General information | |||
TC015893 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007422.5: 3981603..3984227 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC015893 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
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GO ID | * {{ blurIdResult }} |
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Evidence | {{ blurEvidenceResult }} |
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Reference | * {{ blurReferenceResult }} |
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Quotation | ||
Name | ||
Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02524 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_02536 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - (3 dpi)Comment: Dead animals lost. |
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
previtellogenic egg chamber not present - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC015893 RA
MPAAVDNSK PDRSEQMWK VLKAHILRE RARKKQERE AEVEEERLR KEREAREQQ DVMTLGETR EQISQLESK LQKLKEEKH QLFLQLKKV LNEDDNRRR QKENNDVLA IQALPTGLV HHQPLYISP SVPRNAVPQ QQTFKTAVK RPRSPSPQP APVSSYHQG YGYKPSVAV SSYQNSPQK DDGRRNELV RAVLWNKTQ YPSQQATFY SVSPQDISM YYPLGSISS REPPPSRSA YEPPRPQTY HLEQKLPEH YTLRVPSSH GHVIHGGSI PLSPAQPKT GSITAGYPV RSQQQYQQS APASLYTAQ SRPLYQSPG TPMNYPPRE
- >TC015893 RA
atgcctgcc gctgtggac aacagcaag ccggatcgg tccgagcag atgtggaag gtgctcaaa gcgcacatt ctgcgtgag cgcgctcgc aaaaagcaa gaacgggag gccgaagtg gaagaggaa cgtctccgt aaagaacgc gaagctcgc gagcaacaa gacgtcatg actttgggt gaaacccgc gaacagatc tcacagctt gagagcaag ctccagaaa ctgaaagag gagaaacac cagttgttt ttacagttg aaaaaagtc ctcaacgag gatgataat aggaggcga cagaaggag aacaatgac gtgctggcg attcaagcc ctcccgacg ggtctggtg caccaccag ccgttgtac atctcgccc agtgtgccc cgcaatgct gtgccccag caacaaacg tttaaaact gcggtcaaa cgcccgcgg agccctagc ccgcagccg gcccccgtg tcgtcctac caccaaggc tatggctac aagccctca gtggcggtc tcttcctac cagaactcg ccccaaaaa gatgatggt agacgtaat gagttagtg agggcagtt ttatggaac aagacgcaa tatccgagt cagcaagca acgttttat tcggtctct ccgcaggat atttcgatg tattatcct ttggggtcc atctcgtcg cgggagccc ccaccctct aggagcgcc tacgagccc ccacggccc caaacgtac cacttggag cagaaatta cccgaacat tacactcta agagtaccg agttcgcac ggacacgtc attcatggg ggctccata cccttgagc cccgcgcag cccaagact gggagcatc acagcgggg taccccgtc aggagccaa caacaatac caacaatca gcccccgcg tcactttat acggctcaa agtcggccg ttgtatcaa tcgccaggg actccaatg aattatcct ccaagagaa taa
- >TC015893 RA
tgtcaaacg tcatgttga cattgacag ttcctgtaa aataacaat acgaaataa ttcgtaatt tgtgcaaaa tgcctgccg ctgtggaca acagcaagc cggatcggt ccgagcaga tgtggaagg tgctcaaag cgcacattc tgcgtgagc gcgctcgca aaaagcaag aacgggagg ccgaagtgg aagaggaac gtctccgta aagaacgcg aagctcgcg agcaacaag acgtcatga ctttgggtg aaacccgcg aacagatct cacagcttg agagcaagc tccagaaac tgaaagagg agaaacacc agttgtttt tacagttga aaaaagtcc tcaacgagg atgataata ggaggcgac agaaggaga acaatgacg tgctggcga ttcaagccc tcccgacgg gtctggtgc accaccagc cgttgtaca tctcgccca gtgtgcccc gcaatgctg tgccccagc aacaaacgt ttaaaactg cggtcaaac gcccgcgga gccctagcc cgcagccgg cccccgtgt cgtcctacc accaaggct atggctaca agccctcag tggcggtct cttcctacc agaactcgc cccaaaaag atgatggta gacgtaatg agttagtga gggcagttt tatggaaca agacgcaat atccgagtc agcaagcaa cgttttatt cggtctctc cgcaggata tttcgatgt attatcctt tggggtcca tctcgtcgc gggagcccc caccctcta ggagcgcct acgagcccc cacggcccc aaacgtacc acttggagc agaaattac ccgaacatt acactctaa gagtaccga gttcgcacg gacacgtca ttcatgggg gctccatac ccttgagcc ccgcgcagc ccaagactg ggagcatca cagcggggt accccgtca ggagccaac aacaatacc aacaatcag cccccgcgt cactttata cggctcaaa gtcggccgt tgtatcaat cgccaggga ctccaatga attatcctc caagagaat aagaaataa aaattaata actaactaa ttcatttat tttcaattt gtttcctat catcgccaa ctgtgacaa tttctttag ttccgctcc gaagcgtaa aaactaccg gaacttcgg ccaacaccg gattatcct tttccaaat gccgaatcc acgaaacta gtgagtctt tactggagg tcccagtag cacatagtg ccagaatag taccatctt cttgctcat ggacacttg gtaccactg tcagatttt tcacgggtg gcttgatgt tgaaaaagg ggattttaa tgtcactga catcatcta gatcctctt tgattggag ctacgaatt tggcgtttt tgcgcaagg tgaagtaag tcacttgtt ggtgcttca gccagacgc gaaagggcc cctcgacgt acaaaggct ccgtcacgt cgtgtctct tcaacaggt ccttctgga tgggggctt gcgcgctaa taatccacg tatcgtcca cacaatcct cgatgtctt tactctgat acgtcgtaa cttgtgtct ggatgtcca cactgctga tccgctcaa ttgcgcact ttgccagct cgtacacgc cgtcaggga gcgggttcg ggatgggcc aaggcgacg cgtttttaa atttgggca tccagtaca tcatccgcc aatatttgc gcaagggga atccccgat ttccgaaaa tatttttca aaagctcct ccatgtcgt agtcgggca tcacagcgt tgtcttcca tttgttcga gtaagtcga taatgcact gctgctgct tggggtagt gcatgaact cgacttgaa acatgttcc tcggaatga atttgcctt tcggcagga cgtctatta gggctttgt acacttcaa ggtccttgt ggaccccaa aactttcca tgtttttta gcgcagagt agataaatt caacgtggc cccgccggt ggacactgt ctcggttga cgaaaatgt ggatcatgt ccaagtatg tgttcttgt tctttgttt cgatttctt caaacgagc ggtaggtca ccagttgct tgggctcgg acgaatcgc taaatgttc gcgcaaggg ggcccactt ttgggacaa tgcgcaagt tgccccgca tcaggggca caagcctgg tcacgaaca ttttaactc gtttattta atcgttaaa attggcata cttgccacc ggtttgata aaattcgag acttcgggt ttataggtt atgtaattt tgaggttac gttgtcacc acattttaa aatacaatt aaaaatgaa at
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
