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Gene information
General information
TC015891 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 3964543..3968913
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015891
    iBeetle screen iB_02523

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    larva appendages shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva appendages shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015891 RA
      MASERETII VPGKKILLD VFLVALVGF PILFIFLWL 
      TPYRRGFFC NDEDLMHPY HPSTVPSIY LYIVGIIMN 
      CTVMILIEF LNQPQTSHE VTFWKFRVP FWFYNVYYV 
      LLVFAFGMA CSQLSTDIM KYTVGRLRP HFLTVCKPD 
      INCSLPSNQ HVYHVDFNC TNKDYIHNA RIMKEMRLS 
      FPSGHSSFS MYTMLYFAI YLQRRMDWD GSKLLKHML 
      QFLAVAAAV FVAMTRISD NKHHWSDVL SGLVIGTIV 
      ACVTARVFS TLFQQHPTQ DLVRKDFLE LQGVNGNNA 
      QTA
    CDS sequence
    • >TC015891 RA
      atggcaagt gaacgtgag actataatt gtccccgga 
      aagaaaata ctcttggac gtgtttcta gtggcatta 
      gttggtttc ccgattttg tttattttt ttatggctc 
      actccgtat cgtcggggc tttttttgc aacgacgag 
      gaccttatg cacccatat catccctcg acggtgcca 
      agcatctat ctgtacatt gtgggcatt atcatgaat 
      tgtactgtg atgatcctg atcgaattc ctgaaccag 
      ccgcaaacc agccacgaa gtgactttc tggaaattc 
      cgcgtcccc ttctggttc tacaacgtc tactacgtc 
      cttcttgtt ttcgccttc ggcatggcg tgctcgcaa 
      ttaagcacc gacatcatg aagtacaca gtgggccgt 
      ctccgccct catttcctc acagtctgc aagcccgac 
      atcaactgc tcgctgccc tcaaaccag cacgtctac 
      cacgtggat ttcaactgc accaacaag gactacatc 
      cacaacgcc aggatcatg aaagagatg agactgtca 
      ttcccttca ggacattcc tctttctcc atgtacaca 
      atgctctat tttgctatt tatctgcaa aggagaatg 
      gactgggac ggctccaag ctcttgaag cacatgctg 
      cagtttttg gccgttgcg gccgccgtt tttgtcgca 
      atgacgcgc atttctgac aacaaacac cactggagc 
      gatgttcta agcggactg gttattggg accattgtg 
      gcgtgtgtt acggctcgt gttttttca acgctgttt 
      caacaacac cccacacag gatttggta aggaaggat 
      tttcttgaa ctgcaagga gttaacgga aataacgcc 
      caaaccgcg tag
    mRNA sequence
    • >TC015891 RA
      ctgtggtct ctgtgcagc acacagggt ttggtgtta 
      tgtgttatt taatcatcg aaaatgcca attaatcgc 
      ccgtttaaa cgtgaagtg agggagcgg tttagatca 
      attcagccc aattaatta ttgttacca aattattat 
      gataattcg ttgctaata aataattaa ataaaagag 
      tttgaattg aattgggcg ataaaagac attcctatg 
      acactttta gtactcgct gagtgaatt ttgcgtgga 
      aaaatggca agtgaacgt gagactata attgtcccc 
      ggaaagaaa atactcttg gacgtgttt ctagtggca 
      ttagttggt ttcccgatt ttgtttatt tttttatgg 
      ctcactccg tatcgtcgg ggctttttt tgcaacgac 
      gaggacctt atgcaccca tatcatccc tcgacggtg 
      ccaagcatc tatctgtac attgtgggc attatcatg 
      aattgtact gtgatgatc ctgatcgaa ttcctgaac 
      cagccgcaa accagccac gaagtgact ttctggaaa 
      ttccgcgtc cccttctgg ttctacaac gtctactac 
      gtccttctt gttttcgcc ttcggcatg gcgtgctcg 
      caattaagc accgacatc atgaagtac acagtgggc 
      cgtctccgc cctcatttc ctcacagtc tgcaagccc 
      gacatcaac tgctcgctg ccctcaaac cagcacgtc 
      taccacgtg gatttcaac tgcaccaac aaggactac 
      atccacaac gccaggatc atgaaagag atgagactg 
      tcattccct tcaggacat tcctctttc tccatgtac 
      acaatgctc tattttgct atttatctg caaaggaga 
      atggactgg gacggctcc aagctcttg aagcacatg 
      ctgcagttt ttggccgtt gcggccgcc gtttttgtc 
      gcaatgacg cgcatttct gacaacaaa caccactgg 
      agcgatgtt ctaagcgga ctggttatt gggaccatt 
      gtggcgtgt gttacggct cgtgttttt tcaacgctg 
      tttcaacaa caccccaca caggatttg gtaaggaag 
      gattttctt gaactgcaa ggagttaac ggaaataac 
      gcccaaacc gcgtagttt ttcaagcga cctgatttg 
      tacttaatc atatcacat cacgattat ttatttgtt 
      aaattgaga ttgttttat atttattta tttattgtt 
      taggtgtta agactcgat agcaataaa ttgttagac 
      agtattgag aaacatcgg gaaatgtga tagacctaa 
      ttttgtacc tcgcactcg gtagaaagt accgccaac 
      tcgtgtttg atatttttg tattcataa attgtgata 
      atttgctgg ccaggcccc aataagttg agttgtaac 
      caattattt taataaatt tttccaatt tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY