| General information | |||
| TC033609
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3991103..4000050 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC033609 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_02525 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 33.33% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 44.44% (includes death as larva, prepupa, pupa, adult)
- >TC033609 RA
MKVGVFLAL CASVRDKPT KISRTIVRP IHDLKIALL SVVKPRLEP REISPVDQI LRRRSSVLE PTMVYESDF YTTRRPYRP SYSTYSVSK REIPWEKVP FVPRPSLVP DPVTAFGKR KTVEEVFRP DRKNILSPN ERAKIKPKI LLEPPKPYL SARDINREK ILNSIKVRE SQLNDSMDV LLPRLHKVA ENPNLPGCW DCVCCPQSP RHYLVRDSP DRSRRAAEE VYSYSYSAE SSRSGDPYS RPSTRSYTT SESVNRSSN SGPGSYSYS TERSSRSYG DGPGGYHSS YSSSTSGRL PGGTTYRHY SYRSAPSRQ VRILPGLGK VHVVHTYDR IVPYVGHKR LTVVTLPPK IYSTRSSVL QREYDWIEN KVRPWVAYS PTNRYLNSD SAVRYVYVR NPITGLKYH SDYYPSRYY NFYRIIRPL SYYDDYWPF YRPLSAYYS YWPISRSVS YLDDIPLYR SLRLQSIFD DETRLIRAQ TASLLKQVH QPVPRIRTW PITPLNRFG DFPSLPMKY SNDTYIHRL LTYSPNHKI QYATYYTEP VKKYIGDKG YSRRRPLTM FEDALRNDI QLLSYYITK FRDEKQQPK EIKAPLSPA RPSRIFSVH KPMEDPVAQ EIKDLREAR AQRLAKIYA SDDKTTDEV EKSLKEKKR KEEARLAEE RAIAEEQAK KEAEAARKA EIARQEQLA AQLAAERAA ELERQAEQA RQEELAKQA EIERMKREE EERARLEEQ RRLEEEAKA EEERQQLLR LEEIARQAE EEKERELAR QAEELAELA RQEAERAEQ ERLEREQKE KEQKEAELA ELARQETEL AELERQEKE LAELAAKAQ EELAANEEE EEKTEEQAE PVVDEPEAE AQEEEPAEV EPEDEPEAE AEADE
- >TC033609 RA
atgaaagtg ggcgtattt ttggccctt tgtgcatcc gttcgtgac aaacccacg aaaataagc cgcacaatc gtgcgtccg attcacgat ttaaaaata gccctcctg agcgttgtt aagcccagg cttgagccg cgtgaaatc agtccagtt gatcagatc ttgagacgc cgatcgtca gtcctcgaa cccaccatg gtatacgaa tccgacttc tacacgacc agaaggccc taccgccct tcgtactcg acgtacagc gtatcgaaa cgcgagatt ccgtgggag aaagtgcct tttgtccca agaccaagt ctggtgccg gaccccgtc acggccttc ggcaaaagg aaaacggtg gaggaagtc tttcgaccc gaccgaaaa aatattctt tccccgaac gaaagagcc aaaatcaaa ccgaaaatc ctcctcgaa ccgcccaaa ccctaccta tcggcgcgc gacatcaac agagaaaaa attctcaat tcgattaaa gtcagagag agtcagtta aacgactcc atggacgtg cttttgccc agactgcac aaagtcgcc gaaaacccc aatctcccc ggatgttgg gattgtgtt tgttgtcct cagagtccg agacattac ctggtgcgc gactcgcca gaccgcagc cgtcgcgct gccgaagag gtctattcg tactcgtat tctgcggaa tcgagccgc tctggtgac ccttactca cgtccaagc acccgttct tataccacg agcgagagt gtaaatcgt tcctcgaat tctgggcca ggttcttac tcttacagc actgaaagg tcctccagg agctacggg gacgggcct gggggctac cactccagc tacagctcg agcacctcc ggacgactc ccagggggc acaacctac cgccactat tcttaccgc tccgcccca tcaagacaa gtaaggatt cttcccgga ctcggtaaa gttcatgtg gtccacacc tacgaccgc atcgtacca tatgtgggt cacaaaaga ttgaccgtg gtaacgcta ccaccaaaa atttactca acacggtcg tccgtccta caaagggag tatgactgg atcgaaaat aaagtccgg ccctgggtt gcctattcc cccacaaac cgttacctc aactctgac tcagctgtg agatacgtc tatgttcgc aatccaata actggctta aagtatcat tcagactat tatccgtct cgatattac aacttttat cgaataatt cgcccgctt tcgtattac gacgattac tggccgttt tataggccg ttaagtgcg tattacagt tactggcct atttctcgt tcagttagc tatctcgat gatattccg ctttatcgt tctcttaga ttacagagt atatttgac gacgaaacg aggctgatt cgagctcag acggcgtct ctcctcaaa caggtccat cagccggta ccgagaatc agaacctgg cccattact ccgttaaac agattcggc gatttcccg tcgttgccg atgaaatac agcaacgac acttacatt cacagactc ttgacttat tccccaaat cacaaaata caatacgcc acatattac acggaaccc gttaaaaaa tacatcggg gacaaaggc tacagccgc cgtcgtcct ctgaccatg tttgaggac gctctcagg aatgatatc cagttgttg agttattac attactaag tttcgagat gagaaacaa caaccgaaa gaaattaaa gcaccttta tcgccagct cgtccttca cgtattttc tcagttcac aagccaatg gaagatccg gtcgctcaa gaaattaaa gatttgcgt gaagcacgg gcacagcgt ctcgctaaa atctacgca tctgatgat aaaacaaca gatgaagta gagaaaagt ctgaaggaa aagaaaagg aaagaagaa gcgaggtta gctgaagaa agagcaatt gctgaagag caagcgaag aaggaagcc gaagcggcc agaaaagca gaaatagct cgtcaagaa cagcttgca gctcaactc gccgcagaa cgtgcagca gaattagag aggcaggcg gaacaagca cgtcaggaa gaactggcc aaacaagct gagattgag cgaatgaag agagaggag gaggaaagg gcgcgcttg gaggaacag cgccgactt gaggaagaa gccaaagct gaagaagag cgccagcaa ctgctccgt ttggaggaa atcgctaga caagccgag gaggaaaaa gagcgagag ttggcgaga caagccgaa gaactggcg gagttagcg agacaagag gccgaacga gcggagcag gaacgtctc gaaagggaa caaaaggag aaggaacag aaggaagca gaacttgca gaactggcg agacaggaa accgaactg gccgaactg gaaagacag gaaaaggag ctggcggaa ctggcggcg aaagcgcaa gaagaactg gccgccaat gaagaagag gaagaaaag actgaagaa caagctgaa ccggtcgtt gatgaacct gaagctgaa gcacaagaa gaagaaccg gctgaagtc gagccggaa gacgagcct gaagccgaa gcagaagcc gatgaataa
- >TC033609 RA
agaaactaa tttgttaag cgctaagtt caaaatagt tcatttaag gaaaaataa tataaggat taatatcca aatgaaagt gggcgtatt tttggccct ttgtgcatc cgttcgtga caaacccac gaaaataag ccgcacaat cgtgcgtcc gattcacga tttaaaaat agccctcct gagcgttgt taagcccag gcttgagcc gcgtgaaat cagtccagt tgatcagat cttgagacg ccgatcgtc agtcctcga acccaccat ggtatacga atccgactt ctacacgac cagaaggcc ctaccgccc ttcgtactc gacgtacag cgtatcgaa acgcgagat tccgtggga gaaagtgcc ttttgtccc aagaccaag tctggtgcc ggaccccgt cacggcctt cggcaaaag gaaaacggt ggaggaagt ctttcgacc cgaccgaaa aaatattct ttccccgaa cgaaagagc caaaatcaa accgaaaat cctcctcga accgcccaa accctacct atcggcgcg cgacatcaa cagagaaaa aattctcaa ttcgattaa agtcagaga gagtcagtt aaacgactc catggacgt gcttttgcc cagactgca caaagtcgc cgaaaaccc caatctccc cggatgttg ggattgtgt ttgttgtcc tcagagtcc gagacatta cctggtgcg cgactcgcc agaccgcag ccgtcgcgc tgccgaaga ggtctattc gtactcgta ttctgcgga atcgagccg ctctggtga cccttactc acgtccaag cacccgttc ttataccac gagcgagag tgtaaatcg ttcctcgaa ttctgggcc aggttctta ctcttacag cactgaaag gtcctccag gagctacgg ggacgggcc tgggggcta ccactccag ctacagctc gagcacctc cggacgact cccaggggg cacaaccta ccgccacta ttcttaccg ctccgcccc atcaagaca agtaaggat tcttcccgg actcggtaa agttcatgt ggtccacac ctacgaccg catcgtacc atatgtggg tcacaaaag attgaccgt ggtaacgct accaccaaa aatttactc aacacggtc gtccgtcct acaaaggga gtatgactg gatcgaaaa taaagtccg gccctgggt tgcctattc ccccacaaa ccgttacct caactctga ctcagctgt gagatacgt ctatgttcg caatccaat aactggctt aaagtatca ttcagacta ttatccgtc tcgatatta caactttta tcgaataat tcgcccgct ttcgtatta cgacgatta ctggccgtt ttataggcc gttaagtgc gtattacag ttactggcc tatttctcg ttcagttag ctatctcga tgatattcc gctttatcg ttctcttag attacagag tatatttga cgacgaaac gaggctgat tcgagctca gacggcgtc tctcctcaa acaggtcca tcagccggt accgagaat cagaacctg gcccattac tccgttaaa cagattcgg cgatttccc gtcgttgcc gatgaaata cagcaacga cacttacat tcacagact cttgactta ttccccaaa tcacaaaat acaatacgc cacatatta cacggaacc cgttaaaaa atacatcgg ggacaaagg ctacagccg ccgtcgtcc tctgaccat gtttgagga cgctctcag gaatgatat ccagttgtt gagttatta cattactaa gtttcgaga tgagaaaca acaaccgaa agaaattaa agcaccttt atcgccagc tcgtccttc acgtatttt ctcagttca caagccaat ggaagatcc ggtcgctca agaaattaa agatttgcg tgaagcacg ggcacagcg tctcgctaa aatctacgc atctgatga taaaacaac agatgaagt agagaaaag tctgaagga aaagaaaag gaaagaaga agcgaggtt agctgaaga aagagcaat tgctgaaga gcaagcgaa gaaggaagc cgaagcggc cagaaaagc agaaatagc tcgtcaaga acagcttgc agctcaact cgccgcaga acgtgcagc agaattaga gaggcaggc ggaacaagc acgtcagga agaactggc caaacaagc tgagattga gcgaatgaa gagagagga ggaggaaag ggcgcgctt ggaggaaca gcgccgact tgaggaaga agccaaagc tgaagaaga gcgccagca actgctccg tttggagga aatcgctag acaagccga ggaggaaaa agagcgaga gttggcgag acaagccga agaactggc ggagttagc gagacaaga ggccgaacg agcggagca ggaacgtct cgaaaggga acaaaagga gaaggaaca gaaggaagc agaacttgc agaactggc gagacagga aaccgaact ggccgaact ggaaagaca ggaaaagga gctggcgga actggcggc gaaagcgca agaagaact ggccgccaa tgaagaaga ggaagaaaa gactgaaga acaagctga accggtcgt tgatgaacc tgaagctga agcacaaga agaagaacc ggctgaagt cgagccgga agacgagcc tgaagccga agcagaagc cgatgaata aacccagtt taaacaatt tctcatttc taacacaca ttccaaact gtcgtgact caagcattt tgttgccaa tttcacgat agtctggaa tgaaaa
