctccg tttggagga aatcgctag acaagccga 
      ggaggaaaa agagcgaga gttggcgag acaagccga 
      agaactggc ggagttagc gagacaaga ggccgaacg 
      agcggagca ggaacgtct cgaaaggga acaaaagga 
      gaaggaaca gaaggaagc agaacttgc agaactggc 
      gagacagga aaccgaact ggccgaact ggaaagaca 
      ggaaaagga gctggcgga actggcggc gaaagcgca 
      agaagaact ggccgccaa tgaagaaga ggaagaaaa 
      gactgaaga acaagctga accggtcgt tgatgaacc 
      tgaagctga agcacaaga agaagaacc ggctgaagt 
      cgagccgga agacgagcc tgaagccga agcagaagc 
      cgatgaata aacccagtt taaacaatt tctcatttc 
      taacacaca ttccaaact gtcgtgact caagcattt 
      tgttgccaa tttcacgat agtctggaa tgaaaa