Status
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Gene information
General information
TC015893 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3981603..3984227
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015893
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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      Quotation: {{ goTerm.quotation }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_02524

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    iBeetle screen iB_02536

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - (3 dpi)
    Days post injection
    Comment: Dead animals lost.

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 3

    previtellogenic egg chamber not present - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality late (L7) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015893 RA
      MPAAVDNSK PDRSEQMWK VLKAHILRE RARKKQERE 
AEVEEERLR KEREAREQQ DVMTLGETR EQISQLESK 
LQKLKEEKH QLFLQLKKV LNEDDNRRR QKENNDVLA 
IQALPTGLV HHQPLYISP SVPRNAVPQ QQTFKTAVK 
RPRSPSPQP APVSSYHQG YGYKPSVAV SSYQNSPQK 
DDGRRNELV RAVLWNKTQ YPSQQATFY SVSPQDISM 
YYPLGSISS REPPPSRSA YEPPRPQTY HLEQKLPEH 
YTLRVPSSH GHVIHGGSI PLSPAQPKT GSITAGYPV 
RSQQQYQQS APASLYTAQ SRPLYQSPG TPMNYPPRE
    CDS sequence
    • >TC015893 RA
      atgcctgcc gctgtggac aacagcaag ccggatcgg 
tccgagcag atgtggaag gtgctcaaa gcgcacatt 
ctgcgtgag cgcgctcgc aaaaagcaa gaacgggag 
gccgaagtg gaagaggaa cgtctccgt aaagaacgc 
gaagctcgc gagcaacaa gacgtcatg actttgggt 
gaaacccgc gaacagatc tcacagctt gagagcaag 
ctccagaaa ctgaaagag gagaaacac cagttgttt 
ttacagttg aaaaaagtc ctcaacgag gatgataat 
aggaggcga cagaaggag aacaatgac gtgctggcg 
attcaagcc ctcccgacg ggtctggtg caccaccag 
ccgttgtac atctcgccc agtgtgccc cgcaatgct 
gtgccccag caacaaacg tttaaaact gcggtcaaa 
cgcccgcgg agccctagc ccgcagccg gcccccgtg 
tcgtcctac caccaaggc tatggctac aagccctca 
gtggcggtc tcttcctac cagaactcg ccccaaaaa 
gatgatggt agacgtaat gagttagtg agggcagtt 
ttatggaac aagacgcaa tatccgagt cagcaagca 
acgttttat tcggtctct ccgcaggat atttcgatg 
tattatcct ttggggtcc atctcgtcg cgggagccc 
ccaccctct aggagcgcc tacgagccc ccacggccc 
caaacgtac cacttggag cagaaatta cccgaacat 
tacactcta agagtaccg agttcgcac ggacacgtc 
attcatggg ggctccata cccttgagc cccgcgcag 
cccaagact gggagcatc acagcgggg taccccgtc 
aggagccaa caacaatac caacaatca gcccccgcg 
tcactttat acggctcaa agtcggccg ttgtatcaa 
tcgccaggg actccaatg aattatcct ccaagagaa 
taa
    mRNA sequence
    • >TC015893 RA
      tgtcaaacg tcatgttga cattgacag ttcctgtaa 
aataacaat acgaaataa ttcgtaatt tgtgcaaaa 
tgcctgccg ctgtggaca acagcaagc cggatcggt 
ccgagcaga tgtggaagg tgctcaaag cgcacattc 
tgcgtgagc gcgctcgca aaaagcaag aacgggagg 
ccgaagtgg aagaggaac gtctccgta aagaacgcg 
aagctcgcg agcaacaag acgtcatga ctttgggtg 
aaacccgcg aacagatct cacagcttg agagcaagc 
tccagaaac tgaaagagg agaaacacc agttgtttt 
tacagttga aaaaagtcc tcaacgagg atgataata 
ggaggcgac agaaggaga acaatgacg tgctggcga 
ttcaagccc tcccgacgg gtctggtgc accaccagc 
cgttgtaca tctcgccca gtgtgcccc gcaatgctg 
tgccccagc aacaaacgt ttaaaactg cggtcaaac 
gcccgcgga gccctagcc cgcagccgg cccccgtgt 
cgtcctacc accaaggct atggctaca agccctcag 
tggcggtct cttcctacc agaactcgc cccaaaaag 
atgatggta gacgtaatg agttagtga gggcagttt 
tatggaaca agacgcaat atccgagtc agcaagcaa 
cgttttatt cggtctctc cgcaggata tttcgatgt 
attatcctt tggggtcca tctcgtcgc gggagcccc 
caccctcta ggagcgcct acgagcccc cacggcccc 
aaacgtacc acttggagc agaaattac ccgaacatt 
acactctaa gagtaccga gttcgcacg gacacgtca 
ttcatgggg gctccatac ccttgagcc ccgcgcagc 
ccaagactg ggagcatca cagcggggt accccgtca 
ggagccaac aacaatacc aacaatcag cccccgcgt 
cactttata cggctcaaa gtcggccgt tgtatcaat 
cgccaggga ctccaatga attatcctc caagagaat 
aagaaataa aaattaata actaactaa ttcatttat 
tttcaattt gtttcctat catcgccaa ctgtgacaa 
tttctttag ttccgctcc gaagcgtaa aaactaccg 
gaacttcgg ccaacaccg gattatcct tttccaaat 
gccgaatcc acgaaacta gtgagtctt tactggagg 
tcccagtag cacatagtg ccagaatag taccatctt 
cttgctcat ggacacttg gtaccactg tcagatttt 
tcacgggtg gcttgatgt tgaaaaagg ggattttaa 
tgtcactga catcatcta gatcctctt tgattggag 
ctacgaatt tggcgtttt tgcgcaagg tgaagtaag 
tcacttgtt ggtgcttca gccagacgc gaaagggcc 
cctcgacgt acaaaggct ccgtcacgt cgtgtctct 
tcaacaggt ccttctgga tgggggctt gcgcgctaa 
taatccacg tatcgtcca cacaatcct cgatgtctt 
tactctgat acgtcgtaa cttgtgtct ggatgtcca 
cactgctga tccgctcaa ttgcgcact ttgccagct 
cgtacacgc cgtcaggga gcgggttcg ggatgggcc 
aaggcgacg cgtttttaa atttgggca tccagtaca 
tcatccgcc aatatttgc gcaagggga atccccgat 
ttccgaaaa tatttttca aaagctcct ccatgtcgt 
agtcgggca tcacagcgt tgtcttcca tttgttcga 
gtaagtcga taatgcact gctgctgct tggggtagt 
gcatgaact cgacttgaa acatgttcc tcggaatga 
atttgcctt tcggcagga cgtctatta gggctttgt 
acacttcaa ggtccttgt ggaccccaa aactttcca 
tgtttttta gcgcagagt agataaatt caacgtggc 
cccgccggt ggacactgt ctcggttga cgaaaatgt 
ggatcatgt ccaagtatg tgttcttgt tctttgttt 
cgatttctt caaacgagc ggtaggtca ccagttgct 
tgggctcgg acgaatcgc taaatgttc gcgcaaggg 
ggcccactt ttgggacaa tgcgcaagt tgccccgca 
tcaggggca caagcctgg tcacgaaca ttttaactc 
gtttattta atcgttaaa attggcata cttgccacc 
ggtttgata aaattcgag acttcgggt ttataggtt 
atgtaattt tgaggttac gttgtcacc acattttaa 
aatacaatt aaaaatgaa at

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY