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Gene information
General information
TC015835 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid43: 3896021..3899320
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015835
    iBeetle screen iB_02520
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    beetle stock: bad (increased lethality),
    Pupal morphology
    beetle stock: bad (increased lethality),
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva constricted - Penetrance (percentage of animals/eggs on slide): less than 30%

    Comment: in most but not all cases the anterior abdomen is constricted.
    larva partially fragmented - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    labrum position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs sometimes shortened - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015835 RA
      MSMVSQNQE ECEYGLKDV FKSPKMTKE YLLNHCKEL 
      KLYRTPHLN EVLYLHFKG FSYIENLEE YTGLKCLWL 
      ENNGIRRIS GLENQKELR SLFLHYNLI QKIENLEHC 
      AILDNLNLS HNQVKKIEN LDTIKTLHS LNLSHNYLE 
      TIGDIEHLV QLLELSVLD LSNNHLDDP LIVEIMGKM 
      PELRVLNLM GNPAVRKIP AYRKTMILA CKNLQYLDD 
      RPVFPRDRA CAEAWGRGG VTEENAERQ RWIEREHRK 
      ITESVDALI AMRDRRRAE MAQNSHGDS GMGTSVADS 
      ESEDKGDEE SSDSEEEQG NNFMEERET EDDYSEYRE 
      RIFDFDAKV AKKRKLVEE IEEEDKAKV ENHDLDVEK 
      LQERLTEEV GREDMGECL KNMMRAEEF EESEAQEDD 
      NKSEGLDTI QEEREKDDG NPKIVEEKD ENGSDTREE 
      ELEEAGNAK NDEETYGTA SETNNEFEE RSESVEKHE 
      TEDCSEKMS DNVSFGEDM TTESNEENL VKNEEAPEN 
      IGDIGKNEE TTTEEVNNL GRKLIETLR KMATEMDND 
      KNETRRNLV EVAKETNQE AKPEEVNED SEEELRYYE 
      ALDISSSTD FEHLLDRNG SHAKLKTVE RFLTKEDIP 
      VPPYVEELD RANPGQEYR EILDWNLKI KEENTKILL 
      PIRRRRIEV DEEDELYEL IQEMKKDEE EIQDKSEDN 
      SKAPSNPNF VEDTMNECG LLAQSIASL RKDMAEFCD 
      SIGEFLAKT SGKKGNLMK QVEAEEEEE ENDEFEECE 
      EVVNESDEN EVNPIVKRK VTQTLEMQV VNEM
    CDS sequence
    • >TC015835 RA
      atgtcgatg gtgagccaa aatcaggaa gaatgcgaa 
      tatgggctg aaagacgtg ttcaaaagc cccaagatg 
      acgaaggag tacctcctc aaccactgc aaggagctg 
      aaactgtac cgcaccccg catttgaac gaagtcctt 
      tatttgcac tttaagggc ttctcgtac attgagaat 
      ttggaggag tacaccggc ctgaaatgc ctctggttg 
      gagaacaac ggaattcgc agaatcagt ggtttggag 
      aaccaaaaa gaactgcgc agcttgttc ctccactac 
      aacctgatc caaaaaatc gaaaacttg gagcactgc 
      gccatcctc gacaattta aacttgtcg cacaaccag 
      gtgaagaaa atcgagaat ttggacacg atcaaaaca 
      ctacatagc ttgaatttg tcgcacaat tatttggaa 
      acaatcggg gatattgaa catttggtc cagctcctc 
      gaattgtca gtcctcgac ttgtcgaac aaccatctc 
      gatgaccct ctgattgtt gaaataatg ggcaaaatg 
      cccgaactg cgcgtttta aatttgatg ggaaacccc 
      gcagttagg aaaatacca gcgtaccgc aaaaccatg 
      attttggct tgtaaaaat ctgcaatat ttggacgac 
      cgaccggtg tttccccga gaccgcgca tgcgccgaa 
      gcttggggc cgtggcggt gtgactgag gaaaatgcg 
      gaaagacag cgttggatc gaaagagaa catcgcaaa 
      ataactgaa agtgtcgat gccttgata gcaatgagg 
      gatcgaaga agagccgaa atggcgcaa aattcacat 
      ggggatagt ggcatgggt acgtctgtt gcagactcc 
      gagagtgaa gataaaggt gatgaagaa agtagcgat 
      agtgaagaa gaacaaggc aataatttt atggaggaa 
      agagaaacg gaagatgat tattccgaa taccgagaa 
      cgaatcttt gattttgat gcaaaagtt gcgaagaaa 
      aggaagcta gttgaagaa atagaagaa gaagataag 
      gcgaaagtg gaaaatcat gacttggac gtggagaaa 
      ctgcaagaa agactgacg gaagaagtc ggaagggaa 
      gatatggga gagtgtttg aagaatatg atgcgcgct 
      gaagagttt gaagaaagt gaagctcag gaagacgat 
      aataagagt gaagggctt gatactatt caagaagaa 
      agggagaaa gatgatggt aatccaaaa attgttgag 
      gaaaaagat gaaaatgga agtgatacc agagaagaa 
      gaattggag gaagcagga aatgcaaag aacgatgaa 
      gaaacttat ggaacagcg tccgaaact aacaatgag 
      tttgaagaa aggtcagaa agtgtggaa aaacatgaa 
      acagaagac tgctctgaa aaaatgagt gacaatgtc 
      agttttggc gaagacatg acaactgaa tcgaacgaa 
      gaaaacttg gtaaaaaat gaagaagca cccgaaaat 
      atcggtgat attgggaag aacgaggaa acgacaacc 
      gaagaagta aataatttg gggcgtaaa ttaattgaa 
      actcttcgt aaaatggca accgagatg gacaatgac 
      aagaatgag acaagaaga aacctagtc gaagtggct 
      aaagaaacg aatcaagag gcgaaacca gaagaagta 
      aatgaagat tctgaagaa gaattacgt tattatgaa 
      gcgctagac atctcaagc agcaccgac tttgagcat 
      cttttggac cgaaacgga agccacgca aaacttaaa 
      actgttgag agatttctc actaaggaa gatattcct 
      gtaccgcct tacgtcgag gagttggat agagccaat 
      cctgggcaa gagtacagg gagatactc gactggaat 
      ttgaagatc aaagaagaa aacacaaaa attttgctc 
      ccgattcgt agaagaaga atcgaagtg gatgaagaa 
      gacgagttg tacgaactc attcaagaa atgaagaaa 
      gatgaagaa gagatacaa gacaagagt gaagataat 
      tccaaagct ccatctaat ccaaatttt gttgaagat 
      acgatgaac gagtgtgga ttgttggca cagtcgata 
      gctagtttg aggaaagat atggcggag ttctgtgac 
      agtattggg gagttttta gctaaaaca agtggtaag 
      aaggggaat ttgatgaag caagttgaa gcagaagaa 
      gaagaagaa gaaaatgat gaattcgaa gagtgtgaa 
      gaagtggtc aatgaaagt gacgaaaat gaagtcaat 
      cccattgtg aaacgcaag gttacacaa actctagaa 
      atgcaagtt gtaaacgag atgtaa
    mRNA sequence
    • >TC015835 RA
      ataatttgc aaaaatgtc gatggtgag ccaaaatca 
      ggaagaatg cgaatatgg gctgaaaga cgtgttcaa 
      aagccccaa gatgacgaa ggagtacct cctcaacca 
      ctgcaagga gctgaaact gtaccgcac cccgcattt 
      gaacgaagt cctttattt gcactttaa gggcttctc 
      gtacattga gaatttgga ggagtacac cggcctgaa 
      atgcctctg gttggagaa caacggaat tcgcagaat 
      cagtggttt ggagaacca aaaagaact gcgcagctt 
      gttcctcca ctacaacct gatccaaaa aatcgaaaa 
      cttggagca ctgcgccat cctcgacaa tttaaactt 
      gtcgcacaa ccaggtgaa gaaaatcga gaatttgga 
      cacgatcaa aacactaca tagcttgaa tttgtcgca 
      caattattt ggaaacaat cggggatat tgaacattt 
      ggtccagct cctcgaatt gtcagtcct cgacttgtc 
      gaacaacca tctcgatga ccctctgat tgttgaaat 
      aatgggcaa aatgcccga actgcgcgt tttaaattt 
      gatgggaaa ccccgcagt taggaaaat accagcgta 
      ccgcaaaac catgatttt ggcttgtaa aaatctgca 
      atatttgga cgaccgacc ggtgtttcc ccgagaccg 
      cgcatgcgc cgaagcttg gggccgtgg cggtgtgac 
      tgaggaaaa tgcggaaag acagcgttg gatcgaaag 
      agaacatcg caaaataac tgaaagtgt cgatgcctt 
      gatagcaat gagggatcg aagaagagc cgaaatggc 
      gcaaaattc acatgggga tagtggcat gggtacgtc 
      tgttgcaga ctccgagag tgaagataa aggtgatga 
      agaaagtag cgatagtga agaagaaca aggcaataa 
      ttttatgga ggaaagaga aacggaaga tgattattc 
      cgaataccg agaacgaat ctttgattt tgatgcaaa 
      agttgcgaa gaaaaggaa gctagttga agaaataga 
      agaagaaga taaggcgaa agtggaaaa tcatgactt 
      ggacgtgga gaaactgca agaaagact gacggaaga 
      agtcggaag ggaagatat gggagagtg tttgaagaa 
      tatgatgcg cgctgaaga gtttgaaga aagtgaagc 
      tcaggaaga cgataataa gagtgaagg gcttgatac 
      tattcaaga agaaaggga gaaagatga tggtaatcc 
      aaaaattgt tgaggaaaa agatgaaaa tggaagtga 
      taccagaga agaagaatt ggaggaagc aggaaatgc 
      aaagaacga tgaagaaac ttatggaac agcgtccga 
      aactaacaa tgagtttga agaaaggtc agaaagtgt 
      ggaaaaaca tgaaacaga agactgctc tgaaaaaat 
      gagtgacaa tgtcagttt tggcgaaga catgacaac 
      tgaatcgaa cgaagaaaa cttggtaaa aaatgaaga 
      agcacccga aaatatcgg tgatattgg gaagaacga 
      ggaaacgac aaccgaaga agtaaataa tttggggcg 
      taaattaat tgaaactct tcgtaaaat ggcaaccga 
      gatggacaa tgacaagaa tgagacaag aagaaacct 
      agtcgaagt ggctaaaga aacgaatca agaggcgaa 
      accagaaga agtaaatga agattctga agaagaatt 
      acgttatta tgaagcgct agacatctc aagcagcac 
      cgactttga gcatctttt ggaccgaaa cggaagcca 
      cgcaaaact taaaactgt tgagagatt tctcactaa 
      ggaagatat tcctgtacc gccttacgt cgaggagtt 
      ggatagagc caatcctgg gcaagagta cagggagat 
      actcgactg gaatttgaa gatcaaaga agaaaacac 
      aaaaatttt gctcccgat tcgtagaag aagaatcga 
      agtggatga agaagacga gttgtacga actcattca 
      agaaatgaa gaaagatga agaagagat acaagacaa 
      gagtgaaga taattccaa agctccatc taatccaaa 
      ttttgttga agatacgat gaacgagtg tggattgtt 
      ggcacagtc gatagctag tttgaggaa agatatggc 
      ggagttctg tgacagtat tggggagtt tttagctaa 
      aacaagtgg taagaaggg gaatttgat gaagcaagt 
      tgaagcaga agaagaaga agaagaaaa tgatgaatt 
      cgaagagtg tgaagaagt ggtcaatga aagtgacga 
      aaatgaagt caatcccat tgtgaaacg caaggttac 
      acaaactct agaaatgca agttgtaaa cgagatgta 
      attttttag aaataaata ttattcgtt ttc