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Gene information
General information
TC015835 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 9162878..9166177
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC015835
    iBeetle screen iB_02520

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: in most but not all cases the anterior abdomen is constricted.
    larva partially fragmented - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015835 RA
      MSMVSQNQE ECEYGLKDV FKSPKMTKE YLLNHCKEL 
KLYRTPHLN EVLYLHFKG FSYIENLEE YTGLKCLWL 
ENNGIRRIS GLENQKELR SLFLHYNLI QKIENLEHC 
AILDNLNLS HNQVKKIEN LDTIKTLHS LNLSHNYLE 
TIGDIEHLV QLLELSVLD LSNNHLDDP LIVEIMGKM 
PELRVLNLM GNPAVRKIP AYRKTMILA CKNLQYLDD 
RPVFPRDRA CAEAWGRGG VTEENAERQ RWIEREHRK 
ITESVDALI AMRDRRRAE MAQNSHGDS GMGTSVADS 
ESEDKGDEE SSDSEEEQG NNFMEERET EDDYSEYRE 
RIFDFDAKV AKKRKLVEE IEEEDKAKV ENHDLDVEK 
LQERLTEEV GREDMGECL KNMMRAEEF EESEAQEDD 
NKSEGLDTI QEEREKDDG NPKIVEEKD ENGSDTREE 
ELEEAGNAK NDEETYGTA SETNNEFEE RSESVEKHE 
TEDCSEKMS DNVSFGEDM TTESNEENL VKNEEAPEN 
IGDIGKNEE TTTEEVNNL GRKLIETLR KMATEMDND 
KNETRRNLV EVAKETNQE AKPEEVNED SEEELRYYE 
ALDISSSTD FEHLLDRNG SHAKLKTVE RFLTKEDIP 
VPPYVEELD RANPGQEYR EILDWNLKI KEENTKILL 
PIRRRRIEV DEEDELYEL IQEMKKDEE EIQDKSEDN 
SKAPSNPNF VEDTMNECG LLAQSIASL RKDMAEFCD 
SIGEFLAKT SGKKGNLMK QVEAEEEEE ENDEFEECE 
EVVNESDEN EVNPIVKRK VTQTLEMQV VNEM
    CDS sequence
    • >TC015835 RA
      atgtcgatg gtgagccaa aatcaggaa gaatgcgaa 
tatgggctg aaagacgtg ttcaaaagc cccaagatg 
acgaaggag tacctcctc aaccactgc aaggagctg 
aaactgtac cgcaccccg catttgaac gaagtcctt 
tatttgcac tttaagggc ttctcgtac attgagaat 
ttggaggag tacaccggc ctgaaatgc ctctggttg 
gagaacaac ggaattcgc agaatcagt ggtttggag 
aaccaaaaa gaactgcgc agcttgttc ctccactac 
aacctgatc caaaaaatc gaaaacttg gagcactgc 
gccatcctc gacaattta aacttgtcg cacaaccag 
gtgaagaaa atcgagaat ttggacacg atcaaaaca 
ctacatagc ttgaatttg tcgcacaat tatttggaa 
acaatcggg gatattgaa catttggtc cagctcctc 
gaattgtca gtcctcgac ttgtcgaac aaccatctc 
gatgaccct ctgattgtt gaaataatg ggcaaaatg 
cccgaactg cgcgtttta aatttgatg ggaaacccc 
gcagttagg aaaatacca gcgtaccgc aaaaccatg 
attttggct tgtaaaaat ctgcaatat ttggacgac 
cgaccggtg tttccccga gaccgcgca tgcgccgaa 
gcttggggc cgtggcggt gtgactgag gaaaatgcg 
gaaagacag cgttggatc gaaagagaa catcgcaaa 
ataactgaa agtgtcgat gccttgata gcaatgagg 
gatcgaaga agagccgaa atggcgcaa aattcacat 
ggggatagt ggcatgggt acgtctgtt gcagactcc 
gagagtgaa gataaaggt gatgaagaa agtagcgat 
agtgaagaa gaacaaggc aataatttt atggaggaa 
agagaaacg gaagatgat tattccgaa taccgagaa 
cgaatcttt gattttgat gcaaaagtt gcgaagaaa 
aggaagcta gttgaagaa atagaagaa gaagataag 
gcgaaagtg gaaaatcat gacttggac gtggagaaa 
ctgcaagaa agactgacg gaagaagtc ggaagggaa 
gatatggga gagtgtttg aagaatatg atgcgcgct 
gaagagttt gaagaaagt gaagctcag gaagacgat 
aataagagt gaagggctt gatactatt caagaagaa 
agggagaaa gatgatggt aatccaaaa attgttgag 
gaaaaagat gaaaatgga agtgatacc agagaagaa 
gaattggag gaagcagga aatgcaaag aacgatgaa 
gaaacttat ggaacagcg tccgaaact aacaatgag 
tttgaagaa aggtcagaa agtgtggaa aaacatgaa 
acagaagac tgctctgaa aaaatgagt gacaatgtc 
agttttggc gaagacatg acaactgaa tcgaacgaa 
gaaaacttg gtaaaaaat gaagaagca cccgaaaat 
atcggtgat attgggaag aacgaggaa acgacaacc 
gaagaagta aataatttg gggcgtaaa ttaattgaa 
actcttcgt aaaatggca accgagatg gacaatgac 
aagaatgag acaagaaga aacctagtc gaagtggct 
aaagaaacg aatcaagag gcgaaacca gaagaagta 
aatgaagat tctgaagaa gaattacgt tattatgaa 
gcgctagac atctcaagc agcaccgac tttgagcat 
cttttggac cgaaacgga agccacgca aaacttaaa 
actgttgag agatttctc actaaggaa gatattcct 
gtaccgcct tacgtcgag gagttggat agagccaat 
cctgggcaa gagtacagg gagatactc gactggaat 
ttgaagatc aaagaagaa aacacaaaa attttgctc 
ccgattcgt agaagaaga atcgaagtg gatgaagaa 
gacgagttg tacgaactc attcaagaa atgaagaaa 
gatgaagaa gagatacaa gacaagagt gaagataat 
tccaaagct ccatctaat ccaaatttt gttgaagat 
acgatgaac gagtgtgga ttgttggca cagtcgata 
gctagtttg aggaaagat atggcggag ttctgtgac 
agtattggg gagttttta gctaaaaca agtggtaag 
aaggggaat ttgatgaag caagttgaa gcagaagaa 
gaagaagaa gaaaatgat gaattcgaa gagtgtgaa 
gaagtggtc aatgaaagt gacgaaaat gaagtcaat 
cccattgtg aaacgcaag gttacacaa actctagaa 
atgcaagtt gtaaacgag atgtaa
    mRNA sequence
    • >TC015835 RA
      ataatttgc aaaaatgtc gatggtgag ccaaaatca 
ggaagaatg cgaatatgg gctgaaaga cgtgttcaa 
aagccccaa gatgacgaa ggagtacct cctcaacca 
ctgcaagga gctgaaact gtaccgcac cccgcattt 
gaacgaagt cctttattt gcactttaa gggcttctc 
gtacattga gaatttgga ggagtacac cggcctgaa 
atgcctctg gttggagaa caacggaat tcgcagaat 
cagtggttt ggagaacca aaaagaact gcgcagctt 
gttcctcca ctacaacct gatccaaaa aatcgaaaa 
cttggagca ctgcgccat cctcgacaa tttaaactt 
gtcgcacaa ccaggtgaa gaaaatcga gaatttgga 
cacgatcaa aacactaca tagcttgaa tttgtcgca 
caattattt ggaaacaat cggggatat tgaacattt 
ggtccagct cctcgaatt gtcagtcct cgacttgtc 
gaacaacca tctcgatga ccctctgat tgttgaaat 
aatgggcaa aatgcccga actgcgcgt tttaaattt 
gatgggaaa ccccgcagt taggaaaat accagcgta 
ccgcaaaac catgatttt ggcttgtaa aaatctgca 
atatttgga cgaccgacc ggtgtttcc ccgagaccg 
cgcatgcgc cgaagcttg gggccgtgg cggtgtgac 
tgaggaaaa tgcggaaag acagcgttg gatcgaaag 
agaacatcg caaaataac tgaaagtgt cgatgcctt 
gatagcaat gagggatcg aagaagagc cgaaatggc 
gcaaaattc acatgggga tagtggcat gggtacgtc 
tgttgcaga ctccgagag tgaagataa aggtgatga 
agaaagtag cgatagtga agaagaaca aggcaataa 
ttttatgga ggaaagaga aacggaaga tgattattc 
cgaataccg agaacgaat ctttgattt tgatgcaaa 
agttgcgaa gaaaaggaa gctagttga agaaataga 
agaagaaga taaggcgaa agtggaaaa tcatgactt 
ggacgtgga gaaactgca agaaagact gacggaaga 
agtcggaag ggaagatat gggagagtg tttgaagaa 
tatgatgcg cgctgaaga gtttgaaga aagtgaagc 
tcaggaaga cgataataa gagtgaagg gcttgatac 
tattcaaga agaaaggga gaaagatga tggtaatcc 
aaaaattgt tgaggaaaa agatgaaaa tggaagtga 
taccagaga agaagaatt ggaggaagc aggaaatgc 
aaagaacga tgaagaaac ttatggaac agcgtccga 
aactaacaa tgagtttga agaaaggtc agaaagtgt 
ggaaaaaca tgaaacaga agactgctc tgaaaaaat 
gagtgacaa tgtcagttt tggcgaaga catgacaac 
tgaatcgaa cgaagaaaa cttggtaaa aaatgaaga 
agcacccga aaatatcgg tgatattgg gaagaacga 
ggaaacgac aaccgaaga agtaaataa tttggggcg 
taaattaat tgaaactct tcgtaaaat ggcaaccga 
gatggacaa tgacaagaa tgagacaag aagaaacct 
agtcgaagt ggctaaaga aacgaatca agaggcgaa 
accagaaga agtaaatga agattctga agaagaatt 
acgttatta tgaagcgct agacatctc aagcagcac 
cgactttga gcatctttt ggaccgaaa cggaagcca 
cgcaaaact taaaactgt tgagagatt tctcactaa 
ggaagatat tcctgtacc gccttacgt cgaggagtt 
ggatagagc caatcctgg gcaagagta cagggagat 
actcgactg gaatttgaa gatcaaaga agaaaacac 
aaaaatttt gctcccgat tcgtagaag aagaatcga 
agtggatga agaagacga gttgtacga actcattca 
agaaatgaa gaaagatga agaagagat acaagacaa 
gagtgaaga taattccaa agctccatc taatccaaa 
ttttgttga agatacgat gaacgagtg tggattgtt 
ggcacagtc gatagctag tttgaggaa agatatggc 
ggagttctg tgacagtat tggggagtt tttagctaa 
aacaagtgg taagaaggg gaatttgat gaagcaagt 
tgaagcaga agaagaaga agaagaaaa tgatgaatt 
cgaagagtg tgaagaagt ggtcaatga aagtgacga 
aaatgaagt caatcccat tgtgaaacg caaggttac 
acaaactct agaaatgca agttgtaaa cgagatgta 
attttttag aaataaata ttattcgtt ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY